Crops ›› 2018, Vol. 34 ›› Issue (5): 17-20.doi: 10.16035/j.issn.1001-7283.2018.05.003

Previous Articles     Next Articles

Analysis of Peroxidase Zymogram and Genetic Effects between Rice Lines and Their Parents During Grain Filling

Ji Shengdong1,Li Peng1,Li Jiangwei2,Song Liumin1,Liu Miaomiao1,Gao Kuanglong1,Yin Haiqing3   

  1. 1 College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, China
    2 Henan Xinxiang Academy of Agricultural Sciences, Xinxiang 453000, Henan, China
    3 Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
  • Received:2018-03-08 Revised:2018-08-27 Online:2018-10-15 Published:2018-10-12
  • Contact: Haiqing Yin

RichHTML

10

PDF (PC)

195

Abstract:

The dynamic changes of peroxidase zymogram of the C and their parents at the grain filling stage were analyzed by renaturation electrophoresis. The results showed as follows: (1) The C showed a different type of new peroxidase zymogram than parents in grain filling stage, the C band type partial A and the enzyme activity changes was similar to the B. (2) The 52kD new band was detected in the mid-filling stage of C. The 53kD band was detected only in the late filling stage of the A and C, and enzyme activity of the C was weaker than that of the A. (3) 70~63kD in A, 63~58kD and 42kD in the B, both of them didn’t detect in all filling stage in C. Peroxidase zymogram differences in C resulting from parental gene interaction were expected from breeding. Further study on the relationship between the new band and the differential zymogram and the new progeny traits would provide important theoretical basis for hybrid rice breeding.

Key words: Peroxidase, Genetic effects, Renaturation electrophoresis, Rice, Parents

Table 1

Comparison of differences between parents and offspring of rice"

材料
Material		 株高(cm)
Plant height		 剑叶长(cm)
Length of
flag leaf		 穗长(cm)
Ear length		 单株有效分蘖/单株总分蘖
Effective tillers per plant/
Total tillers per plant		 穗总粒数
Grains per
panicle		 穗实粒数
Solid grains
per panicle		 千粒重(g)
1000-seed weight		 米质
Rice
quality		 生育期(d)
Growing
period		 穗颈瘟
Rice blast disease
A 105 36 23 17/20 175 163 26.11 等外 153 感
B 96 25 15 10/12 122 111 27.2 优2 165 中抗
C 98 26 19 13/15 125 113 26.27 优3 161 中抗

Fig.1

The leaf POD zymogram of rice offspring and parents in grain filling stage A, Female parent; B, Male parent; C, Offspring; The number 1, 2 and 3 were respectively in the early, middle and late stage of grain filling; M was the standard protein marker. The same below"

Fig.2

The leaf total POD activity of rice offspring and parents in grain filling stage"

[1] Yin Y, Hu J, Deng M . Latest development of antioxidant system and responses to stress in plant leaves. Chinese Agricultural Science Bulletin, 2007,23:105-130.
[2] 刘稳, 李杨, 高培基 , 等. 过氧化物酶研究进展. 饲料博览, 2009,22(11):350-356.
[3] 张俊霞, 刘晓鹏, 向极钎 , 等. 植物抗氧化系统对逆境胁迫的动态响应. 湖北民族学院学报(自然科学版), 2015(4):435-439.
[4] 王书玉, 薛应征, 陈菊霞 , 等. 水稻亲本过氧化物酶差异表达及遗传效应分析. 种子, 2009,28(12):69-71.
[5] 陈立强, 师尚礼, 马春晖 . 野生及栽培苜蓿种质资源遗传多样性的同工酶分析. 草业科学, 2014,31(6):1070-1079.
[6] 包海柱, 徐寿军, 张凤英 , 等. 模拟干旱胁迫下大麦叶片保护性酶的发育遗传分析. 华北农学报, 2016,31(6):100-104.
[7] 徐存拴, 吉爱玲 . 用复性电泳技术研究溶酶体蛋白水解酶的性质和活性. 河南科学, 1998(2):185-191.
[8] 姬生栋, 薛华政, 范红军 , 等. 玉米F1代与亲本间的过氧化物酶复性电泳分析. 河南农业科学, 2009,38(6):28-30.
[9] 尹春佳, 李彩凤, 孙世臣 , 等. 寒地超级稻叶片衰老过程中SOD、POD活性的动态变化. 作物杂志, 2009(3):37-39.
[10] 李江伟 . 导入大豆DNA的小麦变异株系灌浆后期叶片细胞衰老和POD动态研究. 郑州:河南农业大学, 2012.
[11] 杨晓娟, 唐湘如, 闻祥成 . 香稻不同生育时期叶片SOD、POD酶活性及MDA含量对播期的响应. 安徽农业科学, 2015(19):48-51.
[12] 撒多文, 米福贵, 刘玉良 . 中间偃麦草、长穗偃麦草及其杂种F1代同工酶研究. 草地学报, 2009,17(3):349-353.
[13] 姜华, 段新慧, 单贵莲 , 等. 利用POD同工酶分析紫花苜蓿亲本及杂种后代遗传变异. 草地学报, 2015,23(2):372-376.
[14] 程广有, 韦立文 . 杨树品种过氧化物同工酶分析. 北华大学学报(自然科学版), 2000(6):529-532.
[15] 杨凯 . 玉米杂交种及其亲本籽粒基因差异表达与杂种优势关系的研究. 呼和浩特:内蒙古农业大学, 2012.
[16] Schwartz D . Genetic studies on mutant enzymes in maize:Synthesis of hybird enzymes by heterozygotes. Proceedings of the National Academy of Sciences of the United States of America, 1960,46(9):1210-1215.
doi: 10.1073/pnas.46.9.1210
[17] 黄夕洋, 闫海锋, 李虹 , 等. 三倍体罗汉果及其亲本的同工酶比较研究. 广西植物, 2015(6):802-806.
[18] 尹明智, 官春云 . 油菜新型胞质雄性不育系1193A和保持系的同工酶分析. 作物杂志, 2016(4):36-40.
[1] Ma Mengli,Zheng Yun,Zhou Xiaomei,Zhang Tingting,Zhang Xiaoqian,Lu Bingyue. Genetic Diversity Analysis of Red Rice from Hani’s Terraced Fields in Yunnan Province [J]. Crops, 2018, 34(5): 21-26.
[2] Chen Yingying,Wangxu Yiling,Zhu Yuhan,Wu Wei,Liu Tao,Sun Chengming. Hyperspectral Estimation of Nitrogen Content in Rice Panicle [J]. Crops, 2018, 34(5): 116-120.
[3] Xiaoyu Liang, Chunyu Lin, Shumei Ma, Yang Wang. Mining Elite Alleles for Germination Ability in Rice (Oryza sativa L.) under Salt and Alkaline Stress [J]. Crops, 2018, 34(4): 48-52.
[4] Xingchuan Zhang, Wenxuan Huang, Kuanyu Zhu, Zhiqin Wang, Jianchang Yang. Effects of Nitrogen Rates on the Nitrogen Use Efficiency and Agronomic Traits of Different Rice Cultivars [J]. Crops, 2018, 34(4): 69-78.
[5] Bo Zeng. Renovation of Main Cultivated Rice Varieties in China in the Past 30 Years [J]. Crops, 2018, 34(3): 1-7.
[6] Lili Zhang,Yizhou Zhao,Xin Li,Ting Mao,Yan Liu,Zhan Zhang,Shanjun Ni,Fucai Liu. Mutant Analysis on Quality Trait of Different Japonica Rice Progenies Induced by 60Co-γ Ray Irradiation [J]. Crops, 2018, 34(3): 51-56.
[7] Li Zhang,Zantang Li,Shiyin Wang,Yanchao Ma,Yang Dongfang,Xueyong Li,Jiang Xu. Physiological and Genetic Analysis of Rice Mutant osnad1 Defective in Nitrogen Absorption [J]. Crops, 2018, 34(3): 68-76.
[8] Chen He,Guiping Zheng,Haicheng Zhao,Liqiang Chen,Hongyu Li,Yandong Lü,Jiang Song. Effects of Increasing Humic Acid but Reducing Fertilization on Panicle Traits and Yield of Rice in Saline-Alkali Soil [J]. Crops, 2018, 34(3): 129-134.
[9] Yong Cui. The Research Progress of Water-Dry Rotation Methods in Paddy Field [J]. Crops, 2018, 34(3): 8-14.
[10] Zhiqiang Tang,Liqiang Dong,Rui Li,Liying Zhang,Na He,Yuedong Li. Effects of Nitrogen and Soil Type on Seedling Quality and Nutrient Absorption in Rice [J]. Crops, 2018, 34(3): 141-147.
[11] Bo Zeng,Shixian Sun,Jie Wang. Registration of Main Rice Varieties and Its Application in Recent 30 Years in China [J]. Crops, 2018, 34(2): 1-5.
[12] Zhibo Zhou,Yake Yi,Guanghui Chen. Effects of Sowing Amount, Medium and Chemical Treatment on Seedling Quality and Yield of High Quality Late-Rice Variety of Yuzhenxiang [J]. Crops, 2018, 34(2): 129-135.
[13] Ge Qu,Zhengguang Chen,Xue Wang. Identification of Rice Varieties Using NIR Spectroscopy and SIMCA, PLS-DA Methods [J]. Crops, 2018, 34(2): 166-170.
[14] Jianjun Wang,Yongjun Zeng,Yanhong Yi,Qiming Zhang,Qixing Hu,Xueming Tan,Shan Huang,Qingyin Shang,Yanhua Zeng,Qinghua Shi. The Uniformity of Mechanical-Transplanted Early-Season Rice under Different Seeding Rates and Its Effects on the Formation of Grain Yield [J]. Crops, 2018, 34(2): 141-147.
[15] Suihua Huang,Haowen Luo,Xingge Huang,Pingshan Fan,Leilei Kong,Zhuojun Zhong,Xiangru Tang. Effects of Seeds Dressing Agent on Morphologyand Biochemical Characteristics of Direct-Sowing Rice Seeding [J]. Crops, 2018, 34(2): 171-176.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production[J]. Crops, 2018, 34(4): 1 -7 .
[2] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut[J]. Crops, 2018, 34(4): 102 -105 .
[3] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[4] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[5] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System[J]. Crops, 2018, 34(4): 114 -120 .
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation[J]. Crops, 2018, 34(4): 121 -125 .
[7] Xiaofei Wang,Haijun Xu,Mengqiao Guo,Yu Xiao,Xinyu Cheng,Shuxia Liu,Xiangjun Guan,Yaokun Wu,Weihua Zhao,Guojiang Wei. Effects of Sowing Date, Density and Fertilizer Utilization Rate on the Yield of Oilseed Perilla frutescens in Cold Area[J]. Crops, 2018, 34(4): 126 -130 .
[8] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[9] Jie Gao,Qingfeng Li,Qiu Peng,Xiaoyan Jiao,Jinsong Wang. Effects of Different Nutrient Combinations on Plant Production and Nitrogen, Phosphorus and Potassium Utilization Characteristics in Waxy Sorghum[J]. Crops, 2018, 34(4): 138 -142 .
[10] Na Shang,Zhongxu Yang,Qiuzhi Li,Huihui Yin,Shihong Wang,Haitao Li,Tong Li,Han Zhang. Response of Cotton with Vegetative Branches to Plant Density in the Western of Shandong Province[J]. Crops, 2018, 34(4): 143 -148 .