Crops ›› 2018, Vol. 34 ›› Issue (5): 156-161.doi: 10.16035/j.issn.1001-7283.2018.05.025

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Effects of Sinorhizobium SD101 Inoculation and Shading on Nitrogen Fixation and Photosynthesis of Medicago sativa L.

Liu Shuxia1,2,Wei Guojiang2,Jing Ruiyong1,Wang Liyan1,Guan Xiangjun2,Sun Yufeng2,Wang Guangda1,Han Ruyue1,Shi Jie2,Guo Yongxia1   

  1. 1 Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
    2 Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163319, Heilongjiang, China
  • Received:2018-04-16 Revised:2018-07-13 Online:2018-10-15 Published:2018-10-12
  • Contact: Yongxia Guo

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Abstract:

Plant growth and photosynthetic indexes in alfalfa (Medicago sativa L. WL319) were investigated after inoculation with Sinorhizobium fredii SD101 under shaded and subsequent recovered conditions in order to study the interactive effects of shading and nitrogen supply on plant growth and photosynthetic physiology. The results showed that under non-shading conditions, plant biomass, chlorophyll contents and photosynthetic capacity all significantly increased by inoculating with Sinorhizobium fredii SD101 as compared to the control alfalfa. Under shading condition, photosynthetic rate of the inoculated alfalfa decreased compared to the non-inoculated control alfalfa due to the influences of stomatal and non-stomatal factors, which lead the inoculated rhizobia not to play the role in alfalfa nitrogen fixation. After the restoration of light, the photosynthetic parameters were not recovered to the degree as the non-inoculated control. It could be inferred that only under suitable illumination condition symbiotic nitrogen fixation between alfalfa and Sinorhizobium fredii SD101 could promote the plant growth and photosynthesis.

Key words: Alfalfa, Shading, Rhizobia, Photosynthetic parameters

Table 1

Effects of shading and rhizobia inoculation on growth and nitrogen capacity in alfalfa"

项目Item 未避光Unshaded 避光Shaded
未接种
Non-inoculated		 接种SD101
Inoculated SD101		 未接种
Non-inoculated		 接种SD101
Inoculated SD101
株高Plant height (cm) 76.54±0.12ab 80.47±0.09a 68.27±0.20b 78.39±0.15ab
地上生物量Aboveground biomass (g) 1.84±0.10b 2.03±0.14a 1.01±0.13c 1.03±0.09c
叶面积Leaf area (cm2) 1.17±0.05a 1.23±0.07a 0.76±0.01b 0.81±0.03b
单株根瘤数Nodules per plant 16.55±0.05b 20.11±0.02a 9.12±0.08c 10.43±0.05c
单个根瘤重Single nodule weight (g) 0.0043±0.0001b 0.0051±0.0004a 0.0011±0.0003c 0.0014±0.0000c
固氮酶活性Nitrogenase activity [ug/(g·h)] 30.3±0.3b 35.0±0.6a 18.7±0.5c 22.6±0.1c

Table 2

Effects of shading and rhizobia inoculation on alfalfa leaf chlorophyll contents ug/gFW"

项目Item 未避光Unshaded 避光Shaded
未接种
Non-inoculated		 接种SD101
Inoculated SD101		 未接种
Non-inoculated		 接种SD101
Inoculated SD101
Chl a 1 837.4±23.1c 2 103.1±37.6b 2 321.9±26.4ab 2 562.5±25.9a
Chl b 623.1±11.2c 1 210.0±12.5a 954.6±8.9b 882.7±10.8b
总叶绿素含量Total Chlorophyll content 2 460.5±35.2b 3 313.1±33.0a 3 276.5±29.5a 3 345.2±21.2a
Chl a/Chl b 2.95±0.12a 1.74±0.08b 2.43±0.09ab 2.90±1.04a

Table 3

Effects of shading and rhizobia inoculation on photosynthetic parameters in alfalfa leaves"

项目Item 未接种Non-inoculated 接种SD101 Inoculated SD101
未避光Unshaded 避光Shaded 未避光Unshaded 避光Shaded
Pn [umol/(m2·s)] 5.04±0.10b 4.16±0.08b 7.60±0.13b 2.92±0.11c
Gs [mmol/(m2·s)] 0.076±0.001b 0.120±0.002a 0.124±0.008a 0.025±0.016c
Ci (umol/mol) 233.05±2.12b 304.15±4.03a 259.77±4.35b 248.14±4.52b

Table 4

Alfalfa leaf photosynthetic parameters after light recovery"

项目Item 未接种Non-inoculated 接种SD101 Inoculated SD101
未避光Unshaded 避光Shaded 未避光Unshaded 避光Shaded
Pn [umol/(m2·s)] 4.38±0.21b 6.79±0.10a 4.67±0.09b 5.22±0.14b
Gs [mmol/(m2·s)] 0.072±0.005b 0.187±0.022a 0.089±0.010b 0.123±0.016b
Ci (umol/mol) 240.27±3.14b 289.48±2.58a 260.12±5.62ab 269.38±6.17ab

Table 5

Effects of shading and rhizobia inoculation on chlorophyll fluorescence parameters in alfalfa leaves"

项目Item 未接种Non-inoculated 接种SD101 Inoculated SD101
未避光Unshaded 避光Shaded 未避光Unshaded 避光Shaded
Fv/Fm 0.831±0.004ab 0.816±0.009b 0.841±0.006a 0.832±0.001ab
ΦPSⅡ 0.622±0.015a 0.486±0.012c 0.638±0.018a 0.574±0.023b
qP 0.806±0.017a 0.701±0.020c 0.810±0.015a 0.752±0.011b
qN 0.279±0.020b 0.501±0.011a 0.275±0.009b 0.321±0.016b

Table 6

Alfalfa leaf chlorophyll fluorescence parameters after light recovery"

项目Item 未接种Non-inoculated 接种SD101 Inoculated SD101
未避光Unshaded 避光Shaded 未避光Unshaded 避光Shaded
Fv/Fm 0.822±0.001c 0.846±0.004a 0.836±0.013b 0.843±0.014ab
ΦPSⅡ 0.635±0.011a 0.599±0.014b 0.638±0.006a 0.636±0.016a
qP 0.847±0.009a 0.810±0.011c 0.849±0.009a 0.828±0.003b
qN 0.304±0.016a 0.284±0.005a 0.289±0.008a 0.247±0.007b
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