玉米葡萄糖转运蛋白基因ZmGLUT-1表达特征分析及互作预测

doi:10.16035/j.issn.1001-7283.2020.01.005

作物杂志,2020, 第1期: 22–28 doi: 10.16035/j.issn.1001-7283.2020.01.005

• 遗传育种·种质资源·生物技术 • 上一篇下一篇

玉米葡萄糖转运蛋白基因ZmGLUT-1表达特征分析及互作预测

张新¹,曹丽茹^1,²,魏良明¹,张前进¹,周柯¹,王振华¹(),鲁晓民¹()

¹河南省农业科学院粮食作物研究所,450002,河南郑州
²河南农业大学粮食作物协同创新中心,450002,河南郑州

收稿日期:2019-07-10 修回日期:2019-09-29 出版日期:2020-02-15 发布日期:2020-02-23
通讯作者: 王振华,鲁晓民 E-mail:wzh201@126.com;luxiaomin2004@163.com
作者简介:张新,主要从事玉米遗传育种研究,E-mail: zh5733764@126.com
基金资助:
国家玉米产业技术体系(CARS-02-08);优质青贮玉米种质的创新与改良利用(182102110413)

Expression Analysis and Interaction Prediction of Maize Glucose Transporter Gene ZmGLUT-1

Zhang Xin¹,Cao Liru^1,²,Wei Liangming¹,Zhang Qianjin¹,Zhou Ke¹,Wang Zhenhua¹(),Lu Xiaomin¹()

¹Grain Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
²Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, Henan, China

Received:2019-07-10 Revised:2019-09-29 Online:2020-02-15 Published:2020-02-23
Contact: Zhenhua Wang,Xiaomin Lu E-mail:wzh201@126.com;luxiaomin2004@163.com

摘要/Abstract

摘要：

葡萄糖转运蛋白（GLUT1）通过维持细胞膜两侧的葡萄糖浓度来维护细胞的稳定,在植物抗逆境方面起重要作用。从郑36自选系中克隆了1个GLUT1基因,暂时命名为ZmGLUT-1,该基因含有1 263bp的开放阅读框,编码420个氨基酸;对启动子顺式元件分析,发现该基因含有响应逆境胁迫、激素信号传导、光刺激应答等多种结合位点;蛋白序列分析表明,该蛋白属于疏水性蛋白,含有12个跨膜结构域,主要以α螺旋结构存在,亚细胞定位于质膜上;qRT-PCR结果表明该基因属于组成型表达基因,且在叶尖和胚中高表达,同时发现该基因受脱落酸（ABA）和PEG胁迫下调表达,而复水后表达虽有上升但无法达到正常水平;互作蛋白分析发现,或许ZmGLUT-1与互作蛋白构成调控网络,通过催化和跨膜转运糖类、脂质、激素等物质,来参与细胞代谢物的合成与降解,维护细胞的稳定性,以此来维护植物的生长发育。以上研究表明ZmGLUT-1基因与干旱胁迫和ABA诱导相关。

关键词: 玉米, 葡萄糖转运蛋白, 干旱和脱落酸胁迫, 细胞稳定性

Abstract:

Glucose transporter (GLUT1) maintains cell stability by maintaining glucose concentration on both sides of the cell membrane, which plays an important role in plant stress tolerance. In this study, GLUT1 gene (1 263bp) was cloned from the ‘zheng 36’, which was temporarily named ZmGLUT-1 gene. The promoter cis element analysis revealed that the gene contains various binding sites in response to stress, hormone signaling, and photo-stimulation response. InSilico analysis indicates that the protein is a hydrophobic protein with 12 transmembrane domains, mainly in the alpha helix structure, and subcellular localization on the plasma membrane. The result of qRT-PCR indicated that the gene was a constitutively expressed gene and was highly expressed in leaf tips and embryos. It was also found that the gene was down-regulated by abscisic acid (ABA) and PEG treatment, the expression after rehydration increased but could not reach normal level. Mutual protein analysis revealed that perhaps ZmGLUT-1 protein and the interaction proteins constitute a regulatory network. The above studies indicate that ZmGLUT-1 gene is associated with drought stress. These results provide information for further exploration of GLUT1 function and mechanism in plants, and for screening drought-resistant high-quality breeding germplasms.

Key words: Maize, Glucose transporter, Drought and ABA stress, Cell stability

张新,曹丽茹,魏良明,张前进,周柯,王振华,鲁晓民. 玉米葡萄糖转运蛋白基因ZmGLUT-1表达特征分析及互作预测[J]. 作物杂志, 2020 (1): 22–28

Zhang Xin,Cao Liru,Wei Liangming,Zhang Qianjin,Zhou Ke,Wang Zhenhua,Lu Xiaomin. Expression Analysis and Interaction Prediction of Maize Glucose Transporter Gene ZmGLUT-1[J]. Crops, 2020(1): 22–28

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元件Element	来源植物Source plant	位点Site	序列Sequence	功能Function
ABRE	Oryza sativa	1 525	GCCGCGTGGC	cis-acting element involved in the abscisic acid responsiveness
AuxRR-core	Nicotiana tabacum	1 011	GGTCCAT	cis-acting regulatory element involved in auxin responsiveness
CAT-box	Arabidopsis thaliana	1 640	GCCACT	cis-acting regulatory element related to meristem expression
CGTCA-motif	Hordeum vulgare	494	CGTCA	cis-acting regulatory element involved in the MeJA-responsiveness
GATA-motif	Arabidopsis thaliana	1 106	GATAGGA	part of a light responsive element
GT1-motif	Arabidopsis thaliana	24	GGTTAA	light responsive element
I-box	Zea mays	1 285	GGATAAGGTG	part of a light responsive element
O2-site	Zea mays	1 286	GATGATGTGG	cis-acting regulatory element involved in zein metabolism regulation
Sp1	Oryza sativa	1 459	GGGCGG	light responsive element
TGA-element	Brassica oleracea	428	AACGAC	auxin-responsive element
TGACG-motif	Hordeum vulgare	494	TGACG	cis-acting regulatory element involved in the MeJA-responsiveness

基因号 Gene ID	基因描述 Gene description	功能结构域 Function domain	氨基酸 Amino acid	互作系数 Interaction coefficient
GRMZM2G138423	ADP, ATP carrier protein	ADP, ATP transporter on adenylate translocase	328	0.82
GRMZM2G100976	PhotosystemI1	NifU-like domain	266	0.80
GRMZM5G825759	TP synthase B chain	ATP synthase B/B′ CF(0)	216	0.80
GRMZM2G073244	UDP-glycosyltransferase 84A1	Glycosyltransferase_GTB_type	481	0.80
GRMZM2G124321	UvrB/uvrC motif-containing protein	UvrB/uvrC motif	334	0.72

玉米葡萄糖转运蛋白基因ZmGLUT-1表达特征分析及互作预测

Expression Analysis and Interaction Prediction of Maize Glucose Transporter Gene ZmGLUT-1

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