Crops ›› 2016, Vol. 32 ›› Issue (4): 36-40.doi: 10.16035/j.issn.1001-7283.2016.04.006

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Isozyme Analysis of a New Cytoplasmic Male Sterile Line 1193A and Its Maintain Line in Brassica napus

Yin Mingzhi1,2,Guan Chunyun1   

  1. 1 Hunan Agricultural University/Hunan Branch of National Oil Crops Improvement Center,Changsha 410128,Hunan,China
    2 Zunyi Normal University,Zunyi 563002,Guizhou,China
  • Received:2016-04-11 Revised:2016-06-14 Online:2016-08-15 Published:2018-08-26
  • Contact: Chunyun Guan

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Abstract:

In this study, the POD, ATP and EST isozymes from new cytoplasmic male sterile line 1193A and maintain line 1193B of Brassica napus L. in the development of flower buds were analyzed by polyacrylamide gel electrophoresis. The results showed that there were differences in the number of isozyme bands and expression between the male sterile line and the maintain line in the different stages of the growth and development of the flower buds. In later developmental stage,POD isozymes bands activity of the fastest mobility in the male sterile line 1193A was significantly stronger than that of the maintain line 1193B; ATP and EST isozyme activity of the male sterile line 1193A became weakened gradually with development of the flower buds, and had the lack of enzyme bands at the later stage of development.

Key words: Brassica napus, Cytoplasmic male sterile line, Maintain line, Isozyme

Fig.1

Polyacrylamide gel electrophoretogram of POD 1~3 indicate big buds,middle buds and small buds respectively in 1193A;4~ 6 indicate big buds,middle buds and small buds respectively in 1193B"

Fig.2

Polyacrylamide gel electrophoretogram of ATP 1~3 indicate small buds,middle buds and big buds respectively in 1193A;4~6 indicate small buds,middle buds and big buds respectively in 1193B,the same below"

Fig.3

Polyacrylamide gel electrophoretogram of EST"

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