Crops ›› 2017, Vol. 33 ›› Issue (3): 49-53.doi: 10.16035/j.issn.1001-7283.2017.03.010

Previous Articles     Next Articles

The Resistance to Soybean Cyst Nematode Race 1 of Transgenic Soybean with hrf2 Encoding HarpinXooc

Ren Guoyong,Li Wei,Zhang Lifeng,Wang Caijie,Dai Haiying,Wang Jinlong,Xu Ran,Zhang Yanwei   

  1. Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250131,Shandong,China
  • Received:2017-02-22 Revised:2017-04-06 Online:2017-06-15 Published:2018-08-26
  • Contact: Yanwei Zhang

RichHTML

4

PDF (PC)

122

Abstract:

The Harpin protein could make plants produce broad-spectrum resistance to stress by activating the defense pathways of plants. HarpinXooc protein encoded by hrf2 gene has similar function to Harpin protein. In this study, the evaluation was performed to estimate the resistance to soybean cyst nematode (SCN) of transgenic soybean with hrf2 gene. The result showed that the SCN number of two lines of transgenic soybean with hrf2 gene was significantly lower than that of the receptor plant Tianlong 1, indicating that hrf2 gene might improve soybean resistance to SCN race 1.

Key words: Harpin, hrf2, Soybean, Soybean cyst nematode, Race 1

Table 1

Primer sequence"

引物名称Primer name 上游引物序列Forward primer sequence (5'-3') 下游引物序列Reverse primer sequence (5'-3') PCR产物PCR product (bp)
hrf2 CGGGATCCATGAACTCTTTGAACACACA CGAGCTCTTACTGCATTGATGCGCT 414
Bar CAGCTGCCAGAAACCCACGT CTGCACCATCGTCAACCACT 436

Table 2

The evaluation criterion of soybean resistance to SCN"

抗性评价Resistance evaluation 免疫Immunity 高抗Resistant 中抗Moderately resistant 感Susceptible 高感High susceptible
单株着生雌虫平均数
Average of cyst nematode per plant		 0
 0.1~3.0
 3.1~10.0
 10.1~30.0
 30.0以上

Fig.1

The PCR detection for hrf2 of transgenic soybean"

Fig.2

The PCR detection for Bar of transgenic soybean Positive: pCAMBIA3300::hrf2; M: D2000 of Tiangen; CK: Tianlong 1; A1-A6: Six plants of T2-24-1; B1-B5: Five plants of T2-5-1"

Fig.3

The detection of transgenic soybean by Bar strip CK: Tianlong 1; A1, A2, A4-A7: Six plants of T2-24-1; B2, B3, B6: Three plants of T2-5-1"

Fig.4

The resistance to PPT of transgenic soybean CK: Tianlong 1; A1, A2: Two plants of T2-24-1; B2, B3: Two plants of T2-5-1"

Table 3

The resistance to SCN of soybean plants"

株系Lines 观测数No. of plants 最大值Max 最小值Min 单株胞囊数No. of cytocyst per plant 抗性级别The resistance level
Peking 8 7 0 2.13±1.02 R
Lee 8 81 47 61.88±5.26 HS
Pickett 7 5 0 3.57±0.52 MR
PI88788 8 45 29 37.13±4.26 HS
PI90763 7 3 0 1.29±0.32 R
天隆一号Tianlong 1 5 47 35 43.00±2.10 HS
T2-24-1 32 47 5 21.66±13.10 S
T2-5-1 9 22 4 15.22±6.69 S

Fig.5

The resistance to SCN of transgenic soybean CK: Tianlong 1; **: Highly significant difference (t test, P≤0.01)"

[1] Wei Z M, Laby R J, Zumoff C H , et al. Harpin,elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora. Science, 1992,257(5066):85-88.
doi: 10.1126/science.1621099
[2] Dong H, Delaney T P, Bauer D W , et al. Harpin induces disease resistance in Arabidopsis through the systemic acquired resistance pathway mediated by salicylic acid and the NIM1 gene. Plant Journal, 1999,20(2):207-215.
doi: 10.1046/j.1365-313x.1999.00595.x
[3] Dong H P, Yu H, Bao Z , et al. The ABI2-dependent abscisic acid signalling controls HrpN-induced drought tolerance in Arabidopsis. Planta, 2005,221(3):313-327.
doi: 10.1007/s00425-004-1444-x
[4] 姚桢 . Harpin促进植物生长和诱导抗旱的信号传导解析以及作用定位的初步研究. 南京:南京农业大学, 2004.
[5] 汤承 . HarpinEcc蛋白对园艺植物的生物学效应及其诱导抗性的分子机制解析. 重庆:西南大学, 2006.
doi: 10.7666/d.y937843
[6] 董宏平, 李明立, 彭建令 , 等. HarpinEa促进烟草和蕃茄生长和诱导抗虫的信号通路. 高技术通讯, 2004,14(12):29-32.
doi: 10.3321/j.issn:1002-0470.2004.12.007
[7] 李梅, 毕阳, 葛永红 , 等. 采前Harpin处理对“银帝”甜瓜潜伏侵染的影响. 甘肃农业大学学报, 2005,40(2):153-156.
doi: 10.3969/j.issn.1003-4315.2005.02.006
[8] 唐利, 朱春花, 李静 , 等. Harpin蛋白处理对苹果采后腐烂及品质的影响. 安徽农学通报, 2011,17(22):20.
doi: 10.3969/j.issn.1007-7731.2011.22.013
[9] 张拥华, 李世东, 刘杏忠 . 葡聚烯糖和Harpin蛋白对两种番茄病害的防治效果.中国生物防治, 2003(2):95-96.
[10] 邵敏, 陈功友, 王金生 . 水稻白叶枯病菌蛋白质激发子HarpinXoo诱导植物的防卫反应.农业生物技术学报, 2003(2):192-197.
doi: 10.3969/j.issn.1674-7968.2003.02.017
[11] 李平, 陆徐忠, 邵敏 , 等. 水稻黄单胞细菌Harpin蛋白的遗传多样性及其诱导烟草过敏反应和抗病性功能. 中国科学:生命科学, 2004,34(2):136-143.
[12] Peng J L, Bao Z L, Ren H Y , et al. Expression of harpinXoo in transgenic tobacco induces pathogen defense in the absence of hypersensitive cell death. Phytopathology, 2004,94(10):1048-1055.
doi: 10.1094/PHYTO.2004.94.10.1048
[13] 邵敏, 王金生 . 转hrfAXoo基因水稻对白叶枯病的抗性. 南京农业大学学报, 2004,27(4):36-40.
[14] Zhang L, Xiao S, Li W , et al. Overexpression of a Harpin-encoding gene hrf1 in rice enhances drought tolerance. Journal of Experimental Botany, 2011,62(12):4229-4238.
doi: 10.1093/jxb/err131 pmid: 3153678
[15] Li W, Shao M, Zhong W , et al. Ectopic expression of Hrf1 enhances bacterial resistance via regulation of diterpene phytoalexins,silicon and reactive oxygen species burst in rice. Plos One, 2012,7(9):e43914.
doi: 10.1371/journal.pone.0043914
[16] Huo R, Wang Y, Ma L L , et al. Assessment of inheritance pattern and agronomic performance of transgenic rapeseed having harpinXooc-encoding hrf2 gene. Transgenic Research, 2010,19(5):841-847.
doi: 10.1007/s11248-010-9365-x
[17] 马玲莉, 霍蓉, 高学文 , 等. 转harpinXooc蛋白编码基因hrf2对油菜抗菌核病的影响. 中国农业科学, 2008,41(6):1655-1660.
[18] 魏桂芳 . 大豆转HarpinXooc蛋白编码基因(hrf2)及其在后代中的遗传表达研究. 南京:南京农业大学, 2014.
[19] Riggs R D, Schmitt D P . Complete characterization of the race scheme for Heterodera glycines. Journal of Nematology, 1988,20(20):392-395.
doi: 10.1016/0022-2011(88)90126-7 pmid: 19290228
[20] 刘大伟 . 灰皮支黑豆对大豆胞囊线虫3号生理小种抗性机制研究. 沈阳:沈阳农业大学, 2011.
[21] 李海燕 . 五寨黑豆对大豆胞囊线虫3号生理小种抗性机制研究. 沈阳:沈阳农业大学, 2015.
[1] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System [J]. Crops, 2018, 34(4): 114-120.
[2] Mingjun Zhang,Zhongfeng Li,Lili Yu,Jun Wang,Lijuan Qiu. Identification and Screening of Protein Subunit Variation Germplasm from Both Mutants and Natural Population in Soybean [J]. Crops, 2018, 34(3): 44-50.
[3] Jiani Zhu,Huiping Dai,Shuhe Wei,Genliang Jia,Dejing Chen,Jinjin Pei,Qing Zhang,Long Qiang. Effects of Applying Zn on the Growth and Zn Accumulation in Soybean at Flowering Stage [J]. Crops, 2018, 34(1): 152-155.
[4] Tianle Ma,Jianxin Zhang. Effects of Different Multiple Cropping Methods on Dry Matter Accumulation, Distribution and Yield of Summer Soybean [J]. Crops, 2018, 34(1): 156-159.
[5] Lina Li,Longguo Jin,Chuanxiao Xie,Changlin Liu. Determining Blind Samples of Transgenic Maize and Transgenic Soybean [J]. Crops, 2017, 33(6): 37-44.
[6] Xuechao Zhou,Surong Ding,Yunshan Wei,Yanfang Zhou,Xue Wei,Rina Na,Feng Li. Evaluation of Adaptability of Different Vegetable Soybean Cultivars (Lines) in Chifeng Area [J]. Crops, 2017, 33(3): 44-48.
[7] Xuli Zhang,Baolong Xing,Guimei Wang,Lili Yin. Effects of Planting Density on Agronomic Traits, Economic Traits and Yield of Soybean in North of Shanxi Province [J]. Crops, 2017, 33(3): 127-131.
[8] Xiting Zhang,Liwei Cao,Shucai Lü,Guoxing Chen,Yongji Wang,Shuhan Yu,Zhenping Gong. Effects of Bulk Density on Nitrogen Absorption and Yield of Soybean on Black Soil [J]. Crops, 2017, 33(3): 132-137.
[9] Yixin Tian,Fengju Gao. The Response of Growth and Dry Matter Accumulation and Distribution of High Protein Soybean to Plant Density [J]. Crops, 2017, 33(2): 121-125.
[10] Li Yan,Qiang Yang,Yupeng Shao,Dandan Li,Zhikun Wang,Wenbin Li. Cloning and Sequence Analysis of GmWRI1a Gene Promoter in Soybean [J]. Crops, 2017, 33(2): 51-58.
[11] Yu Gao,Yanchao Liu,Shusen Shi,Juan Cui,Jinfeng Xiong. Review on Soybean Thrips in China [J]. Crops, 2017, 33(1): 8-13.
[12] Haiyan Li,Deli Cai,Jingsheng Chen,Yuxi Duan,Lijie Chen,Yingyu Shang. The Influence of Resistant and Susceptible Soybean Germplasm on Growth Dynamic Changes of Soybean Cyst Nematode Race 3（SCN3） [J]. Crops, 2017, 33(1): 144-149.
[13] Qianxu Zhao,Xianrong Yue,Yunsheng Xia,Naiming Zhang,Fuzhao Nian,Yunqiang Yang,Yulin Ma. Effects of Arbuscular Mycorrhizal Fungus Inoculation on Growth and Nitrogen Utilization of Intercropped Maize and Soybean in Purple Soil under Facilitated Condition [J]. Crops, 2016, 32(5): 94-100.
[14] Zhao Geng,Youbin Kong,Lili Zhao,Cui Liu,Hui Du,Xihuan Li,Caiying Zhang. Transformation of GmPHR1 and GmPAP4 Related to High Phosphorus Efficiency and Elite Germplasm Enhancement in Soybean (Glycine max) [J]. Crops, 2016, 32(3): 58-62.
[15] Haiping Zhang,Shufeng Wu,Zhi Wang. Inheritance of Resistance to Race 4 of Cyst Nematode in Soybean [J]. Crops, 2016, 32(3): 21-26.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production[J]. Crops, 2018, 34(4): 1 -7 .
[2] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut[J]. Crops, 2018, 34(4): 102 -105 .
[3] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[4] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[5] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System[J]. Crops, 2018, 34(4): 114 -120 .
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation[J]. Crops, 2018, 34(4): 121 -125 .
[7] Xiaofei Wang,Haijun Xu,Mengqiao Guo,Yu Xiao,Xinyu Cheng,Shuxia Liu,Xiangjun Guan,Yaokun Wu,Weihua Zhao,Guojiang Wei. Effects of Sowing Date, Density and Fertilizer Utilization Rate on the Yield of Oilseed Perilla frutescens in Cold Area[J]. Crops, 2018, 34(4): 126 -130 .
[8] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[9] Jie Gao,Qingfeng Li,Qiu Peng,Xiaoyan Jiao,Jinsong Wang. Effects of Different Nutrient Combinations on Plant Production and Nitrogen, Phosphorus and Potassium Utilization Characteristics in Waxy Sorghum[J]. Crops, 2018, 34(4): 138 -142 .
[10] Na Shang,Zhongxu Yang,Qiuzhi Li,Huihui Yin,Shihong Wang,Haitao Li,Tong Li,Han Zhang. Response of Cotton with Vegetative Branches to Plant Density in the Western of Shandong Province[J]. Crops, 2018, 34(4): 143 -148 .