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作物杂志,2026, 第3期: 185–196 doi: 10.16035/j.issn.1001-7283.2026.03.024

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

花生FKBP基因家族成员的鉴定及表达分析

李洁1,2(), 刘聪聪1, 张莹莹1, 董晓月1, 杨宜红1, 马金娜1, 谷献锋1   

  1. 1 濮阳市农林科学院， 457000， 河南濮阳
    2 福建农林大学农学院， 350002， 福建福州
  • 收稿日期:2025-09-11 修回日期:2025-12-30 出版日期:2026-06-15 发布日期:2026-06-17
  • 作者简介:李洁，主要从事花生遗传研究，E-mail：lijie19861223@126.com
  • 基金资助:
    濮阳市重大科技专项(240210);河南省农业良种联合攻关项目(2022010302)

Identification and Expression Analysis of FKBP Gene Family Members in Peanut

Li Jie1,2(), Liu Congcong1, Zhang Yingying1, Dong Xiaoyue1, Yang Yihong1, Ma Jinna1, Gu Xianfeng1   

  1. 1 Puyang Academy of Agriculture and Forestry Sciences, Puyang 457000, Henan, China
    2 College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
  • Received:2025-09-11 Revised:2025-12-30 Online:2026-06-15 Published:2026-06-17

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摘要：

植物FKBP基因家族是一个功能多样的关键蛋白家族，本文分别从理化性状、保守结构、系统进化和组织表达模式等方面对花生FKBP基因家族进行深入研究。结果表明，56个AhFKBP基因不均匀分布在20条花生染色体上，亚细胞定位预测发现大部分基因定位于叶绿体和细胞核。系统进化和保守结构分析结果表明，AhFKBP基因可分为7个亚家族，同一亚家族成员具有相似的保守基序、内含子和编码序列（CDS）等，且AhFKBP基因家族与拟南芥和黄瓜存在多个同源基因，亲缘关系较近，其次是水稻和小麦。AhFKBP基因表达分析结果表明，不同基因在不同组织和胁迫环境下表达量存在显著差异。对AhFKBP基因家族启动子顺式作用元件分析发现，该基因家族启动子富含光反应、逆境胁迫等元件，推测AhFKBP基因家族参与花生光合适应性和多种胁迫响应过程。

关键词: 花生（Arachis hypogaea L.）, FKBPs, 基因家族, 结构分析, 表达分析

Abstract:

The FKBP gene family is a functionally diverse and important protein family in plants. In this study, the FKBP gene family in peanut (Arachis hypogaea L.) was systematically analyzed in terms of physicochemical properties, conserved structures, phylogenetic tree, and tissue-specific expression patterns. The results showed that a total of 56 AhFKBP genes were unevenly distributed across 20 peanut chromosomes. Subcellular localization prediction indicated that most genes are localized in the chloroplast and nucleus. Phylogenetic tree and conserved structure analyses classified the AhFKBP genes into seven subfamilies, with members within the same subfamily sharing similar conserved motifs, exon-intron structures, and coding sequence (CDS), and AhFKBP genes share closer evolutionary relationships with those in Arabidopsis and cucumber, followed by rice and wheat. Expression analysis demonstrated that AhFKBP genes exhibit significant variation in expression levels across different tissues and under various stress conditions. Promoter cis-acting element analysis showed that gene family are enriched with regulatory elements related to light responsiveness and stress conditions, suggesting their involvement in photosynthetic adaptation and multiple stress response pathways in peanut.

Key words: Peanut (Arachis hypogaea L.), FKBPs, Gene family, Structural analysis, Expression analysis

表1

AhFKBP基因家族成员及其理化性质

基因名称
Gene
name		 基因位点
Gene locus		 CDS长度
CDS length
(bp)		 氨基酸数
Number of
amino acids		 分子量
Molecular
weight
(kDa)		 等电点
Isoelectric
point		 跨膜结构域
Transmembrane
domain		 不稳定性
系数
Instability
index		 脂肪
系数
Fat
index		 平均疏水性
Average
hydrophobicity		 亚细胞定位预测
Subcellular
localization
prediction
AhFKBP1 AH01G03290.1 648 215 24.00 8.44 0 52.61 95.63 -0.078 叶绿体
AhFKBP2 AH01G12790.1 1872 623 70.10 5.33 0 42.43 74.00 -0.581 细胞质
AhFKBP3 AH01G16560.1 546 181 20.45 7.65 0 43.44 94.36 0.155 叶绿体
AhFKBP4 AH02G05330.1 729 242 27.24 8.64 0 60.07 76.49 -0.419 叶绿体
AhFKBP5 AH03G34340.1 705 234 25.08 8.10 0 27.94 90.00 0.021 线粒体
AhFKBP6 AH03G43820.1 732 243 26.30 8.99 0 48.70 81.77 -0.256 叶绿体
AhFKBP7 AH03G46540.1 2841 946 105.08 5.51 0 40.18 91.31 -0.160 细胞核
AhFKBP8 AH03G47450.1 633 210 22.25 9.24 0 45.98 86.81 -0.065 叶绿体外膜
AhFKBP9 AH04G10810.1 474 157 17.82 7.71 0 39.81 88.73 -0.292 细胞核
AhFKBP10 AH04G13950.1 708 235 25.65 8.26 1 57.12 83.40 -0.105 叶绿体
AhFKBP11 AH04G16570.1 444 147 15.79 6.83 0 31.83 96.12 -0.065 细胞外间隙
AhFKBP12 AH04G16590.1 1653 550 61.55 5.18 0 29.70 77.09 -0.629 细胞质
AhFKBP13 AH04G16590.2 1653 550 61.55 5.18 0 29.70 77.09 -0.629 细胞质
AhFKBP14 AH05G00870.1 699 232 25.26 8.95 0 38.62 78.58 -0.241 叶绿体
AhFKBP15 AH05G15850.1 1482 493 54.74 5.21 0 52.79 57.89 -1.059 细胞核
AhFKBP16 AH05G27750.1 381 126 14.09 4.83 0 28.70 80.32 -0.228 细胞核
AhFKBP17 AH05G28070.1 426 141 15.58 7.60 0 30.39 76.03 -0.383 细胞外间隙
AhFKBP18 AH06G10440.1 1065 354 40.54 6.18 1 43.54 76.07 -0.595 细胞核
AhFKBP19 AH06G10440.2 1095 364 41.59 5.87 1 40.64 75.33 -0.579 细胞核
AhFKBP20 AH06G19370.1 1644 547 61.66 5.63 0 30.55 76.11 -0.588 细胞质
AhFKBP21 AH06G25040.1 507 168 18.60 6.92 0 29.17 73.04 -0.655 细胞核
AhFKBP22 AH07G09740.1 561 186 19.89 6.22 0 32.84 76.67 -0.439 细胞核
AhFKBP23 AH09G07580.1 1728 575 64.15 5.26 0 27.59 77.51 -0.595 细胞质
AhFKBP24 AH10G05330.1 339 112 12.00 6.55 0 30.15 67.77 -0.346 细胞外间隙
AhFKBP25 AH11G02960.1 570 189 20.53 5.02 0 21.97 76.30 -0.472 细胞质
AhFKBP26 AH11G05460.1 654 217 24.20 8.76 0 51.33 95.21 -0.093 叶绿体
AhFKBP27 AH11G12840.1 1872 623 70.19 5.33 0 43.67 73.53 -0.594 细胞质
AhFKBP28 AH11G18990.1 780 259 28.90 8.52 0 37.97 72.01 -0.623 细胞质
AhFKBP29 AH12G06190.1 729 242 27.22 8.43 0 59.13 76.49 -0.417 叶绿体
AhFKBP30 AH12G35990.1 531 176 18.64 7.74 0 41.96 88.52 -0.103 细胞核
AhFKBP31 AH13G06390.1 744 247 26.26 6.38 0 46.54 89.96 -0.028 细胞质
AhFKBP32 AH13G19950.1 354 117 12.99 4.92 0 33.95 111.62 0.279 细胞外间隙
AhFKBP33 AH13G20760.1 1158 385 43.23 7.51 0 36.78 88.70 -0.412 细胞质
AhFKBP34 AH13G38140.1 627 208 22.18 8.78 0 28.32 82.07 -0.078 线粒体
AhFKBP35 AH13G38140.2 627 208 22.18 8.78 0 28.32 82.07 -0.078 线粒体
AhFKBP36 AH13G46550.1 732 243 26.26 8.65 0 50.28 80.58 -0.246 叶绿体
AhFKBP37 AH13G50150.1 636 211 22.41 9.23 0 44.84 86.40 -0.033 叶绿体
AhFKBP38 AH13G52010.1 1731 576 64.43 5.20 0 30.94 74.79 -0.653 细胞质
AhFKBP39 AH14G15180.1 663 220 24.47 5.54 0 19.82 76.68 -0.564 细胞质
AhFKBP40 AH14G15690.1 1731 576 64.53 5.28 0 30.89 76.48 -0.647 细胞质
AhFKBP41 AH14G15690.2 1455 484 53.88 5.20 0 26.92 73.26 -0.651 细胞质
AhFKBP42 AH14G19580.1 747 248 27.34 9.08 1 57.84 84.11 -0.121 叶绿体
AhFKBP43 AH14G39590.1 639 212 23.51 8.41 0 42.47 83.21 -0.267 细胞外间隙
AhFKBP44 AH14G39590.2 696 231 25.47 7.55 0 42.93 81.47 -0.216 细胞外间隙
AhFKBP45 AH15G07050.1 1485 494 54.82 5.25 0 50.36 58.56 -1.049 细胞核
AhFKBP46 AH15G26530.1 963 320 34.89 5.67 0 42.65 86.22 -0.093 细胞器膜
AhFKBP47 AH16G16120.1 1065 354 40.52 7.03 1 44.10 77.43 -0.587 细胞核
AhFKBP48 AH16G16120.2 783 260 29.49 5.04 0 37.19 73.50 -0.466 细胞质
AhFKBP49 AH16G24090.1 1644 547 61.73 5.41 0 29.80 77.17 -0.575 细胞质
AhFKBP50 AH16G28470.1 759 252 27.68 8.45 0 35.83 81.59 -0.354 细胞质
AhFKBP51 AH16G39090.1 1698 565 62.98 5.04 0 30.30 83.65 -0.485 细胞质
AhFKBP52 AH16G42920.1 1776 591 65.60 5.09 0 29.28 83.27 -0.494 细胞质
AhFKBP53 AH17G08750.1 561 186 19.94 6.22 0 32.23 77.20 -0.439 细胞核
AhFKBP54 AH17G15830.1 534 177 19.31 4.88 0 23.64 81.41 -0.412 细胞质
AhFKBP55 AH19G10630.1 1728 575 64.03 5.21 0 27.19 77.34 -0.598 细胞质
AhFKBP56 AH20G08260.1 339 112 12.01 6.55 0 30.91 66.88 -0.370 细胞外间隙

图1

花生、水稻、小麦、黄瓜、拟南芥中FKBP基因家族系统进化树

图2

AhFKBP家族保守基序和基因结构分析

图3

AhFKBP蛋白相互作用网络预测

图4

AhFKBP基因的染色体定位

图5

花生FKBP基因的共线性分析

图6

花生FKBP基因启动子区顺式作用元件预测和量化分析

图7

FKBP基因家族在花生不同组织及不同处理下表达模式分析

图8

GO分析和KEGG通路分析

[1] Zhang H M, Zhu J H, Gong Z Z, et al. Abiotic stress responses in plants. Nature Reviews Genetics, 2022, 23:104-119.
doi: 10.1038/s41576-021-00413-0
[2] Olejnik P, Madrzak C J, Nuc K, et al. Cyclophilins and their functions in abiotic stress and plant-microbe interactions. Biomolecules, 2021, 11(9):1390.
doi: 10.3390/biom11091390
[3] Khan S A, Li M Z, Wang S M, et al. Revisiting the role of plant transcription factors in the battle against abiotic stress. International Journal of Molecular Sciences, 2018, 19(6):1634.
doi: 10.3390/ijms19061634
[4] Romano P, He Z, Luan S, et al. Introducing immunophilins. From organ transplantation to plant biology. Plant Physiology, 2004, 134(4):1241-1243.
doi: 10.1104/pp.103.900108
[5] Galat A. Peptidylprolyl cis/trans isomerases (immunophilins): biological diversity-targets-functions. Current Topics in Medicinal Chemistry, 2003, 3(12):1315-1347.
doi: 10.2174/1568026033451862
[6] Fruman D A, Burakoff S J, Bierer B E, et al. Immunophilins in protein folding and immunosuppression. FASEB Journal, 1994, 8 (6):391-400.
pmid: 7513288
[7] Schreiber S L. Chemistry and biology of the immunophilins and their immunosuppressive ligands. Science, 1991, 251(4991):283-287.
pmid: 1702904
[8] Bierer B E, Patricia K S, Thomas J W, et al. Probing immunosuppressant action with a nonnatural immunophilin ligand. Science, 1990, 250(4980):556-559.
pmid: 1700475
[9] Tropschug M, Wachter E, Mayer S, et al. Isolation and sequence of an FK506-binding protein from N.crassa which catalyses protein folding. Nature, 1990, 346(6285):674-677.
doi: 10.1038/346674a0
[10] Patterson C E, Schaub T, Coleman E J, et al. Developmental regulation of FKBP65: an ER-localized extracellular matrix binding-protein. Molecular Biology of the Cell, 2000, 11(11):3925-3935.
pmid: 11071917
[11] He Z Y, Li L G, Luan S, et al. Immunophilins and parvulins. superfamily of peptidyl prolyl isomerases in Arabidopsis. Plant Physiology, 2004, 134(4):1248-1267.
pmid: 15047905
[12] Gollan P J, Bhave M. Genome-wide analysis of genes encoding FK506-binding proteins in rice. Plant Molecular Biology, 2010, 72(1/2):1-16.
doi: 10.1007/s11103-009-9547-1
[13] Yu Y L, Zhang H, Li W, et al. Genome-wide analysis and environmental response profiling of the FK506-binding protein gene family in maize (Zea mays L.). Gene, 2012, 498(2):212-222.
doi: 10.1016/j.gene.2012.01.094
[14] Leng X P, Liu D, Zhao M Z, et al. Genome-wide identification and analysis of FK506-binding protein family gene family in strawberry (Fragaria×ananassa). Gene, 2014, 534(2):390-399.
doi: 10.1016/j.gene.2013.08.056
[15] Zhang Y P, Han J, Liu D, et al. Genome-wide identification and analysis of FK506-binding protein gene family in peach (Prunus persica). Gene, 2014, 536(2):416-424.
doi: 10.1016/j.gene.2013.10.059 pmid: 24342662
[16] 晁金泉, 杨署光, 陈月异, 等. 巴西橡胶树FKBP基因家族成员的鉴定及表达分析. 分子植物育种, 2018, 16(5):1440-1446.
[17] 李玉峰, 黄训文, 卢凤梅, 等. 尼瓦拉野生稻FKBP基因家族的鉴定及功能分析. 现代化农业, 2021, 43(10):29-33.
[18] 王月雪, 母景娇, 耿梓瀚, 等. 大麦FKBP基因家族的鉴定与表达分析. 分子植物育种. (2025-01-24)[2026-02-10]. https://link-cnki-net-s.webvpn.hnagri.org.cn/urlid/46.1068.S.20250124.1309.006.
[19] Kamphausen T, Fanghänel J, Neumann D, et al. Characterization of Arabidopsis thaliana AtFKBP42 that is membrane-bound and interacts with Hsp90. The Plant Journal, 2002, 32(3):263-276.
doi: 10.1046/j.1365-313X.2002.01420.x
[20] Cheung M Y, Auyeung W K, Li K P, et al. A rice immunophilin homolog, OsFKBP12, is a negative regulator of both biotic and abiotic stress responses. International Journal of Molecular Sciences, 2020, 21(22):8791.
doi: 10.3390/ijms21228791
[21] Meiri D, Breiman A. Arabidopsis ROF1 (FKBP62) modulates thermotolerance by interacting with HSP90.1 and affecting the accumulation of HsfA2-regulated sHSPs. The Plant Journal, 2009, 59(3):387-399.
doi: 10.1111/tpj.2009.59.issue-3
[22] Nigam N, Singh A, Sahi C, et al. SUMO-conjugating enzyme (Sce) and FK506-binding protein (FKBP) encoding rice (Oryza sativa L.) genes:genome-wide analysis, expression studies and evidence for their involvement in abiotic stress response. Molecular Genetics and Genomics, 2008, 279(4):371-383.
doi: 10.1007/s00438-008-0318-5 pmid: 18219493
[23] Geisler M, Kolukisaoglu H Ü, Bouchard R, et al. TWISTED DWARF1, a unique plasma membraneanchored immunophilin- like protein, interacts with Arabidopsis multidrug resistance-like transporters AtPGP1 and AtPGP19. Molecular Biology of the Cell, 2003, 14(10):4238-4239.
pmid: 14517332
[24] Roudier F, Gissot L, Beaudoin F, et al. Very-long-chain fatty acids are involved in polar auxin transport and developmental patterning in Arabidopsis. Plant Cell, 2010, 22(2):364-375.
doi: 10.1105/tpc.109.071209
[25] Harrar Y, Bellec Y, Bellini C, et al. Hormonal control of cell proliferation requires PASTICCINO genes. Plant Physiology, 2003, 132(3):1217-1227.
doi: 10.1104/pp.102.019026 pmid: 12857804
[26] Faure J D, Gingerich D, Howell S H, et al. An Arabidopsis immunophilin, AtFKBP12, binds to AtFIP37 (FKBP interacting protein) in an interaction that is disrupted by FK506. The Plant Journal, 1998, 15(6):783-789.
doi: 10.1046/j.1365-313X.1998.00248.x
[27] Yu Y L, Li Y Z, Huang G X, et al. PwHAP5, a CCAAT-binding transcription factor, interacts with PwFKBP12 and plays a role in pollen tube growth orientation in Picea wilsonii. Journal of Experimental Botany, 2011, 62(14):4805-4817.
doi: 10.1093/jxb/err120 pmid: 21784992
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