Crops ›› 2017, Vol. 33 ›› Issue (3): 13-18.doi: 10.16035/j.issn.1001-7283.2017.03.003

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Quantitative Genetic Research of Plant Height and Ear Height in Maize under Different Environments

He Wenzhao,Wang Hongwu,Hu Xiaojiao,Li Kun,Wang Qi,Wu Yujin,Liu Zhifang,Huang Changling   

  1. Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2017-04-02 Revised:2017-05-04 Online:2017-06-15 Published:2018-08-26
  • Contact: Hongwu Wang,Changling Huang

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Abstract:

Using a recombinant inbred line (RIL) population derived from the cross between Zhongdan 909’s parental lines Zheng 58 and HD568, we studied the genetic characters of plant height and ear height of maize under different environments. Analysis of phenotype data from RIL population and their parents showed that plant height and ear height had high broad sense heritability, but still significantly affected by environment factors. The joint segregation analysis of the three generations revealed that the plant height fitted the genetic model of F-2 in environment Ⅰ (Xinxiang,Henan; Summer of 2015) and genetic model of G-0 in both environments Ⅱ (Xinxiang, Henan; Summer of 2016) and Ⅲ(Gongzhuling, Jilin; Spring of 2016); Ear height fitted the genetic model of F-2 in environments Ⅰ and Ⅱ, and genetic model G-0 in environment Ⅲ. The results indicated that environment factors had a significant effect on the genetic models of these two traits. Study of the genetic laws of plant height and ear height under different environments will provide a theoretical basis for QTL mapping and breeding maize varieties with wide adaptability.

Key words: Maize, Plant height, Ear height, Genetic model

Table 1

The phenotypic analysis of parents and RIL population in three environments m"

环境
Environment		 性状
Trait		 亲本 Parent RIL群体RIL population
郑58 Zheng 58 HD568 均值±标准误Mean±SE 峰度Kurtosis 偏度Skewness 变异系数CV (%)
Ⅰ 株高Plant height 1.54 1.82 1.69±0.01 -0.27 -0.07 11.93
穗位高Ear height 0.54 0.71 0.61±0.01 -0.06 0.27 18.75
Ⅱ 株高Plant height 1.54 1.76 1.65±0.01 -0.49 -0.30 11.61
穗位高Ear height 0.50 0.65 0.57±0.01 0.11 0.32 18.90
Ⅲ 株高Plant height 1.91 1.99 1.96±0.00 0.14 -0.08 11.14
穗位高Ear height 0.64 0.79 0.78±0.01 0.35 0.11 17.93

Table 2

The joint variance analysis of plant height and ear height of populations"

变异来源Source of variation 株高Plant height 穗位高Ear height
自由度DF 均方MS F值F value 自由度DF 均方MS F值F value
环境Environment 2 12.100330 2 123.96** 2 5.315545 2 057.54**
家系Genealogy 217 0.210972 37.03** 217 0.071884 27.82**
重复Repeat 1 0.002425 0.43 1 0.000103 0.04
环境×家系Environment×Genealogy 430 0.018232 3.20** 430 0.007909 3.06**
环境×重复Environment×Repeat 2 0.094939 16.66** 2 0.064114 24.82**
重复×家系Repeat×Genealogy 217 0.007113 1.25 217 0.002777 1.07
广义遗传力Generalized heritability (%) 91.35 88.99

Table 3

AIC of plant height in three environments"

模型代码
Model code		 环境Environment 模型代码
Model code		 环境Environment
Ⅰ Ⅱ Ⅲ Ⅰ Ⅱ Ⅲ
A-0 2 139.916 2 191.265 2 268.605 E-5 2 078.848 2 127.724 2 205.402
A-1 2 092.312 2 146.722 2 215.780 E-6 2 078.864 2 130.288 2 207.599
B-1 2 094.458 2 147.159 2 217.372 E-7 2 076.865 2 129.057 2 206.060
B-2 2 094.010 2 148.550 2 217.758 E-8 2 076.865 2 129.057 2 206.060
B-3 2 092.010 2 146.550 2 215.758 E-9 2 076.862 2 128.281 2 205.599
B-4 2 094.287 2 148.705 2 217.776 F-1 2 093.528 2 082.453 2 152.275
B-5 2 092.474 2 145.166 2 215.372 F-2 2 061.712 2 044.444 2 083.405
B-6 2 092.458 2 145.159 2 215.372 F-3 2 082.937 2 070.834 2 140.346
B-7 2 090.480 2 143.168 2 213.372 F-4 2 093.802 2 148.443 2 217.753
B-8 2 092.454 2 146.803 2 215.794 G-0 2 075.179 1 993.764 2 060.324
B-9 2 092.734 2 143.101 2 212.494 G-1 2 088.050 2 060.419 2 121.378
C-0 2 082.339 2 130.894 2 208.449 G-2 2 088.450 2 142.562 2 218.722
C-1 2 080.428 2 131.696 2 207.912 G-3 2 076.909 2 130.818 2 206.895
D-1 2 078.865 2 130.213 2 207.553 G-4 2 088.478 2 142.540 2 219.055
D-2 2 076.908 2 130.836 2 206.897 H-1 2 094.535 2 120.893 2 199.099
E-0 2 081.802 2 130.934 2 208.771 H-2 2 087.815 2 122.364 2 216.871
E-1 2 080.863 2 129.489 2 207.244 H-3 2 091.661 2 146.380 2 215.754
E-2 2 078.909 2 132.828 2 208.896 H-4 2 093.663 2 148.381 2 217.755
E-3 2 076.909 2 130.827 2 206.896 H-5 2 093.671 2 147.975 2 217.406
E-4 2 078.871 2 127.724 2 205.402

Table 4

The goodness of fit test of genetic model for plant height"

环境Environment 模型Mode 群体Population U12 U22 U32 nW2 Dn
Ⅰ F-2 P1 4.69(0.03) 2.45(0.12) 4.56(0.03) 0.54(<0.05) 0.93(<0.05)
P2 5.80(0.02) 2.50(0.11) 9.02(0.00) 0.65(<0.05) 0.99(<0.05)
RIL 0.61(0.44) 0(0.95) 7.56(0.01) 0.23(>0.05) 0.03(>0.05)
Ⅱ F-2 P1 3.34(0.07) 2.26(0.13) 1.13(0.29) 0.43(>0.05) 0.86(<0.05)
P2 2.22(0.14) 1.95(0.16) 0.03(0.86) 0.33(>0.05) 0.77(>0.05)
RIL 0.00(0.96) 0.25(0.62) 4.45(0.03) 0.13(>0.05) 0.02(>0.05)
G-0 P1 0(1) 0.05(0.82) 0.80(0.37) 0.07(>0.05) 0.36(>0.05)
P2 0(1) 0.01(0.93) 0.11(0.74) 0.05(>0.05) 0.32(>0.05)
RIL 0.25(0.62) 0.55(0.46) 1.06(0.30) 0.15(>0.05) 0.01(>0.05)
Ⅲ F-2 P1 0.69(0.41) 1.07(0.30) 0.85(0.36) 0.16(>0.05) 0.59(>0.05)
P2 3.40(0.07) 2.26(0.13) 1.28(0.26) 0.42(>0.05) 0.85(<0.05)
RIL 0.01(0.91) 0.26(0.61) 2.56(0.11) 0.09(>0.05) 0.00(>0.05)
G-0 P1 0(1) 0.07(0.79) 1.15(0.28) 0.07(>0.05) 0.37(>0.05)
P2 0(1) 0.00(0.98) 0.01(0.93) 0.05(>0.05) 0.30(>0.05)
RIL 0.04(0.84) 0.03(0.86) 0.01(0.94) 0.05(>0.05) 0.00(>0.05)

Table 5

AIC of ear height in three environments"

模型代码
Model code		 环境Environment 模型代码
Model code		 环境Envitonment
Ⅰ Ⅱ Ⅲ Ⅰ Ⅱ Ⅲ
A-0 1 835.468 1 847.213 1 966.698 E-5 1 774.078 1 784.986 1 903.061
A-1 1 779.063 1 790.235 1 915.104 E-6 1 774.553 1 786.200 1 903.223
B-1 1 782.112 1 792.191 1 915.481 E-7 1 773.314 1 784.586 1 900.961
B-2 1 780.514 1 791.819 1 916.873 E-8 1 773.313 1 784.586 1 900.961
B-3 1 778.514 1 789.826 1 914.873 E-9 1 772.491 1 784.196 1 903.130
B-4 1 780.927 1 792.179 1 917.103 F-1 1 788.007 1 792.090 1 902.219
B-5 1 780.248 1 790.235 1 913.482 F-2 1 765.586 1 758.921 1 893.372
B-6 1 780.112 1 790.191 1 913.481 F-3 1 776.683 1 781.170 1 895.952
B-7 1 778.301 1 788.251 1 911.482 F-4 1 780.204 1 791.580 1 916.759
B-8 1 779.104 1 790.381 1 915.393 G-0 1 783.438 1 779.353 1 861.105
B-9 1 779.223 1 789.122 1 915.449 G-1 1 781.481 1 795.126 1 902.249
C-0 1 773.737 1 785.009 1 904.627 G-2 1 789.977 1 798.374 1 933.262
C-1 1 774.246 1 784.195 1 911.481 G-3 1 774.866 1 785.317 1 912.535
D-1 1 774.419 1 786.163 1 905.648 G-4 1 789.971 1 798.344 1 933.257
D-2 1 774.870 1 785.318 1 912.546 H-1 1 790.813 1 791.743 1 911.049
E-0 1 778.026 1 788.785 1 907.467 H-2 1 777.609 1 778.617 1 918.277
E-1 1 776.058 1 786.927 1 905.223 H-3 1 778.046 1 789.455 1 914.709
E-2 1 776.868 1 787.317 1 914.542 H-4 1 780.047 1 791.455 1 916.711
E-3 1 774.868 1 785.318 1 912.540 H-5 1 780.011 1 791.320 1 916.519
E-4 1 774.078 1 784.986 1 903.061

Table 6

The goodness of fit test of genetic model for ear height"

环境Environment 模型Mode 环境Population U12 U22 U32 nW2 Dn
Ⅰ F-2 P1 4.63(0.03) 2.44(0.12) 4.33(0.04) 0.54(<0.05) 0.92(<0.05)
P2 3.15(0.08) 2.12(0.15) 1.10(0.29) 0.36(>0.05) 0.78(>0.05)
RIL 0.13(0.72) 0(0.95) 1.25(0.26) 0.05(>0.05) 0(>0.05)
Ⅱ F-2 P1 3.73(0.05) 2.28(0.13) 2.08(0.15) 0.42(>0.05) 0.83(>0.05)
P2 4.13(0.04) 2.37(0.12) 2.95(0.09) 0.47(<0.05) 0.87(<0.05)
RIL 0.24(0.63) 0.03(0.86) 1.43(0.23) 0.15(>0.05) 0(>0.05)
Ⅲ F-2 P1 5.19(0.02) 2.48(0.12) 6.36(0.01) 0.59(<0.05) 0.95(<0.05)
P2 5.66(0.02) 2.50(0.11) 8.38(0.00) 0.63(<0.05) 0.98(<0.05)
RIL 0.50(0.48) 0.01(0.94) 6.06(0.01) 0.21(>0.05) 0(>0.05)
G-0 P1 0(1) 0.05(0.82) 0.80(0.37) 0.07(>0.05) 0.36(>0.05)
P2 0(1) 0.01(0.93) 0.11(0.74) 0.05(>0.05) 0.32(>0.05)
RIL 0.07(0.79) 0.10(0.75) 0.05(0.82) 0.04(>0.05) 0(>0.05)

Table 7

The estimates of genetic parameters of the plant height"

一阶参数
1st order parameter		 估计值Estimate 二阶参数
2nd order parameter		 估计值Estimate
Ⅰ Ⅱ Ⅲ Ⅰ Ⅱ Ⅲ
m 154.89 297.89 362.17 σ2p 661.85 845.25 1 152.12
da 18.21 -20.61 -24.48 σ2mg 410.08 487.98 688.39
db -19.28 -20.58 -24.45 σ2pg - 356.09 451.52
dc -19.28 -20.60 -24.48 σ2e 251.77 1.18 12.21
iab - -20.57 -24.44 h2mg(%) 61.96 57.73 59.75
iac - -20.58 -24.44 h2pg(%) - 42.13 39.19
ibc - -20.56 -24.43

Table 8

The estimates of genetic parameters of the ear height"

一阶参数
1st order parameter		 估计值Estimate 二阶参数
2nd order parameter		 估计值Estimate
Ⅰ Ⅱ Ⅲ Ⅰ Ⅱ Ⅲ
m 54.85 51.23 121.97 σ2p 163.14 171.72 300.10
da 7.32 6.95 -8.33 σ2mg 109.99 114.40 111.55
db -7.24 -7.45 -8.31 σ2pg - - 185.82
dc -7.24 -7.45 -8.32 σ2e 53.15 57.32 2.73
iab - - -8.30 h2mg(%) 67.42 66.62 37.17
iac - - -8.30 h2pg(%) - - 61.92
ibc - - -8.30
iabc - - -8.29
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