Crops ›› 2024, Vol. 40 ›› Issue (3): 238-246.doi: 10.16035/j.issn.1001-7283.2024.03.032

Previous Articles     Next Articles

Effects of Film Mulching on Rhizosphere Soil Microbial Community of Maize in Wind-Sand Grassy Beach Area of Northern Shaanxi Province

Li Jiaqi(), Shi Jianguo(), Chen Qihang, Chang Fengyun, Duan Yizhong, Chai Guaiqiang, Jia Lei, Chen Tao   

  1. Yulin University / Key Laboratory of Ecological Restoration of Mining Areas in Northern Shaanxi Province, Yulin 719000, Shaanxi, China
  • Received:2023-02-20 Revised:2023-05-06 Online:2024-06-15 Published:2024-06-18

RichHTML

2

PDF (PC)

162

Abstract:

In order to clarify the changes of microbial community structure in maize rhizosphere soil under different mulching conditions, six samples of maize rhizosphere soil under different mulching conditions were collected. High-throughput sequencing technology was used to sequence the gene regions of microbial samples of maize rhizosphere soil, so as to analyze the community structure characteristics of bacteria and fungi in the sample rhizosphere soil, and reveal the influence of mulching soil on the quality of maize rhizosphere soil. The results showed that the total OTU number of bacteria and fungi in the field covered with mulch were higher than that in the field without mulching. Chaol and Shannon indexes revealed that the richness and diversity of fungi and bacteria community in the rhizosphere soil of maize under mulching were higher than those without mulching. At the phylum level, the relative abundance of Proteobacteria and Bacteroidota were decreased and that of Acidobacteria, Gemmatimonadota, Ascomycota and Glomeromycota were increased. At the genus level, film mulching caused the enrichment of MND1, Haliangium, Metagenome, RB41, Sphingomonas in bacteria and Exophiala, Metarhizium, Botryotrichum, Coniochaeta, Lophotrichus, Fusarium in fungi in the rhizosphere soil of maize. This study presented the scientific theoretical foundation for the selection, use, and exploitation of beneficial microorganisms in maize production. It also demonstrated the impact of agricultural mulching on the ecological effect of maize rhizosphere soil.

Key words: Film mulching, Maize rhizosphere soil, High-throughput sequencing, Microbial community

Fig.1

Rarefaction curves of the fungal (a) and bacteria (b) samples"

Fig.2

The number of OTUs in fungal (a) and bacteria (b) samples"

Fig.3

OTU-Venn graph of rhizosphere soil fungi in maize"

Fig.4

OTU-Venn graph of rhizosphere soil bacteria in maize"

Table 1

Diversity indexes of fungus and bacteria in rhizosphere soil of each sample"

项目
Item		 处理
Treatment		 样品
Sample		 Chao1指数
Chao1 index		 观测深度
Observation depth		 观测OTU数
Observed OTU species		 系谱多样指数
PD whole tree		 Shannon指数
Shannon index		 Simpson指数
Simpson index
真菌Fungus M M1 2287.73 1.00 1719.90 267.19 5.88 0.94
M2 2452.34 1.00 1883.00 290.15 6.18 0.95
M3 2365.02 1.00 1824.00 282.20 6.16 0.94
T T1 2227.37 1.00 1739.00 278.99 6.10 0.95
T2 2190.50 1.00 1611.00 261.28 5.83 0.95
T3 2253.29 1.00 1615.00 252.55 5.78 0.94
细菌Bacteria M M1 8258.27 0.99 6926.00 420.85 10.71 1.00
M2 8434.04 0.99 7110.00 429.44 10.73 1.00
M3 8314.83 0.99 7038.00 429.44 10.72 1.00
T T1 8352.32 0.99 6942.00 425.28 10.54 1.00
T2 8420.07 0.99 7008.00 423.80 10.68 1.00
T3 8330.19 0.99 7066.00 426.08 10.72 1.00

Fig.5

Relative abundance of different samples of bacteria at the phylum level"

Table 2

The proportion of bacteria in each sample at the phylum level under different film mulching treatments %"

处理
Treatment		 样品
Sample		 变形菌门
Proteobacteria		 酸杆菌门
Acidobacteriota		 放线菌门
Actinobacteriota		 芽单胞菌门
Gemmatimonadota		 拟杆菌门
Bacteroidota		 绿弯菌门
Chloroflexi		 粘菌门
Myxococcota		 浮霉菌门
Planctomycetota		 其他
Others
M M1 33.71 10.44 10.26 10.30 8.37 5.71 5.33 4.35 8.08
M2 33.38 12.09 8.25 10.50 6.89 6.16 5.62 5.23 8.38
M3 34.72 10.57 10.96 7.82 7.02 7.28 5.01 5.15 8.36
均值 33.94 11.03 9.82 9.54 7.43 6.38 5.32 4.91 8.27
T T1 37.23 10.13 5.92 9.13 11.59 4.91 5.12 4.10 7.38
T2 37.37 8.30 12.02 8.28 7.55 6.77 4.76 3.75 8.46
T3 33.93 10.43 11.71 7.49 6.25 8.92 4.07 5.36 8.82
均值 36.17 9.62 9.88 8.30 8.46 6.87 4.65 4.40 8.22

Fig.6

Relative abundance of different samples of fungus at the phylum level"

Table 3

The proportion of fungus in each sample at the fungi level under different film mulching treatments %"

处理
Treatment		 样品
Sample		 子囊菌门
Ascomycota		 未确定
Unidentified		 担子菌门
Basidiomycota		 被孢菌门
Mortierellomycota		 球囊菌门
Glomeromycota		 其他
Others
M M1 78.16 10.57 4.04 4.34 2.10 0.79
M2 65.22 10.14 18.47 3.24 1.90 1.04
M3 75.12 10.94 6.04 4.25 2.28 1.37
均值 72.83 10.55 9.51 3.94 2.09 1.07
T T1 65.82 13.63 13.44 4.01 1.71 1.40
T2 66.68 17.82 8.00 4.36 1.75 1.39
T3 69.21 16.79 6.84 3.54 2.05 1.58
均值 67.24 16.08 9.43 3.97 1.83 1.46

Fig.7

Relative abundance of different samples of bacteria at the genus level"

Table 4

The proportion of bacteria in each sample at the genus level under different film mulching treatments %"

处理
Treatment		 样品
Sample		 未确定
Unidentified		 未分类
Unclassified		 未培养菌
Uncultured-bacterium		 MND1 嗜盐粘细菌
Haliangium		 杆菌属
Metagenome		 RB41 溶杆菌属
Lysobacter		 鞘脂单胞菌属
Sphingomonas		 其他
Others
M M1 23.37 18.90 11.07 2.20 1.57 1.53 1.31 0.84 1.06 38.15
M2 25.68 18.13 10.62 2.28 1.82 1.69 1.79 0.78 1.09 36.14
M3 22.13 18.22 10.84 1.73 1.46 1.46 1.29 0.84 1.10 40.95
均值 23.72 18.42 10.84 2.07 1.61 1.56 1.46 0.82 1.08 38.41
T T1 21.99 19.00 9.52 2.15 1.72 1.55 1.74 1.77 0.98 39.58
T2 21.43 18.69 10.15 1.95 1.42 1.24 0.87 1.24 0.93 42.08
T3 21.62 18.59 11.01 1.79 1.32 1.25 1.18 1.02 0.94 41.30
均值 21.68 18.76 10.23 1.96 1.49 1.34 1.26 1.34 0.95 40.98

Fig.8

Relative abundance of different samples of fungi at the genus level"

Table 5

The proportion of fungi in each sample at the genus level under different film mulching treatments %"

处理
Treatment		 样品
Sample		 未确定
Unidentified		 外瓶霉属
Exophiala		 绿僵菌属
Metarhizium		 帚枝霉属
Sarocladium		 球孢毛葡孢霉属
Botryotrichum		 锥毛壳属
Coniochaeta		 瓶毛壳属
Lophotrichus		 镰刀菌属
Fusarium		 其他
Others
M M1 28.18 9.90 16.97 2.66 5.94 13.13 5.01 2.44 15.78
M2 36.62 10.83 15.48 2.92 6.18 3.38 2.73 2.97 18.88
M3 25.80 16.68 17.62 5.20 5.44 2.16 4.94 1.98 20.18
均值 30.20 12.47 16.69 3.59 5.85 6.22 4.23 2.47 18.28
T T1 52.73 6.40 1.49 15.03 1.36 0.32 1.56 1.67 19.43
T2 46.16 10.17 5.04 14.96 1.58 0.28 2.37 1.75 17.68
T3 44.55 11.49 3.68 15.80 1.92 0.26 2.40 1.44 18.45
均值 47.82 9.36 3.40 15.26 1.62 0.29 2.11 1.62 18.52
[1] Zhang Y C, Liu P, Wang C, et al. Genome-wide association study uncovers new genetic loci and candidate genes underlying seed chilling-germination in maize. PeerJ, 2021, 6(9):e11707
[2] 邓潜鑫. 施磷对紫色土榨菜―玉米根际微生物群落结构的影响. 重庆: 西南大学, 2022.
[3] 刘泉成. 玉米根际微生物群落特征分析及生防菌筛选. 北京: 中国农业科学院, 2018.
[4] 刘美霞, 刘秀, 赵燕, 等. 地膜覆盖对旱作春玉米农田土壤微生物碳源代谢影响研究. 生态学报, 2022, 42(22):1-13.
[5] 张旭东. 覆膜种植和施肥对半干旱地区资源高效利用及玉米生产持续性的影响机制. 杨凌: 西北农林科技大学, 2020.
[6] Li C J, Xiong Y W, Cui Z, et al. Effect of irrigation and fertilization regimes on grain yield, water and nitrogen productivity of mulching cultivated maize (Zea mays L.) in the Hetao Irrigation District of China. Agricultural Water Management, 2020, 232(1):106065.
[7] Lang Q, Li Y B, Xu Y Z, et al. Effect of film mulching and microbial inoculation on maize growth and water use efficiency under drought stress. The Journal of Applied Ecology, 2018, 29 (9):2915-2924.
[8] Sloan S S, Lebeis S L. Exercising influence: distinct biotic interactions shape root microbiomes. Current Opinion in Plant Biology, 2015, 26(8):32-36.
[9] Coats V C, Rumpho M E. The rhizosphere microbiota of plant invaders: an overview of recent advances in the microbiomics of invasive plants. Frontiers in Microbiology, 2014, 5(5):368.
[10] Shimurah H, Sadamoto M, Matsuura M, et al. Characterization of mycorrhizal fungi isolated from the threatened cypripedium macranthos, in a northern island of Japan: two phylogenetically distinct fungi associated with the orchid. Mycorrhiza, 2009, 19 (8):525-534.
doi: 10.1007/s00572-009-0251-4 pmid: 19449040
[11] Takakura Y. Tricholoma matsutake fruit bodies secrete hydrogen peroxide as a potent inhibitor of fungal growth. Canadian Journal of Microbiology, 2015, 61(6):447-450.
[12] Shaikh M N, Kasabe U I, Mokat D N. Influence of rhizosphere fungi on essential oil production and menthol content in Mentha arvensis L.. Journal of Essential Oil-Bearing Plants, 2018, 21(4):1076-1081.
[13] Dong W Y, Si P F, Liu E K, et al. Influence of film mulching on soil microbial community in a rainfed region of northeastern China. Scientific Reports, 2017, 7(1):8468.
doi: 10.1038/s41598-017-08575-w pmid: 28814759
[14] Wang J, Huang M K, Wang Q, et al. LDPE microplastics significantly alter the temporal turnover of soil microbial communities. The Science of the Total Environment, 2020, 726(7):138682.
[15] 李华伟, 罗文彬, 许国春, 等. 基于高通量测序的福建北部马铃薯晚疫病株根际土壤细菌群落分析. 微生物学通报, 2022, 49(3):1017-1029.
[16] 邱丽丽, 张佳宝, 赵炳梓. 土壤干旱对两品种小麦根际土壤微生物群落组成和酶活性的影响. 干旱区资源与环境, 2022, 36(2):116-122.
[17] Peiffer J A, Spor A, Koren O, et al. Diversity and heritability of the maize rhizosphere microbiome under field conditions. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(16):6548-6553.
[18] Edwards J, Johnson C, Santos-Medellín C, et al. Structure, variation, and assembly of the root-associated microbiomes of rice. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(8):911-920.
[19] Li X G, Jousset A, de Boer W, et al. Legacy of land use history determines reprogramming of plant physiology by soil microbiome. The ISME Journal, 2019, 13(3):738-751.
[20] 朱琳, 曾椿淋, 李雨青, 等. 基于高通量测序的大豆连作土壤细菌群落多样性分析. 大豆科学, 2017, 36(3):419-424.
[21] Cheng L, Booker F L, Tu C, et al. Arbuscular mycorrhizal fungi increase organic carbon decomposition under elevated CO2. Science, 2012, 337(6098):1084-1087.
doi: 10.1126/science.1224304 pmid: 22936776
[22] Traoré O Y A, Kiba D I, Arnold M C, et al. Fertilization practices alter microbial nutrient limitations after alleviation of carbon limitation in a Ferric Acrisol. Biology and Fertility of Soils, 2016, 52(2):177-189.
[23] 宋秋华. 半干旱黄土高原区地膜覆盖春小麦土壤微生物特征与养分转化. 兰州: 兰州大学, 2006.
[24] 段翠花. 不同类型地膜对宁南山区农田土壤环境的影响研究. 杨凌: 西北农林科技大学, 2022.
[25] 丁柳屹, 王森, 付鑫. 覆盖条件下旱作春玉米农田土壤细菌群落结构分析. 地球环境学报, 2019, 10(6):590-600.
[26] Huang F Y, Liu Z H, Mou H Y, et al. Effects of different long-term farmland mulching patterns on the loessial soil fungal community in a semiarid region of China. Applied Soil Ecology, 2019, 1:111-119.
[27] 胡志娥, 肖谋良, 丁济娜, 等. 长期覆膜条件下农田土壤微生物群落的响应特征. 环境科学, 2022, 43(10):4745-4754.
[28] 徐雪雪. 基于高通量测序的马铃薯沟垄覆膜连作土壤微生物多样性分析. 兰州: 甘肃农业大学, 2016.
[29] Yu P, Wang C, Baldauf J A, et al. Root type and soil phosphate determine the taxonomic landscape of colonizing fungi and the transcriptome of field-grown maize roots. The New Phytologist, 2018, 217 (3):1240-1253.
[30] Voges M J E E E, Bai Y, Schulze-Lefert P, et al. Plant-derived coumarins shape the composition of an Arabidopsis synthetic root microbiome. Proceeding of the National Academy of Sciences of the United States of America, 2019, 116(25):12558- 12565.
[31] Cotton T E A, Pétriacq P, Cameron D D, et al. Metabolic regulation of the maize rhizobiome by benzoxazinoids. The ISME Journal, 2019, 13(7):1647-1658.
[32] Yu P, He X, Baer M, et al. Plant flavones enrich rhizosphere Oxalobacteraceae to improve maize performance under nitrogen deprivation. Nature Plants, 2021, 7(4):481-499.
doi: 10.1038/s41477-021-00897-y pmid: 33833418
[33] 李越鲲, 孙燕飞, 雷勇辉, 等. 枸杞根际土壤真菌群落多样性的高通量测序. 微生物学报, 2017, 57(7):1049-1059.
[34] 付亚娟, 张江丽, 侯晓强. 大花杓兰根际与非根际土壤真菌多样性的高通量测序分析. 西北农业学报, 2019, 28(2):253-259.
[35] 黄方园. 覆盖模式对不同旱作区农田土壤主要性状和玉米生长的影响. 杨凌: 西北农林科技大学, 2020.
[36] Fan W, Wu J G. Short-term effects of returning granulated straw on soil microbial community and organic carbon fractions in dryland farming. The Journal of Microbiology, 2020, 58(8): 657-667.
[37] Leff J W, Jones S E, Prober S M, et al. Consistent responses of soil microbial communities to elevated nutrient inputs in grasslands across the globe. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(35):10967- 10972.
[1] Yang Yanming, Zhou Yi, Zhang Zijian, Zhao Yang, Li Yajian, Pang Yaqi, Wang Yaxun, Han Wenyu, Liu Jinghui. Effects of Humic Acid and Different Tillage Measures on Carbon Pool and Microbial Community Structure in Saline Alkali Soil [J]. Crops, 2024, 40(1): 157-165.
[2] Zhang Rong, Chen Xiaowen, Lu Ping, You Yanrong, Zhou Delu, Li Deming. Effects of Different Mulching Modes on Soil Moisture, Temperature and Yield of Potato in Dry Land [J]. Crops, 2023, 39(5): 145-150.
[3] Tian Xiaoqin, Wang Dan, Li Zhuo, Liu Yonghong, Li Wei. Effects of Ridge and Mulching on Yield and Water Use Efficiency of Rapeseed [J]. Crops, 2023, 39(5): 204-211.
[4] Song Xiao, Zhang Keke, Yue Ke, Huang Chenchen, Huang Shaomin, Sun Jianguo, Guo Tengfei, Guo Doudou, Zhang Shuiqing, Pei Minnan. Differences of Enzyme Activities and Bacterial Communities in Rhizosphere Soil of Wheat Varieties with Different Nitrogen Efficiency [J]. Crops, 2023, 39(4): 188-194.
[5] Liu Sujun, Meng Meilian, Suriguga . Research on the Effects of Gene Expression in Sugar Metabolism Pathway of Potato by Drought Stress and Rehydration [J]. Crops, 2023, 39(1): 38-45.
[6] Wen Rui, Chen Qianwu, Zhao Yajie, Jia Yiming, Lu Xudong, Zhang Jihong, Li Huanchun, Zhao Peiyi, Zhang Yonghu. Study on Water Temperature Effects and Water Use Efficiency of Paddy Field under Different Plastic Film Mulching Planting Patterns in Arid Area of Loess Plateau in Northwest China [J]. Crops, 2022, 38(6): 111-117.
[7] Sun Zixin, Cai Baiyan. Research Advances on the Effects of Continuous Cropping on Soil Microbial Community and Restoration Techniques [J]. Crops, 2022, 38(6): 7-13.
[8] Li Yanlu, Wang Junpeng, Yu Xinzhi, Wei Honglei, Chen Qiyu, Zhao Hongxiang, Xu Chen, Bian Shaofeng, Zhang Zhian. Effects of Mulching Different Plastic Films on Accumulation and Distribution of Dry Matter and Nitrogen in Maize in Cold and Cool Areas [J]. Crops, 2022, 38(5): 124-129.
[9] Xu Yan, Niu Junfeng, Chen Lijun, Wang Shiqiang, Dong Zhongmin, Wang Zhezhi. Study of Rhizosphere Soil Microbial Changes of Atractylodes lancea Based on High-Throughput Sequencing [J]. Crops, 2022, 38(5): 221-228.
[10] Yang Zhinan, Huang Jinwen, Han Fanxiang, Li Yawei, Ma Jiantao, Chai Shouxi, Cheng Hongbo, Yang Delong, Chang Lei. Effects of Straw Strip Mulching on Soil Temperature and Yield of Potato Field in Rain-Fed Region in Northwest China [J]. Crops, 2022, 38(1): 196-204.
[11] Lin Ruxia, Guo Fengdan, Wang Xingjun, Xia Han, Hou Lei. Advances in Peanut Molecular Breeding [J]. Crops, 2021, 37(5): 1-5.
[12] Hu Qiguo, Liu Yajun, Wang Wenjing, Wang Qi, Wang Honggang, Chu Fengli. Effects of Sweet Potato Rotation and Intercropping on the Microbial Community of Rhizosphere Soil [J]. Crops, 2021, 37(5): 153-159.
[13] Liu Akang, Wang Demei, Wang Yanjie, Yang Yushuang, Ma Ruiqi, Gao Tiantian, Wang Yujiao, Kan Mingxi, Zhao Guangcai, Chang Xuhong. Effects of Seedling Regulation on Yield and Nitrogen Utilization of Late Sowing Wheat [J]. Crops, 2021, 37(2): 116-123.
[14] Liang Xiaohong,Zhang Ruidong,Huang Minjia,Liu Jing,Cao Xiong. Interaction of Film Mulching and Nitrogen Application on Yield, Water and Nitrogen Use Efficiency of Sorghum [J]. Crops, 2019, 35(5): 135-142.
[15] Yan Wei,Li Guolong,Li Zhi,Cao Yang,Zhang Shaoying. Effects of Nitrogen Application Rate and Planting Density Interaction on Photosynthetic Characteristics and Root Yield of Sugar Beet under Full-Film Mulching in Arid Regions [J]. Crops, 2019, 35(4): 100-106.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!