Crops ›› 2022, Vol. 38 ›› Issue (3): 104-108.doi: 10.16035/j.issn.1001-7283.2022.03.015

Previous Articles     Next Articles

Bioinformatics and Expression Analysis of HaLACS7 Gene in Sunflower

Zhou Fei1,2()   

  1. 1Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin 150086, Heilongjiang, China
    2Industrial Crops Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
  • Received:2021-04-12 Revised:2021-07-01 Online:2022-06-15 Published:2022-06-20

RichHTML

3

PDF (PC)

250

Abstract:

LACS encodes for long-chain acyl-CoA synthetase, a protein that converts free fatty acids into long-chain acyl-CoA, an essential lipid metabolite. The bioinformatics analysis of the HaLACS7 encoded protein in sunflower was carried out in this study. The protein encoded by HaLACS7 was expected to be an acidic and hydrophilic protein. The protein encoded by HaLACS7 had an irregular coil secondary structure, and it’s subcellular location was anticipated to be in the peroxisome. With 84% sequence similarity, HaLACS7 was shown to be closely related to Lactuca sativa L. and Cynara cardunculus var. Scolymus L. LACS6, and clustered in a broad branch with LACS6 and LACS7 of Arabidopsis thaliana L. The expression level of HaLACS7 in sunflower tissues was ligulate flowers > root > seed > tubular flower > leaf > stem. The expression level of HaLACS7 was higher at the late development stage of high and low oleic acid sunflower seeds, but the expression trend was significantly different at different seed development stages of the two materials. Therefore, it was suggested that the expression level of HaLACS7 may affect the synthesis of oleic acid in sunflower seeds.

Key words: Sunflower, Oleic acid, HaLACS7, Bioinformatics, Expression anaylsis

Table 1

Amino acid composition of protein encoded by HaLACS7 in sunflower"

种类Category 数量Quantity 质量分数Mass fraction (%) 种类Category 数量Quantity 质量分数Mass fraction (%)
丙氨酸Ala (A) 18 5.7 甲硫氨酸Met (M) 7 2.2
精氨酸Arg (R) 14 4.4 苯丙氨酸Phe (F) 18 5.7
天冬酰胺Asn (N) 17 5.4 脯氨酸Pro (P) 18 5.7
天冬氨酸Asp (D) 17 5.4 丝氨酸Ser (S) 21 6.7
半胱氨酸Cys (C) 10 3.2 苏氨酸Thr (T) 12 3.8
谷氨酰胺Gln (Q) 12 3.8 色氨酸Trp (W) 6 1.9
谷氨酸Glu (E) 20 6.3 酪氨酸Tyr (Y) 12 3.8
甘氨酸Gly (G) 19 6.0 缬氨酸Val (V) 27 8.6
组氨酸His (H) 5 1.6 吡咯赖氨酸Pyl (O) 0 0.0
异亮氨酸Ile (I) 24 7.6 赖氨酸Lys (K) 19 6.0
亮氨酸Leu (L) 19 6.0 硒代半胱氨酸Sec (U) 0 0.0

Fig.1

Secondary structure prediction of protein encoded by HaLACS7 in sunflower"

Fig.2

Hydrophilic/hydrophobic analysis of protein encoded by HaLACS7 in sunflower"

Fig.3

Phylogenetic tree analysis of HaLACS7 gene in sunflower"

Fig.4

The expression patterns of HaLACS7 gene at different development stages and different parts"

[1] Jessen D, Roth C, Wiermer M, et al. Two activities of long-chain acyl-coenzyme A synthetase are involved in lipid trafficking between the endoplasmic reticulum and the plastid in Arabidopsis. Plant Physiology, 2015, 167(2):351-366.
doi: 10.1104/pp.114.250365 pmid: 25540329
[2] Koo A J K, Ohlrogge J B, Pollard M. On the export of fatty acids from the chloroplast. Journal of Biological Chemistry, 2004, 279(16):16101-16110.
doi: 10.1074/jbc.M311305200
[3] 汪信东, 江香梅, 章挺, 等. 樟树长链脂肪酰CoA合成酶1鉴定与功能初步分析. 分子植物育种, 2018, 16(16):105-114.
[4] Ding L N, Gu S L, Zhu F G, et al. Long-chain acyl-CoA synthetase 2 is involved in seed oil production in Brassica napus. BMC Plant Biology, 2020, 20(1):21-34.
doi: 10.1186/s12870-020-2240-x
[5] Aznar-Moreno J A. Calerón M V, Martínez-Force E, et al. Sunflower (Helianthus annuus) long-chain acyl-coenzyme A synthetases expressed at high levels in developing seeds. Physiologia Plantarum, 2014, 150(3):363-373.
doi: 10.1111/ppl.12107
[6] Zhao L F, Haslam T M, Sonntag A, et al. Functional overlap of long-chain acyl-CoA synthetases in Arabidopsis. Plant and Cell Physiology, 2019, 60(5):1041-1054.
doi: 10.1093/pcp/pcz019
[7] Fulda M, Shockey J, Werber M, et al. Two long-chain acyl-CoA synthetases from Arabidopsis thaliana involved in peroxisomal fatty acid β-oxidation. The Plant Journal, 2002, 32(1):93-103.
doi: 10.1046/j.1365-313X.2002.01405.x
[8] 徐靖, 李辉亮, 朱家红, 等. 植物脂肪酸β-氧化的研究进展. 生物技术通讯, 2008, 19(1):141-144.
[9] Shockey J M, Fulda M, Browse J A. Arabidopsis contains nine long-chain acyl-coenzyme a synthetase genes that participate in fatty acid and glycerolipid metabolism. Plant Physiology, 2002, 129(4):1710-1722.
pmid: 12177484
[10] 江南, 谭晓风, 张琳, 等. 油茶长链脂肪酰基CoA合成酶基因1(CoLACS1) 分子特征与表达分析. 植物遗传资源学报, 2017, 18(1):139-147.
[11] 徐日荣, 陈湘瑜, 唐兆秀. 花生长链酰基辅酶A合成酶6基因(LACS6)的克隆、鉴定与组织表达. 福建农业学报, 2016, 31(11):1164-1170.
[12] 汪信东, 章挺, 杨海宽, 等. 樟树长链脂肪酰基CoA合成酶基因9克隆与表达分析. 广西植物, 2018, 38(10):1335-1345.
[13] Xu Y, Holic R, Li D, et al. Substrate preferences of long-chain acyl-CoA synthetase and diacylglycerol acyltransferase contribute to enrichment of flax seed oil with а-linolenic acid. Biochemical Journal, 2018, 475(8):1473-1489.
doi: 10.1042/BCJ20170910
[1] Jia Xiuping, Mao Xuhui, Liang Gensheng, Liu Runping, Liu Feng, Wang Xingzhen. Analysis of Physiological and Biochemical Mechanism and Growth and Development Characteristics of Saline and Alkali Resistance in Sunflower [J]. Crops, 2022, 38(5): 146-152.
[2] Shi Bixian, Tao Jianfei, Gao Yan, Xie Huihong, Abulimiti·Aierken , Cheng Pingshan, Maitituersun·Sadike , Sha Hong. Effects of Different Planting Densities on the Morphological Traits and Yields of Three Confectionery Sunflower Varieties [J]. Crops, 2022, 38(5): 195-200.
[3] Ling Yibo, Feng Yunge, Wang Binjie, Zhang Kai, Chen Nianlai. Effects of Density and Row Spacing on Canopy Structure and Photosynthetic Characteristics in Sunflower [J]. Crops, 2022, 38(3): 155-160.
[4] Guo Shuchun, Miao Hongmei, Li Suping, Yu Haifeng, Nie Hui, Mu Yingnan, Wen Xinyu, Liang Chen, Zhang Haibin, Shao Ying. Research Advances in the Breeding Study of Sunflower Resistance to Orobanche [J]. Crops, 2022, 38(3): 27-32.
[5] Yang Xiaolin, Duan Ying, Cai Suyun, He Runli, Yin Guifang, Wang Yanqing, Lu Wenjie, Sun Daowang, Wang Lihua. Molecular Cloning and Bioinformatics Analyzing of Laccase in Fagopyrum tataricum [J]. Crops, 2022, 38(3): 73-79.
[6] Lu Ping, Kang Qingfang, Zhao Mengyao, Zhang Fengjie, Wu Qiangqiang, Ma Fangfang, Wang Yushen, Han Yuanhuai, Wang Xingchun, Li Xueyin. Identification and Functional Analysis of miR169 Family and Its Target Genes in Setaria italica [J]. Crops, 2022, 38(2): 54-63.
[7] Li Yang, Ren Xiaoci, Li Xiaowei, Li Weitang, Huang Wei, He Zhongguo, Wang Baizhong. Combining Ability and Genetic Analysis of Yield and Main Grain Characteristics of Edible Sunflower [J]. Crops, 2022, 38(2): 75-80.
[8] Yin Guifang, Duan Ying, Yang Xiaolin, Cai Suyun, Wang Yanqing, Lu Wenjie, Sun Daowang, He Runli, Wang Lihua. Cloning and Bioinformatics Analysis of FtC4H Gene from Tartary Buckwheat [J]. Crops, 2022, 38(1): 77-83.
[9] Zhao Xuanwei, Zhao Yajie, Tian Zhendong, Hu Shuping, Zhao Rong, Ren Yaning, Bao Haizhu, Gao Julin. Response of Dry Matter Transportion and Yield to Sowing Date and Planting Density on Sunflower [J]. Crops, 2021, 37(3): 185-189.
[10] Zhou Fei, Wang Wenjun, Liu Yan, Ma Jun, Wang Jing, Wu Liren, Guan Hongjiang, Huang Xutang. Establishment of Near-Infrared Spectroscopy Model for the Contents of Fat and Fatty Acids in Sunflower Husked Seeds [J]. Crops, 2021, 37(2): 200-206.
[11] Di Na, Zheng Na, Han Haijun, Wang Jing, Cui Chao, Zheng Xiqing. The Stimulatory Effect of Different Sunflower Varieties Root Exudates on Germination of Sunflower Broomrape [J]. Crops, 2020, 36(6): 197-201.
[12] Yang Haifeng, Duan Xueyan, Wei Ling, Liu Bo. The Genetic Study of Yield Traits in Edible Sunflower [J]. Crops, 2020, 36(5): 93-97.
[13] Shan Feibiao, Du Ruixia, Wang Yongxing, Yang Qinfang, Liu Chunhui, Chen Yang. Genetic Diversity Analysis of Sunflower (Helianthus annuus L.) Based on DUS Testing [J]. Crops, 2020, 36(4): 107-113.
[14] Zhang Haiyang, Li Haiyan, Meng Qinglin, He Chaoqun, Liu Shuqing. Effects of Different Fungicides on Field Control of Sunflower Sclerotinia Rot [J]. Crops, 2020, 36(4): 202-205.
[15] Zhou Zichao, Hou Jianhua, Zhen Zilong, Shi Huimin. Drought-Resistance Identification and Evaluation of 152 Sunflower Recombinant Inbred Lines (RILs) at Seedling Stage [J]. Crops, 2020, 36(3): 47-52.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!