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作物杂志,2024, 第3期: 192–200 doi: 10.16035/j.issn.1001-7283.2024.03.026

• 生理生化·植物营养·栽培耕作 • 上一篇    下一篇

灌浆期干旱胁迫对不同小麦品种的生理性状与根系生长的影响

薛鑫雨1(), 詹文博1, 陈新宜1, 周瑞祥1, 王永霞2, 薛瑞丽1, 李华1, 汪月霞1(), 李艳2()   

  1. 1河南农业大学生命科学学院，450002，河南郑州
    2河南省农业科学院作物分子育种研究院，450002，河南郑州
  • 收稿日期:2023-02-28 修回日期:2023-03-01 出版日期:2024-06-15 发布日期:2024-06-18
  • 通讯作者: 汪月霞，主要从事小麦抗逆栽培生理学研究，E-mail：yxwang2100@126.com；李艳，主要从事小麦抗逆栽培生理学研究，E-mail：liyanly7812@163.com
  • 作者简介:薛鑫雨，主要从事小麦抗逆生理学研究，E-mail：hengzi594@163.com
  • 基金资助:
    国家自然科学基金(U1704103);河南省自然科学基金(222300420453);财政部和农业农村部：国家现代农业产业技术体系(CARS-03);河南省科技攻关计划(222102110405)

Effects of Drought Stress during Grain Filling Period on Physiological and Root Characteristics of Different Wheat Cultivars

Xue Xinyu1(), Zhan Wenbo1, Chen Xinyi1, Zhou Ruixiang1, Wang Yongxia2, Xue Ruili1, Li Hua1, Wang Yuexia1(), Li Yan2()   

  1. 1College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2Institute of Crops Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
  • Received:2023-02-28 Revised:2023-03-01 Online:2024-06-15 Published:2024-06-18

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摘要：

为明确灌浆期干旱胁迫对不同品种小麦生理特性和根系生长的影响，以高产高抗新品种郑麦1860和对照品种百农207、周麦18为材料，研究了灌浆期干旱处理对小麦根生长和生理性状的影响。结果表明，干旱胁迫下，郑麦1860的净光合速率（Pn）显著高于周麦18，郑麦1860与百农207的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性提高，同时郑麦1860具有更高的根冠比、根尖数以及根表面积。与百农207和周麦18相比，郑麦1860的丙二醛含量在复水后与对照组差异最小。试验结果表明灌浆期干旱胁迫下郑麦1860能维持较高的Pn，稳定籽粒产量。通过提高SOD、CAT和POD活性从而加快谷胱甘肽再生，通过促进还原型谷胱甘肽/氧化型谷胱甘肽升高来减缓膜脂过氧化损伤。并且郑麦1860具有更强的吸水能力，在干旱胁迫下清除多余活性氧的能力更强，从而减少干旱胁迫对膜结构造成的损伤。

关键词: 小麦, 干旱胁迫, 根, 保护酶, 基因表达调控

Abstract:

In order to study the effects of drought stress during grain filling period on physiological and root characteristics among different wheat cultivars, the new variety Zhengmai 1860 with high yield and high resistance, Bainong 207 and Zhoumai 18 were selected as experimental materials. The results showed the net photosynthetic rate (Pn) of Zhengmai 1860 was significantly higher than that of Zhoumai 18, the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in Zhengmai 1860 and Bainong 207 increased under drought stress, meanwhile, Zhengmai 1860 had higher ratio of root to shoot, the number of root tips and root surface. Compared with Bainong 207 and Zhoumai 18, the malonaldehyde (MDA) content of Zhengmai 1860 was less than that of the control group after rehydration. The results also showed that Zhengmai 1860 could maintain a high Pn and stabilize grain yield under drought stress at grain filling stage. By increasing activities of SOD, CAT and POD, it accelerated the regeneration of glutathione and promoted the increase of reduced glutathione/oxidized glutathione to slow down the membrane lipid peroxidation damage. Furthermore, Zhengmai 1860 has stronger water absorption capacity, greater ability to remove excess reactive oxygen species under drought stress, thus reducing the damage of membrane structure caused by drought stress.

Key words: Triticum aestivum L., Drought stress, Root, Protective enzyme, Regulation of gene expression

表1

实时荧光定量PCR引物信息

基因名称Gene name 引物序列Primer sequence (5′-3′)
SOD-Cu/Zn
 F：CGCTCAGAGCCTCCTCTTT
R：CTCCTGGGGTGGAGACAAT
FeSOD
 F：GTCCTACTACGGCCTCACCA
R：ACGTAGTCCTGCTGGTGCTT
MnSOD
 F：CAGAGGGTGCTGCTTTACAA
R：GGTCACAAGAGGGTCCTGAT
CAT
 F：CCATGAGATCAAGGCCATCT
R：ATCTTACATGCTCGGCTTGG
POD
 F：TGGGCATGGGGCTTCTGCA
R：GCGAGGAATGGGGGGTTGATG
Actin
 F：GGAATCCATGAGACCACCTAC
R：GACCCAGACAACTCGCAAC

表2

灌浆期干旱胁迫对不同品种小麦根系生长的影响

处理
Treatment		 品种
Variety		 总根长
Len (cm)		 根表面积
SA (cm2)		 根投影面积
PA (cm2)		 根系平均直径
AvgD (mm)		 总根尖数
NTipS		 分枝数
NForkS
对照CK 百农207 1225.89±3.25d 610.52±6.55c 194.34±2.69b 1.59±0.04a 8848±14d 12 123±25d
郑麦1860 3767.47±7.34b 673.39±2.74b 214.35±4.13ab 0.57±0.02c 19 218±7a 35 726±10a
周麦18 4498.28±7.92a 869.46±9.64a 276.76±6.14a 0.62±0.02c 18 749±11a 39 078±9a
干旱组D 百农207 1917.80±10.66c 472.95±4.44d 150.55±7.35c 0.79±0.03c 12 857±5bc 15 000±23c
郑麦1860 2067.51±15.45c 691.12±5.96b 219.99±9.52ab 1.06±0.06b 14 832±12b 23 143±15b
周麦18 1576.93±8.66d 620.53±4.87c 197.52±6.97b 1.25±0.05ab 9595±40d 14 672±19c

图1

干旱胁迫下不同品种小麦根部表型

表3

干旱胁迫对不同品种小麦生物量、千粒重、产量、穗粒数的影响

处理
Treatment		 品种
Variety		 根干重（g/株）
Root dry weight
(g/plant)		 茎干重（g/株）
Stem dry weight
(g/plant)		 根冠比
Root-shoot
ratio		 千粒重
1000-grain
weight (g)		 穗粒数
Grain number
per spike		 产量
Yield
(kg/hm2)
对照CK 郑麦1860 9.01±0.97a 50.01±6.01a 0.18±0.02ab 50.45±0.93a 50±4b 8208.0±50.1a
百农207 8.15±0.99a 50.09±5.10a 0.16±0.01b 42.11±0.62c 58±3a 8068.5±62.3b
周麦18 6.79±0.68bc 45.26±4.20ab 0.15±0.01b 47.84±0.86b 44±3d 7702.3±71.4c
干旱组D 郑麦1860 8.74±0.70ab 41.60±3.10ab 0.21±0.02ab 42.09±0.81c 47±3c 6847.9±43.6d
百农207 7.85±0.71ab 35.70±2.00c 0.22±0.01a 34.02±0.72e 51±2b 6518.4±72.3e
周麦18 5.10±0.57c 30.50±2.80d 0.17±0.01b 38.28±0.95d 38±3e 6163.1±78.8f

表4

干旱胁迫对不同品种小麦气孔孔径、气孔开度和Pn的影响

处理Treatment 品种Variety 气孔孔径Stomatal aperture size (μm) 气孔开度Stomatal open size (μm) Pn [μmol/(m2·s)]
干旱胁迫7 d
7 d after drought stress		 CK 郑麦1860 0.63±0.04a 0.30±0.02a 26.30±0.54a
百农207 0.54±0.01ab 0.30±0.04a 25.03±0.47b
周麦18 0.55±0.04ab 0.30±0.03a 24.23±0.52c
D 郑麦1860 0.55±0.04ab 0.15±0.01b 23.23±0.26cd
百农207 0.53±0.00b 0.11±0.01c 22.57±0.52d
周麦18 0.53±0.05b 0.06±0.01d 19.10±0.42e
复水后2 d
2 d after rewatering		 CK 郑麦1860 0.62±0.03a 0.22±0.00a 26.30±0.54a
百农207 0.51±0.01c 0.18±0.01b 25.43±0.47b
周麦18 0.66±0.02a 0.17±0.00b 24.30±0.62b
D 郑麦1860 0.50±0.02c 0.19±0.01b 25.40±0.56ab
百农207 0.53±0.02bc 0.13±0.00c 24.57±0.59b
周麦18 0.57±0.02b 0.12±0.00c 21.10±0.57c

图2

干旱胁迫及复水对不同品种小麦旗叶MDA含量和细胞质膜透性的影响 不同小写字母表示处理间显著差异（P < 0.05），下同。

图3

干旱胁迫及复水对不同品种小麦叶片SOD、CAT、POD活性以及GSH、GSSG、GSH/GSSG的影响

图4

干旱胁迫及复水对不同品种小麦旗叶保护酶相关基因表达水平的影响

[1] 郝瑞煊, 孙敏, 任爱霞, 等. 宽幅条播冬小麦水分利用与干物质积累、品质的关系及播种密度的调控研究. 作物杂志, 2022(2):119-126.
[2] Li C N, Li L, Reynolds M, et al. Recognizing the hidden half in wheat: root system attributes associated with drought tolerance. Journal of Experimental Botany, 2021, 72(14):5117-5133.
[3] Fahad S, Bajwa A A, Nazir U, et al. Crop production under drought and heat stress: plant responses and management options. Frontiers in Plant Science, 2017, 8:1147-1162.
doi: 10.3389/fpls.2017.01147 pmid: 28706531
[4] Luo Y L, Li W Q, Huang C, et al. Exogenous abscisic acid coordinating leaf senescence and transport of assimilates into wheat grains under drought stress by regulating hormones homeostasis. The Crop Journal, 2021, 9(4):901-914.
[5] Su R N, Chen L, Wang Z H, et al. Differential response of cuticular wax and photosynthetic capacity by glaucous and non- glaucous wheat cultivars under mild and severe droughts. Plant Physiology and Biochemistry, 2020, 147(6):303-312.
[6] Mathew I, Shimelis H, Shayanowako A I T, et al. Genome-wide association study of drought tolerance and biomass allocation in wheat. PLoS ONE, 2019, 14(12):e0225383
[7] Du H Y, Dong Q Y, Liu H P, et al. Polyamines conjugated to plasma membrane functioned in enhancing the tolerance of cucumber seedlings to osmotic stress via elevating H+-ATPase activity. Plant Physiology and Biochemistry, 2022, 170:64-74.
[8] Ebeed H T, Hassan N M, Aljarain A M. Exogenous applications of polyamines modulate drought responses in wheat through osmolytes accumulation, increasing free polyamine levels and regulation of polyamine biosynthetic genes. Plant Physiology and Biochemistry, 2017, 118:438-448.
doi: S0981-9428(17)30236-X pmid: 28743037
[9] Chen D D, Chai S C, Mcintyre C L, et al. Overexpression of a predominantly root-expressed NAC transcription factor in wheat roots enhances root length, biomass and drought tolerance. Plant Cell Reports, 2018, 37(2):225-237.
doi: 10.1007/s00299-017-2224-y pmid: 29079898
[10] Hu C H, Zeng Q D, Tai L, et al. Interaction between TaNOX7 and TaCDPK 13 contributes to plant fertility and drought tolerance by regulating ROS production. Journal of Agricultural and Food Chemistry, 2020, 68(28):7333-7347.
[11] Song R J, Zhang X C, Feng C J, et al. Exogenous hydrogen promotes germination and seedling establishment of barley under drought stress by mediating the ASA-GSH cycle and sugar metabolism. Journal of Plant Growth Regulation, 2023, 42:2749- 2762.
[12] Abid M, Ali S, Qi L K, et al. Physiological and biochemical changes during drought and recovery periods at tillering and jointing stages in wheat (Triticum aestivum L.). Scientific Reports, 2018, 8(1):4615.
[13] Hassan N, Ebeed H, Aljaarany A. Exogenous application of spermine and putrescine mitigate adversities of drought stress in wheat by protecting membranes and chloroplast ultra-structure. Physiology and Molecular Biology of Plants, 2020, 26(2):233- 245.
doi: 10.1007/s12298-019-00744-7 pmid: 32158131
[14] Kirova E, Pecheva D, Simova-stoilova L. Drought response in winter wheat: protection from oxidative stress and mutagenesis effect. Acta Physiologiae Plantarum, 2021, 43(1):8.
[15] Lin Z, Wang Y L, Cheng L S, et al. Mutual regulation of ROS accumulation and cell autophagy in wheat roots under hypoxia stress. Plant Physiology and Biochemistry, 2021, 158:91-102.
doi: 10.1016/j.plaphy.2020.11.049 pmid: 33302125
[16] Saed-moucheshi A, Sohrabi F, Fasihfar E, et al. Superoxide dismutase (SOD) as a selection criterion for triticale grain yield under drought stress: a comprehensive study on genomics and expression profiling, bioinformatics, heritability, and phenotypic variability. BMC Plant Biology, 2021, 21(1):148.
doi: 10.1186/s12870-021-02919-5 pmid: 33752615
[17] Aliyeva D R, Aydinli L M, Zulfugarov I S, et al. Diurnal changes of the ascorbate-glutathione cycle components in wheat genotypes exposed to drought. Functional Plant Biology, 2020, 47(11):998-1006.
[18] Gasperl A, Balogh E, Boldizsár Á, et al. Comparison of light condition-dependent differences in the accumulation and subcellular localization of glutathione in arabidopsis and wheat. International Journal of Molecular Sciences, 2021, 22(2):607.
[19] Hasanuzzaman M, Nahar K, Rahman A, et al. Exogenous glutathione attenuates lead-induced oxidative stress in wheat by improving antioxidant defense and physiological mechanisms. Journal of Plant Interactions, 2018, 13(1):203-212.
[20] Ghahremani B, Hassannejad S, Alizadeh K, et al. Salicylic acid alleviates oxidative stress and lipid peroxidation caused by clopyralid herbicide in Indian mustard plants. Acta Physiologiae Plantarum, 2022, 44(4):49.
[21] 刘建新, 刘瑞瑞, 贾海燕, 等. 外源H2S对盐碱胁迫下裸燕麦幼苗生长和生理特性的影响. 麦类作物学报, 2021, 41(2):245-253.
[22] Hu L, Xie Y, Fan S J, et al. Comparative analysis of root transcriptome profiles between drought-tolerant and susceptible wheat genotypes in response to water stress. Plant Science, 2018, 272:276-293.
doi: S0168-9452(17)30854-3 pmid: 29807601
[23] Djanaguiraman M, Prasad P V V, Kumari J, et al. Root length and root lipid composition contribute to drought tolerance of winter and spring wheat. Plant and Soil, 2019, 439(1/2):57-73.
[24] Hassouni K E, Alahmad S, Belkadi B, et al. Root system architecture and its association with yield under different water regimes in durum wheat. Crop Science, 2018, 58(6):2331-2346.
[25] Wei C, Jiao Q J, Agathokleous E, et al. Hormetic effects of zinc on growth and antioxidant defense system of wheat plants. Science of the Total Environment, 2022, 807:150992.
[26] Zhang P Y, Yuan Z, Wei L, et al. Overexpression of ZmPP2C 55 positively enhances tolerance to drought stress in transgenic maize plants. Plant Science, 2022, 314:111127.
[27] Rehman H U, Alharby H F, Bamagoos A A, et al. Sequenced application of glutathione as an antioxidant with an organic biostimulant improves physiological and metabolic adaptation to salinity in wheat. Plant Physiology and Biochemistry, 2021, 158:43-52.
doi: 10.1016/j.plaphy.2020.11.041 pmid: 33296845
[28] Shan C J, Zhang S L, Ou X Q. The roles of H2S and H2O2 in regulating AsA-GSH cycle in the leaves of wheat seedlings under drought stress. Protoplasma, 2018, 255(4):1257-1262.
[29] Madhu, Kaur A, Tyagi S, et al. Exploration of glutathione reductase for abiotic stress response in bread wheat (Triticum aestivum L.). Plant Cell Reports, 2022, 41(3):639-654.
[30] Kaya C, Ugurlar F, Farooq S, et al. Combined application of asparagine and thiourea improves tolerance to lead stress in wheat by modulating AsA-GSH cycle, lead detoxification and nitrogen metabolism. Plant Physiology and Biochemistry, 2022, 190:119-132.
doi: 10.1016/j.plaphy.2022.08.014 pmid: 36113307
[31] Zhao Q, Zhong M, He L, et al. Overexpression of a chrysanthemum transcription factor gene DgNAC1 improves drought tolerance in chrysanthemum. Plant Cell,Tissue and Organ Culture, 2018, 135(1):119-132.
[32] Tounsi S, Jemli S, Feki K, et al. Superoxide dismutase (SOD) family in durum wheat: promising candidates for improving crop resilience. Protoplasma, 2023, 260(1):145-158.
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