Crops ›› 2017, Vol. 33 ›› Issue (5): 156-161.doi: 10.16035/j.issn.1001-7283.2017.05.026

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Effects of Cadmium Stress on Agronomic Traits and Physiological and Biochemical Indexes of Flue-Cured Tobacco

Yan Han1,Song Hanglin2,Zhang Li1,Yan Jing1,Shi Xianji1,Zhu Shimiao1,Liu Lu1,Li Hulin1   

  1. 1Agronomy College,Yanbian University,Yanji 133002,Jilin,China
    2Yanbian Academy of Agricultural Sciences,Longjing 133400,Jilin,China
  • Received:2017-05-17 Revised:2017-07-26 Online:2017-10-15 Published:2018-08-26
  • Contact: Lu Liu

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Abstract:

In order to clarify the effects of heavy metal (Cd) stress on agronomic traits and physiological and biochemical indexes of flue-cured tobacco, the agronomic traits and physiological and biochemical indexes were determined under different concentration Cd stress for cultivated species Jiyan 9 of flue-cured tobacco using potting method in the experiment. The results showed that, leaf length, leaf width, plant height and stem circumference decreased when Cd>3mg/kg (except the leaf width of Cd20 treatment). The activity of catalase (CAT) and superoxide dismutase (SOD) increased first and then decreased with the increase of Cd concentration, which was opposite to the content of proline (Pro). The activity of peroxidase (POD) and content of Malondialdehyde (MDA) increased while the content of chlorophyll (Chl) decreased continuously, the content of total nitrogen, protein, total sugar and reducing sugar increased first and then decreased but then increased again with the increase of Cd concentration. The content of nicotine decreased with the increase of Cd concentration.

Key words: Flue-cured tobacco, Cd stress, Agronomic traits, Physiological and biochemical indexes

Table 1

Agronomic characters at 40 days after sewage irrigation"

处理Treatment 叶长Leaf length (cm) 叶宽Leaf width (cm) 株高Plant height (cm) 茎围Stem girth (cm) 叶片数Leaf No. (个)
CK 59.96±1.73bABC 25.44±0.00abA 110.00±0.75bBC 7.80±0.06abA 19.78±1.03abA
Cd1 62.00±1.15abAB 26.60±0.35aA 114.47±0.73aAB 7.93±0.04abA 18.67±0.73bA
Cd3 65.67±0.19aA 29.73±1.34aA 116.33±1.73aA 8.13±0.08aA 19.67±0.19abA
Cd6 60.13±0.08bABC 26.60±1.33aA 110.33±0.81bBC 7.80±0.46abA 20.00±0.00abA
Cd10 59.53±3.00bABC 24.67±0.79abA 110.00±0.58bBC 7.67±0.68abA 19.33±0.19abA
Cd20 58.00±0.58bcBC 24.73±3.13bA 108.67±1.39bC 7.33±0.19abA 20.67±0.06aA
Cd40 54.80±0.35cC 24.20±1.21abA 94.67±0.39cD 6.87±0.06bA 19.00±0.00bA

Fig.1

Effect of Cd stress on chlorophyll content"

Fig.2

Effect of Cd stress on CAT activity"

Fig.3

Effect of Cd stress on POD activity"

Fig.4

Effect of Cd stress on SOD activity"

Fig.5

Effect of Cd stress on MDA content"

Fig.6

Effect of Cd stress on soluble sugar content"

Fig.7

Effect of Cd stress on Pro content"

Table 2

Significant analysis of the physiological indexes"

浓度Concentration Chl含量
Chl content
(μg/gFW)		 CAT活性
CAT activity
[U/(min·gFW)]		 POD活性
POD activity
[U/(min·gFW)]		 SOD活性
SOD activity
[U/(min·gFW)]		 MDA含量
MDA content
(μmol/gFW)		 可溶性糖含量
Soluble sugar content
(μmol/gFW)		 Pro含量
Pro content
(μg/gFW)
CK 41.02±0.07aA 41.24±0.13cC 307.93±0.17gG 135.84±0.06gG 9.10±0.71eE 11.97±0.16dC 310.72±0.09aA
Cd1 38.01±1.44bB 54.50±0.00bB 314.94±0.23fF 160.67±0.12bfF 10.03±0.15dDE 12.91±0.01cBC 288.47±0.04cC
Cd3 34.26±0.26cC 56.46±0.03aA 327.86±0.36eE 238.51±0.09bB 11.14±0.20cCD 14.00±0.58bB 275.64±0.19fF
Cd6 32.46±0.14cCD 24.26±0.03dD 341.84±0.15dD 277.29±0.10aA 11.52±0.17cC 17.85±0.29aA 251.49±0.01gG
Cd10 30.31±0.03dDE 16.13±0.01eE 390.13±0.08cC 205.83±0.03cC 13.00±0.06bB 16.99±0.10aA 276.58±0.12eE
Cd20 28.31±0.18eEF 12.69±0.10fF 402.16±0.29bB 202.45±0.17dD 13.61±0.01bB 17.00±0.13aA 286.58±0.05dD
Cd40 27.34±0.52eF 9.94±0.31gG 458.54±0.19aA 188.27±0.05eE 16.57±0.13aA 17.25±0.42aA 295.09±0.28bB

Table 3

Significant analysis for chemical composition of upper, middle and lower leaves %"

部位Position 处理Treatment 总氮Total nitrogen 蛋白质Protein 烟碱Nicotine 还原糖Reducing sugar 总糖Total sugar
上部叶Upper leaf CK 2.08aA 9.05bB 2.59aA 17.36fD 20.34dD
Cd1 2.14aA 9.48bB 2.31bB 20.36bB 20.50cdCD
Cd3 2.26aA 10.26aA 2.20bcBC 25.21aA 27.05aA
Cd6 2.27aA 10.33aA 1.99cdC 19.98cB 22.17bB
Cd10 2.11aA 9.25bB 1.97dC 19.94cB 22.09bB
Cd20 2.11aA 9.24bB 1.91dC 17.94eC 20.62cCD
Cd40 2.11aA 9.25bB 1.89dC 18.34dC 20.74cC
中部叶Middle leaf CK 2.13aA 9.26cB 2.50aA 18.66cC 20.99bB
Cd1 2.23aA 9.84bcAB 2.33aA 18.73cC 21.00bB
Cd3 2.34aA 10.51abA 2.10aA 26.48aA 28.14aA
Cd6 2.36aA 10.79aA 1.88aA 20.84bB 21.86bB
Cd10 2.23aA 9.98abcAB 1.85aA 20.81bB 21.81bB
Cd20 2.23aA 9.90bcAB 1.80aA 18.81cC 21.04bB
Cd40 2.30aA 10.24abAB 1.80aA 19.31cC 21.55bB
下部叶Lower leaf CK 2.02aA 9.00bC 1.72aA 16.78eE 21.07dD
Cd1 2.19aA 10.09abAB 1.53abAB 21.31bB 27.07aA
Cd3 2.28aA 10.66aA 1.34bcBC 24.52aA 27.43aA
Cd6 2.29aA 10.71aA 1.12cCD 19.90cC 24.94bB
Cd10 2.12aA 9.61abBC 1.09cD 19.88cC 24.24bB
Cd20 2.12aA 9.62abBC 1.04cD 17.82dD 21.81cdD
Cd40 2.21aA 10.23abAB 1.03cD 18.35dD 22.83cC
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