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作物杂志,2026, 第5期: 124–140 doi: 10.16035/j.issn.1001-7283.2026.05.015

• • 上一篇    下一篇

苎麻ABC转运蛋白基因家族鉴定及其镉胁迫表达模式分析

顾一舟1(), 颜世伟1, 朱爱国1, 陈坤梅1, 陈佳1, 陈继康1, 高钢1, 王晓飞1, 吴独清1, 杨桢1, 陈平1,2()   

  1. 1 中国农业科学院麻类研究所， 410221， 湖南长沙
    2 岳麓山实验室， 410082， 湖南长沙
  • 收稿日期:2025-05-09 修回日期:2025-07-18 出版日期:2026-10-15 发布日期:2026-10-22
  • 通讯作者: 陈平，主要从事麻类作物遗传育种研究，E-mail：chenping02@caas.cn
  • 作者简介:顾一舟，主要从事苎麻遗传改良及分子育种研究，E-mail：13812981609@163.com
  • 基金资助:
    国家棉麻产业技术体系(CARS-15);中国农业科学院科技创新工程(CAAS-ASTIP-IBFC04);长沙市杰出创新青年培养计划(kq2306017);中国农业科学院南繁专项(YBXM2420)

Identification of the ABC Transporter Protein Gene Family and Expression Pattern Analysis under Cadmium Stress in Ramie (Boehmeria nivea)

Gu Yizhou1(), Yan Shiwei1, Zhu Aiguo1, Chen Kunmei1, Chen Jia1, Chen Jikang1, Gao Gang1, Wang Xiaofei1, Wu Duqing1, Yang Zhen1, Chen Ping1,2()   

  1. 1 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410221, Hunan, China
    2 Yuelushan Laboratory, Changsha 410082, Hunan, China
  • Received:2025-05-09 Revised:2025-07-18 Online:2026-10-15 Published:2026-10-22

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摘要：

挖掘和鉴定重金属修复作物苎麻的ATP结合盒（ATP-binding cassettes，ABC）转运蛋白对重金属污染修复品种的选育与利用具有重要意义。为筛选在镉胁迫中发挥作用的BnABCs，通过生物信息学共鉴定到143个苎麻ABC转运蛋白基因家族成员，其中69%的成员均属于ABCG与ABCB亚家族，大部分位于细胞膜上。与双子叶植物拟南芥和工业大麻分别有57和86对共线性基因，与单子叶植物水稻和玉米分别有31和15对共线性基因。苎麻内发现26对旁系同源基因，其中只有1对Ka/Ks值大于1，表明受到正向选择，其余25对均小于1，表明受到纯化选择。对基因保守结构进行分析，发现BnABCs含有典型的ATP结合结构域组成元件Walker A motifs、Walker B motifs与ABC标签序列（Walker C motifs）。顺式作用元件分析发现，启动子区域有大量光反应、植物激素反应和胁迫反应元件；通过转录组数据筛选出组织特异性表达，且启动子区域含有胁迫元件和MYB结合位点的BnABCs基因，作为响应镉胁迫的候选基因，大部分属于ABCG与ABCB亚家族，且跨膜结构域数目几乎均高于3。实时荧光定量PCR表明其在镉胁迫下的根、茎和叶中呈不同表达模式，其中BnABC128与水稻中镉耐性的基因同源性最高，可能具有外排镉的能力。

关键词: 苎麻, ABC转运蛋白, 基因家族, 镉胁迫, 基因表达模式

Abstract:

The mining and identification of ATP-binding cassette (ABC) transporter proteins in the heavy metal remediation crop ramie are of great significance for the selection and utilization of heavy metal pollution remediation varieties. In order to screen BnABCs that play a role in cadmium (Cd) stress, a total of 143 members of the ABC transporter protein gene family in ramie were identified by bioinformatics, of which 69% belonged to the ABCG and ABCB subfamilies, and most of them were located on cell membrane. 57 and 86 pairs of collinear genes were identified in common with dicotyledonous plants Arabidopsis thaliana and industrial hemp (Cannabis sativa), respectively, as well as 31 and 15 pairs with monocotyledonous plants rice and maize, respectively. 26 pairs of paralogous genes were found in ramie, only one pair of which had Ka/Ks values greater than 1, indicating positive selection, and the remaining 25 pairs had values less than 1, indicating purifying selection. Based on the analysis of gene conserved structures, it was found that BnABCs have typical ATP- binding structural domains consisting of elements Walker A motifs, Walker B motifs and ABC tag sequences (Walker C motifs). Cis-element prediction analysis showed that there were a large number of light-responsive, phytohormone-responsive, and stress-responsive elements in their promoter regions. BnABCs with tissue-specific expression and containing stress-responsive cis-elements and MYB-binding sites in the promoter region were screened from transcriptome data. Most of BnABCs belonged to the subfamilies of ABCG and ABCB, and had transmembrane domains greater than three. The qRT-PCR analysis showed that there were different expression patterns of BnABCs in roots, stems and leaves under cadmium stress, among which, BnABC128 has the highest homology with the gene of cadmium tolerance in rice, indicating the ability of cadmium efflux.

Key words: Ramie, ABC transporter protein, Gene family, Cadmium stress, Gene expression pattern

表1

实时荧光定量PCR引物

基因名称Gene name 正向引物序列（5′-3′）Forward primer sequence (5′-3′) 反向引物序列（5′-3′）Reverse primer sequence (5′-3′)
BnABC3 TCTGTGCCAACAGAGACGAG TGCTCCCTGTGGTTGATGAC
BnABC5 AGCTGTCGTTGGGACGATTT AGACAGCGAGCATCAGTACG
BnABC11 GCGTGATGGACCTCAGCAAGTG CCGACGAGCAGCGAGAAGTAGA
BnABC12 AGGCGTTGAAGTCGTCCATT CATCGATCGCTTGGACAGGA
BnABC13 GCGTCATGGACCTCAGCAAGTG CCGACGAGCAGCGAGAAGTAGA
BnABC38 CAAGTACGCAGCGAGAGCTA GAACCAACGTCACCAGTTGC
BnABC54 GGAGACGTAAGGGATGGAGC TCATAGCAAGCTCGGCCATT
BnABC62 TCAGCGAAAAGGTCCCAGAC CTTGCCACACCAACGTGAAG
BnABC65 TAGCATTAGGTGCCGGGTTG AATCGATCTTCGGGACTCGC
BnABC75 CAAACAGCGTCTACGCATCG CGGGCGTCCCAAGTTAGAAT
BnABC92 ATTGAGATCGGGTCGCACAA GGCCGAGCTGAATTGTCTCT
BnABC98 AGGTCGACTCCAAAGTGAGC ATTTTGCCGAGCGACATCCT
BnABC101 TCCTCAATGCCCATTTGGCT ATGGCTTATTCCACCTCGGC
BnABC118 GGCTCGATAGACACGCGAAT AGAAGTTTCGGCCTCGTCAA
BnABC128 ACTTCTCTTAGGCCCACCCA GCCCGTTGTAGGTGACTCTC
BnABC137 GAAGCAACACCTCGTGGAGA GTTCTCTCCCTAATCCGGCG
18S TGACGGAGAATTAGGGTTC CCGTGTCAGGATTGGGTAATTT

表2

苎麻ABC转运蛋白家族成员理化性质及亚细胞定位预测

基因名称
Gene name		 氨基酸数目
Number of
amino acids		 分子量
Molecular
weight (Da)		 理论等电点
Theoretical pI		 不稳定指数
Instability
index		 脂肪族指数
Aliphatic
index		 总平均亲水范围
Grand average
of hydropathicity		 亚细胞定位
Subcellular
localization
BnABC1 329 35 786.23 5.85 57.57 102.55 -0.005 叶绿体
BnABC10 1067 119 303.23 9.05 43.89 87.47 -0.183 细胞膜
BnABC100 829 93 102.28 9.23 38.57 88.77 -0.115 细胞膜
BnABC101 1471 165 685.83 7.53 39.45 95.03 0.068 细胞膜
BnABC102 1443 163 270.31 7.88 40.00 96.80 0.052 细胞膜
BnABC103 2121 237 315.39 8.52 36.70 89.50 -0.025 细胞膜
BnABC104 954 105 228.38 8.05 37.90 92.65 0.077 细胞膜
BnABC105 698 78 573.22 9.29 36.90 92.11 -0.027 叶绿体
BnABC106 966 108 062.89 8.49 36.90 95.51 0.088 细胞膜
BnABC107 630 68 252.87 7.71 31.98 109.97 0.142 细胞膜
BnABC108 1279 138 484.91 8.31 33.28 101.16 0.153 细胞膜
BnABC109 229 25 909.59 9.62 42.20 108.17 -0.089 叶绿体
BnABC11 761 83 637.71 9.57 47.15 97.56 0.148 细胞膜
BnABC110 266 29 190.96 6.61 28.42 114.29 -0.006 细胞质
BnABC111 1262 137 845.95 9.08 39.08 96.33 0.056 细胞膜
BnABC112 723 79 490.08 9.48 36.79 107.21 0.296 细胞膜
BnABC113 717 81 714.16 8.87 45.76 92.83 0.011 细胞膜
BnABC114 166 17 960.95 9.94 21.57 89.88 -0.040 叶绿体
BnABC115 299 33 831.50 9.58 33.39 96.82 0.419 细胞膜
BnABC116 718 79 999.54 5.72 36.10 82.47 -0.607 细胞质
BnABC117 677 75 129.64 8.52 42.43 89.07 0.020 细胞膜
BnABC118 748 82 933.77 8.81 41.33 91.32 0.054 细胞膜
BnABC119 118 12 434.19 9.24 35.33 72.54 -0.464 细胞膜
BnABC12 707 76 741.94 6.11 34.37 100.01 0.176 细胞膜
BnABC120 507 57 192.68 8.86 42.30 92.64 0.180 细胞膜
BnABC121 150 15 627.12 4.77 46.07 64.87 -0.413 细胞质
BnABC122 724 80 566.04 8.64 42.66 90.43 0.044 细胞膜
BnABC123 605 68 253.71 8.03 32.14 95.65 -0.224 细胞质
BnABC124 456 50 255.76 9.76 51.31 85.13 0.071 细胞膜
BnABC125 173 18 659.63 9.65 24.18 90.75 0.025 叶绿体
BnABC126 1427 160 469.18 8.02 40.79 100.37 0.124 细胞膜
BnABC127 1399 157 363.60 6.89 40.44 99.35 0.136 细胞膜
BnABC128 1437 162 922.61 7.80 40.48 91.60 0.036 细胞膜
BnABC129 700 76 959.98 9.41 40.54 92.23 0.041 细胞膜
BnABC13 764 83 866.95 9.57 47.00 97.43 0.151 细胞膜
BnABC130 700 78 410.17 9.16 45.41 92.87 0.057 细胞膜
BnABC131 1838 205 293.03 7.08 36.32 93.85 0.040 细胞膜
BnABC132 96 10 344.68 9.21 62.96 76.15 -0.417 细胞质
BnABC133 633 71 347.54 9.08 44.09 99.30 0.221 细胞膜
BnABC134 1931 215 756.59 6.80 34.86 95.94 0.090 细胞膜
BnABC135 2192 243 148.60 5.98 42.72 83.95 -0.238 细胞质
BnABC136 639 70 662.25 9.25 37.76 110.23 0.164 细胞膜
BnABC137 1359 153 858.44 6.01 36.70 96.40 0.120 细胞膜
BnABC138 1361 153 484.27 8.47 38.00 93.09 0.084 细胞膜
BnABC139 790 89 381.72 6.74 35.00 96.03 0.196 细胞膜
BnABC14 1107 123 621.15 9.08 43.53 88.63 -0.182 细胞膜
BnABC140 1325 144 689.71 8.17 35.93 94.44 0.034 细胞膜
BnABC141 1063 118 402.84 9.71 56.10 80.15 -0.283 细胞膜
BnABC142 693 75 861.19 8.29 41.79 97.37 0.047 细胞膜
BnABC143 956 107 912.18 8.17 43.32 104.93 0.076 细胞膜
BnABC15 1985 216 409.71 9.22 46.55 85.36 -0.291 细胞膜
BnABC16 654 73 534.47 8.47 39.94 105.63 0.140 细胞膜
BnABC17 1499 169 483.22 8.69 39.44 88.01 0.000 细胞膜
BnABC18 1456 161 252.28 7.68 40.35 109.08 0.235 细胞膜
BnABC19 1374 149 492.74 9.02 42.61 92.08 -0.031 细胞膜
BnABC2 329 35 802.29 5.85 56.98 102.55 0.005 叶绿体
BnABC20 1410 157 000.54 6.22 43.16 105.17 0.221 细胞膜
BnABC21 1427 161 808.11 8.82 42.61 91.87 0.006 细胞膜
BnABC22 1414 159 175.66 6.68 35.91 92.18 0.043 细胞膜
BnABC23 661 71 375.06 8.86 38.29 99.03 0.096 细胞膜
BnABC24 1647 184 320.56 6.16 36.09 102.40 0.070 液泡
BnABC25 1623 182 766.32 7.78 41.35 104.74 0.043 液泡
BnABC26 1504 168 461.41 8.94 39.25 106.43 0.113 液泡
BnABC27 585 65 618.21 6.50 31.46 77.68 -0.394 叶绿体
BnABC28 1249 134 406.97 8.84 40.52 108.29 0.245 细胞膜
BnABC29 346 37 862.32 6.47 42.50 90.38 -0.158 叶绿体
BnABC3 691 77 745.35 5.87 42.27 97.50 -0.122 细胞膜
BnABC30 598 67 041.82 6.11 35.72 90.90 -0.332 叶绿体
BnABC31 687 76 115.42 9.03 45.07 100.17 0.207 细胞膜
BnABC32 1113 122 855.57 9.03 37.63 87.01 -0.105 细胞膜
BnABC33 597 66 855.55 6.03 35.26 91.39 -0.326 细胞质
BnABC34 695 76 990.44 8.94 44.15 100.00 0.204 细胞膜
BnABC35 1171 129 630.69 9.06 36.60 88.11 -0.091 细胞膜
BnABC36 1325 149 128.21 9.31 47.67 98.91 -0.075 细胞膜
BnABC37 702 78 293.93 9.11 40.28 88.63 0.055 细胞膜
BnABC38 678 75 331.68 9.15 44.01 97.05 0.168 细胞膜
BnABC39 1521 171 581.56 8.99 39.39 93.57 0.024 细胞膜
BnABC4 704 78 327.37 8.98 39.53 95.28 0.110 细胞膜
BnABC40 750 82 406.44 9.47 33.27 95.08 -0.015 细胞膜
BnABC41 1365 153 898.34 7.35 42.98 96.29 -0.038 细胞膜
BnABC42 1454 164 236.73 7.17 43.02 98.25 0.016 细胞膜
BnABC43 331 36 795.42 8.29 46.73 93.99 -0.220 细胞质
BnABC44 680 75 770.76 7.96 35.20 95.32 0.167 细胞膜
BnABC45 707 78 368.68 8.53 33.30 94.47 0.130 细胞膜
BnABC46 663 74 173.97 8.97 39.05 98.07 0.108 细胞膜
BnABC47 715 77 839.38 6.23 34.38 100.66 0.189 细胞膜
BnABC48 1411 155 935.63 6.14 48.48 101.51 0.065 细胞膜
BnABC49 663 74 157.97 8.97 39.70 98.07 0.106 细胞膜
BnABC5 715 77 465.97 9.42 35.64 102.81 0.154 细胞膜
BnABC50 1409 158 669.39 8.86 37.04 94.46 0.095 细胞膜
BnABC51 1440 162 701.85 7.06 33.05 90.81 0.034 细胞膜
BnABC52 1399 157 653.09 8.78 35.94 94.44 0.099 细胞膜
BnABC53 1440 162 826.11 7.06 32.81 90.40 0.033 细胞膜
BnABC54 1443 163 131.34 7.27 33.61 90.46 0.018 细胞膜
BnABC55 1526 169 520.35 7.30 36.66 103.86 0.122 细胞膜
BnABC56 1451 160 926.16 7.26 34.36 102.72 0.100 细胞膜
BnABC57 611 68 341.51 9.09 50.23 104.84 0.195 细胞膜
BnABC58 604 64 942.14 8.18 31.59 109.35 0.241 细胞膜
BnABC59 1253 136 569.98 8.43 36.26 99.73 0.122 细胞膜
BnABC6 618 68 677.07 9.00 47.24 116.13 0.378 细胞膜
BnABC60 1416 161 136.58 8.77 39.93 93.06 -0.027 细胞膜
BnABC61 1214 138 282.66 8.14 39.33 96.43 0.037 细胞膜
BnABC62 275 29 914.70 8.97 36.90 96.80 0.304 细胞膜
BnABC63 401 44 158.42 9.41 32.76 106.48 0.143 细胞膜
BnABC64 138 15 331.65 8.84 32.18 89.06 -0.234 细胞膜
BnABC65 1240 133 857.61 7.05 41.09 97.89 0.089 细胞膜
BnABC66 1294 141 492.68 8.52 45.09 94.91 0.006 细胞膜
BnABC67 472 51 962.45 6.44 41.60 95.21 -0.062 细胞膜
BnABC68 658 73 509.27 9.44 43.50 109.53 0.086 细胞膜
BnABC69 160 18 189.44 5.41 43.29 120.62 0.080 细胞膜
BnABC7 399 44 315.80 5.56 43.81 96.04 -0.049 细胞膜
BnABC70 265 29 216.26 9.04 52.27 98.72 0.325 细胞膜
BnABC71 1318 143 829.35 8.67 43.37 93.56 -0.005 细胞膜
BnABC72 1498 167 470.87 6.63 32.82 108.15 0.256 细胞膜
BnABC73 1140 128 564.11 7.57 39.56 105.86 0.117 细胞膜
BnABC74 1485 166 786.94 7.69 40.34 106.67 0.201 细胞膜
BnABC75 1228 134 490.45 8.61 35.05 103.88 0.114 细胞膜
BnABC76 478 51 808.60 6.03 50.00 85.46 -0.260 叶绿体
BnABC77 709 79 001.04 5.70 37.13 89.79 -0.263 叶绿体
BnABC78 1518 168 062.72 8.07 37.84 105.61 0.204 细胞膜
BnABC79 108 12 443.61 5.00 41.89 112.04 -0.203 细胞质
BnABC8 1473 164 206.31 8.23 36.14 103.61 0.136 细胞膜
BnABC80 284 31 495.17 6.08 47.56 91.62 -0.132 细胞质
BnABC81 1505 168 501.65 8.39 37.23 103.36 0.163 细胞膜
BnABC82 675 74 654.83 7.98 37.69 92.92 0.036 细胞膜
BnABC83 150 16 509.76 4.87 42.84 94.93 -0.099 细胞质
BnABC84 731 79 316.15 8.76 39.43 92.38 -0.029 细胞膜
BnABC85 264 29 407.77 5.34 37.81 93.11 -0.035 细胞膜
BnABC86 605 68 280.74 7.80 36.37 95.34 -0.230 细胞质
BnABC87 304 34 736.06 9.20 37.45 107.11 0.373 高尔基体
BnABC88 716 78 153.08 9.07 36.99 93.80 0.087 细胞膜
BnABC89 461 51 398.71 8.97 39.00 105.75 0.258 细胞膜
BnABC9 1263 139 196.94 8.78 43.68 101.41 0.045 细胞膜
BnABC90 1252 137 490.25 9.36 37.03 97.84 0.042 细胞膜
BnABC91 1426 159 285.37 8.40 35.73 104.87 0.102 细胞膜
BnABC92 1208 131 757.85 9.15 38.42 96.67 0.061 细胞膜
BnABC93 1422 161 910.16 8.18 39.01 93.65 0.067 细胞膜
BnABC94 602 67 293.34 8.97 42.36 103.90 0.228 细胞膜
BnABC95 1422 161 910.20 8.30 38.77 93.52 0.065 细胞膜
BnABC96 1281 137 921.08 8.71 34.63 99.32 0.135 细胞膜
BnABC97 1281 138 054.31 8.65 35.15 100.01 0.146 细胞膜
BnABC98 723 80 728.35 6.40 39.43 86.13 -0.451 细胞质
BnABC99 1248 135 387.58 8.60 33.95 107.35 0.208 细胞膜

图1

苎麻ABC基因家族成员染色体定位 染色体上的颜色代表基因密度。

图2

物种间共线性分析 (a) 苎麻与双子叶植物拟南芥和工业大麻间的共线性分析；(b) 苎麻与单子叶植物水稻和玉米间的共线性分析。

图3

苎麻物种内的共线性分析 染色体上的颜色代表基因密度，黑色线条代表GC含量。

表3

苎麻物种内同源基因对的Ka/Ks计算

序号
Number		 基因名称1
Gene name 1		 基因名称2
Gene name 2		 基因复制类型
Types of gene replication		 Ka Ks Ka/Ks 选择类型
Selective type
1 BnABC10 BnABC14 全基因组或基因片段复制 0.0029 0.0311 0.0920 纯化选择
2 BnABC13 BnABC141 全基因组或基因片段复制 0.1916 1.0169 0.1885 纯化选择
3 BnABC10 BnABC35 全基因组或基因片段复制 0.2404 2.0903 0.1150 纯化选择
4 BnABC12 BnABC47 全基因组或基因片段复制 0.0101 0.0303 0.3348 纯化选择
5 BnABC7 BnABC72 全基因组或基因片段复制 0.1770 3.4031 0.0520 纯化选择
6 BnABC107 BnABC58 全基因组或基因片段复制 0.1285 1.8864 0.0681 纯化选择
7 BnABC114 BnABC125 全基因组或基因片段复制 0.0160 0.0432 0.3707 纯化选择
8 BnABC115 BnABC124 全基因组或基因片段复制 0.0000 0.0135 0.0000 纯化选择
9 BnABC117 BnABC119 全基因组或基因片段复制 0.2610 0.4263 0.6123 纯化选择
10 BnABC126 BnABC21 全基因组或基因片段复制 0.2313 2.3434 0.0987 纯化选择
11 BnABC130 BnABC132 全基因组或基因片段复制 0.0423 0.0287 1.4705 正向选择
12 BnABC131 BnABC134 全基因组或基因片段复制 0.0014 0.0095 0.1495 纯化选择
13 BnABC137 BnABC52 全基因组或基因片段复制 0.2119 2.0754 0.1021 纯化选择
14 BnABC137 BnABC50 全基因组或基因片段复制 0.2114 2.1753 0.0972 纯化选择
15 BnABC30 BnABC33 全基因组或基因片段复制 0.0051 0.0222 0.2298 纯化选择
16 BnABC31 BnABC34 全基因组或基因片段复制 0.0026 0.0265 0.0971 纯化选择
17 BnABC32 BnABC35 全基因组或基因片段复制 0.0008 0.0132 0.0601 纯化选择
18 BnABC50 BnABC52 全基因组或基因片段复制 0.0028 0.0369 0.0770 纯化选择
19 BnABC44 BnABC45 全基因组或基因片段复制 0.0272 0.0660 0.4118 纯化选择
20 BnABC46 BnABC49 全基因组或基因片段复制 0.0027 0.0320 0.0828 纯化选择
21 BnABC63 BnABC70 全基因组或基因片段复制 0.2962 1.9769 0.1498 纯化选择
22 BnABC57 BnABC94 全基因组或基因片段复制 0.2845 2.7581 0.1032 纯化选择
23 BnABC87 BnABC88 全基因组或基因片段复制 0.0043 0.0325 0.1340 纯化选择
24 BnABC84 BnABC85 全基因组或基因片段复制 0.0039 0.0138 0.2830 纯化选择
25 BnABC93 BnABC95 全基因组或基因片段复制 0.0009 0.0119 0.0777 纯化选择
26 BnABC96 BnABC97 全基因组或基因片段复制 0.0031 0.0185 0.1673 纯化选择

图4

苎麻与拟南芥ABC基因家族成员系统进化树分析 黑色圆代表苎麻ABC转运蛋白，白色三角代表拟南芥ABC转运蛋白。

图5

苎麻ABC基因家族成员蛋白保守基序及标志图、保守结构域、基因结构分析 (a)和(d)分别为蛋白质保守基序结构图与标志图，(b)为保守结构域结构图，(c)为基因结构图。

图6

苎麻ABC基因家族成员启动子顺式作用元件预测 红色方框中为不同组织特异性表达基因。

图7

苎麻ABC基因家族成员不同苎麻部位中表达量

表4

候选基因蛋白二级结构与跨膜结构域数目

基因名称
Gene name		 α-螺旋比例
Alpha helix
ratio (%)		 β-转角比例
Beta turn
ratio (%)		 无规卷曲比例
Random coil
ratio (%)		 延伸链比例
Extended strand
ratio (%)		 跨膜结构域数目
Number of
transmembrane domains		 亚家族名称
Subfamily
name
BnABC3 52.10 3.04 34.15 10.71 5 ABCD
BnABC5 53.01 4.20 31.05 11.75 3 ABCB
BnABC11 41.66 3.81 43.76 10.78 6 ABCG
BnABC12 44.84 4.81 38.90 11.46 5 ABCG
BnABC13 43.85 3.53 42.80 9.82 6 ABCG
BnABC38 48.53 4.28 35.25 11.95 7 ABCG
BnABC54 51.77 2.91 35.76 9.56 13 ABCG
BnABC62 66.91 4.36 17.09 11.64 3 ABCB
BnABC65 56.85 4.52 25.16 13.47 8 ABCB
BnABC75 56.03 4.89 25.00 14.09 10 ABCB
BnABC92 55.88 3.64 27.32 13.16 9 ABCB
BnABC98 45.92 5.12 35.55 13.42 0 ABCF
BnABC101 51.19 2.79 35.69 10.33 13 ABCG
BnABC118 43.45 3.48 39.44 13.64 6 ABCG
BnABC128 50.73 3.34 35.56 10.37 13 ABCG
BnABC137 51.36 2.87 35.17 10.60 13 ABCG

图8

镉胁迫下苎麻ABC转运蛋白候选基因的组织表达模式 不同小写字母表示在P < 0.05水平差异显著。

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