Crops ›› 2021, Vol. 37 ›› Issue (1): 32-37.doi: 10.16035/j.issn.1001-7283.2021.01.005

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Allelic Variations of Lipoxygenase (LOX) Activity Genes on Chromosome 4B and Distributions in Different Wheat Regions of China

Wang Liming1,2(), Kong Weiwei1(), Gao Huali1,3, Dong Puhui1, Yan Xuefang1, Wang Chunping1, Wang Honggang2, Li Xingfeng2()   

  1. 1Agronomy College, Henan University of Science and Technology, Luoyang 471003, Henan, China
    2Agronomy College, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai'an 271018, Shandong, China
    3Huangfanqu Farm in Henan Province, Zhoukou 466000, Henan, China
  • Received:2020-04-14 Revised:2020-05-14 Online:2021-02-15 Published:2021-02-23
  • Contact: Li Xingfeng E-mail:lmwang1@163.com;1203879469@qq.com;xfli@sdau.edu.cn

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Abstract:

In order to identify distributions of genes for the lipoxygenase (LOX) activity of grains in different wheat regions in China, 436 wheat cultivars (lines) from seven wheat regions were genotyped by the functional markers LOX16 and LOX18 on the chromosome of 4B. The results showed that: TaLox-B1 had three allelic variations detected by the LOX16 and LOX18: TaLox-B1a (related to higher LOX activity), TaLox-B1b (related to lower LOX activity) and heterozygous type (related to intermediate type, moderate LOX activity), with frequencies of 19.0%, 70.4% and 10.6%, respectively. There were significant differences in the distribution of LOX activity genes among genotypes in different wheat regions. Wheat germplasms with the genotype TaLox-B1a were mostly distributed in the Huang-huai winter wheat region (HHR), Northern winter wheat region (NR) and Yangtze River middle-lower reaches winter wheat region (YMR), with the ratios of 21.1%, 19.8% and 17.6%, respectively. Wheat germplasms with the genotype of TaLox-B1b were mostly distributed in the Southwestern winter wheat region (SWR) and YMR, with the ratios of 87.9% and 72.5%, respectively. Heterozygous type was only found in NR, HHR and YMR, with the ratios of 14.2%, 12.4% and 9.8%, respectively. The markers LOX16 and LOX18 were further used to test LOX genotypes of 53 lines from our breeding program. The results showed that there were only two genotypes of TaLox-B1b and heterozygous type in 53 lines. Among them, 32 lines with heterozygous type in a proportion of 60.4%. Molecular marker-assisted selection was beneficial to the genetic improvement of LOX activity and breeding new wheat varieties.

Key words: Common wheat, LOX, Wheat region, Functional marker, MAS

Table 1

Wheat cultivars (lines) from different wheat regions of China"

代码
Code		 麦区
Wheat region		 品种（系）数
Wheat collection
Ⅰ 黄淮冬麦区 209
Ⅱ 北部冬麦区 106
Ⅲ 西南冬麦区 58
Ⅳ 长江中下游冬麦区 51
Ⅴ 青藏春冬麦区 3
Ⅵ 东北春麦区 4
Ⅶ 西北春麦区 5
自选Self-selection 河南洛阳 53

Table 2

Functional markers and primer sequences of LOX gene on wheat 4B chromosome"

标记Marker 引物序列(5′-3′) Primer sequence (5′-3′) 扩增片段Amplified fragment (bp) 目标等位变异Targeted allele
LOX16 F: CCATGACCTGATCCTTCCCTT 489 TaLox-B1a
R: GCGCGGATAGGGGTGGT
LOX18 F: ACGATGTGAGTTGTGACTTGTG 791 TaLox-B1b
R: GCGCGGATAGGGGTGC

Fig.1

Allelic variation of wheat cultivars (lines) from different wheat regions tested by the marker LOX16 for TaLox-B1 M: DL2000 marker; 1: Zhongguochun; 2: Xiaoyan 22; 3: Xinong 126; 4: Xinong 177; 5: Xiaoyan 22-3; 6: Xinong 291; 7: Xiaoyan 135A; 8:Xiaoyan 81; 9:Xinong 88; 10:Xinong 2000; 11:Xinong B22; 12:Xinong 2611"

Fig.2

Allelic variation of wheat cultivars (lines) from different wheat regions tested by the marker LOX18 for TaLox-B1 M: DL2000 marker; 1: Zhongguochun; 2: Jinan 17; 3: Jimai 19; 4: Jimai 20; 5: Jimai 21; 6: Jimai 22; 7: Lunxuan 987; 8:Tainong 142; 9:Taishan 21; 10:Taishan 23; 11:Shannong 267; 12:Weimai 8"

Table 3

Molecular detection of alleles of wheat TaLox-B1 in different wheat regions"

基因型Genotype 项目Item 麦区Wheat region 品种（系）数
No. of cultivars (lines)		 频率
Frequency (%)
Ⅰ Ⅱ Ⅲ Ⅳ Ⅴ Ⅵ Ⅶ
TaLox-B1a 品种（系）数 44 21 7 9 0 2 0 83
频率Frequency (%) 21.1 19.8 12.1 17.6 0.0 50.0 0.0 19.0
TaLox-B1b 品种（系）数 139 70 51 37 3 2 5 307
频率Frequency (%) 66.5 66.0 87.9 72.5 100.0 50.0 100.0 70.4
杂合型 Heterozygous type 品种（系）数 26 15 0 5 0 0 0 46
频率Frequency (%) 12.4 14.2 0.0 9.8 0.0 0.0 0.0 10.6
合计Total 品种（系）数 209 106 58 51 3 4 5 436
频率Frequency (%) 100.0 100.0 100.0 100.0 100.0 100.0 100.0 100.0
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