Crops ›› 2020, Vol. 36 ›› Issue (4): 188-194.doi: 10.16035/j.issn.1001-7283.2020.04.027

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Estimation of Crude Protein Content in Grain of Early Indica Rice Based on Canopy Spectrum

Tian Rongcai(), Gao Zhiqiang, Lu Junwei()   

  1. Agronomy College of Hunan Agricultural University, Changsha 410128, Hunan, China
  • Received:2019-12-30 Revised:2020-05-01 Online:2020-08-15 Published:2020-08-11
  • Contact: Lu Junwei E-mail:823945102@qq.com;345321@qq.com

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Abstract:

In order to find the most suitable model of estimating the crude protein content of grains, we took the germplasm resources of early and middle maturity groups of early indica rice varieties in the middle and lower regimes of the Yangtze River in 2019 as materials, analyzed the relationships between the canopy spectra reflectance at the main growth stages and the crude protein content of grains, and also screened out the optimal period and sensitive wave length that could be used to predict the crude protein content in the early indica rice varieties. The univariate linear, multivariate linear, exponential, and polynomial prediction models for the crude protein content of grains based on sensitive wave lengths and spectra parameters were established, and the determination coefficient (R 2), root mean square error (RMSE) and relative error (RE) were used to evaluate the accuracy of the models, for finding the best model to estimate the crude protein content of early indica rice. The results showed that the correlation between the first derivative spectral reflectance of the canopy and the crude protein content of the grain at 514, 580, 638 and 695nm reached an extremely significant level at booting stage. Among the estimation models based on sensitive wave length, the quaternary linear model had the best estimation effects, with 0.566, 0.342% and 2.874% for the calibration set R 2, RMSE and RE, and 0.518, 0.154% and 1.303% for the validation set R 2, RMSE and RE, respectively. In the estimation model that constructed based on the spectra parameters, the polynomial model which constructed by DSI (R′514, R′638) as the independent variable had a better estimation effects, and its calibration set R 2, RMSE and RE were 0.638, 0.312% and 2.639%, respectively, the validation set R 2, RMSE and RE were 0.581, 0.230% and 2.307%, respectively.

Key words: Early indica rice, Crude protein content, Spectrum, Model

Table 1

Spectral index calculation formula"

光谱指数
Spectral index		 名称Name 公式
Formula
NDSI 归一化差值光谱指数
Normalized difference spectral index		 (Rλ1-Rλ2)/(Rλ1+Rλ2)
DSI 差值光谱指数
Difference spectral index		 Rλ1-Rλ2
RSI 比值光谱指数Ratio spectral index Rλ1/Rλ2

Fig.1

Spectral curves of different early indica rice varieties"

Fig.2

Original (A) and first derivative (B) spectral reflectance of early indica rice at different growth stages"

Fig.3

The correlation coefficients between canopy original (A) and first derivative (B) spectral reflectance and grain crude protein content in early indica rice during different growth periods"

Table 2

The estimation of grain crude protein content of early indica rice based on sensitive wavelength"

波长及组合Wavelength and its combination 估测模型Estimating model R2 RMSE (%) RE (%)
R′514 y=4257.309R′514+5.885 0.503 0.366 3.294
R′580 y=-6997.267R′580+6.476 0.468 0.378 3.400
R′638 y=-10457.111R′638+7.341 0.538 0.353 2.870
R′695 y=1456.315R′695+6.021 0.462 0.380 3.465
(R′514,R′580) y=3253.278R′514-1841.650R′580+5.935 0.508 0.364 3.312
(R′514,R′638) y=1784.503R′514-6786.287R′638+6.566 0.560 0.344 2.962
(R′514,R′695) y=4186.184R′514+26.538R′695+5.882 0.503 0.366 3.295
(R′580,R′638) y=-2061.051R′580-7981.623R′638+6.946 0.548 0.349 2.967
(R′580,R′695) y=-3918.876R′580+691.696R′695+6.145 0.481 0.373 3.414
(R′638,R′695) y=-8410.792R′638+351.739R′695+6.911 0.544 0.350 2.967
(R′514,R′580,R′638) y=1881.122R′514+225.038R′580-6857.824R′638+6.567 0.560 0.344 2.956
(R′514,R′580,R′695) y=3623.759R′514-2138.226R′580-198.561R′695+5.968 0.508 0.364 3.309
(R′514,R′638,R′695) y=3087.336R′514-7494.482R′638-582.393R′695+6.712 0.565 0.342 2.868
(R′580,R′638,R′695) y=-1884.608R′580-7883.872R′638+53.229R′695+6.915 0.548 0.349 2.974
(R′514,R′580,R′638,R′695) y=2956.682R′514-562.819R′580-7375.90R′638-632.008R′695+6.721 0.566 0.342 2.874

Table 3

The estimation of grain crude protein content of early indica rice based on spectral parameters"

光谱参数Parameter 模型Model 回归方程Regression equation R2 RMSE (%) RE (%)
NDSI(R′638,R′695) 线性Linear y=15.3x-8.556 0.356 0.416 3.535
指数Exponential y=1.408e1.616x 0.362 0.416 3.544
多项式Polynomial y=44.553x2-90.223x+53.9 0.359 0.415 3.584
DSI(R′514,R′638) 线性Linear y=-3205.643x+6.101 0.544 0.368 3.059
指数Exponential y=6.626e-338.051x 0.550 0.354 3.151
多项式Polynomial y=-10340000x2-26457.474x-6.826 0.638 0.312 2.639
RSI(R′638,R′695) 线性Linear y=0.242x+12.494 0.342 0.421 3.491
指数Exponential y=13.013e0.026x 0.348 0.422 3.585
多项式Polynomial y=0.036x2+1.135x+17.92 0.358 0.415 3.572

Fig.4

Accuracy test of crude protein content estimation model for early indica rice seeds based on sensitive wavelength (A) and spectral parameter (B)"

[1] Raubenheimer D, Simpson S J . Nutritional ecology and human health. Annual Review of Nutrition, 2016,36:603-626.
[2] 彭波, 孙艳芳, 韩秋 , 等. 水稻种子蛋白质含量检测方法的比较分析. 江苏农业科学, 2018,46(4):22-28.
[3] 贺佳, 刘冰峰, 黎世民 , 等. 不同生育时期冬小麦籽粒蛋白质含量的高光谱遥感监测模型. 中国生态农业学报, 2017,25(6):865-875.
[4] 张晗, 赵小敏, 郭熙 , 等. 基于冠层高光谱信息的水稻生长监测应用研究进展. 江苏农业科学, 2018,46(12):1-9.
[5] 吴琼, 齐波, 赵团结 , 等. 高光谱遥感估测大豆冠层生长和籽粒产量的探讨. 作物学报, 2013,39(2):309-318.
[6] 高鑫 . 春玉米LAI和叶片氮素营养及产量的高光谱估测模型研究. 呼和浩特:内蒙古农业大学, 2016.
[7] 李岚涛, 任涛, 汪善勤 , 等. 基于角果期高光谱的冬油菜产量预测模型研究. 农业机械学报, 2017,48(3):221-229.
[8] 李媛媛, 常庆瑞, 刘秀英 , 等. 基于高光谱和BP神经网络的玉米叶片SPAD值遥感估算. 农业工程学报, 2016,32(16):135-142.
[9] Rodrigues Jr F A, Blasch G, Defourny P , et al. Multi-temporal and spectral analysis of high-resolution airborne imagery for precision agriculture:assessment of wheat grain yield and grain protein content. Remote Sensing, 2018,10(6):930-955.
[10] 周冬琴, 朱艳, 姚霞 , 等. 基于水稻冠层光谱特征构建粳型水稻籽粒蛋白质含量预测模型. 作物学报, 2007,33(8):1219-1225.
[11] 薛利红, 曹卫星, 李映雪 , 等. 水稻冠层反射光谱特征与籽粒品质指标的相关性研究. 中国水稻科学, 2004,18(5):57-62.
[12] Onoyama H, Ryu C, Suguri M , et al. Estimation of rice protein content before harvest using ground-based hyperspectral imaging and region of interest analysis. Precision Agriculture, 2018,19:721-734.
[13] Song X Y, Yang G J, Yang C H , et al. Spatial variability analysis of within-field winter wheat nitrogen and grain quality using canopy fluorescence sensor measurements. Remote Sensing, 2017,9(3):237-254.
[14] 孙雪梅, 周启发, 何秋霞 . 利用高光谱参数预测水稻叶片叶绿素和籽粒蛋白质含量. 作物学报, 2005,31(7):844-850.
[15] 李永梅, 张立根, 张学俭 . 水稻叶片高光谱响应特征及氮素估算. 江苏农业科学, 2017,45(23):210-213.
[16] 于滋洋, 王翔, 孟祥添 , 等. 考虑水分光谱吸收特征的水稻叶片SPAD预测模型. 光谱学与光谱分析, 2019,39(8):2528-2532.
[17] 刘桃菊, 张笑东, 胡雯君 , 等. 水稻冠层光谱红边特征及其与叶片氮素营养状况的关系. 2014年中国作物学会学术年会论文集, 南京:中国作物学会, 2014: 131.
[18] 谢晓金, 李秉柏, 朱红霞 . 利用高光谱数据估测不同温度胁迫下的水稻籽粒中粗蛋白和直链淀粉含量. 农业现代化研究, 2012,33(4):481-484.
[19] 张浩, 胡昊, 陈义 , 等. 水稻叶片氮素及籽粒蛋白质含量的高光谱估测模型. 核农学报, 2012,26(1):135-140.
[20] Ryu C, Suguri M, Iida M , et al. Integrating remote sensing and gis for prediction of rice protein contents. Precision Agriculture, 2011(3):378-394.
[21] Asaka D, Shiga H . Estimating rice grain protein contents with SPOT/HRV data acquired at maturing stage. Journal of the Remote Sensing Society of Japan, 2003,23(5):451-457.
[22] 唐延林, 黄敬峰, 王人潮 . 利用高光谱法估测稻穗稻谷的粗蛋白质和粗淀粉含量. 中国农业科学, 2004,37(9):1282-1287.
[23] 张浩, 姚旭国, 张小斌 , 等. 水稻籽粒蛋白质含量的高光谱估测研究. 中国粮油学报, 2009,24(11):1-5.
[24] 仲晓春, 何理, 陈莹莹 , 等. 基于高光谱的稻米品质估测模型的构建. 扬州大学学报(农业与生命科学版), 2012,33(2):34-38.
[25] 陈瑛瑛, 王徐艺凌, 朱宇涵 , 等. 水稻穗部氮素含量高光谱估测研究. 作物杂志, 2018(5):116-120.
[26] 丁锦峰, 朱新开, 王君婵 , 等. 基于开花期卫星影像的春性中、弱筋小麦籽粒蛋白质含量遥感预测. 云南农业大学学报(自然科学), 2015,30(6):932-940.
[27] 李振海, 杨贵军, 王纪华 , 等. 作物籽粒蛋白质含量遥感监测预报研究进展. 中国农业信息, 2018,30(1):46-54.
[28] Li Z H, Taylor J, Yang H , et al. A hierarchical interannual wheat yield and grain protein prediction model using spectral vegetative indices and meteorological data. Field Crops Research, 2020,248:107711-107718.
[29] 李振海, 徐新刚, 金秀良 , 等. 基于氮素运转原理和GRA-PLS算法的冬小麦籽粒蛋白质含量遥感预测. 中国农业科学, 2014,47(19):3780-3790.
[30] 依尔夏提·阿不来提, 买买提·沙吾提, 白灯莎·买买提艾力 , 等. 基于随机森林法的棉花叶片叶绿素含量估算. 作物学报, 2019,45(1):81-90.
[31] Siegmann B, Jarmer T . Comparison of different regression models and validation techniques for the assessment of wheat leaf area index from hyperspectral data. International Journal of Remote Sensing, 2015,36(18):4519-4534.
[32] Wang L, Chang Q R, Li F L , et al. Effects of growth stage development on paddy rice leaf area index prediction models. Remote Sensing, 2019,11(3):361-378.
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