Crops ›› 2025, Vol. 41 ›› Issue (6): 58-66.doi: 10.16035/j.issn.1001-7283.2025.06.008

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Response of Different Wax Gourd Varieties to Low-Salt Stress and Comprehensive Evaluation of Their Salt Tolerance

Chen Yi1,2(), Chen Xiao1(), Zhang Maoxing2, Li Jing1, Chang Jingjing1, Li Jiawei1, Lin Haiqing1, Chen Xingping3, Deng Xiaoliang3, Xie Dasen1, Guo Shaolong3, Shen Zhongdeng4, Zhang Baige1()   

  1. 1 Vegetable Research Institute, Guangdong Academy of Agricultural Sciences / Key Laboratory for New Technology Research of Vegetable, Guangzhou 510640, Guangdong, China
    2 Foshan University, Foshan 528000, Guangdong, China
    3 Guangdong Provincial Improved Variety Introduce Service Corporation, Guangzhou 510091, Guangdong, China
    4 Guangdong Lübao Agricultural Science and Technology Development Corporation, Shaoguan 512600, Guangdong, China
  • Received:2024-07-07 Revised:2024-10-16 Online:2025-12-15 Published:2025-12-12

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Abstract:

Exploring the responses to salt stress among wax gourd varieties and comprehensively evaluating their salt tolerance is of great significance for identifying salt-tolerant varieties, improving the production capacity of way gourd in saline soils. Seven wax gourd varieties were measured and comprehensively evaluated for salinity tolerance in the germination and seedling stages of related physiological indexes in a simulated stress environment with NaCl solution. The results showed that 100 mmol/L NaCl treatment was determined to be a suitable salt concentration for the identification of salt tolerance in wax gourd. There were significant differences in germination potential, germination rate, plant height, root length, photochemical quenching coefficient, electron transfer rate, superoxide dismutase activity, and peroxidase activity among wax gourd varieties under salt stress. Through principal component analysis and comprehensive evaluation value (D-value) calculation, it was found that ?Fenpidonggua? and ?Tiezhu 2? were more salt-tolerant, and ‘Yuxiang xiaodonggua’ was less salt-tolerant. Using correlation analysis, germination index, radicle length, plant height, root length, maximum quantum yield, and antioxidant enzyme activities were screened out as relevant indexes for evaluating the salt tolerance of wax gourd.

Key words: Wax gourd, Germination period, Seedling stage, Salt stress, Comprehensive evaluation

Fig.1

Effects of different NaCl concentration treatments on the germination of wax gourd seeds"

Fig.2

Differential response of seed germination to different NaCl concentrations among wax gourd varieties Different lowercase letters indicate significant differences at P < 0.05 level, the same below."

Fig.3

Effects of different NaCl treatments on the growth of wax gourd seedlings of different varieties after six days of treatment"

Fig.4

Effects of 100 and 150 mmol/L NaCl treatments on the growth of different varieties of wax gourd seedlings"

Fig.5

Effects of salt stress on the growth of different wax gourd varieties seedlings"

Fig.6

Effects of salt stress on root growth of different wax gourd varieties"

Fig.7

Effects of salt stress on chlorophyll fluorescence parameters of leaves of different wax gourd varieties"

Table 1

Effects of salt stress on antioxidant enzyme activities of wax gourd leaves"

处理
Treatment		 品种
Variety		 SOD
(U/g FW)		 POD
[U/(g·s) FW]		 CAT
[U/(g·s) FW]
CK MY 98.9±1.5g 24.5±3.5d 9.8±2.6efg
XQ 119.6±3.7efg 23.0±3.5d 10.3±1.5defg
YX 93.4±11.3g 23.0±2.7d 8.3±0.6fg
FP 100.6±15.0g 27.0±1.7d 13.0±2.0cdef
TZ 109.1±18.2fg 27.7±2.3d 12.7±1.5cdef
DB 101.3±3.7g 24.8±7.1d 13.8±1.0bcde
XY 99.3±16.8g 24.2±0.3d 7.5±1.3g
100 mmol/L
NaCl		 MY 149.7±18.5cd 66.2±11.9bc 14.0±3.5bcde
XQ 141.5±21.7cde 59.3±11.1c 14.7±1.0bcd
YX 116.6±15.6efg 55.3±12.5c 11.7±2.1defg
FP 287.0±1.7a 87.3±16.0a 23.3±4.6a
TZ 236.5±11.5b 86.0±2.7a 18.0±1.7b
DB 166.8±14.1c 79.0±5.3ab 22.8±1.3a
XY 131.0±20.8def 68.0±6.9bc 16.5±3.9bc

Table 2

Comprehensive evaluation of seed germination and seedling stages of wax gourd under salt stress"

品种Variety Xi1 Xi2 Xi3 μXi1 μXi2 μXi3 D
MY -0.42 -0.73 -1.52 0.3 0.23 0.00 0.25
XQ -0.82 -0.85 0.91 0.16 0.18 0.85 0.25
YX -1.27 0.18 -0.12 0.00 0.57 0.49 0.18
FP 1.58 0.33 0.00 1.00 0.63 0.54 0.87
TZ 0.97 -1.32 0.38 0.79 0.00 0.67 0.61
DB 0.16 1.29 -0.97 0.50 1.00 0.19 0.57
XY -0.20 1.10 1.32 0.38 0.93 1.00 0.56

Fig.8

Correlation analysis of salt tolerance in wax gourd D: D-value, GP: germination potential, GR: germination rate, Gl: germination index, RaL: radicle length, ADW: aboveground dry weight, UDW: underground dry weight, RCR: root-shoot ratio, PH: plant height, RL: root length, RSA: root surface area, RV: root volume, RTN: root tip number. “*”indicates significant correlation (P < 0.05),“**”indicates extremely significant correlation (P < 0.01),“***”indicates extremely significant correlation (P < 0.001)."

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