大豆对胞囊线虫4号生理小种的抗性遗传分析

doi:10.16035/j.issn.1001-7283.2016.03.005

摘要/Abstract

摘要：

大豆胞囊线虫病（Soybean cyst nematode）严重危害我国大豆生产。我国大豆胞囊线虫有8个生理小种,其中,4号生理小种致病力最强,主要分布在黄淮海大豆产区。了解抗源的遗传模式有助于抗病基因的定位和分子标记的开发。以对大豆胞囊线虫4号生理小种高抗抗源CBL黑豆为父本、高感材料品75-14为母本,构建了F₁、F₂和F_2∶3 3个世代群体,利用植物数量性状主基因+多基因混合遗传模型的联合分离分析方法,分析CBL黑豆抗大豆胞囊线虫4号生理小种的遗传效应。结果表明：CBL黑豆对胞囊线虫4号生理小种的抗性受2对加性-显性-上位性主基因和加性-显性-上位性多基因控制,F₂世代主基因遗传率为64.47%,F_2∶3世代主基因遗传率为75.99%。主基因遗传率较高,育种可以在早代选择。

关键词: 大豆, 大豆胞囊线虫, 主基因+多基因, 遗传分析

Abstract:

Soybean cyst nematode (SCN) is a destructive pest in soybean production worldwide and causes great yield loss every year. There are 14 SCN races worldwide, among which 8 races are in china. Race 4 of SCN (SCN4), which is the predominant race in Huang-Huai-hai soybean production area, has the most virulence. A soybean cultivar-CBL, which was isolated from Chibuliuheidou with high resistance to SCN, but its growth period was shorter than Chibuliuheidou. In this paper, genetic model of CBL was studied, which will help for cloning of resistant gene and resistant marker exploitation. Three generations (F₁, F₂, F_2:3 ) were generated from the crosses between CBL and pin 75-14, which was a high susceptible to SCN4. Genetic model was analyzed by the method of mixed major gene plus polygene genetic model. The resistant inheritance to SCN4 was controlled by two additive-dominance-espistatic major genes and additive-dominance-espistatic polygenes. The F₂ and F_2:3 heritabilities of major genes were estimated to be 64.47% and 75.99%, respectively. So resistance plants can be selected in early filial generation.

Key words: Soybean, Soybean cyst nematode, Major gene plus polygene, Inheritance

张海平,吴书峰,王志. 大豆对胞囊线虫4号生理小种的抗性遗传分析[J]. 作物杂志, 2016 (3): 21–26

Haiping Zhang,Shufeng Wu,Zhi Wang. Inheritance of Resistance to Race 4 of Cyst Nematode in Soybean[J]. Crops, 2016(3): 21–26

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世代 Generation	病情指数分布Disease level distribution(株)											总数 (株) Total	平均病情指数(%) Disease index	标准差 SD
世代 Generation	0~ 10%	10.1%~ 20%	20.1%~ 30%	30.1%~ 40%	40.1%~ 50%	50.1%~ 60%	60.1%~ 70%	70.1%~ 80%	80.1%~ 90%	90.1%~ 100%	>100%	总数 (株) Total	平均病情指数(%) Disease index	标准差 SD
P₁											10	10	192.85	8.14
P₂	10											10	0.28	0.13
F₁										1	9	10	117.66	16.76
F₂	10	6	4	9	10	5	6	6	6	4	52	118	102.00	55.77
F_2∶3	8	6	3	4	6	17	13	13	6	11	31	118	72.03	27.25

模型代码 Model code	模型 Model	极大似然值 Max likelihood value	AIC值 AIC value
A1	1MG-AD	-4 039.66	8 087.326
A2	1MG-A	-4 041.22	8 088.441
A3	1MG-EAD	-4 040.94	8 087.885
A4	1MG-AEND	-4 040.94	8 087.881
B1	2MG-ADI	-3 785.66	7 591.325
B2	2MG-AD	-3 890.82	7 793.635
B3	2MG-A	-3 842.68	7 693.352
B4	2MG-EA	-4 281.48	8 568.954
B5	2MG-AED	-3 918.90	7 845.809
B6	2MG-EEAD	-4 525.45	9 056.901
C0	PG-ADI	-3 855.96	7 731.914
C1	PG-AD	-3 924.15	7 862.299
D0	MX1-AD-ADI	-3 814.41	7 648.811
D1	MX1-AD-AD	-3 911.74	7 839.481
D2	MX1-A-AD	-3 924.04	7 862.086
D3	MX1-EAD-AD	-3 922.40	7 858.794
D4	MX1-AEND-AD	-3 925.51	7 865.020
E0	MX2-ADI-ADI	-3 777.41	7 586.823
E1	MX2-ADI-AD	-3 779.71	7 587.411
E2	MX2-AD-AD	-3 911.74	7 843.481
E3	MX2-A-AD	-3 896.45	7 808.902
E4	MX2-EAED-AD	-3 924.00	7 861.996
E5	MX2-AED-AD	-3 880.57	7 777.141

模型Model	世代Generation	U₁²	U₂²	U₃²	nW²	Dn
B1	P₁	5.3455(0.0208)	21.3231(0.0000)	90.5607(0.0000)	8.4035(0.0121)	0.0096(1.0000)
	P₂	5.5307(0.0187)	21.6204(0.0000)	90.0765(0.0000)	8.4195(0.0122)	0.0096(1.0000)
	F₁	6.4420(0.0111)	24.9129(0.0000)	102.7199(0.0000)	9.4931(0.0166)	0.0086(1.0000)
	F₂	0.3390(0.5604)	1.3029(0.2537)	5.3397(0.0208)	3.1920(0.0002)	0.0042(1.0000)
	F_2∶3	1.1842(0.2765)	0.0000(0.9974)	17.8719(0.0000)	3.2920(0.0002)	0.0584(0.3824)
E0	P₁	3.5646(0.0590)	18.2294(0.0000)	95.3780(0.1035)	8.2496(0.0115)	0.0096(1.0000)
	P₂	3.5646(0.0590)	18.2294(0.0874)	95.3780(0.5347)	8.2496(0.1215)	0.0096(1.0000)
	F₁	2.9674(0.0850)	18.6210(0.0000)	112.1310(0.0000)	9.1941(0.0153)	0.0086(1.0000)
	F₂	1.9975(0.1576)	2.2509(0.1335)	0.2782(0.5979)	3.5021(0.3253)	0.0042(1.0000)
	F_2∶3	0.0740(0.7855)	0.6536(0.4188)	18.3838(0.0000)	3.1149(0.0902)	0.0397(0.8372)
E1	P₁	4.1602(0.0414)	19.3183(0.0000)	93.7310(0.0000)	8.3011(0.0117)	0.0096(1.0000)
	P₂	4.1602(0.0414)	19.3183(0.0000)	93.7310(0.0000)	8.3011(0.0117)	0.0096(1.0000)
	F₁	7.6119(0.0058)	26.7112(0.0000)	99.7542(0.0000)	9.5936(0.0170)	0.0086(1.0000)
	F₂	0.0866(0.7685)	0.3651(0.5457)	12.6517(0.0004)	3.1534(0.0002)	0.0042(1.0000)
	F_2∶3	1.2685(0.2600)	3.2737(0.0704)	8.2673(0.0040)	3.2366(0.0002)	0.0603(0.3435)

一阶遗传参数 1st order genentic parameter	估计值 Estimate	二阶遗传参数 2st order genentic parameter	估计值Estimate
一阶遗传参数 1st order genentic parameter	估计值 Estimate	二阶遗传参数 2st order genentic parameter	F₂	F_2∶3
m1(m)	-50.12	σ_f²	2 255.52	518.02
m2	9.79	σ_mg²	4 092.64	1 640.26
m3	18.12	σ_pg²	1 737.52	0
m4	78.99	σ_p²	6 348.18	2 158.28
m5	22.92	σ²	518.02	518.02
da(d)	18.11	h_mg²	64.47%	75.99%
db	18.43	h_pg²	27.37%	0
ha(h)	-14.51
hb	54.48
i	18.09
jab	55.18
jba	-15.66
l	-58.73