Crops ›› 2025, Vol. 41 ›› Issue (2): 162-171.doi: 10.16035/j.issn.1001-7283.2025.02.023

Previous Articles     Next Articles

Effects of Application Period of Microbial Inoculants on Rhizosphere Soil Bacterial Diversity, Enzyme Activity and Yield and Quality of Flue-Cured Tobacco

Zhang Jili1(), He Jinghao2, Wei Jianyu1, Huang Chongjun1, Wang Wei2, Cai Yixia3()   

  1. 1China Tobacco Guangxi Industrial Co., Ltd., Nanning 530001, Guangxi, China
    2College of Agriculture, South China Agricultural University, Guangzhou 510642, Guangdong, China
    3College of Resources and Environment, South China Agricultural University, Guangzhou 510642, Guangdong, China
  • Received:2023-10-23 Revised:2024-02-23 Online:2025-04-15 Published:2025-04-16

RichHTML

2

PDF (PC)

53

Abstract:

To confirm the period of microbial agent application, this study explored the impact of applying microbial agents at different stages on the soil microbial diversity, enzyme activity, and the yield and quality of flue-cured tobacco. Four treatments were set: application at ridging period (A1), application at transplanting (A2), application at bulk curing stage (A3), and no application (CK). The bacterial community structure under different treatments was sequenced using 16S rDNA. Meanwhile, soil enzyme activity, relevant indicators of flue-cured tobacco production and quality were determined, and field disease conditions were investigated to clarify the relationship among soil, bacteria, and flue-cured tobacco. The results showed that, compared with CK, the application of microbial agents significantly increased the number of Observed species, Shannon index, and Simpson index of observed bacterial communities in the rhizosphere soil. Compared with A1 and A3 treatments, the relative abundance of Proteobacteria, Actinobacteria, Firmicutes, and Gemmatimonadetes were highest in the rhizosphere soil under A2 treatment. At the same time, the relative abundance of Acidobacteria decreased. The activities of polyphenol oxidase, sucrase, urease, and catalase were highest in the rhizosphere soil, and the incidence rate and disease index of tobacco bacterial wilt were significantly decreased. It could also increase the yield, output value, average price, and the proportion of surperior tobacco. The application of microbial inoculants during transplanting had a notable preventive effect against tobacco bacterial wilt. It also effectively enriched the beneficial bacterial community in the rhizospheric soil, enhanced soil enzyme activity, improved the chemical composition of tobacco leaves, and ultimately increased the economic benefits of tobacco cultivation.

Key words: Flue-cured tobacco, Rhizosphere soil, Microbial inoculants, Bacterial diversity, Yield, Quality

Table 1

Effects of microbial inoculant application period on soil enzyme activity U/g"

处理Treatment 多酚氧化酶Polyphenol oxidase 蔗糖酶Sucrase 脲酶Urease 过氧化氢酶Catalase
CK 17.79±0.12c 58.22±1.02c 283.25±2.04d 15.72±0.30c
A1 20.00±0.21b 62.20±0.13b 320.11±1.19c 21.63±1.16b
A2 20.92±0.12a 67.82±0.10a 408.80±0.97a 26.46±1.12a
A3 19.63±0.18b 62.54±0.17b 337.27±1.45b 23.41±1.15ab

Table 2

Analysis of the alpha diversity index of bacterial communities during the application period of microbial agents"

处理
Treatment		 观测到的物种
Observed species		 Chao1指数
Chao1 index		 物种覆盖度
Species coverage		 香农指数
Shannon index		 辛普森指数
Simpson index
CK 3401.50±1.73c 3602.02±170.87c 0.93±0.00 8.36±0.04c 0.86±0.01c
A1 3405.27±3.77c 4105.77±3.75b 0.98±0.00 9.07±0.01b 0.94±0.01b
A2 3881.33±10.23a 4625.90±7.41a 0.98±0.00 9.63±0.13a 0.99±0.01a
A3 3601.73±45.34b 4414.59±39.11a 0.98±0.00 9.30±0.11b 0.92±0.02b

Fig.1

Evolutionary tree of soil bacterial communities in the rhizosphere of tobacco plant"

Fig.2

Effects of the application period of microbial inoculants on the distribution of bacterial community structure in rhizospheric soil"

Fig.3

Cluster diagram of the top ten bacterial communities in the rhizosphere soil of flue-cured tobacco based on horizontal ordination"

Fig.4

Network diagram of horizontally crucial bacteria symbiosis in flue-cured tobacco rhizosphere soil The edges connecting nodes represent their relationships: red edges indicate positive correlations, while green edges indicate negative correlations. Node colors represent their respective phyla."

Fig.5

Topological structure-related indicators of symbiotic network of soil bacterial communities in the rhizosphere of flue-cured tobacco"

Table 3

Effects of application period of microbial agents on the occurrence of bacterial wilt %"

处理
Treatment		 青枯病Bacterial wilt
发病率
Incidence rate		 病情指数
Disease index		 相对防治效果
Relative control effect
CK 66.91±1.00a 38.27±1.03a －
A1 37.96±0.46b 20.19±1.03b 47.25±2.69b
A2 19.02±1.24d 11.16±0.37c 70.82±0.96a
A3 31.64±0.83c 19.10±1.67b 50.08±4.37b

Table 4

Effects of application period of microbial agents on the conventional chemical composition of C3F tobacco leaves"

处理
Treatment		 总氮
Total nitrogen
(%)		 钾含量
K content
(%)		 烟碱
Nicotine
(%)		 还原糖
Reducing
sugar (%)		 总糖
Total
sugar (%)		 两糖差
Disaccharide
difference (%)		 氮碱比
Nitrogen-
alkali ratio		 糖碱比
Sugar-
alkali ratio
CK 1.53±0.05c 2.18±0.02b 1.82±0.11b 14.83±0.78b 21.60±0.84b 6.77±0.22b 0.84±0.39a 11.90±0.62a
A1 1.86±0.06b 2.89±0.17a 2.20±0.10ab 18.54±0.48a 24.34±0.54a 5.80±0.26a 0.85±0.06a 11.11±0.41a
A2 2.13±0.05a 2.88±0.21a 2.48±0.16a 19.61±0.33a 25.56±0.12a 5.96±0.24a 0.86±0.04a 10.37±0.59a
A3 2.02±0.01a 2.90±0.17a 2.46±0.21a 20.09±0.09a 26.18±0.42a 6.09±0.37a 0.83±0.07a 10.81±1.03a

Table 5

Effects of application period of microbial agents on economic traits of flue-cured tobacco"

处理
Treatment		 产量
Yield (kg/hm2)		 产值（元/hm2）
Output value (yuan/hm2)		 均价（元/kg）
Average price (yuan/kg)		 上等烟比例
Superior tobacco percentage (%)		 中等烟比例
Medium tobacco percentage (%)
CK 1929.51±32.89d 51 031.92±1944.98d 26.44±0.70d 35.87±0.50d 41.12±1.46ab
A1 2213.35±25.37c 65 105.02±1858.17c 29.41±0.61c 43.03±1.18c 44.00±1.08a
A2 2491.82±18.68a 84 118.96±1106.29a 33.76±0.56a 56.82±0.46a 39.95±0.12b
A3 2361.84±22.18b 74 962.31±1025.37b 31.74±0.37b 46.89±0.80b 44.03±1.01a

Fig.6

Heatmap depicting the correlations between the composition of rhizosphere soil bacterial communities and rhizosphere soil enzyme activities, status of bacterial wilt disease, and the yield and quality of flue-cured tobacco The figure is a heatmap illustrating the correlations between soil enzyme activities, tobacco black shank disease incidence, tobacco quality, and yield. Orange lines represent highly significant correlations (P < 0.01). Green lines represent significant correlations (P < 0.05). Grey lines represent non-significant correlations."

[1] 施河丽, 向必坤, 彭五星, 等. 有机无机肥料配施对植烟土壤养分及细菌群落结构的影响. 中国土壤与肥料, 2019(4):58-66.
[2] 马宜林, 吴广海, 申洪涛, 等. 羊粪有机肥与化肥配施对烤烟生长及土壤肥力特性的影响. 核农学报, 2021, 35(10):2423-2430.
doi: 10.11869/j.issn.100-8551.2021.10.2423
[3] 谢丹凤, 袁文彬, 谢晋, 等. 不同比例有机肥与氮肥施用量对烤烟产质量及主要化学成分的影响. 江苏农业科学, 2023, 51 (19):87-97.
[4] 刘光辉, 李迪秦, 陈一凡, 等. 烤烟生长发育特性及产质量对施肥技术的响应. 核农学报, 2017, 31(10):2032-2038.
doi: 10.11869/j.issn.100-8551.2017.10.2032
[5] 梁兵, 黄坤, 付亚丽, 等. 不同比例有机肥与化肥配施对烤烟生长及产质量的影响. 西南农业学报, 2017, 30(8):1822-1825.
[6] 付利波, 苏帆, 陈华, 等. 菜籽饼肥不同用量对烤烟产量及质量的影响. 中国生态农业学报, 2007, 15(6):77-80.
[7] 易建华, 张新要, 李天福, 等. 不同有机质土壤饼肥用量对烤烟产量和质量的影响. 中国农学通报, 2006, 22(10):216-220.
[8] 李硕, 秦闯, 魏欢, 等. 设施茄子各生育期施用微生物菌剂对产量及土壤性状的影响. 河北农业大学学报, 2019, 42(6):65-70.
doi: 10.13320/j.cnki.jauh.2019.0123
[9] 韩忠明, 杨颂, 韩梅, 等. 不同菌剂对人参连作土壤酶活性的影响. 东北农业科学, 2016, 41(1):50-53.
[10] 王凌云, 高亮, 李晓, 等. 微生物套餐肥对设施番茄产量和品质的影响. 中国果菜, 2017, 37(6):35-38.
[11] 黄光祥, 董丽红, 马斌, 等. 3种微生物菌剂在云烟99上的应用效果. 湖南农业科学, 2023(4):45-49.
[12] 孙真, 郑亮, 邱浩斌. 植物根际促生细菌定殖研究进展. 生物技术通报, 2017, 33(2):8-15.
doi: 10.13560/j.cnki.biotech.bull.1985.2017.02.002
[13] 李乾云, 张剑飞, 李莞晴, 等. 牡丹江烟区植烟土壤养分时空变化特征. 贵州农业科学, 2022, 50(9):54-61.
[14] 阎海涛, 常栋, 许跃奇, 等. 生物炭和微生物菌剂对烤烟产质量的影响. 贵州农业科学, 2021, 49(10):21-27.
[15] 国家质量监督检验检疫总局.烟草病虫害分级及调查方法:GB/T 23222-2008. 北京: 中国标准出版社, 2009.
[16] 国家质量监督检验检疫总局. 烤烟:GB 2635-1992. 北京: 中国标准出版社, 1992.
[17] 国家烟草专卖局. 烟草及烟草制品水溶性糖的测定连续流动法:YC/T 159-2019. 北京: 中国标准出版社, 2019.
[18] 国家烟草专卖局. 烟草及烟草制品淀粉的测定连续流动法:YC/T 216-2013. 北京: 中国标准出版社, 2014.
[19] 国家烟草专卖局.烟草及烟草制品总植物碱的测定连续流动法:YC/T 160-2002. 北京: 中国标准出版社, 2004.
[20] 国家烟草专卖局.烟草及烟草制品总植物总氮的测定连续流动法:YC/T 161-2002. 北京: 中国标准出版社, 2004.
[21] 国家烟草专卖局. 卷烟纸中钾、钠、钙、镁的测定火焰原子吸收光谱法:YC/T 274-2008. 北京: 中国标准出版社, 2009.
[22] 国家烟草专卖局.烟草及烟草制品石油醚提取物的测定:YC/T 176-2003. 北京: 中国标准出版社, 2004.
[23] Ma T F, He X H, Chen S G, et al. Long-term organic-inorganic fertilization regimes alter bacterial and fungal communities and rice yields in paddy soil. Frontiers in Microbiology, 2022,13:890712.
[24] Wang J F, Zheng J Y, Shi W Y, et al. Dysbiosis of maternal and neonatal microbiota associated with gestational diabetes mellitus. Gut, 2018, 67(9):1614-1625.
doi: 10.1136/gutjnl-2018-315988 pmid: 29760169
[25] 吴昌昊, 刘敬泽. 根际微生物影响因素及其与植物互作研究进展. 河北师范大学学报(自然科学版), 2022, 46(6):603-613.
[26] 唐杰, 陈知青, 郭安南, 等. 不同作物根际土壤微生物的群落结构特征分析. 核农学报, 2021, 35(12):2830-2840.
doi: 10.11869/j.issn.100-8551.2021.12.2830
[27] 祝蕾, 严辉, 刘培, 等. 药用植物根际微生物对其品质形成的影响及其作用机制的研究进展. 中草药, 2021, 52(13):4064-4073.
[28] 吕柏辰, 孙海, 钱佳奇. 等. 药用植物根系分泌物与根际微生物相互作用及其在中药材生态种植中的应用. 中国中药杂志, 2024, 49(8):2128-2137.
[29] 夏晶晶. 锡林河流域厚壁菌群和拟杆菌群的生物地理学研究. 呼和浩特:内蒙古大学, 2021.
[30] 周界, 李明, 徐友阳, 等. 基于高通量测序的穿心莲连作根际土壤细菌群落多样性分析. 华南农业大学学报, 2021, 42(3):55-63.
[31] 张重义, 陈慧, 杨艳会, 等. 连作对地黄根际土壤细菌群落多样性的影响. 应用生态学报, 2010, 21(11):2843-2848.
[32] 周慕邱, 戴彬, 何轶, 等. 烤烟间作对烟株根际土壤酶活性和细菌群落的影响. 分子植物育种. (2023-08-28)[2023-10-23]. https://link.cnki.net/urlid/46.1068.S.20230825.1436.004.
[33] 田程, 邱婷, 朱菲莹, 等. 氧化钙对西瓜枯萎病及根际细菌群落的调控. 湖南农业大学学报(自然科学版), 2018, 44(6):620-624.
[34] 向立刚, 周浩, 汪汉成, 等. 健康与感染青枯病烟株根际土壤与茎秆细菌群落结构与多样性. 微生物学报, 2019, 59(10):1984-1999.
[35] 郭子雯, 陈婧, 焦莉苹, 等. 大豆胞囊线虫胁迫下不同抗性红小豆根际土壤细菌群落多样性分析. 西南农业学报, 2023, 36(8):1711-1717.
[36] Wang X B, Song D L, Liang G Q, et al. Maize biochar addition rate influences soil enzyme activity and microbial community composition in a fluvo-aquic soil. Applied Soil Ecology, 2015,96:265-272.
[37] Diamantidis G, Effosse A, Potier P, et al. Purification and characterization of the first bacterial laccase in the rhizospheric bacterium Azospirillum lipoferum. Soil Biology and Biochemistry, 2000, 32(7):919-927.
[1] Ren Yongfu, Li Jiayi, Chen Guopeng, Pu Tian, Chen Hong, Wang Xiaochun. Effects of Different Planting Patterns on the Yield and Efficiency of Maize in Strip Intercropping System [J]. Crops, 2025, 41(2): 101-108.
[2] Tian Wenqiang, Wang Hongyi, Nie Lingfan, Sun Ganggang, Zhang Jun, Zhang Qiangbin, Yu Shan, Li Jiahao, Zhang Jinshan, Shi Shubing. The Effects of Sowing Date and Sowing Rate on the Growth, Dry Matter Accumulation and Yield of Extremely Late-Sown Wheat Population [J]. Crops, 2025, 41(2): 115-122.
[3] Zhao Lingling, Li Guifang, Cheng Chu, Zheng Mingjie, Hu Min, Zhu Jianfeng, Shen Ayi, Shen Aga, Wang Junzhen, Shao Meihong. Preliminary Report on Introduction Experiment of New Buckwheat Varieties in Zhejiang Province [J]. Crops, 2025, 41(2): 86-92.
[4] Ma Yingchen, Wang Jiatong, Feng Yanfei, Ma Haoxiong, Ren Xuejun, Guo Zhenqing, Li Yun, Han Yucui, Lin Xiaohu. Impacts of the Residual Effects of the Combined Application of Compound Fertilizers and Microbial Inoculant on Soil Physicochemical Properties and Quality of Foxtail Millet [J]. Crops, 2025, 41(2): 141-148.
[5] Ji Jinghong, Liu Shuangquan, Ma Xingzhu, Hao Xiaoyu, Zheng Yu, Zhao Yue, Wang Xiaojun, Kuang Enjun. Effects of Different Controlled-Release Urea on Agronomic Traits, Yield and Nitrogen Use Efficiency of Cold Region Rice [J]. Crops, 2025, 41(2): 149-154.
[6] Jin Dandan, Sui Shijiang, Chen Yue, Li Bo, Qu Hang, Gong Liang. Effects of Straw Returning with Nitrogen Application Reduction on Yield and Nitrogen Utilization of Rice in Liaohe Plain [J]. Crops, 2025, 41(2): 172-179.
[7] Zhang Lina, Yang Wenping, Su Miao, Zhang Zhixuan, Li Junhui, Aamir Ali, Chen Jie, Gao Zhiqiang, Yang Zhenping. Effect of Foliar Application of Selenium onNutritional Quality of Oat Grain [J]. Crops, 2025, 41(2): 196-206.
[8] Li Yunxia, Yang Jiashuo, Li Yangyang, Xiang Shipeng, Yu Jinlong, Li Bin, Zheng Weiwei, Liu Lu. Effects of Different Transplanting Periods on the Growth, Development and Yield Quality of Flue-Cured Tobacco in Tobacco-Rice Rotation Area [J]. Crops, 2025, 41(2): 222-227.
[9] Li Junzhi, Dou Shuang, Wang Xiaodong, Zhang Meng, Xiao Jibing. Effects of Different Intercropping Patterns on Sorghum Growth and Development [J]. Crops, 2025, 41(2): 234-240.
[10] Luo Jianke, Zhang Kehou, Wang Zeyu, Zhang Pingzhen, Nan Ming. Research on the Production Performance of 18 Oat Varieties (Lines) in the Irrigation Area along the Yellow River in Baiyin City [J]. Crops, 2025, 41(2): 93-100.
[11] Zhang Jiazhi, Zhao Yuhan, Ding Junjie, Yao Liangliang, Qiu Lei, Zhang Maoming, Wang Zijie, Gao Xuedong, Huang Chengliang, Cui Shize, Yang Xiaohe. Effects of “Double-Exemption Dense Seedling” Technique on Seedling Quality and Enzyme Activity of Rice in Cold Region [J]. Crops, 2025, 41(2): 109-114.
[12] Long Weihua, Xian Zhihui, Zhang Zheng, Alibieligen·Hazitai , Zulehumaer·Wusimanjiang , Pu Huiming, Hu Maolong. Adaptability Analysis of Non-Transgenic Herbicide-Resistant Hybrid Rapeseed Lines from Lower Reaches of the Yangtze River in Ili River Valley, Xinjiang [J]. Crops, 2025, 41(1): 111-116.
[13] Chu Zhaokang, Wang Shiji, Bi Jianjian, Zhang Lin, Peng Chen, Chen Xiang, Wu Wenming. Effects of Sowing Dates on Yield and Filling Characteristics of Summer Maize in the Central Yangtze-Huaihe Region [J]. Crops, 2025, 41(1): 117-122.
[14] Zhang Kaikai, Zhao Deming, Ma Juhua, Bai Pengjun, Ma Peng, Chen Hui, Xu Wenjie, Huang Caixia, Liu Zhongyu. Effects of Furrow Straw Mulching on Soil Hydrothermal Characteristics and Yield of Potato under Dry Cultivation [J]. Crops, 2025, 41(1): 139-146.
[15] Lü Shuli, Ding Fang, Zheng Dongfang. Response of Photosynthetic Characteristics, Yield and Quality of Sesame Capsule to Different Chemical Ripening Agents [J]. Crops, 2025, 41(1): 155-161.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!