Crops ›› 2018, Vol. 34 ›› Issue (3): 68-76.doi: 10.16035/j.issn.1001-7283.2018.03.011

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Physiological and Genetic Analysis of Rice Mutant osnad1 Defective in Nitrogen Absorption

Zhang Li1,Li Zantang1,Wang Shiyin1,Ma Yanchao2,Dongfang Yang2,Li Xueyong1,Xu Jiang1   

  1. 1 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2 Hebei Normal University of Science and Technology, Qinhuangdao 066004, Hebei, China
  • Received:2017-11-13 Revised:2018-03-09 Online:2018-06-20 Published:2018-06-20

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Abstract:

To reveal the mutation type and genetic characteristics of rice mutant osnad1 (Oryza sativa nitrogen absorption deficit 1) generated by ethylmethane sulfonate (EMS) mutagenesis, we compared the phenotypes, nitrogen metabolism and photosynthesis between the mutant osnad1 and wild type (WT, Nipponbare), and conducted the genetic analysis of osnad1 in this study. The results showed that: (1) Compared with WT, the phenotype of osnad1 plant showed a decrease of plant height and root length, leaves chlorosis, and restrained growth. (2) The accumulation of total nitrogen in roots and shoots of osnad1 was significantly less than that in WT. Through determining the flow rate of nitrogen source ions (NH4 + and NO3ˉ) on root surface of osnad1 and WT by SIET technique, we found that the capacity of nitrogen absorption and the glutamine synthetase (GS) activity in osnad1 root were lower than that in WT. (3) Compared with WT, both the contents of chlorophyll and photosynthetic parameters were decreased in leaves of osnad1, causing accumulation of dry matter blocked. (4) The result of genetic analysis showed that the ratio of normal plant number to mutant plant number was 3:1. In conclusion, osnad1 was a rice mutant defective in nitrogen absorption, which was controlled by a single recessive gene. This study would provide a base for further cloning and function analysis of the osnad1 gene.

Key words: Rice, Mutant, Nitrogen absorption, Physiological analysis, Genetic analysis

Table 1

The components of nutrition and their content in the normal nutrition solution"

元素种类
Element type		 目标元素浓度
Element concentration		 使用盐类
Salt type		 盐浓度
Salt concentration
大量元素 N 2.5mM NH4NO3 1.25mM
Macroelements P 0.3mM KH2PO4 0.3mM
K 0.3+0.7mM K2SO4 0.35mM
Ca 1.0mM CaCl2 1.0mM
Mg 1.0mM MgSO4·7H2O 1.0mM
微量元素 Mn 9μM MnCl2·4H2O 9μM
Microelements Cu 0.32μM CuSO4·5H2O 0.32μM
Fe 20μM NaFeEDTA 20μM
B 20μM H3BO3 20μM
Zn 0.77μM ZnSO4·7H2O 0.77μM
Mo 0.38μM Na2MoO4·2H2O 0.38μM
Si 20μM Na2SiO3·9H2O 20μM

Fig.1

Phenotype of mutant osnad1 and its wild type Nipponbare"

Fig.2

The accumulation and concentration of total nitrogen and nitrogen physiological use efficiency in different part of osnad1 and wild type"

Fig.3

The uptake dynamic of nitrogen source ions on the root surface of osnad1 and WT in seedling stage"

Fig.4

The NR and GS activity in roots and shoots of osnad1 and WT in tillering stage"

Fig.5

The content of photosynthetic pigments and photosynthetic parameters in the flag leaf of osnad1 and WT in flowering stage"

Table 2

The genetic analysis of mutant osnad1"

组合
Cross		 F1表型
F1 phenotype		 F2群体F2 population 卡平方
χ2(3:1)
野生型
Wild type		 突变体表型
Mutant type		 总计
Total
Dular/osnad1 野生型Wild type 308 90 398 1.209
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