Crops ›› 2022, Vol. 38 ›› Issue (2): 81-88.doi: 10.16035/j.issn.1001-7283.2022.02.012

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The Role of Wheat Deplantation-Related Genes in Degradation of Chlorophyll in Spring Wheat Leaves

Zhao Kai(), Jin Xiujuan, Sun Lili, Yan Rongyue, Lu Juan, Guo Feng, Md Ashraful Islam, Shi Yugang, Sun Daizhen()   

  1. College of Agricultural, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China
  • Received:2021-10-14 Revised:2021-12-30 Online:2022-04-15 Published:2022-04-24
  • Contact: Sun Daizhen E-mail:zk51712@163.com;sdz64@126.com

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Abstract:

Leaf senescence of wheat leads to yield loss, and chlorophyll degradation is an important feature of wheat leaf senescence. The purpose of this study is to explore the role of chlorophyll degradation-related genes chlorophyllase (TaCLH) and pheophytin pheophorbide hydrolase (TaPPH) in the senescence process of spring wheat leaves, and to provide a reference for analyzing the molecular mechanism of chlorophyll degradation in wheat. Ten spring wheats were used as test materials. The relative expression of TaCLH and TaPPH in the flag leaves during the aging process were measured, the relative chlorophyll content (SPAD value) and functional leaf area (GLAD) of different wheat varieties at different stages after anthesis as well as the changes in chlorophyll fluorescent parameters were analyzed to explore the correlation between TaCLH and TaPPH with SPAD, GLAD and chlorophyll fluorescent parameters during leaf senescence. The results showed that there were extremely significant negative correlations between TaPPH relative expression level with SPAD, GLAD and chlorophyll fluorescence parameters [ETR, Fv/Fm, Y(II)], and there was a highly significant positive correlation between the relative expression levels of TaCLH and TaPPH. This indicated that TaPPH played a major regulatory role in the flag leaf senescence process after anthesis in spring wheat.

Key words: Wheat, Chlorophyll, Fluorescence parameters, TaCLH, TaPPH, Gene expression

Fig. 1

Dynamic changes of SPAD and GLAD values of flag leaves on main stem of different spring wheat varieties after anthesis"

Table 1

Aging characteristic parameters based on SPAD and GLAD values in different spring wheats after anthesis"

指标
Index		 品种
Variety		 特征参数Characteristic parameter
MRS TMRS (d) Ts (d) T0 (d)
SPAD 晋麦2148 5.48ab 23.97ef 15.60ab 27.07d
宁春13号 4.38bc 24.90cdef 14.21ab 28.88cd
内麦19号 3.62cd 23.72f 11.79bc 28.22cd
博爱7023 5.02bcd 26.01bc 16.56a 29.51abc
生选3号 5.39ab 25.46cd 16.78a 28.67bcd
川麦22 6.72a 27.31a 19.11a 30.34a
绵阳15号 4.51bcd 27.10ab 17.17a 30.77a
新春8号 3.93cd 25.56cd 12.54bc 30.48a
宁春4号 3.58cd 24.59def 11.08c 29.77abc
青春5号 3.44d 25.22cde 12.72bc 29.92ab
GLAD值
GLAD value		 晋麦2148 0.74a 27.28abc 15.78a 31.55abc
宁春13号 0.75a 28.16a 16.68a 32.42ab
内麦19号 0.80a 27.16abc 16.58a 31.09abc
博爱7023 0.85a 24.57ef 14.59a 28.27bc
生选3号 0.76a 23.94f 13.15a 27.97c
川麦22 0.66a 27.29ab 13.89a 32.32a
绵阳15号 0.60a 27.18ab 13.69a 32.25a
新春8号 0.70a 26.33bcd 13.94a 30.97abc
宁春4号 0.62a 25.73cde 12.13a 30.90abc
青春5号 0.65a 25.59de 12.29a 30.63abc

Fig.2

The systematic clustering diagram of different spring wheat based on SPAD values"

Table 2

Aging characteristic parameters based on SPAD and GLAD values in three groups of wheat"

指标
Index		 类群
Group		 特征参数Characteristic parameter
MRS TMRS (d) Ts (d) T0 (d)
SPAD Ⅰ 5.42 25.97 17.04 29.27
Ⅱ 3.64 24.77 12.03 29.60
Ⅲ 4.38 24.90 14.21 28.88
GLAD值
GLAD value		 Ⅰ 0.79 26.79 15.91 30.83
Ⅱ 0.73 25.14 13.55 29.47
Ⅲ 0.63 26.45 13.00 31.53

Fig.3

The systematic clustering diagram of different spring wheat based on GLAD values"

Fig.4

Dynamic changes of chlorophyll fluorescence parameters of flag leaves on main stem of different spring wheat varieties after anthesis"

Fig.5

Dynamic changes of chlorophyll fluorescence parameters of flag leaves in different postanthesis stages of two types of wheats"

Fig.6

Dynamic changes of TaCLH and TaPPH gene relative expression level of flag leaves on main stem of different spring wheat varieties after postanthesis"

Table 3

Correlation coefficients of traits"

指标Index Y(II) Fv/Fm ETR qP GLAD值GLAD value SPAD TaCLH TaPPH
Y(II) 1
Fv/Fm 0.952** 1
ETR 0.919** 0.928** 1
qP 0.063 0.014 0.083 1
GLAD 0.919** 0.914** 0.840** 0.054 1
SPAD 0.913** 0.945** 0.893** 0.010 0.905** 1
TaCLH -0.075 -0.076 -0.006 0.100 -0.061 -0.089 1
TaPPH -0.587** -0.564** -0.450** 0.121 -0.591** -0.575** 0.391** 1
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