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作物杂志,2026, 第5期: 42–50 doi: 10.16035/j.issn.1001-7283.2026.05.005

• 植物激素与作物生长发育调控专题 • 上一篇    下一篇

水稻赤霉素受体GID1L2的表达分析

纪子贤(), 李乐晨, 蔡欣, 陈才敏, 丁佳淳, 黄志濠, 李克克()   

  1. 仲恺农业工程学院农业与生物学院， 510225， 广东广州
  • 收稿日期:2025-07-17 修回日期:2025-08-16 出版日期:2026-10-15 发布日期:2026-10-22
  • 通讯作者: 李克克，研究方向为水稻重要农艺性状的分子机理以及生长素响应因子功能，E-mail：kekeli1013@163.com
  • 作者简介:纪子贤，研究方向为生物技术，E-mail：zixianji@foxmail.com
  • 基金资助:
    广州市基础研究计划基础与应用研究专题(2023A04J1430);大学生创新创业训练计划项目(202411347009)

Expression Analysis of Rice Gibberellin Receptor GID1L2

Ji Zixian(), Li Yuechen, Cai Xin, Chen Caimin, Ding Jiachun, Huang Zhihao, Li Keke()   

  1. College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong, China
  • Received:2025-07-17 Revised:2025-08-16 Online:2026-10-15 Published:2026-10-22

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摘要：

赤霉素作为重要的植物激素成员之一，通过与赤霉素受体结合，参与植物生长发育的调控过程。本研究克隆了水稻赤霉素受体基因Gibberellin Insensitive Dwarf1 Like2（OsGID1L2），分析其蛋白结构功能及表达特性。结果表明，OsGID1L2编码1个由320个氨基酸和Abhydrolase_3保守结构域组成的蛋白。同源序列比对和遗传进化树分析发现，OsGID1L2与单子叶植物具有较近的亲缘关系。OsGID1L2启动子区域含有大量的环境因子和激素响应元件，试验进一步证明OsGID1L2受到吲哚-3-乙酸、赤霉素、6-苄基腺嘌呤和脱落酸等激素和温度的诱导表达。OsGID1L2在根、茎、叶和穗中均有表达，在叶片中的表达量最高，其表达蛋白定位于细胞质和细胞核中。这些结果揭示了OsGID1L2的表达模式，以及在激素和温度响应调控中的重要作用，为进一步深度剖析赤霉素受体家族的功能机制奠定了基础。

关键词: 水稻, 赤霉素受体, OsGID1L2, 亚细胞定位, 非生物胁迫, 表达分析

Abstract:

Gibberellins, as one of the important plant hormones, are involved in the regulatory process of plant growth and development by binding to gibberellin receptors. We cloned the rice gibberellin receptor gene Gibberellin Insensitive Dwarf1 Like2 (OsGID1L2), and analyzed its protein structure and function as well as expression characteristics. The results showed that OsGID1L2 encodes a protein of 320 amino acids containing the conserved domain of Abhydrolase_3. Homology sequence alignment and phylogenetic tree analysis revealed that OsGID1L2 was closely related to monocotyledonous plants. The promoter region of OsGID1L2 contained numerous environmental factor and hormone response elements. Experimental results further confirmed that OsGID1L2 was induced by hormones such as indole-3-acetic acid, gibberellins, 6-benzyladenine and abscisic acid, and by temperature. OsGID1L2 was expressed in roots, stems, leaves, and panicles, with the highest expression level in leaves. The expressed protein was localized in the cytoplasm and nucleus. These results reveal that OsGID1L2 has an important role in the regulation of hormone and temperature responses, and lay the foundation for further in-depth analysis of the functional mechanism of the gibberellin receptor family.

Key words: Rice, Gibberellin receptor, OsGID1L2, Subcellular localization, Abiotic stress, Expression analysis

表1

本研究所用引物

用途Usage 引物Primer 引物序列（5′-3′）Primer sequence (5′-3′)
qRT-PCR GID1L2-qF CATTCAGCTGGCAGAAGAGAAT
GID1L2-qR TCACCCTAGTTTCAGGGATAT
Actin1-qF GCATCTCTCAGCACATTCCA
Actin1-qR ACCACAGGTAGCAATAGGT
CDS扩增
CDS amplification		 Primer-F ACTACTGAATTCATGGATTCCAGCAGCGCCGAGATCCT
Primer-R AGCTATGGTACCAATGGCCAGCAATGAAAGCAGCCACT

图1

OsGID1L2蛋白的结构预测与分析 (a) OsGID1L2的结构域，数字为对应的氨基酸位置，橙色区域为Abhydrolase结构域。(b) 蛋白结构模型重合一致性图；(c) 结构集合方差评估图；(d~e) OsGID1和OsGID1L2蛋白的结构重合图，分别为正视图与后视图，蓝色的为GID1L2蛋白预测结构，黄色的为GID1蛋白结构。

图2

OsGID1L2及其同源蛋白的氨基酸序列比对

图3

OsGID1L2及其同源蛋白的进化关系分析

表2

OsGID1L2启动子上与逆境响应相关的主要顺式作用元件

顺式作用元件类型
Types of cis-acting element		 顺式作用元件
Cis-acting element		 数量
Number
激素响应
Hormone response		 细胞分裂素调控因子结合元件 2
ABA响应元件 6
GA响应元件 1
水杨酸响应元件 1
IAA和类IAA响应元件 2
茉莉酸响应 4
环境响应
Environment response		 光响应 13
干旱响应元件 3
低温响应元件 2
高盐和寒冷胁迫响应元件 2
多种非生物胁迫响应元件 10
转录调控
Transcript regulation		 MYB转录因子结合 4
增强子 22
类MYB转录因子结合元件 2
MYC转录因子结合元件 1

图4

OsGID1L2在水稻不同组织中的表达水平分析

图5

不同激素处理后OsGID1L2在水稻叶片中的表达水平分析 “**”代表差异达极显著（P < 0.01）水平：“***”代表差异达极显著（P < 0.001）水平。下同。

图6

不同胁迫处理后OsGID1L2在水稻叶片中的表达水平分析 “*”代表差异达显著（P < 0.05）水平。

图7

OsGID1L2扩增产物的琼脂凝胶电泳检测 1#、2#、3#均为OsGID1L2扩增产物。

图8

OsGID1L2蛋白的亚细胞定位 (a) GFP空载体在水稻原生质体瞬时表达；(b) OsGID1L2-GFP融合蛋白在水稻原生质体中的瞬时表达；(a)和(b)比例尺为10 µm。

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