Crops ›› 2021, Vol. 37 ›› Issue (3): 34-39.doi: 10.16035/j.issn.1001-7283.2021.03.005

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Screening of Elite Germplasms and Identification of Seed Tocopherol and Its Component Contents in Soybean

Qin Ning(), Li Junru, Li Wenlong, Du Hui, Li Xihuan(), Zhang Caiying   

  1. Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071001, Hebei, China
  • Received:2020-06-30 Revised:2021-04-16 Online:2021-06-15 Published:2021-06-22
  • Contact: Li Xihuan E-mail:1362822834@qq.com;lixihuan@hebau.edu.cn

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Abstract:

Tocopherol (vitamin E) has many important medical functions, such as preventing and curing arteriosclerosis, lowering cholesterol, enhancing blood circulation, preventing and curing cardiovascular diseases, and so on. Soybean contains a relatively higher amount tocopherol than other plants, while need more widely elite germplasm identification and further studies. Because of this, two hundred and ninety-nine soybean varieties were analyzed for their tocopherol and its component contents through the high performance liquid chromatography (HPLC) method, and the genetic variations, correlation relationships, and elite germplasms were screened out. The results showed that it existed relative wide genetic variations of tocopherol and its components in the soybeans, and the δ-, γ-, α-, total tocopherol contents were 44.15, 143.05, 66.81, and 254.01μg/g, respectively, with the variation coefficients 15.37%-38.04%, the variation coefficients of α-tocopherol was the highest. The ratio of δ-/total, γ-/total, α-/total were 17.47%, 56.57%, and 25.96%, respectively, with the variation coefficients of 11.78%-32.21%, the ratio of α-/total was the highest. Meanwhile, highly significant correlations were detected between the total tocopherol and its three components, and the correlations between δ- and γ-, α-tocopherol were also extremely significant. Based on the contents of tocopherol and its components, the soybean varieties were divided into three groups, and thirteen elite germplasms with higher tocopherol contents were screened out, including Heihe 44, Hefeng 50, Henong 75, and Suinong 28 which can be used in the genetic improvement of tocopherol and its component contents through conventional and molecular breeding methods in the future.

Key words: Soybean, Tocopherol, High performance liquid chromatography, Elite germplasms

Table 1

Genetic variation of tocopherol and its component contents in soybean varieties"

项目
Item		 均值
Mean		 最大值
Max		 最小值
Min		 标准差
SD		 变异系数
CV (%)		 偏度
Skew		 峰度
Kurt		 品种间显著性
Significance
of varieties
δ-生育酚 δ-tocopherol (μg/g) 44.15 86.92 20.88 11.63 26.33 0.39 0.36 ***
γ-生育酚 γ-tocopherol (μg/g) 143.05 207.09 81.23 24.01 16.78 -0.04 0.05 ***
α-生育酚 α-tocopherol (μg/g) 66.81 163.49 4.38 25.42 38.04 0.56 0.81 ***
总生育酚 Total tocopherol (μg/g) 254.01 382.86 148.95 39.04 15.37 0.12 0.24 ***
δ-生育酚与总生育酚比值 δ-/total tocopherol (%) 17.47 29.62 8.23 4.08 23.37 0.22 0.03 ***
γ-生育酚与总生育酚比值 γ-/total tocopherol (%) 56.57 78.17 34.83 6.66 11.78 -0.20 1.02 ***
α-生育酚与总生育酚比值 α-/total tocopherol (%) 25.96 55.44 2.23 8.36 32.21 0.51 1.01 ***

Fig.1

The distribution of tocopherol and its component contents in soybean varieties"

Table 2

Correlation analysis of tocopherol and its component contents in soybean varieties"

项目Item γ-生育酚
γ-tocopherol		 α-生育酚
α-tocopherol		 总生育酚
Total tocopherol		 δ-生育酚比值
δ-/total tocopherol		 γ-生育酚比值
γ-/total tocopherol		 α-生育酚比值
α-/total tocopherol
δ-生育酚 δ-tocopherol 0.452** -0.156** 0.474** 0.798** 0.005 -0.394**
γ-生育酚 γ-tocopherol 0.006 0.753** -0.002 0.437** -0.347**
α-生育酚 α-tocopherol 0.608** -0.569** -0.803** 0.918**
总生育酚 Total tocopherol -0.134* -0.253** 0.267**
δ-生育酚比值 δ-/total tocopherol 0.163** -0.618**
γ-生育酚比值 γ-/total tocopherol -0.876**

Table 3

Cluster analysis of tocopherol and its component contents in soybean varieties μg/g"

类别
Type		 品种
Variety		 生育酚总量均值
Mean of total tocopherol contents
Ⅰ类：高含量
TypeⅠ：High content		 黑河44、合丰50、合农75、绥农28、合丰55、哈12-4547、辽豆26、北疆九1号、永丰豆、绥农35、圣168、宾县黑豆、绥农15、垦豆40、海6055、合农68、抗线9号、垦丰18、合交02-69、辽10Q015、合丰41、合农66、吉育48、蒙豆9号、蒙豆36、吉育101、哈14-2146、黑河41、黑河9号、哈11-4519、绥农14、绥农1号、哈13-2089、东农42、合丰53、黑河33等 319.37
Ⅱ类：低含量
TypeⅡ：Low content		 合农69、青豆、紫花2、宝青绿大豆、东农47、辽青豆1号、东农57、绿瓤黑豆、青杂豆、绥农70、佳黑秣食豆、茶秣食豆、合丰38、吉原引3号、黑豆、合农71、吉育86、合农67、北丰16 178.30
Ⅲ类：中间含量
Type Ⅲ：Medium content		 吉育204、绥农26、长春满仓金、绥08-5331、绥农52、吉育401、哈14-2028、合丰35、嫩丰16、东农50、黑河40、绥农43、合丰46、永吉枣豆、北疆193、哈13-613、黑河54、黑河38、绥农69、吉育71、绥农83、合丰44、绥农53、哈11-4142、合农97、合农76、合丰48、黑河09-3311、合丰42、合丰37、吉育108、辽小粒豆2、北豆5、黑河45、甘248、疆丰23-3412、北豆19、合农60、辽08Q104、黑河10、绥农36、华疆4403、黑河55、东农4、合农70、合丰52、合丰57、汇农10-06、黄大粒、丰收1、丰收10号、北豆36、极早黄、中黄901、丰豆23、黑河7、吉育302、蒙豆34、黑河21、黑河37、合丰56、龙垦316、绥农10号、内豆4号、东农56、黑河4、绥农11、绥农4号、大红脐、龙垦330、嫩良7号、黑河12、东农4211、牛毛黄、合丰51、呼交282、北丰9、绥农79、绥农34、黑河31、黑河43、黑河18、哈13-2185、黑河3、蒙豆28、吉育406、绥农82、绥黑大豆1号、黑河48、绥农6、舒兰满仓金、桦南小金豆、中作引1号、合丰29、绥农22等 249.98

Table 4

The soybean germplasms with high tocopherol and its component contents in seed μg/g"

品种Variety 总生育酚Total tocopherol δ-生育酚 δ-tocopherol γ-生育酚 γ-tocopherol α-生育酚 α-tocopherol
黑河44 Heihe 44 382.86 68.53 186.18 128.16
合丰50 Hefeng 50 351.36 46.67 193.58 111.11
合农75 Henong 75 350.05 54.80 186.60 108.64
绥农28 Suinong 28 347.34 54.07 196.09 97.18
合丰55 Hefeng 55 343.02 35.71 206.16 101.15
哈12-4547 Ha12-4547 338.39 86.92 178.46 73.01
哈13-2089 Ha13-2089 335.30 45.03 137.17 153.10
蒙豆9号Mengdou 9 332.78 55.27 207.09 70.42
蒙豆36 Mengdou 36 323.37 54.01 198.52 70.84
辽10Q015 Liao10Q015 316.74 75.64 178.82 62.27
东农42 Dongnong 42 303.55 37.34 125.38 140.83
绥农1号 Suinong 1 294.92 24.28 107.14 163.49
东辽克霜Dongliaokeshuang 281.72 83.46 166.13 32.13
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