Crops ›› 2024, Vol. 40 ›› Issue (5): 140-145.doi: 10.16035/j.issn.1001-7283.2024.05.020

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Effects of Low Temperature Acclimation Duration on Cold Tolerance Physiological Characteristics in Vegetable Sweet Potato Seedlings

Xiao Xiao(), Zhong Kunquan, Tu Xiaoju, Yi Zhenxie()   

  1. College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, China
  • Received:2023-06-06 Revised:2023-10-27 Online:2024-10-15 Published:2024-10-16

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Abstract:

Using Fucaishu 18, a vegetable sweet potato variety, as the material, it was treated for 24, 48, and 72 hours at 15 °C and 12 °C, respectively, before applying a low-temperature stress treatment at 8 °C/5 °C. The physiological characteristics of antioxidant enzymes, osmotic mediators, membrane lipid peroxidation, and cold resistance were determined after stress 0, 24, 48, and 72 h after stress, as well as the restoration of room temperature 48, 48, and 48 h again low-temperature stress. These data were used to examine the effects of various low-temperature acclimation times on the cold tolerance of sweet potatoes. The results showed that with the extension of the low-temperature stress time, the SOD and POD activities of the low-temperature acclimation leaves gradually increased. Although the contents of soluble protein and soluble sugar fluctuated, the change range was relatively smoother than CK. During the low-temperature stress period, the MDA content of each low-temperature acclimation treatment was gradually decreased, and CK gradually increased with the extension of the stressed time. It was observed that there was a significant interaction between low-temperature acclimation and low-temperature stress, and that the two factors jointly induced an improvement in cold resistance. The analysis showed that there was a significant interaction between low temperature exercise and low temperature stress, and cold tolerance jointly induced the improvement of cold tolerance under the piecewise interaction of the two factors.

Key words: Vegetable sweet potato, Low-temperature acclimation, Cold tolerance, Physiological characteristics

Fig.1

Trend of SOD activity in different low-temperature acclimation treatments under low-temperature stress SY 72 h: normal temperature adaptation 72 h, XP 0 h: low temperature stress 0 h, XP 24 h: low temperature stress 24 h, XP 48 h: low temperature stress 48 h, XP 72 h: low temperature stress 72 h; Lowercase letters indicate significant differences in the same processing in different periods (P < 0.05); uppercase letters indicate significant differences between different processing in the same period (P < 0.05), the same below."

Fig.2

Comparison of SOD activity in various treatments after restoring normal temperature and re-low temperature stress"

Fig.3

Trends in POD activity in different low-temperature acclimation under low temperature stress"

Fig.4

Comparison of POD activity in each treatment after restoring normal temperature and re-low temperature stress"

Fig.5

Changes in the content of soluble proteins in different low-temperature acclimation under low temperature stress"

Fig.6

Comparison of the contents of soluble protein after restoring normal temperature and re-low temperature stress"

Fig.7

The trend of soluble sugar content in different low-temperature acclimation treatments under low-temperature stress"

Fig.8

Comparison of soluble sugar content in each treatment after restoring normal temperature and re-low temperature stress"

Fig.9

The trend of changing of MDA content in different low-temperature acclimation treatment under low-temperature stress"

Fig.10

Comparison of MDA content after restoring normal temperature and re-low temperature stress"

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