Crops ›› 2022, Vol. 38 ›› Issue (4): 167-171.doi: 10.16035/j.issn.1001-7283.2022.04.023

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Response of Tobacco Seedling Growth and Development to Combined Application of Brassinolide and Microbial Agents

Li Diqin1(), Peng Yuanyuan1, Wang Yan1, Li Sijun2, Peng Tianwei1, Wang Qing1, Li Qiang1, Xie Huiya3()   

  1. 1College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, China
    2Chenzhou Company of Hunan Tobacco Company, Chenzhou 423000, Hunan, China
    3Zhuzhou Company of Hunan Tobacco Company, Zhuzhou 412000, Hunan, China
  • Received:2021-04-12 Revised:2022-06-14 Online:2022-08-15 Published:2022-08-22
  • Contact: Xie Huiya E-mail:ldqhnnd2009@163.com;290615790@qq.com

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Abstract:

In order to study the effects of brassinolide and Bacillus subtilis on the growth and development of tobacco seedlings, Xiangyan 5 was used as the material, and different concentrations of brassinolide and Bacillus subtilis agents were sprayed on tobacco seedlings of four leaves-one core. The amount of dry matter accumulation, root activity, chlorophyll levels, and related enzyme activities of eight leaves-one core of tobacco seedlings were measured and analysed. The results demonstrated that the amount of dry matter accumulation, root activity, chlorophyll content, and nitrate reductase activity of tobacco seedlings treated with brassinolide and Bacillus subtilis agents were superior to those of the controls; and the treatment of spraying 0.004% brassinolide with 0.25 or 7.50g Bacillus subtilis agent the dry weight of whole plant (4.59g/plant), root activity [90.31μg/(g·h) FW], the max-leaf chlorophyll content (0.5018mg/g FW) and nitrate reductase activity (0.99U/g FW) were the highest and significantly (P < 0.05) higher than that of the control; and activities of superoxide dismutase activity (56.5U/g FW) and peroxidase (2714.7U/g FW), and the content of malondialdehyde (8.8mg/g FW) were the lowest and significantly (P < 0.05) lower than that of the control. The results showed that the mixture of brassinolide and Bacillus subtilis agents could improve the quality of tobacco seedlings and cultivate strong seedlings.

Key words: Flue cured tobacco, Growth and development, Brassinolide, Bacillus subtilis

Fig.1

Dry weight and ratio of fresh to dry weight of different treatments The different small letters indicate significant difference at the 5% level"

Table 1

Chlorophyll contents of the largest leaf and root vigor of different treatments"

处理
Treatment		 根系活力
Root vigor [μg/(g·h FW)]		 叶绿素a含量
Chlorophyll a content (mg/g FW)		 叶绿素b含量
Chlorophyll b content (mg/g FW)		 叶绿素(a+b)含量
Chlorophyll (a+b) content (mg/g FW)
T1 90.30±0.50a 0.336±0.002a 0.166±0.010a 0.502±0.002a
T2 69.00±0.59d 0.295±0.002b 0.123±0.001c 0.417±0.003c
T3 81.00±0.13b 0.326±0.001a 0.136±0.001b 0.462±0.001b
T4 66.90±0.13e 0.280±0.001bc 0.110±0.001e 0.390±0.001d
T5 74.10±0.15c 0.319±0.001a 0.117±0.002d 0.437±0.002c
T6 64.10±0.17f 0.264±0.001c 0.086±0.001f 0.351±0.001e
CK 60.10±0.17g 0.199±0.034d 0.073±0.001e 0.273±0.034f

Table 2

Enzymes activities and MDA contents in the largest leaf of different treatments"

处理
Treatment		 SOD活性
SOD activity (U/g FW)		 POD活性
POD activity (U/g FW)		 CAT活性
CAT activity (U/g FW)		 NR活性
NR activity (U/g FW)		 MDA含量
MDA content (mg/g FW)
T1 56.5±1.1e 2714.7±40.7e 10.0±0.4f 0.99±0.02a 8.8±0.0g
T2 69.7±0.5c 3110.8±61.5c 32.9±0.2c 0.54±0.01d 12.1±0.0d
T3 59.2±2.0de 2934.7±47.4d 12.6±0.2e 0.86±0.01b 10.4±0.0f
T4 79.4±1.6b 3566.7±50.4b 36.9±0.2b 0.49±0.01e 12.9±0.0c
T5 61.7±1.0d 3039.3±131.2cd 13.7±0.2d 0.76±0.01c 11.4±0.0e
T6 82.0±0.7ab 3680.6±59.4b 36.9±0.3b 0.47±0.01f 13.6±0.1b
CK 84.7±2.6a 4219.6±30.0a 41.0±0.2a 0.43±0.01g 15.9±0.1a

Table 3

Correlation of dry matter content with enzyme activities and MDA content"

部位Position SOD活性
SOD activity		 POD活性
POD activity		 CAT活性
CAT activity		 NR活性
NR activity		 MDA含量
MDA content
根干重Root dry weight -0.9637** -0.9348** -0.9608** 0.9153** -0.9032**
茎干重Stem dry weight -0.8947** -0.8224** -0.7940* 0.8907** -0.9528**
叶干重Leaf dry weight -0.9136** -0.9748** -0.9161** 0.9689** -0.8610**

Table 4

Correlation between the largest leaf chlorophyll content and enzyme activities and MDA content"

指标
Index		 叶绿素a含量
Chlorophyll a
content		 叶绿素b含量
Chlorophyll b
content		 叶绿素(a+b)含量
Chlorophyll
(a+b) content
SOD活性
SOD activity		 0.903** 0.899** 0.925**
POD活性
POD activity		 0.869* 0.932** 0.918**
CAT活性
CAT activity		 0.826* 0.831* 0.851*
NR活性
NR activity		 0.838* 0.892** 0.883**
MDA含量
MDA content		 0.862* 0.956** 0.922**
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