Crops ›› 2020, Vol. 36 ›› Issue (6): 8-16.doi: 10.16035/j.issn.1001-7283.2020.06.002

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Proteomic Comparation Analysis of Thylakoid in Leaves of G-Locus Near Isogenic Line in Soybean

Zhao Yuyang1(), Song Jian2, Qiu Lijuan1()   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Germplasm & Biotechnology (Ministry of Agriculture and Rural Affairs), Beijing 100081, China
    2College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China
  • Received:2020-04-20 Revised:2020-10-26 Online:2020-12-15 Published:2020-12-09
  • Contact: Qiu Lijuan E-mail:398884889@qq.com;qiulijuan@caas.cn

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Abstract:

Thylakoid is the key carrier of photosynthesis and electron transport and also the core component of chloroplast, which is particularly important in the study of plant subcellular organelle proteome. In order to explore the proteome of thylakoid in soybean leaves, a pair of G-locus near-isogenic line (NIL-G and NIL-Y) with 97.6% similarity in the genetic background was used as materials. SDS-PAGE and mass spectrometry were used to isolate the thylakoid protein from soybean leaves. The results showed that the number of total protein and specific protein in NIL-G was higher than that in NIL-Y, respectively. The numbers of total protein and specific protein in NIL-G were 2 170 and 1 140, while 1 730 and 700 in NIL-Y. GO annotation and KEGG pathway analysis of these specific proteins showed that although the high similarity among genetic background, there are differences in the biological pathway, cell components, and molecular functions of thylakoid proteins in soybean. This provide a new clue to the future research on thylakoid proteins in soybean.

Key words: Soybean, Mass spectrometry, Thylakoid, Proteome

Fig.1

Detection of thylakoid proteins in leaves of near-isogenic lines by SDS-PAGE"

Table 1

Identification of thylakoid proteins number in soybean leaves of near-isogenic lines NIL-G and NIL-Y by segmented mass spectrometry"

材料
Material		 < 25kDa 25~45kDa > 45kDa 特异
Specific		 共有
Same		 总计
Total
NIL-G 485 656 1 029 1 140 1 030 2 170
NIL-Y 373 458 899 700 1 730

Fig.2

GO annotation of specific proteins of near-isogenic lines NIL-G and NIL-Y at biological pathway level"

Fig.3

GO annotation of specific proteins of near-isogenic lines NIL-G and NIL-Y at cellular component level"

Fig.4

GO annotation of specific proteins of near-isogenic lines NIL-G and NIL-Y at molecular functional level"

Fig.5

Analysis of KEGG pathway of specific proteins in near-isogenic line NIL-G"

Fig.6

Analysis of KEGG pathway of specific proteins in near-isogenic line NIL-Y"

Table 2

Specific proteins related to photosynthesis and antenna in near-isogenic lines NIL-G and NIL-Y"

蛋白通路
Pathway of protein		 NIL-G NIL-Y
基因编号Gene accession 功能Function 基因编号Gene accession 功能Function
光合作用 A5Z2K3 PSⅠ亚基PsaD C6SWI3 PSⅡR类亚基
Photosynthesis C6SX81 未知蛋白 C6SXP3 未知蛋白
C6SW43 未知蛋白 C6T308 假设的ATP合成酶亚单位b
C6T2Z2 未知蛋白 C6TFR5 未知蛋白
I1MNK0 放氧增强蛋白1 I1M3V1 PSⅡ修复蛋白PSB27-H1
I1M100 ATP合酶γ链
I1JCG8 铁氧还蛋白—NADP还原酶 -
I1NGD2 PSⅠ反应中心亚基II
A0A0R4J4B4 铁氧还蛋白—NADP还原酶
A0A0R4J389 PSⅠ反应中心亚基II
C6TC81 未知蛋白
I1JJ05 放氧增强蛋白2
I1K498 铁氧还蛋白-A
光合作用-天线蛋白 I1KR46 叶绿素a/b结合蛋白CP24 I1MNM3 叶绿素a/b结合蛋白6A
Photosynthesis- C6T8S8 未知蛋白 A0A0R0ERF9 叶绿素a/b结合蛋白CP26
antenna protein A0A0R0JQ91 叶绿素a/b结合蛋白P4
C6TM32 LHCII 1型叶绿素a/b结合蛋白
Q43437 PSⅡ的I型叶绿素a/b结合蛋白
A0A0R0K962 叶绿素a/b结合蛋白P4
I1MZ32 叶绿素a/b结合蛋白CP29

Fig.7

Hierarchical clustering analysis of proteins with different expression of near-isogenic lines NIL-G and NIL-Y"

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