Crops ›› 2023, Vol. 39 ›› Issue (6): 167-173.doi: 10.16035/j.issn.1001-7283.2023.06.023

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Effects of Different Fertilization Methods on Soil Bacterial Community Diversity in Soybean Rhizosphere

Ma Tao(), Xing Baolong, Zheng Minna   

  1. High Latitude Crops Institute, Shanxi Agricultural University, Datong 037008, Shanxi, China
  • Received:2022-05-30 Revised:2022-06-28 Online:2023-12-15 Published:2023-12-15

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Abstract:

The effects of five distinct fertilization treatments (T1, T2, T3, T4 and T5) on the diversity of the bacterial community and the nutritional status of the soil were investigated using Miseq high-throughput sequencing. The results showed that, the contents of alkali-hydrolyzed nitrogen (AN), available phosphorus (AP), available potassium (AK) and aboveground biomass were the highest with T4 treatment. Based on the three indicators of Shannon index, Ace index and Chao1 index, bacterial species were more abundant in the T2, T3, and T4 treatments. At the genus level, five treated rhizosphere soil bacteria were divided into 379 taxa. Redundancy analysis between relative soil bacterial richness and soil environmental factors showed that, AN, AP and AK showed significant positive correlation with Alterythrobacter, Pseudomonas and Pseudarthrobacter. Comprehensive analysis showed that in the compound fertilizer-organic fertilizer 30m3/ha (T4), the biological yield of Jindou 46 reached the maximum, its dominant flora was more conducive to the soil fertilizer cultivation and crop yield increase.

Key words: Fertilization method, Organic fertilizer, Inorganic fertilizer, Soil bacterial community

Table 1

Physical and chemical properties of soil and biomass with difference fertilization treatments"

处理
Treatment		 全氮
Total N (%)		 碱解氮
Available N (mg/kg)		 有效磷
Available P (mg/kg)		 速效钾
Available K (mg/kg)		 有机质
Soil organic matter (g/kg)		 pH 生物量
Biomass (g/m2)
T1 0.05±0.00a 41.43±3.65b 6.48±0.05b 80.33±7.69c 9.25±0.10b 8.81±0.05a 245.69b
T2 0.05±0.00a 35.50±2.99c 6.13±0.00b 110.33±10.01b 9.57±0.10a 8.80±0.05a 276.39ab
T3 0.06±0.00a 34.90±2.78c 6.78±0.00b 89.00±8.23c 9.87±0.20a 8.69±0.05a 270.34ab
T4 0.08±0.00a 68.73±6.32a 10.85±0.06a 201.33±17.95a 9.70±0.15ab 8.69±0.06a 301.11a
T5 0.06±0.01a 52.83±3.87ab 10.43±0.06a 174.33±11.33ab 10.28±0.20a 8.59±0.05b 275.36ab

Table 2

Bacterial sequencing and community α diversity index of soil with different fertilization treatments"

处理Treatment 序列数Raw read OTU 覆盖度Cover degree Shannon Ace Chao1
T1 68248 625±32.12b 0.9990±0.00a 3.99±0.23c 1567.98±23.65c 686.46±10.36b
T2 67527 847±40.13a 0.9998±0.00a 5.37±0.29a 1810.09±32.97a 735.61±15.29ab
T3 67042 795±38.65ab 0.9998±0.01a 5.47±0.34a 1856.45±37.45a 748.75±16.89ab
T4 67821 717±30.22b 0.9998±0.01a 5.48±0.33a 1875.14±35.26a 798.40±17.01a
T5 65994 619±18.49b 0.9960±0.00a 4.84±1.98b 1702.11±24.88b 729.18±16.77ab

Fig.1

Composition and structure of bacterial phyla (a) and genus (b) with different fertilization treatments"

Fig.2

UPGMA plot of the soil bacterial communities with different fertilization treatments"

Fig.3

PCA analysis plot of soil bacterial communities with different fertilization treatments"

Fig.4

Heatmap analysis of the bacterial community at the genus level with different treatments A, B, C, D and E represent T1, T2, T3, T4 and T5 treatments, respectively"

Fig.5

RDA analysis of soil bacteria relative abundance and among major environmental factors with different fertilization treatments at the genus level"

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