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作物杂志,2025, 第5期: 135–141 doi: 10.16035/j.issn.1001-7283.2025.05.018

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

水稻CYP450家族基因Os78A5的表达分析

植藓闳1(), 纪子贤1, 许镇旺1, 谭恩1, 梁日深1, 马帅鹏2, 唐辉武1()   

  1. 1 仲恺农业工程学院农业与生物学院， 510225， 广东广州
    2 中国热带农业科学院广州实验站， 510145， 广东广州
  • 收稿日期:2024-06-14 修回日期:2024-07-24 出版日期:2025-10-15 发布日期:2025-10-21
  • 通讯作者: 唐辉武，研究方向为水稻重要农艺性状分子机理，E-mail：huiwutang@zhku.edu.cn
  • 作者简介:植藓闳，研究方向为农艺与种业，E-mail：1147803854@qq.com
  • 基金资助:
    广东省自然科学基金(2022A1515010798);清远市科技计划项目(2023KJJ005);仲恺农业工程学院仲华基因产业学院项目(ZHCY2023007);海南省自然科学基金面上项目(320MS079)

Expression Analysis of the CYP450 Family Gene Os78A5 in Rice

Zhi Xianhong1(), Ji Zixian1, Xu Zhenwang1, Tan En1, Liang Rishen1, Ma Shuaipeng2, Tang Huiwu1()   

  1. 1 College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong, China
    2 Guangzhou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Guangzhou 510145, Guangdong, China
  • Received:2024-06-14 Revised:2024-07-24 Online:2025-10-15 Published:2025-10-21

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摘要： 细胞色素P450（cytochrome P450，CYP450）广泛存在于各种生物中，在植物次生代谢和胁迫响应过程中具有不可或缺的作用。在水稻（Oryza sativa）中克隆了一个编码CYP450蛋白的基因Os78A5（LOC_Os11g29720），并利用生物信息学技术分析Os78A5基因结构、演化过程和顺式作用元件，同时利用实时荧光定量PCR（quantitative real-time PCR，qRT-PCR）技术分析Os78A5的组织表达特异性及其对不同激素和非生物胁迫的响应情况。结果表明，Os78A5编码区长度1617 bp，编码538个氨基酸。Os78A5与狗尾草（Setaria viridis）和高粱（Sorghum bicolor）的同源蛋白CYP78A5具有较近的亲缘关系。Os78A5的启动子区包含48个激素响应元件、23个环境胁迫响应元件和35个水分响应顺式作用元件。Os78A5在水稻叶片中的表达水平显著高于其他组织，且受细胞分裂素、赤霉素、生长素、脱落酸、高温和低温等诱导表达，推测Os78A5可能参与水稻的非生物胁迫响应。

关键词: 水稻, CYP450, 非生物胁迫, 植物激素, 表达分析

Abstract:

Cytochrome P450 (CYP450) is widely present in various organisms and plays an essential role in plant secondary metabolism and stress response. A gene encoding the CYP450 protein, Os78A5 (LOC_ Os11g29720), was cloned from rice (Oryza sativa). The gene structure, evolution process and cis-regulatory elements of Os78A5 were analyzed by bioinformatics method, and the expression of Os78A5 in different tissues and response to different plant hormones and abiotic stresses were analyzed by quantitative real-time PCR (qRT-PCR). The results showed that coding region of Os78A5 was 1617 bp, encoding 538 amino acids. Os78A5 has closer evolutionary relationship with the homologous protein CYP78A5 of Setaria viridis and Sorghum bicolor. The promoter region of Os78A5 contained 48 hormone response elements, 23 environmental stress response elements and 35 water response cis-regulatory elements. The expression of Os78A5 in rice leaves was significantly higher than that in other tissues, and it was induced by 6-benzylaminopurine, gibberellic acid, indole acetic acid, abscisic acid, high temperature and low temperature, suggesting that Os78A5 might be involved in abiotic stress response in rice.

Key words: Rice, CYP450, Abiotic stress, Plant hormone, Expression analysis

表1

qRT-PCR所用引物序列

引物名称Primer name 引物序列（5′-3′）Primer sequence (5′-3′)
Os78A5-F TGCTGTCCTCTGGGAGATGAT
Os78A5-R GAAGGTTGGGGATGTCCGAG
Actin1-F GCATCTCTCAGCACATTCCA
Actin1 -R ACCACAGGTAGCAATAGGTA

图1

Os78A5基因结构和蛋白结构域 灰色框为3′ UTR区和5′ UTR区，黑色方框为外显子，黑色细线为内含子，白色框为TMH结构域。

图2

Os78A5的进化分析 百分比数值为进化分支的可靠程度。

表2

Os78A5启动子顺式作用元件分析

元件名称
Name of element		 功能
Function		 数量Number
ABRELATERD1 脱水响应 2
ACGTATERD1 脱水响应 4
CBFHV 脱水响应 4
DRECRTCOREAT 脱水响应 2
MYB1AT 脱水响应 4
MYB2CONSENSUSAT 脱水响应 2
MYCCONSENSUSAT 脱水响应和冷胁迫响应 12
MYBCORE 水分响应 3
MYB2AT 水分响应 2
SEBFCONSSTPR10A IAA响应 2
SURECOREATSULTR11 IAA响应 6
ARFAT IAA响应 2
ASF1MOTIFCAMV IAA、水杨酸响应 5
WBOXATNPR1 水杨酸诱导响应 2
GAREAT GA响应元件 1
MYBGAHV GA响应 1
PYRIMIDINEBOXOSRAMY1A GA响应 1
WRKY71OS GA响应 22
CPBCSPOR 6-BA响应 1
LTRECOREATCOR15 低温响应、ABA响应 3
CCAATBOX1 高温胁迫响应 6
GT1GMSCAM4 盐胁迫响应 2

图3

Os78A5的基因表达模式分析

图4

不同激素处理下Os78A5表达水平分析 “*”：P < 0.05，“**”：P < 0.01，“***”：P < 0.001。下同。

图5

不同胁迫处理下Os78A5表达水平分析

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