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作物杂志,2019, 第4期: 183–190 doi: 10.16035/j.issn.1001-7283.2019.04.028

• 生理生化·植物营养·栽培耕作 • 上一篇    下一篇

谷子角质合成基因对干旱胁迫的响应

岳琳祺,施卫萍,郭佳晖,郭平毅,郭杰   

  1. 山西农业大学农学院,030801,山西晋中
  • 收稿日期:2019-03-25 修回日期:2019-06-13 出版日期:2019-08-15 发布日期:2019-08-06
  • 通讯作者: 郭平毅,郭杰
  • 作者简介:岳琳祺,在读硕士,主要从事谷子遗传育种研究
  • 基金资助:
    山西省重点研发计划项目(201803D221019-5,2015-TN-09)

Response of Cutin Synthetic Genes of Foxtail Millet to Drought Stress

Yue Linqi,Shi Weiping,Guo Jiahui,Guo Pingyi,Guo Jie   

  1. College of Agronomy, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China
  • Received:2019-03-25 Revised:2019-06-13 Online:2019-08-15 Published:2019-08-06
  • Contact: Pingyi Guo,Jie Guo

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摘要：

干旱是影响谷子产量的重要因素,以抗旱性强的谷子品种赤谷16（M79）及其母本赤谷10号（E1）,父本承谷8号（H1）作为研究材料,采用苗期自然干旱处理并设置对照,在干旱处理第6天（土壤水分含量约为20%）时取叶片进行转录组测序。用ExPASY分析基因的理化性质,GSDS 2.0分析基因结构,MAGA 7.0构建系统进化树,TMHMM Server v. 2.0预测跨膜结构域,SOPMA预测蛋白的二级结构。结果显示8个谷子角质合成基因编码的蛋白均为亲水性蛋白,且均为酸性;经干旱胁迫处理之后,M79、E1和H1中8个基因的表达变化趋势有所不同,Seita.8G128800和Seita.7G197000表达明显上调;8个基因中有4个基因与玉米的基因具有同源性,有3个基因与水稻的基因具有同源性,编码蛋白部分有跨膜结构域。角质合成基因在谷子响应干旱胁迫过程中发挥作用,其调控机理较为复杂。本研究的结果为进一步探讨谷子角质与抗旱的关系提供了一定理论依据。

关键词: 谷子, 角质, 抗旱, 生物信息学分析

Abstract:

Drought is an important influence factor on the yield of foxtail millet. The drought-resistant foxtail millet variety Chigu 16 (M79) and its female parent Chigu 10 (E1) and male parent Chenggu 8 (H1) were used as test materials in this study. Natural drought treatment at the seedling stage was adopted and a control was set up. Leaves were taken off on the 6th day of drought treatment (soil moisture content was about 20%) for transcriptome sequencing. The physiochemical properties of genes were analyzed on ExPASY, and gene structures were detected on GSDS 2.0. A phylogenetic tree was constructed on MAGA 7.0, the transmembrane domain was predicted on TMHMM Server v. 2.0 and the secondary structure of proteins was predicted using SOPMA. It was found that the proteins encoded by the 8 cutin synthetic genes of foxtail millets were all hydrophilic, and the 8 genes were all acidic. After the drought stress treatment, the expressions of the 8 genes changed in different trends among M79, E1 and H1, and Seita.8G128800 and Seita.7G197000 were significantly upregulated. Four of the eight genes were homologous with corn genes, three of the eight genes were homologous with rice genes, and a part of coding proteins had a transmembrane domain. The cutin synthetic genes play a role in the drought response of foxtail millet, but their regulation mechanism is complex. Our findings provide theoretically underlie for further exploration into the relationship between foxtail millet cutin and drought resistance.

Key words: Foxtail millet, Cutin, Drought resistance, Bioinformatics analysis

图1

谷子角质合成基因结构"

图2

谷子角质合成基因所编码蛋白的亲水性和疏水性分析"

表1

谷子角质合成基因的基本信息"

基因名称
Gene name		 基因长度(bp)
Length of gene		 CDS长度(bp)
Length of CDS		 染色体
Chromosome		 氨基酸数目
Number of amino acids		 等电点
PI		 分子量(Da)
Molecular weight
Seita.1G219200 2 276 1 590 1 529 4.83 187 041.49
Seita.8G128800 5 998 1 365 8 454 4.64 499 484.30
Seita.7G197000 1 629 1 629 7 542 4.86 134 851.34
Seita.1G237600 6 547 1 863 1 620 4.67 545 565.01
Seita.2G306800 3 599 1 509 2 502 4.83 299 516.29
Seita.6G138200 4 194 1 755 6 584 4.76 348 222.58
Seita.1G362700 3 031 1 881 1 626 4.75 253 210.31
Seita.6G244000 4 219 1 494 6 497 4.76 351 628.77

图3

干旱处理后M79、H1和E1角质合成基因差异表达聚类热图"

图4

谷子角质合成基因进化树"

图5

谷子角质合成基因的跨膜结构域预测"

表2

谷子角质合成基因蛋白二级结构预测"

蛋白名称
Protein name		 α螺旋
Alpha helix		 β转角
Beta turn		 无规则卷曲
Random coil		 延伸链
Extended strand
Seita.1G219200 51.04 9.26 13.80 25.90
Seita.8G128800 40.62 9.27 31.35 18.76
Seita.7G197000 45.57 11.99 26.01 16.42
Seita.2G306800 50.60 7.97 24.90 16.53
Seita.6G138200 27.05 12.16 35.45 25.34
Seita.1G362700 45.05 7.19 29.39 18.37
Seita.6G244000 43.86 7.65 28.37 20.12
Seita.1G237600 42.26 8.71 28.23 20.81
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