Crops ›› 2017, Vol. 33 ›› Issue (1): 144-149.doi: 10.16035/j.issn.1001-7283.2017.01.026

Previous Articles     Next Articles

The Influence of Resistant and Susceptible Soybean Germplasm on Growth Dynamic Changes of Soybean Cyst Nematode Race 3（SCN3）

Li Haiyan1,Cai Deli1,Chen Jingsheng2,Duan Yuxi3,Chen Lijie3,Shang Yingyu1   

  1. 1College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163316,Heilongjiang,China
    2Daqing Branch of Heilongjiang Academy of Sciences,Daqing 163316,Heilongjiang,China
    3Nematology Institute of Northern China,College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,Liaoning,China
  • Received:2016-09-27 Revised:2016-11-04 Online:2017-02-15 Published:2018-08-26

RichHTML

4

PDF (PC)

116

Abstract:

A pot experiment was conducted with resistant cultivars Wuzhai Heidou, 07-2-10-6 and susceptible cultivars Hefeng 35, 07-2-7-14. The influence of different germplasm to growth dynamic changes of soybean cyst nematode race 3（SCN3）were determined. The results indicated that root exudates had an important effect on the egg hatch of SCN3. Root exudates of Hefeng 35 and 07-2-7-14 stimulated egg incubation of SCN3, while root exudates of Wuzhai Heidou and 07-2-10-6 inhibited egg incubation of SCN 3. After incubating 10 days, the J2 hatching rate of Hefeng 35, 07-2-7-14 were 3.71 and 4.38, respectively, while the J2 hatching rate of Wuzhai Heidou, 07-2-10-6 were 0.89 and 1.39, respectively. The dynamic changes of SCN3 in soybean root indicated that the number of J2 seldom invaded into resistant cultivars. There was only a part of larva at J2 stage that could develop to J3 stage, and their growth was delayed that the ratio of female to male was 1:4.13. But there was a large number of J2 stage larva that invaded into susceptible cultivars, and most of them could develop to J3 stage. The growth speed of larva in susceptible cultivars was faster than that in resistant cultivars, and the ratio of female to male was 10.56:1. The resistant mechanism of Wuzhai Heidou to SCN 3 was integrated effects of anti-intrusion, anti-growth and anti-reproduction.

Key words: Resistant and susceptible soybean germplasm, Soybean cyst nematode, Race 3 (SCN3), Root exudates, Growth dynamic changes

Fig.1

The difference number of SCN J2 on root to hybrids and their parents The different lowercase letters represented significant differences,the same below"

Fig.2

The infuluences of hybrids and their parents root-exudates on the hatching of cyst"

Fig.3

J2 numbers of hybrids and their parents root-exudates on the hatching of cyst during 10 days"

Fig.4

The J2 hatching rate of hybrids and their parents root-exudates during 10 days"

Table 1

The J2 numbers of different age seedling root-exudates on the hatching of cyst during 10 days"

品种（系）
Varieties (lines)		 苗龄Age seedling
6d 18d 30d
五寨黑豆Wuzhai Heidou 169d 174b 134c
07-2-10-6 262c 303b 313b
合丰35 Hefeng 35 697b 1 221a 1 364a
07-2-7-14 824a 1 033a 1 432a

Table 2

The number of J2 SCN in hybrids and their parents 5 days after inoculation"

品种（系）Varieties(lines) I II III 平均值The average value
合丰35 Hefeng35 42 66 49 52.3±7.13aA
07-2-7-14 38 58 42 46.0±6.11aAB
07-2-10-6 15 32 16 21.0±5.51bAB
五寨黑豆Wuzhai Heidou 15 16 13 14.7±0.88bB

Table 3

The number of SCN race 3 at each stage in the soybean roots after inoculation different days"

品种（系）
Varieties(lines)		 6d 12d 18d 24d 30d
J2 J3 J4 J2 J3 J4 J2 J3 J4 J2 J3 J4 J2 J3 J4
五寨黑豆Wuzhai Heidou 14.7c 0.0c 0.0 39.6d 5.7d 0.0c 29.7b 14.3c 6.9d 10.4a 0.0c 17.1bc 7.3a 0.0 3.3a
合丰35 Hefeng 35 80.7b 13.4a 1.3 161.3b 31.3b 10.7ab 33.7ab 39.7b 56.3c 0.0c 3.3b 22.1b 5.3b 0.0 0.0b
07-2-10-6 53.7b 0.0c 0.0 80.6c 19.7c 6.7b 41.3a 35.3b 17.7b 3.6b 8.7a 10.0c 0.0c 0.0 3.7a
07-2-7-14 111.4a 6.1b 0.0 201.5a 47.7a 13.2a 25.5b 67.8a 73.9a 0.0c 0.0c 33.7a 0.0c 0.0 0.0b

Fig.5

The effect on female and male ratio of the SCN in the root of different soybean cultivars"

[1] 李国桢, 杨兆英, 王守义 , 等. 抗大豆孢囊线虫病育种的进展.大豆通报, 1993(3):27-29.
[2] 刘维志 . 植物病原线虫学.北京: 中国农业出版社, 2000.
[3] 韩雪, 吴凤芝, 潘凯 . 根系分泌物与土传病害关系之研究综述. 植物保护科学, 2006,22(2):316-318.
[4] 张福锁, 陈青, 曹一平 , 等. 根系分泌物及其对根际微生物生态系统中养分有效性的直接影响.土壤与植物营养研究新动态.北京: 北京农业大学出版社, 1992: 64-72.
[5] Graham T L . Flavonoid and isoflavonoid distribution in developing soybean seeding issues and in seed and root exudates. Plant Physiology, 1991,95:594-603.
doi: 10.1104/pp.95.2.594
[6] 许艳丽, 陈伊里, 司兆胜 , 等. 不同茬口条件下的作物根渗出物对大豆Heterodera glycines卵孵化影响. 植物病理学报, 2004,34(6):481-486.
[7] 袁虹霞, 李洪连, 王烨 , 等. 棉花不同抗性材料根系分泌物分析及其对黄萎病菌的影响. 植物病理学报, 2002,32(2):127-131.
[8] 陈捷胤, 戴小枫 . 棉花对黄萎病的抗病机制研究进展. 分子植物育种, 2005,3(3):427-435.
[9] Tefft P M . Factors influencing eggs hatching of the soybean cyst nematode Heterodera glycines race 3. Proceedings of the Helminthological Society of Washington, 1982,49:258-265.
[10] Tefft P M, Bone L W . Plant-induced hatching of eggs of soybean cyst nematode Heterodera glycines. Journal of Nematology, 1985,17(3):275-279.
[11] 杨岱伦 . 大豆孢囊线虫的生物学研究.辽宁农业科学, 1984(5):23-26.
[12] 刘维志, 刘烨, 段玉玺 , 等. 抗病品种对大豆孢囊线虫的选择作用. 植物保护学报, 1993,20(2):135-137.
[13] 刘晔, 刘维志, 段玉玺 , 等. 大豆孢囊线虫在不同大豆品种根内的发育.辽宁农业科学, 1988(4):16-18.
[14] 王雪 . 大豆抗胞囊线虫机制及与抗性相关的差异蛋白质组学研究. 沈阳:沈阳农业大学, 2008.
[15] 颜清上, 陈品三, 王连铮 . 大豆根渗出物对大豆胞囊线虫4号生理小种卵孵化的影响. 植物病理学报, 1997,27(3):269-274.
[16] Schmitt D P, Riggs R D . Influence of selected plant species on hatching of eggs and development of juveniles of Heterodera glycines. Journal of Nematology, 1991,23(1):1-6.
[17] 张海平, 王志, 吴书峰 . 大豆胞囊线虫 4 号生理小种不同抗性材料根系分泌物对其抗病性的影响. 大豆科学, 2016,35(2):285-290.
[18] Anand S C, Sharma S B . Genetic relationships for resistance to Heterodera glycines race 3 and in soybean. Journal of Nematology, 1996,28(2):223-237.
[19] 刘维志 . 植物线虫学研究技术.沈阳: 辽宁科学技术出版社, 1995.
[20] 吴海燕 . 大豆与大豆胞囊线虫相互关系研究. 沈阳:沈阳农业大学, 2003.
[21] 颜清上, 陈品三, 王连铮 , 等. 中国小黑豆抗源对大豆胞囊线虫4号生理小种抗性机制的研究,Ⅰ抗源品种对大豆胞囊线虫侵染和发育的影响. 植物病理学报, 1996,26(4):317-323.
[22] 刘大伟 . 灰皮支黑豆对大豆胞囊线虫3号生理小种抗性机制研究. 沈阳:沈阳农业大学, 2011.
[23] 董宇, 段玉玺, 陈立杰 , 等. 应县小黑豆与大豆胞囊线虫3号生理小种的互作关系. 中国农学通报, 2009,25(6):195-199.
[1] Peina Lu,Jinghui Liu,Baoping Zhao,Lijun Li,Xuefeng Lei,Fengyi Zhang. Effects of Microbial Fertilizer on Soil Characteristics and Root Exudates of Oats in Saline-alkali Land [J]. Crops, 2017, 33(5): 85-92.
[2] Guoyong Ren,Wei Li,Lifeng Zhang,Caijie Wang,Haiying Dai,Jinlong Wang,Ran Xu,Yanwei Zhang. The Resistance to Soybean Cyst Nematode Race 1 of Transgenic Soybean with hrf2 Encoding HarpinXooc [J]. Crops, 2017, 33(3): 49-53.
[3] Haiping Zhang,Shufeng Wu,Zhi Wang. Inheritance of Resistance to Race 4 of Cyst Nematode in Soybean [J]. Crops, 2016, 32(3): 21-26.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production[J]. Crops, 2018, 34(4): 1 -7 .
[2] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut[J]. Crops, 2018, 34(4): 102 -105 .
[3] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[4] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[5] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System[J]. Crops, 2018, 34(4): 114 -120 .
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation[J]. Crops, 2018, 34(4): 121 -125 .
[7] Xiaofei Wang,Haijun Xu,Mengqiao Guo,Yu Xiao,Xinyu Cheng,Shuxia Liu,Xiangjun Guan,Yaokun Wu,Weihua Zhao,Guojiang Wei. Effects of Sowing Date, Density and Fertilizer Utilization Rate on the Yield of Oilseed Perilla frutescens in Cold Area[J]. Crops, 2018, 34(4): 126 -130 .
[8] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[9] Jie Gao,Qingfeng Li,Qiu Peng,Xiaoyan Jiao,Jinsong Wang. Effects of Different Nutrient Combinations on Plant Production and Nitrogen, Phosphorus and Potassium Utilization Characteristics in Waxy Sorghum[J]. Crops, 2018, 34(4): 138 -142 .
[10] Na Shang,Zhongxu Yang,Qiuzhi Li,Huihui Yin,Shihong Wang,Haitao Li,Tong Li,Han Zhang. Response of Cotton with Vegetative Branches to Plant Density in the Western of Shandong Province[J]. Crops, 2018, 34(4): 143 -148 .