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作物杂志,2020, 第2期: 182–187 doi: 10.16035/j.issn.1001-7283.2020.02.028

• 植物保护 • 上一篇    下一篇

稻瘟病菌杂交后代致病性遗传分析

张晓玉,张亚玲,靳学慧(),闫天雨,赵泽   

  1. 黑龙江八一农垦大学/黑龙江省植物抗性研究中心,163319,黑龙江大庆
  • 收稿日期:2019-09-09 修回日期:2019-11-29 出版日期:2020-04-15 发布日期:2020-04-13
  • 通讯作者: 靳学慧 E-mail:Jxh2686@163.com
  • 作者简介:张晓玉,主要从事植物病理学研究,E-mail：1593091233@qq.com
  • 基金资助:
    黑龙江省农垦总局科技指导项目(HKKYZD190205);黑龙江省农垦总局科技攻关项目(HNK125A-08-06);黑龙江省农垦总局科技攻关项目(HN35-02-02);黑龙江省教育厅项目(12521376);黑龙江八一农垦大学研究生创新项目(YJSCX2019-Y99)

Genetic Analysis of Pathogenicity of Sexual Progeny of Magnaporthe oryzae

Zhang Xiaoyu,Zhang Yaling,Jin Xuehui(),Yan Tianyu,Zhao Ze   

  1. Heilongjiang Bayi Agricultural University/Heilongjiang Resistance of Plant Research Center, Daqing 163319, Heilongjiang, China
  • Received:2019-09-09 Revised:2019-11-29 Online:2020-04-15 Published:2020-04-13
  • Contact: Xuehui Jin E-mail:Jxh2686@163.com

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摘要：

为明确稻瘟病菌杂交后代对水稻致病性的遗传规律,以亲本菌株HLJ6122和KA3杂交产生的后代群体为试验材料,将其接种于14个单基因系抗性水稻品种,对杂交后代进行毒性差异分析。结果表明,64个杂交后代菌株呈现显著的毒性分离,共出现53种毒性类型,无毒性/毒性分别表现为1∶1、1∶3、3∶1、15∶1。菌株KA3分别对IRBLi-F5、IRBLta-K1、IRBL12-M、IRBLta2-Pi持有1个无毒基因;对品种IRBLa-A、IRBLzt-T、IRBL7-W、IRBLkm-Ts、IRBL20-IR24持有2个无毒基因。64个后代菌株对品种IRBLk-Ka、IRBLz-Fu、IRBL19-M、IRBLb-B、IRBLkp-K60无毒性/毒性也符合1∶3,但2个亲本菌株HLJ6122和KA3对供试水稻品种致病性相同,因此难以判断无毒基因的来源。

关键词: 稻瘟病菌, 杂交后代, 无毒基因, 遗传分析

Abstract:

To clarify the difference of avirulence/virulence in sexual progeny of Magnaporthe oryzae. The progeny population produced by the cross of the parental isolates HLJ6122 and KA3 were used as the test material. the virulence of the sexual progeny was analyzed by inoculating it into 14 single line resistant rice varieties. The results suggested that 64 progeny isolates showed significant virulence segregation and 53 virulence types appeared, the segregation ratios of avirulence/virulence were 1:1, 1:3, 3:1 and 15:1 on different rice varieties, respectively. Avirulence and virulence on rice varieties IRBLi-F5, IRBLta-K1, IRBL12-M, IRBLta2-Pi were controlled by a single gene and by 2 genes on varieties IRBLa-A, IRBLzt-T, IRBL7-W, IRBLkm-Ts, IRBL20-IR24. The segregation of avirulence/ virulence on IRBLk-Ka, IRBLz-Fu, IRBL19-M, IRBLb-B and IRBLkp-K60 in the 64 progeny isolates fit 1:3 ratio, but the avirulence and virulence of parental isolates to varieties was the same, it is therefore, difficult to judge the source of avirulence genes. The above results are of great significance to the localization and cloning of avirulence genes of Magnaporthe oryzae.

Key words: Magnaporthe oryzae, Sexual progeny, Avirulence gene, Genetic analysis

表1

KA3与HLJ6122及其64个杂交后代菌株在单基因系水稻品种上的毒性差异"

水稻品种
Rice variety		 抗性基因
Resistance gene		 致病类型Pathotype
KA3 HLJ6122 后代菌株无毒性/毒性比
Progeny isolate avirulence/virulence		 期望比
Expected ratio		 χ2
IRBLa-A Pi-a A V 41∶23 3∶1 3.52
IRBLi-F5 Pi-i A V 35∶29 1∶1 0.39
IRBLk-Ka Pi-k A A 53∶10 3∶1 2.33
IRBLkp-K60 Pi-kp V V 9∶55 1∶3 3.52
IRBLz-Fu Pi-z A A 55∶9 3∶1 3.52
IRBLzt-T Pi-zt A V 38∶21 3∶1 2.99
IRBLta-K1 Pi-ta A V 37∶27 1∶1 1.27
IRBLb-B Pi-b A A 57∶7 15∶1 1.67
IRBL7-W Pi-7(t) A V 21∶41 1∶3 2.15
IRBL12-M Pi-12(t) A V 30∶33 1∶1 0.06
IRBL19-M Pi-19(t) A A 52∶12 3∶1 1.02
IRBLkm-Ts Pi-km A V 12∶51 1∶3 0.89
IRBL20-IR24 Pi-20 A V 21∶43 1∶3 1.69
IRBLta2-Pi Pi-ta2 A V 25∶39 1∶1 2.64

表2

稻瘟病菌HLJ6122与KA3杂交后代在14个水稻品种的毒性分离"

毒性类型
Virulence
type		 水稻品种Rice variety 菌株数量
No. of
isolates
IRBLa-
A		 IRBLi-
F5		 IRBLk-Ka IRBLkp-
K60		 IRBLz-
Fu		 IRBLzt-
T		 IRBLta-
K1		 IRBLb-
B		 IRBL7-
W		 IRBL12-
M		 IRBL19-
M		 IRBLkm-
Ts		 IRBL20-
IR24		 IRBLta2-
Pi
KA3 A A A V A A A A A A A A A A -
HLJ6122 V V A V A V V A V V A V V V -
A1 V V A V A V V A V V A V V V 4
A2 A A A V A A A A V A A V V V 4
A3 V V V V V V V V V V V V V V 3
A4 A A A V A V A A A A A V V A 2
A5 A A A V A V A A V V A V V V 2
A6 V A A V A A A A A A A V V A 2

表3

稻瘟病菌HLJ6122与KA3杂交后代在4个水稻品种的毒性分离"

毒性类型
Virulence
type		 水稻品种Rice variety 菌株数量
No. of
isolates
IRBLk-Ka IRBLz-Fu IRBLb-B IRBL19-M
KA3 A A A A -
HLJ6122 A A A A -
B1 A A A A 43
B2 A A A V 6
B3 V V V V 3
B4 A V A A 3
B5 V A A A 2

表4

稻瘟病菌HLJ6122与KA3杂交后代在9个水稻品种的毒性分离"

毒性类型Virulence type 水稻品种Rice variety 菌株数量
No. of isolates
IRBLa-A IRBLi-F5 IRBLzt-T IRBLta-K1 IRBL7-W IRBL12-M IRBLkm-Ts IRBL20-IR24 IRBLta2-Pi
KA3 A A A A A A A A A -
HLJ6122 V V V V V V V V V -
C1 V V V V V V V V V 12
C2 A A A A V A V V V 5
C3 A A V A V V V V V 2
C4 A A A A V A V A A 2
C5 A A V A A A V V A 2
C6 A A A A V V V A A 2
C7 V A A A A A V V A 2
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