Crops ›› 2017, Vol. 33 ›› Issue (1): 144-149.doi: 10.16035/j.issn.1001-7283.2017.01.026

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The Influence of Resistant and Susceptible Soybean Germplasm on Growth Dynamic Changes of Soybean Cyst Nematode Race 3（SCN3）

Li Haiyan1,Cai Deli1,Chen Jingsheng2,Duan Yuxi3,Chen Lijie3,Shang Yingyu1   

  1. 1College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163316,Heilongjiang,China
    2Daqing Branch of Heilongjiang Academy of Sciences,Daqing 163316,Heilongjiang,China
    3Nematology Institute of Northern China,College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,Liaoning,China
  • Received:2016-09-27 Revised:2016-11-04 Online:2017-02-15 Published:2018-08-26

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Abstract:

A pot experiment was conducted with resistant cultivars Wuzhai Heidou, 07-2-10-6 and susceptible cultivars Hefeng 35, 07-2-7-14. The influence of different germplasm to growth dynamic changes of soybean cyst nematode race 3（SCN3）were determined. The results indicated that root exudates had an important effect on the egg hatch of SCN3. Root exudates of Hefeng 35 and 07-2-7-14 stimulated egg incubation of SCN3, while root exudates of Wuzhai Heidou and 07-2-10-6 inhibited egg incubation of SCN 3. After incubating 10 days, the J2 hatching rate of Hefeng 35, 07-2-7-14 were 3.71 and 4.38, respectively, while the J2 hatching rate of Wuzhai Heidou, 07-2-10-6 were 0.89 and 1.39, respectively. The dynamic changes of SCN3 in soybean root indicated that the number of J2 seldom invaded into resistant cultivars. There was only a part of larva at J2 stage that could develop to J3 stage, and their growth was delayed that the ratio of female to male was 1:4.13. But there was a large number of J2 stage larva that invaded into susceptible cultivars, and most of them could develop to J3 stage. The growth speed of larva in susceptible cultivars was faster than that in resistant cultivars, and the ratio of female to male was 10.56:1. The resistant mechanism of Wuzhai Heidou to SCN 3 was integrated effects of anti-intrusion, anti-growth and anti-reproduction.

Key words: Resistant and susceptible soybean germplasm, Soybean cyst nematode, Race 3 (SCN3), Root exudates, Growth dynamic changes

Fig.1

The difference number of SCN J2 on root to hybrids and their parents The different lowercase letters represented significant differences,the same below"

Fig.2

The infuluences of hybrids and their parents root-exudates on the hatching of cyst"

Fig.3

J2 numbers of hybrids and their parents root-exudates on the hatching of cyst during 10 days"

Fig.4

The J2 hatching rate of hybrids and their parents root-exudates during 10 days"

Table 1

The J2 numbers of different age seedling root-exudates on the hatching of cyst during 10 days"

品种（系）
Varieties (lines)		 苗龄Age seedling
6d 18d 30d
五寨黑豆Wuzhai Heidou 169d 174b 134c
07-2-10-6 262c 303b 313b
合丰35 Hefeng 35 697b 1 221a 1 364a
07-2-7-14 824a 1 033a 1 432a

Table 2

The number of J2 SCN in hybrids and their parents 5 days after inoculation"

品种（系）Varieties(lines) I II III 平均值The average value
合丰35 Hefeng35 42 66 49 52.3±7.13aA
07-2-7-14 38 58 42 46.0±6.11aAB
07-2-10-6 15 32 16 21.0±5.51bAB
五寨黑豆Wuzhai Heidou 15 16 13 14.7±0.88bB

Table 3

The number of SCN race 3 at each stage in the soybean roots after inoculation different days"

品种（系）
Varieties(lines)		 6d 12d 18d 24d 30d
J2 J3 J4 J2 J3 J4 J2 J3 J4 J2 J3 J4 J2 J3 J4
五寨黑豆Wuzhai Heidou 14.7c 0.0c 0.0 39.6d 5.7d 0.0c 29.7b 14.3c 6.9d 10.4a 0.0c 17.1bc 7.3a 0.0 3.3a
合丰35 Hefeng 35 80.7b 13.4a 1.3 161.3b 31.3b 10.7ab 33.7ab 39.7b 56.3c 0.0c 3.3b 22.1b 5.3b 0.0 0.0b
07-2-10-6 53.7b 0.0c 0.0 80.6c 19.7c 6.7b 41.3a 35.3b 17.7b 3.6b 8.7a 10.0c 0.0c 0.0 3.7a
07-2-7-14 111.4a 6.1b 0.0 201.5a 47.7a 13.2a 25.5b 67.8a 73.9a 0.0c 0.0c 33.7a 0.0c 0.0 0.0b

Fig.5

The effect on female and male ratio of the SCN in the root of different soybean cultivars"

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