Crops ›› 2025, Vol. 41 ›› Issue (5): 54-60.doi: 10.16035/j.issn.1001-7283.2025.05.008

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Response of Mung Bean Genotypes to Exogenous Brassinosteroids under Saline-Alkali Stress

Zhang Jindong(), Wang Cheng(), Lu Huan, Zeng Lingling, Zhang Gongliang, Sun Haoyue, Liu Yue, Yang Helin, Hou Xiaomin   

  1. Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161000, Heilongjiang, China
  • Received:2024-08-21 Revised:2024-11-05 Online:2025-10-15 Published:2025-10-21

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Abstract:

This study investigated the regulatory effects of exogenous brassinosteroids (BR) on mung bean seedlings under saline-alkali stress. Three genotypes (?Lüfeng 2?, ?Nenlü 4?, and ?Zhonglü 27?) were used, with BR applied at concentrations of 0.5, 1.0, 1.5, and 2.0 mg/L. The results showed that saline-alkali stress inhibited root growth, reduced nutrient uptake, decreased photosynthetic pigment content, and lowered photosynthetic capacity, leading to reduced dry matter accumulation. The content of photosynthetic pigments in seedlings stage decreased, and their photosynthetic capacity weakened, resulting in a reduction in dry matter accumulation. Spraying BR treatment could significantly increase the chlorophyll content of mung bean seedlings stage. Under the treatment of 1.5 m/L, the total root length and root surface area of the underground part significantly increased. Meanwhile, by regulating the enhancement of the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in the plant body, and the increase in the contents of osmotic adjustment substances such as soluble protein, proline, and soluble sugar, the damage caused by the saline-alkali environment was significantly reduced. This treatment also enhanced the activity of antioxidant enzymes and increased the content of osmotic adjustment substances, thereby greatly reducing the damage caused by the saline-alkali environment.

Key words: Mung bean, Saline-alkali stress, Brassinosteroids, Seedling growth, Chlorophyll content, Antioxidant enzyme activity

Table 1

Effects of different concentrations of BR on the growth and development of aboveground parts of mung beans under saline-alkali stress during seedling stage"

品种
Variety		 处理
Treatment		 株高
Plant height (cm)		 干物质积累量
Dry matter accumulation (g)
绿丰2号
Lüfeng 2		 CK 17.09±0.53a 0.30±0.01a
T1 9.46±0.30d 0.17±0.01e
T2 12.96±0.22c 0.19±0.01de
T3 12.76±0.26c 0.24±0.01b
T4 14.86±0.76b 0.21±0.01cd
T5 13.61±0.18bc 0.23±0.01bc
嫩绿4号
Nenlü 4		 CK 17.79±0.61a 0.37±0.01a
T1 10.48±0.33d 0.19±0.01c
T2 13.00±0.17c 0.21±0.02bc
T3 13.77±0.44bc 0.23±0.01b
T4 15.11±0.40b 0.21±0.01bc
T5 12.53±0.49c 0.23±0.01b
中绿27
Zhonglü 27		 CK 17.79±0.61a 0.49±0.01a
T1 18.17±0.46a 0.20±0.01d
T2 11.21±0.29d 0.21±0.01cd
T3 13.32±0.30c 0.29±0.01b
T4 13.80±0.39c 0.26±0.01b
T5 15.40±0.38b 0.25±0.02bc
F值
F-value		 品种 4.95* 93.78**
浓度 91.52** 264.00**
品种×浓度 2.25* 19.35**

Table 2

Effects of different concentrations of BR on root growth and development under saline-alkali stress during mung bean seedling stage"

品种
Variety		 处理
Treatment		 根系总长
Total root length (mm)		 根系干重
Root dry weight (g)		 根体积
Root volume (cm3)		 根表面积
Root surface area (cm2)
绿丰2号Lüfeng 2 CK 447.12±7.59a 0.06±0.01a 0.04±0.01a 39.14±2.58a
T1 93.63±3.35e 0.01±0.01c 0.01±0.01d 12.19±1.97d
T2 105.37±6.13de 0.02±0.01bc 0.02±0.01c 15.25±0.90cd
T3 133.36±6.40cd 0.02±0.01b 0.02±0.01b 16.32±1.65cd
T4 213.92±14.10b 0.02±0.01bc 0.02±0.01c 21.63±0.25b
T5 151.20±8.72c 0.02±0.01b 0.02±0.01b 18.55±0.56bc
嫩绿4号Nenlü 4 CK 112.75±16.58a 0.06±0.01a 0.04±0.01a 37.61±2.22a
T1 119.10±6.97d 0.02±0.01c 0.01±0.01c 15.10±1.65d
T2 143.17±7.56d 0.02±0.01c 0.02±0.01c 17.11±0.86cd
T3 147.76±4.39c 0.02±0.01c 0.02±0.01b 20.02±0.95bc
T4 201.82±7.62b 0.02±0.01c 0.02±0.01c 22.99±1.25b
T5 127.76±2.58cd 0.03±0.01b 0.02±0.01b 19.41±0.02bcd
中绿27 Zhonglü 27 CK 391.14±5.18a 0.10±0.01a 0.06±0.01a 59.62±7.14a
T1 104.75±1.68d 0.02±0.01c 0.02±0.01b 14.75±0.66c
T2 112.63±4.55d 0.04±0.01b 0.02±0.01b 19.89±0.77bc
T3 187.42±3.21b 0.04±0.01b 0.03±0.01b 21.97±0.67bc
T4 188.40±9.74b 0.03±0.01bc 0.02±0.01b 27.15±1.29b
T5 151.46±9.23c 0.03±0.01bc 0.04±0.02ab 21.68±0.16bc
F值F-value 品种 8.85** 173.86** 8.68** 41.34***
浓度 1220.19** 392.65** 26.80** 195.11**
品种×浓度 14.30** 20.05** 1.26 9.86**

Fig.1

The effect of different concentrations of BR on chlorophyll a content in mung bean under saline-alkali stress during seedling stage Different letters indicate significant difference in treatments of the same variety (P < 0.05), the same below."

Fig.2

The effect of different concentrations of BR on chlorophyll b in mung bean under saline-alkali stress during seedling stage"

Fig.3

The effect of different concentration of BR on the total chlorophyll content in mung bean under saline-alkali stress during seedling stage"

Table 3

Effects of different concentrations of BR on osmotic regulators under saline-alkali stress in mung bean during seedling stage"

品种
Variety		 处理
Treatment		 可溶性糖
Soluble sugar
(mg/g)		 可溶性蛋白
Soluble protein
(mg/g)		 脯氨酸
Proline
(μg/g)
绿丰2号
Lüfeng 2		 CK 3.62±0.08c 17.11±0.98ab 11.03±0.32d
T1 3.96±0.10bc 11.00±0.39d 11.28±0.24d
T2 4.66±0.26b 16.19±0.96ab 12.00±0.39cd
T3 4.27±0.38bc 14.62±0.10bc 17.19±0.43b
T4 6.10±0.48a 12.86±0.54cd 12.97±0.22c
T5 4.58±0.18bc 19.04±1.61a 18.91±0.48a
嫩绿4号
Nenlü 4		 CK 4.12±0.14c 18.36±0.12a 11.95±0.07d
T1 4.36±0.15c 11.88±0.77c 11.92±0.14d
T2 5.18±0.11b 15.02±0.98b 13.45±0.23c
T3 5.13±0.14b 12.36±0.35c 16.44±0.13b
T4 5.91±0.24a 12.44±0.25c 12.61±0.09cd
T5 4.69±0.25bc 17.12±0.93ab 20.77±0.62a
中绿27
Zhonglü 27		 CK 4.88±0.10c 16.36±1.02ab 10.84±0.19d
T1 5.05±0.05c 13.03±0.84bc 11.80±0.10d
T2 5.35±0.06b 15.74±1.23ab 15.03±0.35c
T3 5.45±0.10b 15.06±1.58ab 21.81±0.21a
T4 6.80±0.05a 11.51±0.20c 14.72±0.28c
T5 4.40±0.04d 16.87±0.82a 20.61±0.44b
F值
F-value		 品种 46.76** 1.61 112.08**
浓度 80.99** 51.54** 800.63**
品种×浓度 5.08** 3.32** 31.31**

Fig.4

The effect of different concentrations of BR on SOD activity under saline-alkali stress during mung bean seedling stage"

Fig.5

The effect of different concentrations of BR on POD activity under saline-alkali stress during mung bean seedling stage"

Fig.6

The effect of different concentrations of BR on CAT activity under saline-alkali stress during mung bean seedling stage"

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