Crops ›› 2025, Vol. 41 ›› Issue (6): 203-209.doi: 10.16035/j.issn.1001-7283.2025.06.025

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Effect of Immune Inducer ZNC on Seedling Growth and Physiological Response of Oat under Saline-Alkali Stress

Guo Longyu(), Gao Xinmei(), Fu Ying, Zhang Mingwei, Wang Ying, Li Wurijimusi, Wurilige , Wang Yingjie, Wang Jingyu, Yang Fengting, Gu Yanru, Quan Yu   

  1. Hinggan League Institute of Agricultural and Husbandry Sciences, Ulanhot 137400, Inner Mongolia, China
  • Received:2024-07-26 Revised:2024-09-07 Online:2025-12-15 Published:2025-12-12

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Abstract:

Exploring the effects of immune inducer ZNC on the growth, development, and saline-alkali tolerance of oat seedlings, providing theoretical basis for the study of oat saline-alkali tolerance and green, high-yield, and high-efficiency cultivation. This study used Baiyan 2 as the experimental material and explored the effects of foliar spraying of different concentrations of ZNC on growth characteristics such as plant height, stem diameter, total biomass, and physiological indicators such as leaf antioxidant enzyme activity, malondialdehyde, and osmotic regulator content under saline-alkali stress conditions of 200 mmol/L (NaCl:NaHCO3=1:1) through pot experiments. The results showed that compared with the control group under saline-alkali stress, spraying 50 μg/L ZNC on oat leaves significantly increased seedling height, stem diameter, main root length, fresh weight of shoot and root by 16.85%, 40.70%, 20.02%, 53.21%, and 56.10%, respectively; the activities of superoxide dismutase, peroxidase, and dehydrogenase in seedling leaves were significantly increased by 4.99%, 35.86%, and 102.66%, respectively; the malondialdehyde content significantly decreased by 28.65%; the contents of soluble protein, soluble sugar, and proline significantly increased by 51.71%, 26.69%, and 154.51%, respectively. It was shown that spraying suitable concentrations (50.0 μg/L) of ZNC on oat leaves can promote seedling growth, reduce cell membrane oxidative damage, enhance osmotic regulation ability, improve saline-alkali tolerance of oat seedlings, and effectively alleviate the damage caused by mixed saline-alkali stress to oat seedling growth.

Key words: Oat, Saline-alkali stress, Immune inducer ZNC, Growth and development, Physiological adaptation

Table 1

Experimental design"

处理
Treatment		 ZNC浓度
Concentration of
ZNC (μg/L)		 盐碱溶液浓度
Concentration of saline-
alkali (mmol/L)
CK1 0.0 0.0
CK2 0.0 200.0
T1 12.5 200.0
T2 25.0 200.0
T3 50.0 200.0
T4 100.0 200.0
T5 200.0 200.0
T6 400.0 200.0

Table 2

Effect of foliar spraying of ZNC on growth of oat seedlings under saline-alkali stress"

处理
Treatment		 株高
Plant height (cm)		 茎粗
Stem diameter (mm)		 主根长
Main root length (cm)		 地上部鲜重
Fresh weight of shoot (g)		 根鲜重
Fresh weight of root (g)		 总生物量
Total biomass (g)
CK1 39.20±0.40b 2.49±0.32a 11.68±0.70ab 1.78±0.29a 0.51±0.06bc 2.29±0.35a
CK2 37.20±1.04c 1.72±0.09d 10.34±0.99b 1.09±0.22c 0.41±0.06c 1.50±0.26c
T1 38.20±0.35bc 1.91±0.10cd 11.48±1.16ab 1.19±0.29bc 0.53±0.10abc 1.72±0.20bc
T2 42.60±1.04a 2.13±0.13bc 11.88±1.62ab 1.53±0.35ab 0.58±0.10ab 2.11±0.28ab
T3 43.47±1.45a 2.42±0.16ab 12.41±0.67a 1.67±0.16a 0.64±0.08a 2.31±0.11a
T4 41.60±1.44a 2.23±0.31abc 11.39±0.93ab 1.38±0.16abc 0.54±0.07ab 1.92±0.23abc
T5 38.93±0.99bc 2.37±0.05ab 12.23±0.95a 1.40±0.09abc 0.55±0.02ab 1.96±0.09ab
T6 38.60±1.31bc 2.22±0.25abc 11.92±0.47ab 1.21±0.27bc 0.46±0.03bc 1.67±0.27bc

Fig.1

Effects of ZNC on antioxidant enzyme activities of oat seedling leaves under saline-alkali stress Different lowercase letters indicate significant differences at the P < 0.05 level. The same below."

Fig.2

Effects of ZNC on the content of MDA in oat seedling leaves under saline-alkali stress"

Fig.3

Effects of ZNC on osmotic regulatory substances in oat seedling leaves under saline-alkali stress"

Fig.4

Correlation between growth and physiological indexes of oat seedling PH: plant height; SD: stem diameter; MRL: main root length; TB: total biomass.“*”represents significant correlation (P < 0.05),“**”represents extremely significant correlation (P < 0.01)."

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