Crops ›› 2021, Vol. 37 ›› Issue (1): 32-37.doi: 10.16035/j.issn.1001-7283.2021.01.005

Previous Articles     Next Articles

Allelic Variations of Lipoxygenase (LOX) Activity Genes on Chromosome 4B and Distributions in Different Wheat Regions of China

Wang Liming1,2(), Kong Weiwei1(), Gao Huali1,3, Dong Puhui1, Yan Xuefang1, Wang Chunping1, Wang Honggang2, Li Xingfeng2()   

  1. 1Agronomy College, Henan University of Science and Technology, Luoyang 471003, Henan, China
    2Agronomy College, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai'an 271018, Shandong, China
    3Huangfanqu Farm in Henan Province, Zhoukou 466000, Henan, China
  • Received:2020-04-14 Revised:2020-05-14 Online:2021-02-15 Published:2021-02-23
  • Contact: Li Xingfeng E-mail:lmwang1@163.com;1203879469@qq.com;xfli@sdau.edu.cn

RichHTML

7

PDF (PC)

179

Abstract:

In order to identify distributions of genes for the lipoxygenase (LOX) activity of grains in different wheat regions in China, 436 wheat cultivars (lines) from seven wheat regions were genotyped by the functional markers LOX16 and LOX18 on the chromosome of 4B. The results showed that: TaLox-B1 had three allelic variations detected by the LOX16 and LOX18: TaLox-B1a (related to higher LOX activity), TaLox-B1b (related to lower LOX activity) and heterozygous type (related to intermediate type, moderate LOX activity), with frequencies of 19.0%, 70.4% and 10.6%, respectively. There were significant differences in the distribution of LOX activity genes among genotypes in different wheat regions. Wheat germplasms with the genotype TaLox-B1a were mostly distributed in the Huang-huai winter wheat region (HHR), Northern winter wheat region (NR) and Yangtze River middle-lower reaches winter wheat region (YMR), with the ratios of 21.1%, 19.8% and 17.6%, respectively. Wheat germplasms with the genotype of TaLox-B1b were mostly distributed in the Southwestern winter wheat region (SWR) and YMR, with the ratios of 87.9% and 72.5%, respectively. Heterozygous type was only found in NR, HHR and YMR, with the ratios of 14.2%, 12.4% and 9.8%, respectively. The markers LOX16 and LOX18 were further used to test LOX genotypes of 53 lines from our breeding program. The results showed that there were only two genotypes of TaLox-B1b and heterozygous type in 53 lines. Among them, 32 lines with heterozygous type in a proportion of 60.4%. Molecular marker-assisted selection was beneficial to the genetic improvement of LOX activity and breeding new wheat varieties.

Key words: Common wheat, LOX, Wheat region, Functional marker, MAS

Table 1

Wheat cultivars (lines) from different wheat regions of China"

代码
Code		 麦区
Wheat region		 品种（系）数
Wheat collection
Ⅰ 黄淮冬麦区 209
Ⅱ 北部冬麦区 106
Ⅲ 西南冬麦区 58
Ⅳ 长江中下游冬麦区 51
Ⅴ 青藏春冬麦区 3
Ⅵ 东北春麦区 4
Ⅶ 西北春麦区 5
自选Self-selection 河南洛阳 53

Table 2

Functional markers and primer sequences of LOX gene on wheat 4B chromosome"

标记Marker 引物序列(5′-3′) Primer sequence (5′-3′) 扩增片段Amplified fragment (bp) 目标等位变异Targeted allele
LOX16 F: CCATGACCTGATCCTTCCCTT 489 TaLox-B1a
R: GCGCGGATAGGGGTGGT
LOX18 F: ACGATGTGAGTTGTGACTTGTG 791 TaLox-B1b
R: GCGCGGATAGGGGTGC

Fig.1

Allelic variation of wheat cultivars (lines) from different wheat regions tested by the marker LOX16 for TaLox-B1 M: DL2000 marker; 1: Zhongguochun; 2: Xiaoyan 22; 3: Xinong 126; 4: Xinong 177; 5: Xiaoyan 22-3; 6: Xinong 291; 7: Xiaoyan 135A; 8:Xiaoyan 81; 9:Xinong 88; 10:Xinong 2000; 11:Xinong B22; 12:Xinong 2611"

Fig.2

Allelic variation of wheat cultivars (lines) from different wheat regions tested by the marker LOX18 for TaLox-B1 M: DL2000 marker; 1: Zhongguochun; 2: Jinan 17; 3: Jimai 19; 4: Jimai 20; 5: Jimai 21; 6: Jimai 22; 7: Lunxuan 987; 8:Tainong 142; 9:Taishan 21; 10:Taishan 23; 11:Shannong 267; 12:Weimai 8"

Table 3

Molecular detection of alleles of wheat TaLox-B1 in different wheat regions"

基因型Genotype 项目Item 麦区Wheat region 品种（系）数
No. of cultivars (lines)		 频率
Frequency (%)
Ⅰ Ⅱ Ⅲ Ⅳ Ⅴ Ⅵ Ⅶ
TaLox-B1a 品种（系）数 44 21 7 9 0 2 0 83
频率Frequency (%) 21.1 19.8 12.1 17.6 0.0 50.0 0.0 19.0
TaLox-B1b 品种（系）数 139 70 51 37 3 2 5 307
频率Frequency (%) 66.5 66.0 87.9 72.5 100.0 50.0 100.0 70.4
杂合型 Heterozygous type 品种（系）数 26 15 0 5 0 0 0 46
频率Frequency (%) 12.4 14.2 0.0 9.8 0.0 0.0 0.0 10.6
合计Total 品种（系）数 209 106 58 51 3 4 5 436
频率Frequency (%) 100.0 100.0 100.0 100.0 100.0 100.0 100.0 100.0
[1] 郑文寅. 小麦籽粒LOX活性的遗传变异和STS标记开发. 合肥:安徽农业大学, 2014.
[2] Siedow J N. Plant lipoxygenase,structure and function. Annual Review of Plant Physiology and Plant Molecular Biology, 1991,42(1):145-188.
doi: 10.1146/annurev.pp.42.060191.001045
[3] Rosahl S. Lipoxygenases in plants -their role in development and stress response. Zeitschrift für Naturforschung C-A. Journal of Biosciences, 1996,51(3/4):123-138.
[4] Brash A R. Lipoxygenases, occurrence,functions,catalysis,and acquisition of substrate. Journal of Biological Chemistry, 1999,274(34):23679-23682.
doi: 10.1074/jbc.274.34.23679
[5] 牟艺菲. 小麦OPR和LOX基因家族的鉴定及其抗逆功能分析. 杨凌:西北农林科技大学, 2019.
[6] Feussner I, Wasternack C. The Lipoxygenase pathway. Annual Review of Plant Biology, 2002,53(1):275-297.
[7] Mosblech A, Feussner I, Heilmann I. Oxylipins,structurally diverse metabolites from fatty acid oxidation. Plant Physiology and Biochemistry, 2009,47(6):511-517.
pmid: 19167233
[8] 郑文寅, 汪帆, 司红起, 等. 普通小麦籽粒LOX、PPO活性和类胡萝卜素含量变异及对全麦粉色泽的影响. 中国农业科学, 2013,46(6):1087-1094.
doi: 10.3864/j.issn.0578-1752.2013.06.001
[9] Geng H W, Xia X C, Zhang L P, et al. Development of functional markers for alipoxygenase gene TaLox-B1 on chromosome 4BS in common wheat. Crop Science, 2012,52(2):568-576.
doi: 10.2135/cropsci2011.07.0365
[10] 吴萍. 小麦籽粒脂肪氧化酶活性功能标记的开发与应用. 合肥:安徽农业大学, 2013.
[11] Leenhardt F, Lyan B, Rock E, et al. Genetic variability of carotenoid concentration,and lipoxygenase and peroxidase activities among cultivated wheat species and bread wheat varieties. European Journal of Agronomy, 2006,25(2):170-176.
doi: 10.1016/j.eja.2006.04.010
[12] Irvine G N, Winkler C A, Anderson J A. Factors affecting the color of macaroni. III. Varietal differences in the rate of pigment destruction during mixing. Cereal Chemistry, 1950,27:205-218.
[13] 李浪. 小麦面粉品质改良与检测技术. 北京: 化学工业出版社, 2008.
[14] 孙道杰, 何中虎, 王辉. 小麦面粉黄色素相关基因研究. 西北植物学报, 2006,26(4):7-12.
[15] Borrelli G M, Troccoli A, Di Fonzo N, et al. Durum wheat lipoxygenase activity and other quality parameters that affect pasta color. Cereal Chemistry, 1999,76(3):335-340.
doi: 10.1094/CCHEM.1999.76.3.335
[16] Borrelli G M, De Leonardis A M, Fares C, et al. Effects of modified processing conditions on oxidative properties of semolina dough and pasta. Cereal Chemistry, 2003,80(2):225-231.
doi: 10.1094/CCHEM.2003.80.2.225
[17] Bao X T, Zhuansun X X, Zheng W Y, et al. Lipoxygenase activity and its relationship with traits of whole-mill color in wheat. Agricultural Science and Technology, 2016,17(10):2242-2246,2255.
[18] 王慧, 郑文寅, 樊宏, 等. 不同小麦品种籽粒中LOX活性及基因型和环境互作分析. 中国粮油学报, 2011,26(1):11-14,19.
[19] Trufanov V A, Permyakova M D, Pshenichnikova T A, et al. The effect of intercultivar substitution of wheat Triticum aestivum L. chromosomes on lipoxygenase activity and its correlation with the technological properties of flour. Applied Biochemistryand Microbiology, 2007,43(1):91-97.
[20] Yonekura L, Nagao A. Intestinal absorption of dietary carotenoids. Molecular Nutrition and Food Research, 2007,51(1):107-115
doi: 10.1002/mnfr.200600145 pmid: 17195263
[21] Tourniaire F, Gouranton E, von Lintig J, et al. β-Carotene conversion products and their effects on adipose tissue. Genes and Nutrition, 2009,4:179-187.
doi: 10.1007/s12263-009-0128-3 pmid: 19557453
[22] Ziegler R G. A Review of epidemiologic evidence that carotenoids reduce the risk of cancer. The Journal of Nutrition, 1989,119(1):116-122
doi: 10.1093/jn/119.1.116 pmid: 2643694
[23] Howitt C A, Pogson B J. Carotenoid accumulation and function in seeds and non-green tissues. Plant Cell and Environment, 2006,29(3):435-445.
doi: 10.1111/pce.2006.29.issue-3
[24] Carpentier S, Knaus M, Suh M. Associations between lutein,zeaxanthin,and age-related macular degeneration,an overview. Critical Reviews in Food ence and Nutrition, 2009,49(4):313-326.
[25] Liu X L, Li B B, Guo L J, et al. Analysis of seed storage-tolerance of wheat (Triticum aestivum) lipoxygenase RNAi transgenic lines. Journal of Agricultural Biotechnology, 2016,24(7):987-996.
[26] Geng H W, Zhang Y, He Z H, et al. Molecular markers for tracking variation in lipoxygenase activity in wheat breeding. Molecular Breeding, 2011,28(1):117-126.
doi: 10.1007/s11032-010-9466-5
[27] Feng B, Dong Z Y, Xu Z B, et al. Molecular analysis of lipoxygenase (LOX) genes in common wheat and phylogenetic investigation of LOX proteins from model and crop plants. Journal of Cereal Science, 2010,52(3):387-394.
doi: 10.1016/j.jcs.2010.06.019
[28] Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 1980,8(19):4321.
doi: 10.1093/nar/8.19.4321 pmid: 7433111
[29] 王黎明, 李永霞, 高华利, 等. 小麦穗发芽活性基因等位变异及其区域分布研究. 西北植物学报, 2019,39(1):52-58.
[30] 相吉山, 穆培源, 桑伟, 等. 新疆小麦品种资源脂肪氧化酶活性基因TaLox-B1的分布特征研究. 麦类作物学报, 2013,33(2):279-285.
doi: 10.7606/j.issn.1009-1041.2013.02.012
[31] 张钰玉, 王晓龙, 张晓科, 等. 陕西小麦品种(系)脂肪氧化酶活性基因的遗传多样性分析. 麦类作物学报, 2012,32(4):616-621.
doi: 10.7606/j.issn.1009-1041.2012.04.004
[32] 曹东, 张雪婷, 王世红, 等. 104份甘肃小麦品种脂肪氧化酶和多酚氧化酶活性基因等位变异的检测. 麦类作物学报, 2014,34(4):467-473.
[33] Bai L, Yu F X, Yaseng R, et al. Molecular identification and distribution of TaLox-B1,TaLox-B2 and TaLox-B3 loci in Xinjiang wheat cultivars. Journal of Triticeae Crops, 2016,36(11):1456-1463.
[34] 张福彦, 陈锋, 程仲杰, 等. 小麦TaLox-B等位变异对脂肪氧化酶活性和面粉色泽的影响. 中国农业科学, 2017,50(8):1370-1377.
doi: 10.3864/j.issn.0578-1752.2017.08.002
[1] Zhao Yuyang, Song Jian, Qiu Lijuan. Proteomic Comparation Analysis of Thylakoid in Leaves of G-Locus Near Isogenic Line in Soybean [J]. Crops, 2020, 36(6): 8-16.
[2] Tian Mengxiang,Gong Yanlong,Zhang Shilong,He Youxun,Lei Yue,Yu Benxun,Yu Li,Li Jiali,Zhang Dashuang,Ye Yongyin. Design and Verification of New Functional Marker of Chilling-Tolerance COLD1 Gene in Rice Seedling Stage [J]. Crops, 2020, 36(1): 55-60.
[3] Chen Tingmu,Sun Zhiguang,Xing Yungao,Fang Zhaowei,Wang Baoxiang,Liu Yan,Xu Dayong. Study on the Method of Determining Digestible Protein Content and Screening of Rice Resources [J]. Crops, 2020, 36(1): 61-66.
[4] Shi Zhaokang,Zhao Zequn,Zhang Yuanhang,Xu Shiying,Wang Ning,Wang Weijie,Cheng Hao,Xing Guofang,Feng Wanjun. The Response and Cluster Analysis of Biomass Accumulation and Root Morphology of Maize Inbred Lines Seedlings to Two Nitrogen Application Levels [J]. Crops, 2019, 35(5): 28-36.
[5] Narong Shi,Jingjing Li,Huiyu Wu,Daojie Sun,Yi Feng,Hui Wang,Xinlun Liu,Lingli Zhang. Genetic Relationship of Xinong 979 and Thinopyrum ponticum Based on Pedigree Analysis and Molecular Markers [J]. Crops, 2019, 35(1): 15-21.
[6] Wang Xiaolin,Ji Xiaoling,Zhang Panpan,Zhang Xiong,Zhang Jing. Correlation Analysis between Aboveground Biomass Allocation and Grain Yield in Different Varieties of Foxtail Millet in the Dry Land of Loess Plateau [J]. Crops, 2018, 34(5): 150-155.
[7] Lidan Pang,Tingting Meng,Yufei Zhang,Yuxuan Ma,Sen Zhou,Fulin Wang,Jianjun Dai. Effects of Maize Straw Returning with Nitrogen Fertilizer on Soil Enzyme Activity,Microbial Biomass Carbon Content and Respiration [J]. Crops, 2017, 33(1): 107-112.
[8] Min Hu,Jianwei Lu,Zhen Wang,Qiuxiang You. Study on the Appropriate Seeding Rate of Late Sowing Oilseed Rape as Green Manure [J]. Crops, 2016, 32(6): 120-123.
[9] Jinjun Cai,Wei Dang,Liguo Dong,Yuanrun Zhang,Yueling Wang. Mixed Sowing Technology between Alfalfa and Leymus chinensis in the Mountainous Area of Southern Ningxia [J]. Crops, 2016, 32(3): 123-127.
[10] . [J]. Crops, 2013, 29(3): 67-71.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[2] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[3] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[4] Fei Yang,Wenli Ma,Yongwei Chen,Zhansheng Zhang,Hao Wang. The Effects of Uniform Sowing and Drip Irrigation on the Spike Differentiation and Yield of Spring Wheat[J]. Crops, 2018, 34(4): 84 -88 .
[5] Shanshan Lu,Chenglai Wu,Yan Li,Chunqing Zhang. The Molecular Basis of Holding the Feature and Genetic Purity for Maize Inbred Lines[J]. Crops, 2018, 34(1): 41 -48 .
[6] Yue Jiao,Wei Fu,Yong Zhai. Application of RNAi in Crop Breeding and Its Safety Assessment[J]. Crops, 2018, 34(1): 9 -15 .
[7] . [J]. Crops, 2009, 25(6): 13 -17 .
[8] . [J]. Crops, 2003, 19(3): 33 -34 .
[9] . [J]. Crops, 2012, 28(2): 10 -15 .
[10] . [J]. Crops, 1993, 9(2): 33 -34 .