干旱胁迫对盆栽“川丹参1号”生理指标及主要活性成分含量的影响

doi:10.16035/j.issn.1001-7283.2021.01.011

摘要/Abstract

摘要：

采用聚乙二醇（PEG-6000）对盆栽“川丹参1号”（CDS-1）模拟长期干旱胁迫,研究干旱胁迫对其生长情况、生理指标及主要活性成分积累的影响。结果表明,干旱胁迫显著降低CDS-1叶片中叶绿素含量,可溶性蛋白、可溶性糖和脯氨酸含量随干旱程度增加呈上升趋势,干旱胁迫下丙二醛含量显著增加,抗氧化酶（SOD、POD和CAT）活性随干旱胁迫程度增加而增加。活性成分分析表明,干旱胁迫后CDS-1根中酚酸和丹参酮类含量均有所上升,迷迭香酸和丹酚酸A含量在PEG-6000浓度为150g/L时最高,分别为CK的145%和175%,丹参酮类含量在PEG-6000浓度为50g/L时最高,增幅超60%。CDS-1在干旱胁迫下表现出适应性变化,轻度和中度干旱胁迫（PEG-6000浓度为50~100g/L）有利于CDS-1中酚酸和丹参酮类活性成分的积累,在实际生产中可通过科学控水保证和提高丹参药材的品质。

关键词: 川丹参1号, 干旱胁迫, 生理指标, HPLC, 活性成分

Abstract:

In this study, polyethylene glycol (PEG-6000) was used to simulate long-term drought stress on potted 'Chuandanshen 1' (CDS-1) for investigating the effects of drought stress on the growth, chlorophyll content, physiological indicators, and the content of main active components. The results were as follows: Drought stress significantly reduced the chlorophyll content in the leaves. Under drought stress, the contents of soluble protein, soluble sugar and proline in leaves showed a significant increasing trend with the increase of drought. MDA content increased significantly under middle and severe drought stress. Antioxidant enzyme activity increased with the increasing of drought stress. HPLC analysis of active components showed that phenolic acid and tanshinone content in the root increased after treating with 50-150g/L of PEG-6000. The content of rosmarinic acid and salvianolic acid A were the highest when treated with 150g/L, which was 145% and 175% of CK. The content of tanshinones was the highest when treated with 50g/L PEG-6000, and increased more than 60% compared with CK. Overall, the results demonstrated that CDS-1 showed adaptive changes under drought stress and had a certain drought-resistant ability. The activity of antioxidant enzyme and the contents of main active components of CDS-1 increased, which improved its antioxidant and disease-prevention ability to adapt the drought environment. Light and middle drought stress (50-100g/L PEG-6000) are beneficial to the accumulation of phenolic acid and tanshinones in CDS-1. In actual production, scientific control of water can be used to ensure and improve the quality of medicinal material CDS-1.

Key words: Chuandanshen 1, Drought stress, Physiological index, HPLC, Active components

邓婉月, 冷秋彦, 杨在君, 余燕, 吴一超. 干旱胁迫对盆栽“川丹参1号”生理指标及主要活性成分含量的影响[J]. 作物杂志, 2021 (1): 74–81

Deng Wanyue, Leng Qiuyan, Yang Zaijun, Yu Yan, Wu Yichao. Effects of Simulated Drought Stress on the Physiological Indexes and Contents of Active Components of Potted "Chuandanshen 1"[J]. Crops, 2021(1): 74–81

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PEG浓度 PEG concentration(g/L)	水势梯度 Water potential gradient (MPa)	干旱程度 Degree of drought
0 (CK)	0	–
50	-0.1	轻度
100	-0.4	中度
150	-0.5	中度
200	-0.7	重度

化合物Compound	回归方程Regression equation	相关系数Correlation coefficient (r)	线性范围Linear range (mg/mL)
迷迭香酸Rosmarinic acid	Y=4720.4X+30.738	0.9982	0.0025~0.0625
丹酚酸B Salvianolic acid B	Y=13362X+12.023	0.9998	0.0200~0.2000
丹酚酸A Salvianolic acid A	Y=8580.5X+41.569	0.9970	0.0025~0.0625
隐丹参酮Cryptotanshinone	Y=61908X-3.0334	1	0.0005~0.0125
丹参酮ⅠTanshinone Ⅰ	Y=150729X+0.5474	1	0.0005~0.0125
丹参酮ⅡA Tanshinone ⅡA	Y=171690X-0.0514	1	0.00037~0.00555

PEG-6000浓度 PEG-6000 concentration (g/L)	叶绿素含量 Chlorophyll content (mg/g)			叶绿素a/b Chl a/Chl b
PEG-6000浓度 PEG-6000 concentration (g/L)	Chl a	Chl b	合计 Total	叶绿素a/b Chl a/Chl b
0 (CK)	0.88±0.10a	0.49±0.06a	1.37±0.16a	1.82±0.12c
50	0.73±0.04b	0.38±0.02b	1.11±0.06b	1.89±0.09bc
100	0.72±0.04b	0.33±0.03bc	1.05±0.07b	2.15±0.30abc
150	0.70±0.04b	0.31±0.02bc	1.01±0.06b	2.22±0.11ab
200	0.66±0.07b	0.29±0.04c	0.95±0.11b	2.29±0.27a

PEG-6000浓度 PEG-6000 concentration (g/L)	迷迭香酸 Rosmarinic acid	丹酚酸B Salvianolic acid B	丹酚酸A Salvianolic acid A	隐丹参酮 Cryptptanshinone	丹参酮Ⅰ TanshinoneⅠ	丹参酮ⅡA TanshinoneⅡA	总丹参酮 Total tanshinone
0 (CK)	5.54±0.25c	34.44±1.77a	0.53±0.16b	0.05±0.01c	0.14±0.01b	0.19±0.01c	0.38±0.01b
50	6.00±0.31c	31.60±1.31bc	0.54±0.12b	0.09±0.00a	0.23±0.01a	0.32±0.02a	0.64±0.03a
100	7.64±0.26a	30.00±1.48c	0.58±0.09b	0.07±0.01b	0.24±0.02a	0.28±0.02b	0.59±0.01a
150	8.03±0.36a	34.44±1.12a	0.93±0.17a	0.01±0.00d	0.09±0.00c	0.07±0.01d	0.17±0.01c
200	6.84±0.27b	32.68±0.96ab	0.41±0.09b	0.05±0.01c	0.15±0.03b	0.20±0.02c	0.40±0.05b