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作物杂志,2021, 第6期: 36–45 doi: 10.16035/j.issn.1001-7283.2021.06.006

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

利用染色体片段置换系定位低温影响水稻萌芽期根长和芽长QTL

余镁霞1(), 邓浩东1(), 谭景艾1, 宋贵廷1, 吴光亮1, 陈利平1, 刘睿琦1, 邹安东1, 贺浩华1,2, 边建民1,2()   

  1. 1作物生理生态与遗传育种教育部/江西省重点实验室,330045,江西南昌
    2江西省水稻高水平工程研究中心,330045,江西南昌
  • 收稿日期:2020-12-14 修回日期:2021-01-01 出版日期:2021-12-15 发布日期:2021-12-16
  • 通讯作者: 边建民
  • 作者简介:余镁霞,研究方向为农学,E-mail： 1873831573@qq.com
  • 基金资助:
    国家重点研发计划(2016YFD0101800);江西省杰出青年人才资助计划项目(20192BCB23010);江西省自然科学基金(20192ACBL20017);江西省教育厅科学技术研究项目(GJJ170241)

Detection of QTL for Root Length and Bud Length at Germination Stage in Low Temperature Using CSSLs in Rice (Oryza sativa)

Yu Meixia1(), Deng Haodong1(), Tan Jing’ai1, Song Guiting1, Wu Guangliang1, Chen Liping1, Liu Ruiqi1, Zhou Andong1, He Haohua1,2, Bian Jianmin1,2()   

  1. 1Key Laboratory of Crop Physiology, Ecology and Genetic Breeding (Jiangxi Agricultural University), Ministry of Education/Jiangxi Province, Nanchang 330045, Jiangxi, China
    2Jiangxi Super Rice Engineering Technology Research Center, Nanchang 330045, Jiangxi, China
  • Received:2020-12-14 Revised:2021-01-01 Online:2021-12-15 Published:2021-12-16
  • Contact: Bian Jianmin

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摘要：

根和芽的正常生长和发育对保障水稻产量有重要作用。为探究在低温条件下影响幼芽期根长和芽长的QTL,以9311（受体）和日本晴（供体）染色体片段置换系群体为材料,将萌发种子置于15℃低温处理7d,然后在25℃下恢复生长7d,连续测量根长和芽长,以25℃下根长和芽长作为对照,定位低温条件下影响根长和芽长的QTL。15℃条件下,定位到9个根长QTL,贡献率为8.65%~24.35%,其中qRL15T7.2和qRL15T10.3在根生长不同时期被重复检测到;定位到9个影响芽长的QTL,贡献率为1.73%~28.89%,其中qBL15T6、qBL15T7、qBL15T9和qBL15T10被重复检测到。25℃条件下,定位到9个根长QTL,贡献率为1.73%~28.89%,qRL25T7、qRL25T9.3、qRL25T10和qRL25T12在根生长不同时期被重复检测到;定位到5个影响芽长QTL,贡献率为0.58%~38.26%,qBL25T3.3在芽生长不同时期被重复检测到。其中,第2、7、9和10号染色体上4个Bin标记区域存在既控制根长又控制芽长的QTL。通过比较发现,15℃下定位到的根长或芽长QTL与25℃定位到的根长或芽长QTL均不相同,表明低温条件下控制水稻幼芽期根和芽生长的遗传机制可能与正常温度下不同。

关键词: 水稻, 根长, 芽长, QTL, 染色体片段置换系

Abstract:

The growth and development of roots and shoots have an important influence on yield. In order to explore the quantitative trait loci (QTL) affecting root length and bud length in bud bursting period under different temperature treatments, the chromosome segment substitution lines (CSSLs) population of 9311 (recipient)/Nipponbare (donor) used as the experimental materials. The germinated seeds were treated at 15℃ for seven days and then recovery grew at 25℃ for seven days. During the recovered period, 10 seeds were randomly selected to measure root length and bud length. After the treatments, the QTL associated with root length and bud length were detected by the CSSLs population. Under 15℃ treatments, nine QTLs affecting root length were detected, the range of contribution rates was 8.65%-24.35%. qRL15T7.2, and qRL15T10.3 were repeatedly detected at different periods of root growth; nine QTL affecting the bud length at 15℃ were detected, and the explained phenotypic variations were from 1.73% to 28.89%, qBL15T6, qBL15T7, qBL15T9, and qBL15T10 were repeatedly detected at different periods of bud growth; Under 25℃ treatments, nine QTL affecting the root length were located, the range of contribution rates was 1.73%-28.89%, qRL25T7, qRL25T9.3, qRL25T10, and qRL25T12 were repeatedly detected; five QTL affecting bud length were detected, and the contribution rates were 0.58%-38.26%, only qBL25T3.3 was repeatedly detected at different periods of bud growth. Four recombination bin markers were located on chromosomes 2, 7, 9, 10, also affected different traits at the same temperature or different traits at different temperatures. The results showed that the QTL affecting root length and bud length were different under different treatments. The results suggest that the genetic mechanism of controlling the growth of roots and buds at the bud stage of rice under low temperature may be different from under normal temperature.

Key words: Rice, Root length, Bud length, QTL, CSSLs

表1

染色体标记数及bin间遗传距离

染色体
Chromosome		 标记数目
Number
of marker		 平均距离
Mean
distance		 最小距离
Minimum
distance		 最大距离
Maximum
distance
1 68 0.64 0.10 3.40
2 64 0.56 0.10 2.90
3 59 0.62 0.10 3.45
4 69 0.52 0.10 4.70
5 47 0.64 0.10 3.60
6 37 0.82 0.10 12.85
7 52 0.57 0.10 2.30
8 34 0.84 0.15 8.90
9 33 0.68 0.15 1.85
10 59 0.40 0.10 1.00
11 76 0.38 0.10 2.50
12 57 0.47 0.10 2.95

图1

染色体片段置换系图谱基因型图示 黑色代表日本晴基因型,白色代表9311基因型

表2

亲本及CSSL群体在2个温度条件下根长和芽长表型分析

温度
Temperature		 性状
Trait		 生长天数
Growth
day (d)		 亲本Parents CSSL群体CSSL population
9311
(cm)		 日本晴
Nipponbare (cm)		 均值
Mean (cm)		 标准误
Standard error		 偏度
Skewness		 峰度
Kurtosis		 最小值
Minimum (cm)		 最大值
Maximum (cm)
15°C 根长 1 2.27 1.62** 2.54 1.03 0.55 -0.38 0.58 4.90
2 2.69 2.00* 3.57 1.12 0.27 -0.78 1.18 5.97
3 2.95 2.43 4.33 1.23 0.02 -0.67 1.23 6.93
4 2.87 2.33 4.87 1.28 -0.13 -0.60 1.46 7.81
5 2.70 2.43 5.34 1.28 -0.68 1.54 0.00 8.08
6 2.60 2.33 5.89 1.33 -1.06 2.46 0.00 8.48
7 2.73 2.51 6.21 1.39 -1.12 2.74 0.00 9.41
芽长 1 2.45 2.01 1.66 0.59 0.95 -0.37 0.90 3.16
2 3.07 3.08 2.92 0.48 -0.16 -0.11 1.67 3.88
3 3.38 3.40 3.93 0.46 -1.39 3.55 2.05 4.87
4 3.59 3.46 4.50 0.48 -1.53 5.40 2.41 5.60
5 3.67 4.29 4.75 0.63 -4.01 27.71 0.00 5.85
6 3.69 5.25** 5.04 0.71 -2.87 23.19 0.00 7.66
7 4.13 5.53** 5.56 0.91 -1.37 12.57 0.00 9.07
25°C 根长 1 0.43 0.00** 0.40 0.33 0.32 -1.21 0.00 1.07
2 1.40 0.28** 1.83 0.90 0.05 -1.11 0.20 3.62
3 2.74 1.61** 3.18 1.17 -0.06 -0.92 0.92 5.70
4 3.75 2.49** 3.96 1.14 -0.31 -0.79 1.47 6.21
5 4.50 3.88 4.75 1.22 -0.44 -0.77 2.08 6.94
6 5.49 4.60 5.24 1.17 -0.62 -0.22 1.87 7.28
7 6.26 5.71 5.74 1.13 -0.46 -0.42 2.64 7.81
芽长 1 0.20 0.00 0.25 0.15 4.44 25.49 0.08 1.18
2 0.82 0.25 0.81 0.29 2.48 10.67 0.39 2.33
3 1.40 0.73* 1.58 0.43 0.68 0.32 0.90 3.06
4 2.44 1.02** 2.29 0.54 0.27 -0.69 1.19 3.77
5 3.09 2.06** 3.14 0.63 0.44 0.92 1.62 5.55
6 3.72 2.76** 3.76 0.61 -0.08 -0.48 2.26 5.12
7 4.02 3.68 4.19 0.56 -0.55 0.27 2.43 5.29

图2

15℃和25℃处理下CSSL群体表型分布

表3

CSSL群体定位2个温度条件下幼芽生长不同时期影响根长和芽长的QTL

温度
Temperature		 性状
Trait		 生长天数
Growth day (d)		 染色体
Chromosome		 QTL 连锁标记
Linkage marker		 LOD值
LOD value		 加性效应
Additive effect		 贡献率
Contribution rate (%)
15°C 根长 1 2 qRL15T2.1 chr2-bin113 3.07 0.69 11.42
7 7 qRL15T7.1 chr7-bin385 3.23 -1.15 8.66
4 qRL15T7.2 chr7-bin387 2.66 -1.03 8.68
6 3.84 -1.25 11.92
3 qRL15T7.3 chr7-bin393 3.01 -0.75 9.58
1 10 qRL15T10.1 chr10-bin501 2.75 -0.61 10.17
4 qRL15T10.2 chr10-bin504 4.08 -1.16 13.73
3 qRL15T10.3 chr10-bin505 4.41 -1.28 14.46
6 5.36 -1.50 17.17
7 qRL15T10.4 chr10-bin507 8.27 -2.21 24.36
5 qRL15T10.5 chr10-bin510 4.12 -1.12 15.61
芽长 1 1 qBL15T1 chr1-bin11 5.24 0.66 17.05
1 2 qBL15T2 chr2-bin113 3.59 0.41 11.08
3 3 qBL15T3.1 chr3-bin135 4.51 -0.86 13.82
3 qBL15T3.2 chr3-bin154 2.79 -0.47 8.22
5 6 qBL15T6 chr6-bin339 25.36 2.31 19.50
6 16.24 1.92 14.72
2 7 qBL15T7 chr7-bin393 2.70 -0.30 10.60
3 5.55 -0.35 17.37
4 3.67 -0.35 13.69
5 8 qBL15T8 chr8-bin425 3.68 0.69 1.73
6 9 qBL15T9 chr9-bin448 3.27 0.61 2.22
7 2.96 0.95 11.54
4 10 qBL15T10 chr10-bin507 2.57 -0.43 8.55
5 31.09 -2.22 26.92
6 27.73 -2.19 28.90
25°C 根长 4 1 qRL25T1 chr1-bin52 2.93 -0.92 6.03
5 7 qRL25T7 chr7-bin345 3.07 -0.73 6.04
6 3.56 -0.94 10.76
5 8 qRL25T8.1 chr8-bin415 2.56 -1.06 5.22
4 qRL25T8.2 chr8-bin430 5.13 -1.25 11.08
6 9 qRL25T9.1 chr9-bin439 2.83 -0.72 8.43
5 qRL25T9.2 chr9-bin448 4.37 -1.16 9.24
2 qRL25T9.3 chr9-bin459 3.32 -0.68 10.24
3 3.31 -0.93 12.82
4 5.84 -0.96 12.80
5 4.28 -0.82 9.03
4 10 qRL25T10 chr10-bin495 4.47 -0.83 9.51
5 5.84 -0.98 12.74
6 3.10 -0.81 9.28
4 12 qRL25T12 chr12-bin599 3.11 -0.95 6.43
5 4.47 -1.18 9.48
芽长 1 3 qBL25T3.1 chr3-bin136 45.91 -0.46 29.14
1 qBL25T3.2 chr3-bin138 51.60 0.47 38.26
1 qBL25T3.3 chr3-bin147 21.86 0.46 7.53
2 7.17 0.77 25.74
3 2.70 0.75 10.59
5 qBL25T3.4 chr3-bin186 3.52 1.22 13.60
1 11 qBL25T11 chr11-bin596 2.59 0.06 0.58

图3

CSSL群体定位的2个温度处理后影响根长和芽长QTL染色体分布

表4

对性状有多效性的染色体标记对应区域

染色体
Chromosome		 连锁标记
Linkage
marker		 物理位置
Physical location
(Mb)		 影响性状Affected trait
15℃ 25℃
2 bin113 19.75~20.75 根长、芽长
7 bin393 28.25~28.70 根长、芽长
9 bin448 11.15~12.45 芽长 根长
10 bin507 15.75~17.20 根长、芽长
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