Crops ›› 2018, Vol. 34 ›› Issue (6): 116-123.doi: 10.16035/j.issn.1001-7283.2018.06.018

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Effects of Exogenous Hydrogen Peroxide on Floral Bud Differentiation in Tobacco

Wang Ning,Zhang Jing,Huang Jinyong,Shi Tuansheng,Du Jian,Yue Caipeng   

  1. School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China
  • Received:2018-09-30 Online:2018-12-15 Published:2018-12-06

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Abstract:

The active oxygen metabolism and flower bud differentiation in tobacco were studied by spraying exogenous H2O2 via pot experiments. Results showed that: (1) The active oxygen metabolism was significantly changed in tobacco by spraying H2O2. After spraying H2O2, the content of H2O2 and O2 _ was rapidly increased in tobacco, the activities of POD and CAT were rapidly activated followed by the activation of SOD. In the middle and latter period of spraying, the content of O2 _ was high, while the content of H2O2 was significantly decreased. (2) The expressions of the floral development genes were affected, the number of leaves in squaring stage was altered and the process of flower bud development was modified by spraying of H2O2. The expression of FLC gene was significantly inhibited while the expression of LFY gene was significantly promoted in tobaccos which were sprayed H2O2 at 5 and 15 consecutive days. Floral bud differentiation of tobaccos progressed earlier under 5 to 25 consecutive days spray of H2O2. The flowering transition was affected by the change of active oxygen equilibrium in tobacco.

Key words: Tobacco, Exogenous hydrogen peroxide, Reactive oxygen species, Floral bud differentiation

Fig.1

Rapidly responsive changes of O2- content in tobacco treated with exogenous H2O2 *, Significant difference at 0.05 level; **, Extremely significant difference at 0.01 level; the same below"

Fig.2

Changes of O2- content in tobacco treated with exogenous H2O2 for a long time"

Fig.3

Rapidly responsive changes of H2O2 content in tobacco treated with exogenous H2O2"

Fig.4

Changes of H2O2 content in tobacco treated with exogenous H2O2 for a long time"

Fig.5

Rapid response changes of POD activity in tobacco treated with exogenous H2O2"

Fig.6

Changes of POD activity in tobacco treated with exogenous H2O2 for a long time"

Fig.7

Rapidly responsive changes of CAT activity in tobacco treated with exogenous H2O2"

Fig.8

Changes of CAT activity in tobacco treated with exogenous H2O2 for a long time"

Fig.9

Rapidly responsive changes of SOD activity in tobacco treated with exogenous H2O2"

Fig.10

Changes of SOD activity in tobacco treated with exogenous H2O2 for a long time"

Fig.11

Effect of exogenous H2O2 on the expression of FLC"

Fig.12

Effect of exogenous H2O2 on the expression of LFY"

Fig.13

The flower bud differentiation of tobacco under exogenous H2O2 conditions (10×5）"

Fig.14

The leaf number of tobacco when buds occur under exogenous H2O2"

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