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作物杂志,2025, 第2期: 66–73 doi: 10.16035/j.issn.1001-7283.2025.02.009

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

大麦典型硫氧还蛋白（TRX）基因家族鉴定与生物信息学分析

陈虎(), 高原, 孙家猛, 俞鹏, 肖洪武, 张海涛()   

  1. 安徽农业大学农学院，230036，安徽合肥
  • 收稿日期:2024-01-15 修回日期:2024-03-19 出版日期:2025-04-15 发布日期:2025-04-16
  • 通讯作者: 张海涛
  • 作者简介:陈虎，研究方向为作物遗传育种，E-mail：2032242097@qq.com
  • 基金资助:
    “十四五”国家重点研发计划(2022YFF1002903);国家自然科学基金(31871603)

Identification and Bioinformatics Analysis of the Typical Thioredoxin (TRX) Gene Family in Barley

Chen Hu(), Gao Yuan, Sun Jiameng, Yu Peng, Xiao Hongwu, Zhang Haitao()   

  1. College of Agronomy, Anhui Agricultural University, Hefei 230036, Anhui, China
  • Received:2024-01-15 Revised:2024-03-19 Online:2025-04-15 Published:2025-04-16
  • Contact: Zhang Haitao

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摘要：

在栽培大麦MOREX全基因组水平下，利用TBtools、ExPASy、BUSCA、MEME、PlantCARE等在线工具对其染色体定位、理化性质、基因结构、保守结构域和启动子进行了预测分析。利用已发表的RNA-seq数据分析了典型TRX基因家族成员在大麦不同时期、不同组织部位的表达模式及其在干旱胁迫下的表达谱。结果表明，大麦典型TRX基因家族有15个成员，不均匀地分布在6条染色体上（除6H染色体）。启动子顺式作用元件预测到大麦典型TRX基因家族在生长发育、激素调控、非生物胁迫方面均发挥功能。15个典型TRX基因在种子、根、茎、叶和花等不同组织和不同时期均有表达。

关键词: 大麦, TRX基因家族, 系统进化, 表达分析, 生物信息学

Abstract:

The chromosome location, physical and chemical properties, gene structure, conserved domain and promoter were predicted and analyzed by online tools such as TBtools, ExPASy, BUSCA, MEME and PlantCARE at the genome-wide level of cultivated barley MOREX. Using the published RNA-seq data, the expression patterns of typical TRX gene family members in different periods and different tissues of barley and their expression profiles under drought stress were analyzed. The results showed that there were 15 members in the typical TRX gene family of barley, which were unevenly distributed on six chromosomes (except 6H chromosome). Promoter cis-acting elements predicted that the typical TRX gene family of barley played a role in growth and development, hormonal regulation, and abiotic stress. The 15 typical TRX genes were expressed in different tissues such as seeds, roots, stems, leaves and flowers, and at different periods.

Key words: Barley (Hordeum vulgare L.), TRX gene family, System evolution, Expression analysis, Bioinformatics

图1

大麦典型TRX基因在染色体上的位置

表1

大麦典型HvTRX基因的基本信息

基因名称
Gene name		 基因ID
Gene ID		 位置
Position		 氨基酸数
Number of
amino acids		 理论等电点
Theoretical
pI		 分子质量
Molecular
weight		 总平均亲水性
Grand average
hydropathicity		 亚细胞定位
Subcellular
localization
HvTRX1 HORVU.MOREX.r3.1HG0026030 1H 131 5.15 14.48 -0.270 细胞质
HvTRX2 HORVU.MOREX.r3.1HG0070830 1H 143 4.54 15.98 -0.331 细胞核
HvTRX3 HORVU.MOREX.r3.1HG0076510 1H 122 5.12 13.17 0.319 细胞核
HvTRX4 HORVU.MOREX.r3.2HG0149410 2H 118 5.09 12.75 0.089 细胞质
HvTRX5 HORVU.MOREX.r3.2HG0168580 2H 184 8.89 20.26 -0.224 线粒体
HvTRX6 HORVU.MOREX.r3.2HG0183850 2H 240 9.56 25.72 -0.343 叶绿体
HvTRX7 HORVU.MOREX.r3.2HG0194850 2H 577 4.78 63.54 -0.228 细胞质
HvTRX8 HORVU.MOREX.r3.2HG0210290 2H 172 6.74 18.71 -0.058 叶绿体
HvTRX9 HORVU.MOREX.r3.3HG0325100 3H 162 8.40 17.86 -0.200 叶绿体
HvTRX10 HORVU.MOREX.r3.4HG0341820 4H 186 6.32 20.62 -0.308 叶绿体
HvTRX11 HORVU.MOREX.r3.5HG0451950 5H 175 8.52 19.28 0.031 线粒体
HvTRX12 HORVU.MOREX.r3.5HG0479110 5H 319 5.93 35.58 -0.504 细胞核
HvTRX13 HORVU.MOREX.r3.5HG0520730 5H 131 5.89 14.47 -0.065 细胞核
HvTRX14 HORVU.MOREX.r3.7HG0738080 7H 176 9.48 19.35 -0.199 线粒体
HvTRX15 HORVU.MOREX.r3.7HG0749460 7H 173 8.55 18.44 -0.106 叶绿体

图2

大麦典型HvTRX基因家族的系统发育关系（a）、保守基序（b）和基因结构（c） (a) 不同字母代表典型HvTRX的7个亚型。(b) 不同颜色代表10个不同保守基序。(c) 黄色和绿色矩形分别表示编码序列（CDS）和非编码序列（UTR），黑线表示内含子。每个典型HvTRX基因的CDS、UTR和内含子按比例显示长度。

图3

大麦典型HvTRX基因的启动子顺式作用元件分析

图4

使用最大似然法的典型TRX蛋白的系统发育树 、和分别表示大麦、水稻和拟南芥的蛋白质。

图5

大麦、水稻和拟南芥之间典型TRX基因的共线性 灰色线表示大麦和其他作物基因组中的所有共线性块，重复的TRX基因对用黑线突出显示。数字表示上述3种作物基因组的染色体数量。

图6

大麦典型TRX基因在不同组织中的表达模式

图7

干旱胁迫下大麦典型HvTRX基因在叶片（a）和根部（b）的表达热图 CK1和D1：耐旱材料的对照与干旱处理；CK2和D2：干旱敏感材料的对照与干旱处理；CK：对照；WD：缺水处理。

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