Crops ›› 2019, Vol. 35 ›› Issue (3): 55-65.doi: 10.16035/j.issn.1001-7283.2019.03.010

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Genome-Wide Identification and Expression Patterns Analysis of the PHD Family Protein in Glycine max

Yang Junkai,Shen Yang,Cai Xiaoxi,Wu Shengyang,Li Jianwei,Sun Mingzhe,Jia Bowei,Sun Xiaoli   

  1. College of Agronomy, Heilongjiang Bayi Agricultural University/Crop Stress Molecular Biology Laboratory, Daqing 163319, Heilongjiang, China
  • Received:2018-10-30 Revised:2019-02-27 Online:2019-06-15 Published:2019-06-12
  • Contact: Xiaoli Sun

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Abstract:

PHD (Plant Homeodomain Finger) family genes encode a class of zinc finger transcription factor proteins, which are widely involved in regulating plant growth and stress responses. In this study, through genome-wide analysis, we identified a total of 95 PHD family proteins in soybean proteome. We further performed the synteny analysis, constructed the phylogenic tree, identified the gene structures and functional domains, analyzed the gene ontology (GO) annotation, and investigated the expression patterns in different tissues and under abiotic stresses. These data provided comprehensive information regarding the gene duplication, family evolution, conserved domains and gene expression of the soybean PHD family. Our results presented in this study suggest that soybean PHD family displays a great divergence in family evolution, gene structure and conserved domain organization; possibly participates in different molecular processes, including Zn 2+ binding, DNA binding and epigenetic regulation; and probably controls plant development and environmental stress responses. In summary, these findings will provide a crucial cue for further characterizing the biological function of soybean PHD family in plant growth and environmental stress responses.

Key words: Soybean, PHD transcription factor, Gene family analysis, Expression patterns, Environmental stress

Fig.1

Sequence alignment of the PHD domains within soybean PHD family proteins"

Fig.2

Chromosome location and gene duplication of soybean PHD family genes"

Fig.3

Phylogenetic relationship of the soybean PHD family genes"

Fig.4

Gene structure of the soybean PHD family genes"

Fig.5

Conserved domains of the soybean PHD family proteins"

Fig.6

Ontology mapping of the soybean PHD family genes"

Fig.7

Tissue specific expression of the soybean PHD family genes"

Fig.8

Expression profiles of soybean PHD family genes under different environmental stresses"

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