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作物杂志,2024, 第1期: 80–89 doi: 10.16035/j.issn.1001-7283.2024.01.011

所属专题： 玉米专题

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

玉米WRKY转录因子IIc亚家族分析及其在干旱胁迫下的表达分析

毋莹1,2(), 胡蝶1,2, 李婷1,2, 段乾元1, 韦宁宁1,2, 张兴华1,2, 徐淑兔1,2, 薛吉全1,2()   

  1. 1西北农林科技大学农学院，712100，陕西杨凌
    2农业农村部西北旱区玉米生物学与遗传育种重点实验室，712100，陕西杨凌
  • 收稿日期:2022-09-07 修回日期:2023-05-24 出版日期:2024-02-15 发布日期:2024-02-20
  • 通讯作者: 薛吉全，主要从事玉米遗传育种研究，E-mail：xjq2934@163.com
  • 作者简介:毋莹，研究方向为玉米遗传育种，E-mail：wuying313@nwafu.edu.cn
  • 基金资助:
    国家玉米产业技术体系(CARS-02)

Analysis of WRKY Transcription Factor IIc Subfamily in Maize and Its Expression Profile under Drought

Wu Ying1,2(), Hu Die1,2, Li Ting1,2, Duan Qianyuan1, Wei Ningning1,2, Zhang Xinghua1,2, Xu Shutu1,2, Xue Jiquan1,2()   

  1. 1College of Agronomy, Northwest A & F University, Yangling 712100, Shaanxi, China
    2Key Laboratory of Maize Biology and Genetic Breeding in Arid Area of Northwest Region, Ministry of Agriculture and Rural Affairs, Yangling 712100, Shaanxi, China
  • Received:2022-09-07 Revised:2023-05-24 Online:2024-02-15 Published:2024-02-20
  • Contact: Xue Jiquan

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摘要：

利用生物信息学方法鉴定了玉米WRKY IIc亚族成员，系统全面地分析其理化性质、染色体定位、基因结构、蛋白保守结构域和启动子顺式作用元件；利用已发表的RNA-seq数据，分析ZmWRKY IIc亚家族基因在不同组织部位和不同生长发育时期干旱处理下的表达；同时预测IIc亚家族ZmWRKY蛋白质的互作网络。结果表明，玉米WRKY IIc亚家族有25个成员，其进化相对保守，可在玉米不同组织和不同生长发育时期参与干旱胁迫响应，并且可能在响应干旱胁迫的过程中与MYB、bHLH转录因子以及生长素调节因子存在一定的互作。

关键词: 玉米, WRKY IIc, 家族分析, 干旱, 表达分析

Abstract:

The members of the WRKY IIc subfamily of maize were identified by bioinformatics methods, and their physicochemical properties, chromosomal location, gene structures, protein conserved domains, cis-acting elements in the promoters were comprehensively analyzed. The expression of ZmWRKY IIc subfamily in different tissue parts and different growth and development stages under drought treatment were analyzed by using the published RNA-seq data. Furthermore, the interaction network of ZmWRKY IIc proteins was constructed. The results showed that the WRKY IIc subfamily of maize had 25 members, they were relatively conserved in evolution and could participate in the regulation of drought stress responses in different tissues and stages of growth and development. They may also interact with MYB, bHLH transcription factors and auxin regulators to resist drought stress.

Key words: Maize, WRKY IIc, Family analysis, Drought, Expression analysis

表1

ZmWRKY IIc亚家族基因基本信息

基因名称
Gene name		 蛋白质名称
Protein name		 位置
Position		 可变剪切
Transcript		 氨基酸数
Number of
amino acids		 分子质量
Molecular
weight		 理论等电点
Theoretical
pI		 亚细胞定位
Subcellular
localization		 总平均亲水性
Grand average of
hydropathicity
ZmWRKYIIc-1 Zm00001d033471_P001 Chr1：263620528-263621882 1 99 11 218.69 9.67 细胞核 -1.111
ZmWRKYIIc-2 Zm00001d034073_P002 Chr1：283193730-283197425 2 331 34 511.99 6.00 细胞核 -0.582
ZmWRKYIIc-3 Zm00001d002794_P001 Chr2：22815199-22820094 1 284 30 667.79 8.54 细胞核 -0.425
ZmWRKYIIc-4 Zm00001d007329_P001 Chr2：228732533-228737268 1 220 24 267.47 9.22 细胞核 -1.003
ZmWRKYIIc-5 Zm00001d039531_P001 Chr3：7394664-7395768 1 273 28 090.01 5.83 细胞核 -0.396
ZmWRKYIIc-6 Zm00001d039584_P001 Chr3：8455756-8463632 1 293 31 182.83 9.48 细胞核 -0.670
ZmWRKYIIc-7 Zm00001d041958_P001 Chr3：145661117-145663100 1 337 36 825.13 5.41 细胞核 -0.624
ZmWRKYIIc-8 Zm00001d043663_P001 Chr3：206352165-206353102 1 211 22 647.26 8.33 细胞核 -0.424
ZmWRKYIIc-9 Zm00001d043950_P001 Chr3：214609031-214612175 1 381 40 180.82 6.76 细胞核 -0.627
ZmWRKYIIc-10 Zm00001d044162_P001 Chr3：220824499-220827409 1 352 38 331.29 6.78 细胞核 -0.923
ZmWRKYIIc-11 Zm00001d049173_P001 Chr4：19025176-19028095 1 229 24 730.15 9.57 细胞核 -0.907
ZmWRKYIIc-12 Zm00001d051328_P002 Chr4：154581235-154589610 1 183 19 852.04 12.04 细胞核 -0.338
ZmWRKYIIc-13 Zm00001d013630_P001 Chr5：15863794-15865837 1 140 15 122.52 5.81 细胞核 -0.965
ZmWRKYIIc-14 Zm00001d037607_P001 Chr6：131561417-131562772 1 195 21 165.48 7.74 细胞核 -0.551
ZmWRKYIIc-15 Zm00001d038761_P001 Chr6：163995859-163996782 2 218 23 442.45 9.18 细胞核 -0.520
ZmWRKYIIc-16 Zm00001d038843_P001 Chr6：165655159-165656827 1 221 22 963.36 6.65 细胞核 -0.302
ZmWRKYIIc-17 Zm00001d039032_P001 Chr6：168945257-168948458 1 408 42 835.64 6.50 细胞核 -0.455
ZmWRKYIIc-18 Zm00001d018656_P001 Chr7：2050308-2051829 1 332 34 764.67 6.39 细胞核 -0.405
ZmWRKYIIc-19 Zm00001d008793_P001 Chr8：20528476-20529755 1 229 24 138.91 7.64 细胞核 -0.478
ZmWRKYIIc-20 Zm00001d009939_P001 Chr8：90768544-90769854 1 205 22 045.12 6.51 细胞核 -0.658
ZmWRKYIIc-21 Zm00001d010805_P001 Chr8：128361517-128362582 1 229 24 295.48 8.72 细胞核 -0.448
ZmWRKYIIc-22 Zm00001d011133_P001 Chr8：140010389-140011159 1 196 20 552.60 8.87 细胞核 -0.579
ZmWRKYIIc-23 Zm00001d011413_P001 Chr8：149844919-149847262 1 359 39 228.34 6.30 细胞核 -0.840
ZmWRKYIIc-24 Zm00001d011527_P001 Chr8：153565171-153568905 1 414 43 413.52 6.09 细胞核 -0.512
ZmWRKYIIc-25 Zm00001d012789_P001 Chr8：181008342-181010034 1 227 25 163.23 8.32 细胞核 -0.711

图1

ZmWRKY IIc亚家族基因在染色体的分布（a）及其编码蛋白系统发育树（b）

图2

ZmWRKY IIc亚家族基因结构

图3

玉米ZmWRKY IIc亚家族蛋白的保守结构域及保守基序分析

图4

玉米ZmWRKY IIc亚家族基因启动子顺式作用元件分析

图5

干旱胁迫下ZmWRKYs IIc亚族基因的表达a、b、c分别为3个来源的数据，均为干旱胁迫和正常灌水条件下的不同组织中ZmWRKY IIc亚族基因表达模式分析，以-log2（FPKM）为值，并使用行标准化，d：蓝色和橙色分别代表V2（两叶期）-V3（三叶期）和R1后期（吐丝后期）干旱胁迫下的差异表达基因。

图6

ZmWRKY IIc亚家族蛋白互作网络分析 表示ZmWRKY IIc亚家族蛋白， 表示WRKY其他亚家族蛋白， 表示bHLH家族蛋白， 表示MYB家族蛋白， 表示其他互作蛋白。各节点圆圈大小表示连接度，节点连线粗细表示相关系数绝对值大小。

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