DEP1与NRT1.1B基因的遗传互作对水稻氮素利用的影响

doi:10.16035/j.issn.1001-7283.2021.06.004

摘要/Abstract

摘要：

提高氮素利用效率一直是水稻遗传改良攻关的重点方向。直立穗等位基因dep1及籼稻等位基因nrt1.1b均有利于提高水稻氮素利用效率,因此,阐明DEP1与NRT1.1B基因的互作关系对水稻氮素利用的影响对培育氮高效水稻品种具有重要指导意义。以携带不同DEP1与NRT1.1B基因型组合的重组自交系为供试材料,在低、中及高氮条件下（分别记为LN、MN和HN）,分析了DEP1与NRT1.1B基因间不同的遗传互作方式对水稻氮素利用、产量及其构成因素的影响。结果表明,不同氮素条件下,基因型组合dep1/nrt1.1b具有最大的氮素收获指数;LN条件下,nrt1.1b基因有利于提高氮素利用效率,在MN和HN条件下dep1基因有利于提高氮素利用效率;携带dep1基因株系有效穗数和穗粒数显著提升,其产量均值显著高于其他株系,而NRT1.1B基因对产量无显著影响。

关键词: 水稻, 氮素利用效率, DEP1基因, NRT1.1B基因, 遗传互作

Abstract:

The improvement of nitrogen use efficiency has been the key direction of rice genetic improvement. The erect panicle allele dep1 and indica rice allele nrt1.1b were beneficial to improve the nitrogen use efficiency of rice. Therefore, it was of great significance to clarify the genetic interaction between DEP1 and NRT1.1B genes for the cultivation of nitrogen-efficient rice varieties. In this study, recombinant inbred lines (RILs) carrying different combinations of DEP1 and NRT1.1B genotypes were used as test materials to analyze the effects of genetic interaction between DEP1 and NRT1.1B genes on nitrogen utilization, yield and its components of rice under low (LN), medium (MN) and high nitrogen (HN) conditions. The results showed that under different nitrogen conditions, dep1/nrt1.1b combination had the largest nitrogen harvest index. Under LN condition, the introduction of nrt1.1b gene was conducive to significantly improving nitrogen use efficiency, and the effect of dep1 was more significant under MN and HN conditions. In terms of yield, the average yield of dep1 carrying lines were significantly higher than that of other lines due to the significant increase in effective panicle number and grain number per panicle, while NRT1.1B gene had no significant effect on yield.

Key words: Rice, Nitrogen use efficiency, DEP1 gene, NRT1.1B gene, Genetic interaction

李旭, 付立东, 王宇, 隋鑫, 任海, 吕小红, 马畅, 杜萌, 毛艇. DEP1与NRT1.1B基因的遗传互作对水稻氮素利用的影响[J]. 作物杂志, 2021 (6): 22–27

Li Xu, Fu Lidong, Wang Yu, Sui Xin, Ren Hai, Lü Xiaohong, Ma Chang, Du Meng, Mao Ting. Effects of Genetic Interaction between DEP1 and NRT1.1B on Nitrogen Use in Rice[J]. Crops, 2021(6): 22–27

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基因 Gene	引物名称 Primer name	引物序列（5′-3′） Primer sequence (5′-3′)	片段长度 Fragment size (bp)
DEP1	DEP1-1	DEP1-1-F: AAAGACCAAGGTGCCTCA DEP1-1-R: TGGTTCAACCTCGTCTCATA	466 (dep1 allele) 1091 (DEP1 allele)
NRT1.1B	1nrt/1NRT	NRT1.1B-O-F: GATGGAGGCGATGAGGAAGA NRT1.1B-O-R: GCTGCCAAGAAACACCACAA NRT1.1B-I-F: TCGTGCACAGCCTCCACTTGCTCGACG NRT1.1B-I-R: AGGTCGGCGGCGGAGTCGCCGGCTAT	243 (nrt1.1b allele) 177 (NRT1.1B allele) 368 (CK)

株系 Line	单株有效穗数Effective panicles per plant			穗粒数Grains per panicle
株系 Line	LN	MN	HN	LN	MN	HN
DEP1/NRT1.1B	11±1.31b	13±1.38b	16±1.54b	89±7.80b	109±9.89b	118±10.56b
DEP1/nrt1.1b	12±1.52b	12±1.55b	17±1.59b	90±9.20b	108±11.12b	121±11.81b
dep1/NRT1.1B	14±1.68ab	16±1.92ab	20±2.01ab	108±10.32ab	126±12.11a	135±12.94a
dep1/nrt1.1b	15±1.81a	17±1.89a	21±2.22a	109±11.32a	125±11.35ab	133±10.25ab
株系 Line	千粒重1000-grain weight (g)			产量Yield (kg/hm²)
株系 Line	LN	MN	HN	LN	MN	HN
DEP1/NRT1.1B	27.21±1.45ab	27.33±1.56a	27.32±1.89a	6840±281b	9015±312b	9315±332b
DEP1/nrt1.1b	27.24±1.39a	27.25±1.42ab	27.25±1.45ab	6930±279b	9180±333b	9345±321b
dep1/NRT1.1B	24.23±1.14b	25.31±1.31b	25.12±1.36b	7680±235a	9675±321a	10260±356a
dep1/nrt1.1b	24.21±1.21b	25.12±1.45b	25.31±1.41b	7650±242ab	9630±299ab	10200±371ab