Crops ›› 2024, Vol. 40 ›› Issue (6): 218-225.doi: 10.16035/j.issn.1001-7283.2024.06.029

Previous Articles     Next Articles

Analysis of Growth, Development and Carbon Metabolism of Rice BR Receptor Mutant Fn189 at Jointing Stage

Hu Yaqing1,2(), Li Chunqing2, Wang Guan1, Xu Jiang2()   

  1. 1College of Agronomy, Jilin Agricultural University, Changchun 130118, Jilin, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture and Rural Affairs, Beijing 100081, China
  • Received:2024-03-10 Revised:2024-04-24 Online:2024-12-15 Published:2024-12-05

RichHTML

2

PDF (PC)

195

Abstract:

Rice brassinosteroids (BR) receptor BRI1 coding gene (OsBRI1) mutant Fn189 and its wild type Tz65 were used as test materials. Under field conditions, a randomized block design was used to study the effects of BR on growth and development and carbon metabolism-related physiological indexes of rice at jointing stage. The results showed that compared with the wild-type Tz65, the growth of Fn189 was inhibited at jointing stage, and the plant height and tiller number of Fn189 were decreased by 25.07% and 15.82%, respectively. In addition, the Rubisco activity of Fn189 leaves decreased at jointing stage, and the ability of leaves to fix CO2 decreased. Compared with the wild-type, the activities of sucrose phosphate synthetase, sucrose synthase, ADP-glucose pyrophosphorylase, soluble starch synthase and granule-bound starch synthase of Fn189 leaves decreased at jointing stage, and the activity of acid invertase increased, indicating that the synthesis of sucrose and starch in the mesophyll cells of Fn189 decreased, while the decomposition of sucrose accelerated. The results showed that the contents of sucrose, soluble sugar, starch and non-structural carbohydrates in Fn189 leaves were significantly decreased by 23.76%, 13.46%, 9.02% and 10.40%, respectively, compared with the wild-type. Finally, the yield of Fn189 was 71.04% lower than that of wild-type. Therefore, BR signal transduction not only affected the growth and development of rice at jointing stage, it also affected the photosynthesis of rice leaves and the metabolism of sucrose and starch, and ultimately affected the yield of rice.

Key words: Rice, Brassinosteroids, Mutant Fn189, Jointing stage, Growth and development, Carbon metabolism

Fig.1

Comparison of morphological indexes between Fn189 and Tz65 at jointing stage “*”and“**”indicate significant difference at P < 0.05 and P < 0.01 levels, respectively, the same below."

Fig.2

Comparison of photosynthetic indexes between Fn189 and Tz65 at jointing stage"

Fig.3

Comparison of sucrose and starch metabolism between Fn189 and Tz65 at jointing stage"

Table 1

Comparison of yield and its components of Fn189 and Tz65 at harvest stage"

材料
Material		 有效穗数
Effective number of panicles (/m2)		 穗粒数
Grains per panicle		 千粒重
1000-grain weight (g)		 结实率
Seed-setting rate (%)		 产量
Yield (kg/hm2)
Tz65 504.00 96.73 26.56 81.56 9818.80
Fn189 381.00** 106.40* 16.97** 42.58** 2843.27**
[1] 申春芳. 水稻栽培技术对稻米品质的影响. 世界热带农业信息, 2022(5):84-86.
[2] 刘奇华, 蔡建, 李天. 水稻籽粒中的淀粉合成关键酶及其与籽粒灌浆和稻米品质的关系. 植物生理学通讯, 2006, 42(6):1211-1216.
[3] 张国伟, 李凯, 李思嘉, 等. 减库对大豆叶片碳代谢的影响. 作物学报, 2022, 48(2):529-537.
doi: 10.3724/SP.J.1006.2022.14024
[4] Xiong M, Yu J W, Wang J D, et al. Brassinosteroids regulate rice seed germination through the BZR1-RAmy3D transcriptional module. Plant Physiology, 2022, 189(1):402-418.
doi: 10.1093/plphys/kiac043 pmid: 35139229
[5] Tong H N, Chu C C. Functional specificities of brassinosteroid and potential utilization for crop improvement. Trends in Plant Science, 2018, 23(11):1016-1028.
doi: S1360-1385(18)30187-0 pmid: 30220494
[6] 郭彤, 李亚静. 油菜素内酯影响小麦种子萌发的生理机制. 广东蚕业, 2021, 55(8):23-24.
[7] 王士银, 陈冉冉, 石庆华, 等. 开花期外施表油菜素内酯(epi-BR)对水稻的影响. 作物杂志, 2012(4):83-86.
[8] Thussagunpanit J, Jutamanee K, Sonjaroon W, et al. Effects of brassinosteroid and brassinosteroid mimic on photosynthetic efficiency and rice yield under heat stress. Photosynthetica, 2015, 53(2):312-320.
[9] 宁金花, 陆魁东, 霍治国, 等. 拔节期淹涝胁迫对水稻形态和产量构成因素的影响. 生态学杂志, 2014, 33(7):1818-1825.
[10] 程建峰, 戴廷波, 蒋海燕, 等. 水稻拔节期叶片碳氮代谢基因型差异及与氮素利用效率的关系. 中国水稻科学, 2012, 26 (1):101-108.
[11] 李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000.
[12] 张志良, 瞿伟菁. 植物生理学实验指导. 3版. 北京: 高等教育出版社, 2003.
[13] Choix F J, Bashan Y, Mendoza A, et al. Enhanced activity of ADP glucose pyrophosphorylase and formation of starch induced by Azospirillum brasilense in Chlorella vulgaris. Journal of Biotechnology, 2014, 177:22-34.
[14] Jiang H W, Dian W M, Wu P. Effect of high temperature on fine structure of amylopectin in rice endosperm by reducing the activity of the starch branching enzyme. Phytochemistry, 2003, 63(1):53-59.
pmid: 12657298
[15] Pan J, Lin S, Woodbury N W. Bacteriochlorophyll excited-state quenching pathways in bacterial reaction centers with the primary donor oxidized. The Journal of Physical Chemistry B, 2012, 116 (6):2014-2022.
[16] Makino A. Photosynthesis, grain yield, and nitrogen utilization in rice and wheat. Plant Physiology, 2011, 155(1):125-129.
doi: 10.1104/pp.110.165076 pmid: 20959423
[17] Sweetlove L J, Müller-Röber B, Willmitzer L, et al. The contribution of adenosine 5′-diphosphoglucose pyrophosphorylase to the control of starch synthesis in potato tubers. Planta, 1999, 209(3):330-337.
doi: 10.1007/s004250050640 pmid: 10502100
[18] 唐煜杰, 苗欢, 冯茜, 等. 合理施磷量提高水稻拔节期对大气增温的抵御能力. 植物营养与肥料学报, 2023, 29(8):1461- 1470.
[19] Coll Y, Coll F, Amorós A, et al. Brassinosteroids roles and applications: an up-date. Biológia, 2015, 70(6):726-732.
[20] Castorina G, Consonni G. The role of brassinosteroids in controlling plant height in poaceae: a genetic perspective. International Journal of Molecular Sciences, 2020, 21(4):1191.
[21] Tong H N, Xiao Y H, Liu D P, et al. Brassinosteroid regulates cell elongation by modulating gibberellin metabolism in rice. The Plant Cell, 2014, 26(11):4376-4393.
doi: 10.1105/tpc.114.132092 pmid: 25371548
[22] Tian P, Liu J F, Yan B H, et al. BRASSINOSTEROID- SIGNALING KINASE1-1, a positive regulator of brassinosteroid signalling, modulates plant architecture and grain size in rice. Journal of Experimental Botany, 2023, 74(1):238-295.
[23] Zhiponova M K, Vanhoutte I, Véronique B, et al. Brassinosteroid production and signaling differentially control cell division and expansion in the leaf. New Phytologist, 2013, 197(2):490-502.
doi: 10.1111/nph.12036 pmid: 23253334
[24] Tong H N, Liu L C, Jin Y, et al. DWARF and LOW-TILLERING acts as a direct downstream target of a GSK3/SHAGGY-like kinase to mediate brassinosteroid responses in rice. The Plant Cell, 2012, 24(6):2562-2577.
doi: 10.1105/tpc.112.097394 pmid: 22685166
[25] Fujikawa Y, Sakurai N, Sendo S, et al. Sugar metabolism in expanding husk leaves of flint corn (Zea mays L.) genotypes differing in husk leaf size. Journal of Agricultural Science, 2002, 139(1):37-45.
[26] Salas F, Maria G, Philip W, et al. From dwarves to giants? Plant height manipulation for biomass yield. Trends in Plant Science, 2009, 14(8):454-461.
doi: 10.1016/j.tplants.2009.06.005 pmid: 19616467
[27] Wu C Y, Trieu A, Radhakrishnan P, et al. Brassinosteroids regulate grain filling in rice. The Plant Cell, 2008, 20(8):2130- 2145.
[28] Xia X J, Huang L F, Zhou Y H, et al. Brassinosteroids promote photosynthesis and growth by enhancing activation of Rubisco and expression of photosynthetic genes in Cucumis sativus. Planta, 2009, 230(6):1185-1196.
[29] 何迷, 李小波, 黄静, 等. 水稻叶面积指数与产量关系研究进展. 农学学报, 2022, 12(8):1-5.
doi: 10.11923/j.issn.2095-4050.cjas2020-0269
[30] 尹林芝, 向焱赟, 伍湘, 等. 不同氮素水平下超级稻叶面积指数及产量变化特征. 分子植物育种. (2022-03-23) [2024-03-10]. http://kns.cnki.net/kcms/detail/46.1068.s.20220322.1718.008.html.
[31] Sammar A M R, Arif M I, Allah D, et al. Exploring the recuperative potential of brassinosteroids and nano-biochar on growth, physiology, and yield of wheat under drought stress. Scientific Reports, 2023, 13(1):15015.
[32] Yuan L Y, Shu S, Sun J, et al. Effects of 24-epibrassinolide on the photosynthetic characteristics, antioxidant system, and chloroplast ultrastructure in Cucumis sativus L. under Ca(NO3)2 stress. Photosynthesis Research, 2012, 112(3):205-214.
[33] 董登峰, 李杨瑞, 江立庚. 油菜素内酯对铝胁迫大豆光合特性的影响. 作物学报, 2008, 34(9):1673-1678.
[34] Jutamanee K, Sonjaroon W, Pankean P, et al. Effects of brassinosteroid and brassinosteroid mimic on photosynthetic efficiency and rice yield under heat stress. Photosynthetica, 2015, 53(2):312-320.
[35] Li X J, Guo X, Zhou Y H, et al. Overexpression of a brassinosteroid biosynthetic gene Dwarf enhances photosynthetic capacity through activation of Calvin cycle enzymes in tomato. BMC Plant Biology, 2016, 16(27):33.
[36] Gao Y, Jiang T, Xiang Y, et al. Epi-brassinolide positively affects chlorophyll content and dark-reaction enzymes of maize seedlings. Phyton (Buenos Aires), 2021, 90(5):1465-1476.
[37] 李赞堂, 王士银, 姜雯宇, 等. 穗分化期外施24-表油菜素内酯(EBR)促进水稻源、库及籽粒灌浆的生理机制. 作物学报, 2018, 44(4):581-590.
[38] 陈燕华, 王亚梁, 朱德峰, 等. 外源油菜素内酯缓解水稻穗分化期高温伤害的机理研究. 中国水稻科学, 2019, 33(5):457- 466.
doi: 10.16819/j.1001-7216.2019.9036
[39] 藏金萍, 赵艾佳, 赵亚林, 等. 油菜素内酯对玉米叶片捕光、CO2固定及有机物运输的影响. 中国农业科学, 2017, 50(21):4228-4234.
doi: 10.3864/j.issn.0578-1752.2017.21.017
[40] 刘海英, 郭天财, 朱云集, 等. 开花期外施表油菜素内酯(epi-BR)对小麦籽粒淀粉积累及其关键酶活性的影响. 作物学报, 2006, 26(1):924-930.
[1] Fa Xiaotong, Meng Qinghao, Wang Chen, Gu Hanzhu, Jing Wenjiang, Zhang Hao. Research Progress on Response of Rice Root Morphology and Physiology to Alternate Wetting and Drying Irrigation [J]. Crops, 2024, 40(6): 1-8.
[2] Li Fei, Bian Shaofeng, Xu Chen, Zhao Hongxiang, Song Hanglin, Wang Fuchen, Zhuang Yan. Effects of Ridge Side Cultivation on Maize Physiological Characteristics, Growth and Development in Sloping Farmland [J]. Crops, 2024, 40(6): 120-125.
[3] Wang Benfu, Yu Zhenyuan, Song Pingyuan, Zhang Zuolin, Zhang Zhisheng, Li Yang, Su Zhangfeng, Zheng Zhongchun, Cheng Jianping. Effects of Soil Amendments on Soil Characteristics and Rice Growth in Cold Waterlogged Paddy Field [J]. Crops, 2024, 40(6): 126-131.
[4] Zeng Qianqian, Zhang Zhenyuan, Ma Xiue, Fang Yinghan, Zhai Jinlei, Jin Tao, Liu Dong, Liu Zhangyong. Effects of Diatomite Application on Yield and Nitrogen Use Efficiency of Rice [J]. Crops, 2024, 40(6): 147-152.
[5] Sun Mingmao, Liu Lixia, Sun Hu, Cui Di. Analysis of Anthocyanin and Important Agronomic Traits in a Population of Recombinant Inbred Lines of Rice [J]. Crops, 2024, 40(6): 26-38.
[6] Yang Tiexin, Dong Liqiang, Ma Liang, Feng Yingying, Li Zhiqiang. Evaluation on Yield and Quality of Fragrant Japonica Rice in Plain Rice Production Region in Central Liaoning [J]. Crops, 2024, 40(6): 71-77.
[7] Sun Jiameng, Gao Yuan, Chen Hu, Hua Qin, Lin Quanxiang, Chen Qingquan, Li Jincai, Zhang Haitao. Phenotypic Analysis and Gene Mapping of Rice Mutant Chalkiness and Shrunken Endosperm-2 [J]. Crops, 2024, 40(5): 1-7.
[8] Tian Qinqin, Zhuo Le, Chen Nana, Zheng Dechao, Wu Xiaojing, Yu Peng, Chen Pingping, Yi Zhenxie. Effects of Calcium-Magnesium Hydrotalcite on Cadmium Content in Brown Rice of Double-Cropping Rice and Soil Characteristics [J]. Crops, 2024, 40(5): 131-139.
[9] Li Hongliang, Sun Yuyou, Wei Caiqiang, Liu Dan, Xie Zhong, Cheng Dujuan, Qu Jinling, Song Ze, Meng Xianghai, Zhao Yuntong, Shi Xinrui. Effects of Controlled Irrigation and Fertilization on Growth, Yield and Quality of Japonica Rice in Cold Region [J]. Crops, 2024, 40(5): 152-158.
[10] Liu Zichen, Shang Liyan, Ye Jiayu, Sheng Tian, Li Ruijie, Deng Jun, Tian Xiaohai, Zhang Yunbo, Huang Liying. Effects of Dense Planting with Reduced Nitrogen Input Cultivation on the Grain Quality of Hybrid Indica Rice [J]. Crops, 2024, 40(5): 194-203.
[11] Zhang Lili, Li Zhenyu, Chen Guanghong, Wang Shaolin, Xia Ming, Zheng Yingjie, Wang Ying, Wang Tong, Mao Ting, Yu Yahui. Analysis and Evaluation of Nutrient Composition of Special Rice Germplasm Resources Based on the Principal Component Analysis [J]. Crops, 2024, 40(5): 40-47.
[12] He Jiahui, Li Yanfeng, Yan Tianze, Zhang Xuanwen, Qin Peng, Guo Jinyou, Wang Kai, Liu Xionglun, Yang Yuanzhu. The Effects of Reducing Nitrogen Fertilizer Application on the Yield and Quality of Super Rice Weiliangyou 8612 [J]. Crops, 2024, 40(5): 73-79.
[13] Ou Yingzhuo, Zhao Qing, Gu Huaiying, Zhou Yuyang, Liu Changhua, Meng Lijun. Application Status of Chlorate in Nitrate Nitrogen Research of Rice [J]. Crops, 2024, 40(4): 1-7.
[14] Yuan Shuai, He Mingjuan, Cui Can, Han Yu, Yu Peng, Yi Zhenxie. Effects of Different Base Application Amounts of Calcium- Magnesium Hydrotalcite in Early Rice on Yield and Rice Quality of Double-Cropping Rice in Southern Hunan [J]. Crops, 2024, 40(4): 113-120.
[15] Zhou Zhou, Shen Xinya, Wang Jun, Liu Lijun. Effects of Combination of Controlled-Release Fertilizer and Common Urea on Yield, Nitrogen Use Efficiency and Grain Quality in Rice [J]. Crops, 2024, 40(4): 180-187.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!