Crops ›› 2016, Vol. 32 ›› Issue (2): 133-138.doi: 10.16035/j.issn.1001-7283.2016.02.025

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Effects of Epi-Brassinosteroid on Panicle Traits of Rice during Panicle Differentiation Stage

Gong Yanlong,Xu Hai,Xia Yuanye,Du Zhimin,Shi Xiaorui,Xu Zhengjin   

  1. Rice Institute,Shenyang Agricultural University/Key Laboratory of Northeast Rice Biology and Breeding,Ministry of Agriculture/Key Laboratory of Northern Japonica Super Rice Breeding,Ministry of Education/Key Laboratory of Northern Japonica Genetics and Breeding of Liaoning Province,Shenyang 110866,Liaoning,China
  • Received:2015-11-23 Revised:2016-02-28 Online:2016-04-15 Published:2018-08-26
  • Contact: Zhengjin Xu

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Abstract:

Five rice cultivars were used as test materials to study the effects that spraying epi-brassinosteroid with lower, medium and high concentrations on the panicle traits of rice during panicle differentiation stages, which including bract differentiation stage(T1), branch differentiation stage(T2), spikelet primordium differentiation stage(T3), pollen mother cell formation and meiosis stage(T4), pollen filling stage(T5). The results were as follows, panicle length and grain number were significantly higher than that of the control. The difference on seed setting rate of primary branch was not significant, but epi-BR with medium concentrations could significantly increase seed setting rate of secondary branch, so promote the seed setting rate of whole panicle. Grains weight (include thousand grains weight of primary and secondary branch) fell significantly after applying epi-BR. Larger the concentrations, lower the seed setting rate. Grain density fell significantly after applying epi-BR, but low and medium concentrations of epi-BR were more effective on decreasing grains density than that of high concentrations. After spraying epi-BR at different periods of panicle differentiation, the effect of promoting panicle length was T4>T1>T5>T3>T2, the effect of promoting grain number was T1>T3>T2>T4>T5. The effect of promoting panicle seed setting rate was more obvious at the pollen mother cell formation and meiosis stage(T4), the effects of different spraying periods on the differences of branch number and thousand grains weight were not significant, the more delay the spraying periods, the more obvious the effect of grains density decreased. There was interaction effect between the spraying periods and concentrations. We made sure the best combination of spraying periods and concentrations aimed at regulating effect of different panicle traits.

Key words: Rice, Epi-brassinosteroid, Panicle traits, Panicle differentiation stage

Fig.1

Effect of epi-BR with different concentrations on panicle length Different small letters mean 0.05 level significant differences among treatments. The same below."

Fig.2

Effect of epi-BR with different concentrations on number of branch"

Fig.3

Effect of epi-BR with different concentrations on grains per panicle"

Fig.4

Effect of epi-BR with different concentrations on seed setting rate"

Fig.5

Effect of epi-BR with different concentrations on thousand grains weight"

Fig.6

Effect of epi-BR with different concentrations on grains density"

Fig.7

Effect of epi-BR treated at different stage on panicle length"

Fig.8

Effect of epi-BR treated at different stage on number of branch"

Fig.9

Effect of epi-BR treated at different stage on grains per panicle"

Fig.10

Effect of epi-BR treated at different stage on seed setting rate"

Fig.11

Effect of epi-BR treated at different stage on thousand grains weight"

Fig.12

Effect of epi-BR treated at different stage on grains density"

Table 1

Optimal spraying stages and concentrations of epi-BR for regulating panicle traits"

性状
Trait		 调控方向
Regulatory direction		 施用时期和浓度最佳组合
Optimal combination of spraying stage and concentration
穗长Panicle length 促进Increase T4C3
一次枝梗数Number of primary branch 促进Increase T1C2
二次枝梗数Number of secondary branch 促进Increase T1C2
穗粒数Grains per panicle 促进Increase T1C2
一次枝梗结实率Seed setting rate of primary branch 促进Increase T5C2
二次枝梗结实率Seed setting rate of secondary branch 促进Increase T4C2
整穗结实率Seed setting rate of whole panicle 促进Increase T4C2
一次枝梗千粒重Thousand grains weight of primaty branch 抑制Decrease T3C3
二次枝梗千粒重Thousand grains weight of secondary branch 抑制Decrease T4C1
总千粒重Thousand grains weight 抑制Decrease T3C3
着粒密度Grains density 抑制Decrease T5C2
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