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作物杂志,2016, 第5期: 81–86 doi: 10.16035/j.issn.1001-7283.2016.05.014

• 生理生化·植物营养·栽培耕作 • 上一篇    下一篇

高温条件下CO2对黄瓜叶片光合速率和气孔特性的影响

张之为,李晓静,白金瑞,陈帅   

  1. 内蒙古农业大学农学院,010019,内蒙古呼和浩特
  • 收稿日期:2016-07-20 修回日期:2016-08-14 出版日期:2016-10-15 发布日期:2018-08-26
  • 作者简介:张之为,副教授,主要从事植物生理研究
  • 基金资助:
    内蒙古教育厅项目(NJZY14083);内蒙古自然科学基金(2015BS0313)

Effects of CO2 on Cucumber Leaf Photosynthetic Rate and Stomatal Characters under High Temperature

Zhang Zhiwei,Li Xiaojing,Bai Jinrui,Chen shuai   

  1. College of Agronomy,Inner Mongolia Agricultural University,Hohhot 010019,Inner Mongolia,China
  • Received:2016-07-20 Revised:2016-08-14 Online:2016-10-15 Published:2018-08-26

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摘要：

探索高温条件下,施加CO2对黄瓜叶片的光合速率、气孔长度、气孔宽度、气孔密度、气孔开张比和气孔开度的影响。采用LI-6400测定光合速率,结果显示高温条件下,经CO2处理的14、21、28、35和42d的黄瓜叶片光合速率显著增加,分别比常温处理增加30.8%、49.7%、34.5%、32.1%和32.1%。利用软件Motic Images Advanced 2.0分析表明,高温结合CO2处理后黄瓜气孔长度、宽度、密度、开张比和开度均显著增加,在处理35、28、42、14和28d时分别比常温处理增加了36.4%、38.8%、37.7%、30.2%和36.9%。

关键词: 黄瓜, 光合速率, 气孔, 高温, CO2

Abstract:

The effects of CO2 on leaf photosynthetic rate, stomatal length and width, stomatal density, stomatal opening ratio and stomatal aperture under high temperature were studied in cucumber. LI-6400 was used to test cucumber photosynthetic rate. The result showed that compared with room temperature, cucumber photosynthetic rate was increased significantly to 30.8%, 49.7%, 34.5%, 32.1% and 32.1% respectively, at 14, 21, 28, 35 and 42 days by treating with high temperature and CO2. By Motic Images Advanced 2.0, cucumber stomatal length and width, stomatal density, stomatal opening ratio and stomatal aperture were all increased significantly by treating with CO2 and high temperature. Compared with treatment of room temperature, they were increased by 36.4%, 38.8%, 37.7%, 30.2% and 36.9% at 35, 28, 42, 14 and 28 days, respectively.

Key words: Cucumber, Photosynthetic rate, Stoma, High temperature, CO2

图1

不同处理环境温度日变化"

图2

不同处理环境CO2浓度日变化"

图3

高温、CO2处理对黄瓜叶片光合速率的影响 不同字母表示处理间差异显著（P<0.05）"

图4

高温、CO2处理对黄瓜气孔长度的影响"

图5

高温、CO2处理对黄瓜气孔宽度的影响"

图6

高温、CO2处理对黄瓜气孔密度的影响"

图7

高温、CO2处理对黄瓜气孔开张比的影响"

图8

高温、CO2处理对黄瓜气孔开张度的影响"

[1] 曹庆杰, 孙权, 李建设 , 等. 不同施氮量对设施黄瓜生长及产量的影响. 北方园艺, 2010(8):1-4.
doi: 10.11937/bfyy.201008001
[2] 卢育华, 申育梅, 陈利平 . 黄瓜单个叶片光合特性研究. 园艺学报, 1994,21(1):54-58.
[3] Long S P, Ort D R . More than taking the heat:crops and global change. Current Opinion in Plant Biology, 2010,13:241-248.
doi: 10.1016/j.pbi.2010.04.008 pmid: 20494611
[4] Baroli I, Price G D, Badger M R , et al. The contribution of photosynthesis to the red light response of stomatal conductance. Plant Physiology, 2008,146:737-747.
doi: 10.1104/pp.107.110924 pmid: 18065555
[5] Kubien D S, Sage R F . The temperature response of photosynthesis in tobacco with reduced amounts of Rubisco. Plant Cell and Environment, 2008,31:407-418.
doi: 10.1111/j.1365-3040.2008.01778.x pmid: 18182015
[6] Crafts-Brandner S J, Salvucci M E . Analyzing the impact of high temperature and CO2 on net photosynthesis:biochemical mechanisms,models and genomics. Field Crops Research, 2004,90(1):75-85.
doi: 10.1016/j.fcr.2004.07.006
[7] Yamori W, Suzuki K, Noguchi K , et al. Effects of Rubisco kinetics and Rubisco activation state on the temperature dependence of the photosynthetic rate in spinach leaves from contrasting growth temperatures. Plant Cell and Environment, 2006,29(8):1659-1670.
doi: 10.1111/pce.2006.29.issue-8
[8] Rockville M D . Exceptional sensitivity of Rubisco activase to thermal denaturation in vitro and vivo. Plant Physiology, 2001,127(3):1053-1064.
doi: 10.1104/pp.010357 pmid: 11706186
[9] Franks P J, Farquhar G D . The mechanical diversity of stomata and its significance in gas-exchange control. Plant Physiology, 2007,143:78-87.
[10] 周海燕, 赵世杰, 孟庆伟 . 高等植物光系统Ⅱ对高温的响应. 生物技术通报, 2006,184(5):8-12.
doi: 10.3969/j.issn.1002-5464.2006.05.002
[11] 赵天宏, 王美玉, 张巍巍 , 等. 大气CO2浓度升高对植物光合作用的影响. 生态环境, 2006,15(5):1096-1100.
[12] Bowes G . Facing the inevitable:plant and atmospheric CO2. Annual Review of Plant Physiology and Plant Molecular Biology, 1993,44:309-332.
doi: 10.1146/annurev.pp.44.060193.001521
[13] Chen G Y, Yong Z H, Liao Y , et al. Photosynthetic acclimation in rice leaves to free-air CO2 enrichment related to both Ribulose-1,5-bisphosphate carboxylation limitation and Ribulose-1,5-bisphosphate regeneration limitation. Plant and Cell Physiology, 2005,46(7):1036-1045.
doi: 10.1093/pcp/pci113
[14] Watanabe C K, Sato S, Yanagisawa S , et al. Effects of elevated CO2 on levels of primary metabolites and transcripts of genes encoding respiratory enzymes and their diurnal patterns in Arabidopsis thaliana:possible relationships with respiratory rates. Plant and Cell Physiology, 2014,55(2):341-357.
doi: 10.1093/pcp/pct185
[15] Song Y, Yu J, Huang B . Elevated CO2-mitigation of high temperature stress associated with maintenance of positive carbon balance and carbohydrate accumulation in Kentucky bluegrass. PloS One, 2014,9(5):e98318.
doi: 10.1371/journal.pone.0098318
[16] Hamilton E W, Heckathorn S A, Joshi P , et al. Interactive effects of elevated CO2 and growth temperature on the tolerance of photosynthesis to acute heat stress in C3 and C4 species. Journal of Integrative Plant Biology, 2008,50(11):1375-1387.
doi: 10.1111/j.1744-7909.2008.00747.x
[17] 蒋跃林, 张庆国, 张仕定 , 等. 小麦光合特性、气孔导度和蒸腾速率对大气CO2浓度升高的响应. 安徽农业大学学报, 2005,32(2):169-173.
[18] 张颖 . 二氧化碳施肥对甜瓜光合特性的影响. 保定:河北农业大学, 2006.
doi: 10.7666/d.y933746
[19] 马博, 崔世茂, 张之为 , 等. 高温CO2加富对温室嫁接黄瓜形态特征净光合速率和Rubisco羧化酶活性的影响. 内蒙古农业大学学报, 2013,3(34):32-39.
[20] 潘璐, 刘杰才, 李晓静 , 等. 高温和加富CO2温室中黄瓜Rubisco活化酶与光合作用的关系. 园艺学报, 2014,41(8):1591-1600.
[21] 胡博, 吕滟, 肖素妮 , 等. 水分胁迫下花生叶片AhNCED1蛋白表达与气孔开度的变化. 植物生理学报, 2012,48(8):815-820.
[22] 杨再强, 谭文, 刘朝霞 , 等. 土壤水分胁迫对设施番茄叶片气孔特性的影响. 生态学杂志, 2015,34(5):1234-1240.
[23] Wang D, Heckathorn S A, Hamilton E W , et al. Effects of CO2 on the tolerance of photosynthesis to heat stress can be affected by photosynthetic pathway and nitrogen. American Journal of Botany, 2014,101(1):34-44.
doi: 10.3732/ajb.1300267
[24] Wang M, Liu H, Dong C, et al.Elevated CO2 enhance photosynthetic efficiency, ions uptake and antioxidant activity of gynura bicolor DC.grown porous-tube nutrient delivery system under simulated microgravity.Plant Biology ( Stuttg), 2016( Epub ahead of print).
[25] Pérez-López U, Miranda-Apodaca J, Mena-Petite A , et al. Barley growth and its underlying components are affected by elevated CO2 and salt concentration. Journal of Plant Growth Regulation, 2013,32(4), 732-744.
doi: 10.1007/s00344-013-9340-x
[26] Wang D, Heckathorn S A, Barua D , et al. Effects of elevated CO2 on the tolerance of photosynthesis to acute heat stress in C3,C4,and CAM species. American Journal of Botany, 2008,95(2):165-176.
doi: 10.3732/ajb.95.2.165
[27] 吴韶辉, 蔡妙珍, 石学根 . 高温对植物叶片光合作用的抑制机理. 现代农业科技, 2010(15):16-18.
[28] 田婧, 郭世荣 . 黄瓜的高温胁迫伤害及其耐热性研究进展. 中国蔬菜, 2012,1(18):43-52.
[29] 李建建, 郁继华, 常雅君 , 等. 高温胁迫对黄瓜幼苗叶片质膜透性及保护酶活性的影响. 长江蔬菜, 2007,7(9):59-61.
[30] Chater C, Peng K, Movahedi M , et al. Elevated CO2-induced responses in stomata require ABA and ABA signaling. Current Biology, 2015,25:2709-2716.
doi: 10.1016/j.cub.2015.09.013
[31] 董发才, 周云, 杨叶 , 等. ABA和H2O2在NaCl诱导的气孔关闭中的作用. 河南大学学报(自然科学版), 2002,32(3):29-32.
doi: 10.3969/j.issn.1003-4978.2002.03.007
[32] 李清明, 刘彬彬, 艾希珍 . CO2浓度倍增对干旱胁迫下黄瓜幼苗膜脂过氧化及抗氧化系统的影响. 生态学报, 2010,30(22):6063-6071.
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