Crops ›› 2023, Vol. 39 ›› Issue (4): 202-209.doi: 10.16035/j.issn.1001-7283.2023.04.029

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Effects of Exogenous Melatonin on Growth and Yield of Adzuki Bean under Drought Stress at Seedling Stage

Jiang Shan(), Liu Jia, Cao Liang, Ren Chunyuan, Jin Xijun, Zhang Yuxian()   

  1. College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
  • Received:2022-02-22 Revised:2023-05-15 Online:2023-08-15 Published:2023-08-15

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Abstract:

Adzuki bean cultivar Pearl Red was used as the test material in pot under portable rainproof shed, and normal water supply as control (80% field water holding), melatonin treatment under drought stress (100μmol/L melatonin was sprayed on the day of water control beginning, soil water content was gradually regulated and maintained 50% field capacity), drought stress treatment (equal amount of water was sprayed on the day of water control beginning, soil water content was gradually regulated and maintained 50% field capacity), to study effects of melatonin on drought resistance, photosynthetic physiology, morphology and yield of adzuki bean under drought stress. The results showed that melatonin could significantly increase antioxidant enzyme activity and infiltration regulators, decrease reactive oxygen species (ROS) and malonaldehyde contents, and increase plant height, stem diameter, leaf area and dry matter accumulation under drought stress. Melatonin significantly increased the content of photosynthetic pigment, photosynthetic rate, chlorophyll fluorescence parameters and carbohydrates (sucrose, fructose, soluble sugar) in adzuki bean leaves under drought stress. The application of exogenous melatonin increased the number of pods and grains per plant by 2.18% and 4.88%, respectively, compared with drought stress. It was concluded that 100μmol/L melatonin spraying on leaf surface could improve the drought resistance of adzuki bean, reduce the inhibition of photosynthesis of adzuki bean by drought stress, promote the growth of adzuki bean seedling and increase yield.

Key words: Adzuki bean, Drought stress, Melatonin, Photosynthesis

Table 1

Effects of melatonin on growth of adzuki bean seedlings under drought stress"

喷施后天数
Days after spraying (d)		 处理
Treatment		 株高
Plant height (cm)		 茎粗
Stem diameter (mm)		 叶面积
Leaf area (mm2)		 地上干重
Shoot dry weight (g)		 地下干重
Root dry weight (g)
10 CK 13.07±0.38a 3.06±0.19a 6168.63±124.22a 0.86±0.04a 0.44±0.06a
D 10.93±0.41b 2.75±0.05a 4694.18±329.26a 0.75±0.03a 0.36±0.01a
D+M 11.90±0.45ab 2.90±0.07a 5706.56±401.30a 0.81±0.03a 0.40±0.04a
15 CK 15.83±1.03a 3.21±0.16a 7722.79±458.57a 1.55±0.08a 0.60±0.04a
D 12.80±0.18b 2.76±0.06a 5906.40±880.02b 0.93±0.10b 0.49±0.06b
D+M 13.77±1.29b 2.95±0.06a 6850.67±943.51ab 1.32±0.09ab 0.59±0.02ab
20 CK 20.30±0.44a 3.41±0.04b 8800.49±245.24a 2.67±0.06a 1.22±0.09a
D 15.97±0.18c 2.90±0.11ab 7092.89±598.23b 1.63±0.11b 0.85±0.05b
D+M 18.50±0.55b 3.01±0.14a 8070.84±120.23b 2.30±0.02ab 0.91±0.07b
25 CK 25.20±0.42a 3.50±0.02a 9156.13±593.47a 3.47±0.05a 1.76±0.11a
D 17.87±0.29c 3.05±0.04c 7789.10±962.75b 1.96±0.10b 0.96±0.10b
D+M 20.87±0.33b 3.26±0.06b 8922.64±612.18ab 2.58±0.13b 1.24±0.06b
30 CK 29.13±2.91a 3.78±0.13a 9329.80±519.87a 4.70±0.06a 2.07±0.11a
D 24.47±0.86b 3.20±0.18b 8038.34±485.77b 2.37±0.15b 1.21±0.07b
D+M 27.47±0.29a 3.58±0.14ab 9063.35±437.55ab 3.25±0.09b 1.38±0.11ab

Fig.1

Effects of melatonin on antioxidant enzyme activities under drought stress Different lowercase letters indicate significant difference at the P<0.05 level, the same below"

Fig.2

Effects of melatonin on membrane lipid peroxidation under drought stress"

Fig.3

Effects of melatonin on photosynthetic parameters of adzuki bean under drought stress"

Fig.4

Effects of melatonin on chlorophyll fluorescence parameters of adzuki bean under drought stress"

Fig.5

Effects of melatonin on photosynthetic pigment contents of adzuki bean under drought stress"

Fig.6

Effects of exogenous melatonin on sugar contents of adzuki bean under drought stress"

Table 2

Effects of melatonin on yield components under drought stress"

处理
Treatment		 单株荚数
Pod number
per plant		 单株粒数
Grain number
per plant		 百粒重
100-seed
weight (g)		 单株粒重
Grain weight
per plant (g)
CK 13.83±2.14a 48.00±3.74a 11.62±1.26a 5.28±1.42a
D 11.91±2.48ab 44.50±2.74b 11.06±0.12a 4.92±0.31a
D+M 12.17±1.17ab 46.67±3.14ab 11.10±0.18a 5.19±0.41a
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