Crops ›› 2021, Vol. 37 ›› Issue (3): 40-45.doi: 10.16035/j.issn.1001-7283.2021.03.006

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Identification and Correlation Analysis of Wheat Cultivars (Lines) Resistance to Leaf Blight Caused by Bipolaris sorokiniana at Seedling Stage and Filling Stage

Li Mengyu(), Gao Chuang, Li Qiaoyun(), Xu Kaige, Wang Siyu, Niu Jishan   

  1. Henan Agricultural University/National Engineering Research Centre for Wheat, Zhengzhou 450046, Henan, China
  • Received:2020-06-23 Revised:2021-03-04 Online:2021-06-15 Published:2021-06-22
  • Contact: Li Qiaoyun E-mail:untitled6002@163.com;lqylhy@163.com

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Abstract:

Wheat leaf blight is severely harmful to wheat production. Bipolaris sorokiniana is a major pathogen causing this disease. In order to screen the germplasms resistant to leaf blight caused by B.sorokiniana, the method of spraying spore suspension and covering for moisture was used at seedling stage and filling stage of 130 wheat cultivars/lines at the wheat-growing season in 2019-2020 and the correlation of resistance between seedling stage and filling stage were analyzed. The results showed that among 130 wheat cultivars (lines), resistant genotypes were 32.3% at seedling stage, of which high and moderately resistant genotypes were 1.5% and 30.8%, respectively, and had no immune genotype, susceptible germplasm was 67.7% of which moderately and high susceptible genotypes were 20.8% and 46.9%, respectively. The resistant and susceptible cultivars (lines) were 11.5% and 88.5% at filling stage, respectively, and had no high resistant genotype. There was a significant positive correlation between resistances to leaf blight caused by B. sorokiniana at seedling stage and filling stage (r=0.72). The results provide excellent resistant resources for wheat breeding and mechanism study of resistance to leaf blight. The significant correlation between resistances at seedling and filling stage provides promising evidence for evaluating resistance to leaf blight at filling stage through evaluating seedling resistance under artificial inoculation condition in the growth chamber, which is rapid and accurate and can alleviate the heavy workload of resistance identification in the field at filling stage, and reduced the impact of environmental factors on identification results.

Key words: Wheat, Leaf blight, Bipolaris sorokiniana, Resistance identification

Table 1

DLA of 130 wheat cultivars (lines) inoculated with B. sorokiniana at seedling stage and filling stage %"

品种（系）
Cultivar (line)		 苗期
Seedling stage		 灌浆期
Filling stage		 品种（系）
Cultivar (line)		 苗期
Seedling stage		 灌浆期
Filling stage		 品种（系）
Cultivar(line)		 苗期
Seedling stage		 灌浆期
Filling stage
新矮早818 25.7 50.8 11-695(LWX) 66.6 60.1 中国春 17.3 44.0
新麦13 16.0 11.2 11-696(LWX) 52.8 54.2 远嫁69 12.6 42.5
石L5206-10 18.2 52.5 SP1777-1-4 66.8 81.7 YN177 20.5 44.0
陕优225 11.7 21.8 郑麦366 68.9 63.8 481/274 65.1 87.5
矮丰66 20.2 32.7 绵资02-12 48.8 67.5 TA 3809/312 44.6 57.5
荷兰大籽 14.4 43.0 11-252(LWX) 75.8 71.3 徐麦9169 16.4 39.3
C50232 16.2 55.5 11-253(LWX) 65.9 69.2 囤麦127 19.6 48.8
豫麦47 21.0 58.8 11-269(LWX) 76.8 87.6 百农107 20.3 55.8
郑2062 10.4 58.0 天02-204 68.9 97.7 辉县红 55.1 76.7
良星99 14.6 25.0 12家2联 60.8 78.4 荆辉1号 61.4 50.4
新麦1817 27.7 25.4 济麦21 44.6 56.7 扬麦5号 52.7 65.0
偃展4110 55.0 75.0 CA0816 40.4 57.4 Chancellor 61.2 62.3
陇麦135 41.4 45.0 国麦0116 55.9 66.7 Yuma 26.5 35.0
百泉41 36.0 78.4 高冬2 33.7 76.3 92R137 47.8 73.8
西农9871-1 52.2 82.3 淮核0308 41.4 75.1 11YC173 23.4 25.8
阜麦936 52.3 66.7 鹤0927 20.1 33.4 抗线虫1-1 55.4 66.3
碧蚂1号 49.0 44.8 单体 20.8 65.0 抗线虫1-4 61.5 55.0
岗01 19.7 30.0 许科316 20.6 58.6 温麦8号 66.8 80.4
川农18 19.8 37.2 驻麦6097 12.9 46.7 温麦10号 79.2 97.5
周19 30.9 60.0 航麦901 66.0 80.2 57048 20.1 68.3
节燕98-7 19.5 35.4 Wheatear 28.1 63.3 65498 24.4 25.0
04ZP16 50.0 63.8 Guomai222 27.4 45.8 鄂麦170 23.1 11.4
10M8 17.1 31.7 BP57-1 26.1 43.8 鄂麦526 27.9 50.0
10M14 47.4 60.5 望水白 56.1 52.8 珍麦3号 76.1 22.9
10M24 8.9 16.3 苏麦3号 45.3 63.8 涡麦66 65.2 37.6
10M31 31.4 67.0 PH691 38.2 60.0 中麦170 16.0 21.0
10M16 22.5 67.5 Tybalt 15.6 28.0 郑麦132 13.5 19.5
10M21 20.8 55.0 丽麦16 60.1 84.4 丰德存麦16 27.9 43.8
10M23 24.7 23.3 济南31 33.3 51.7 CIM-1 36.4 58.3
2101-136 28.8 59.2 品质所材料6 12.5 36.3 CIM-17 40.0 48.3
郑州991 28.9 38.0 宛原白1号 18.9 23.6 秋乐2132 51.8 68.4
国麦2号 75.2 48.5 济程2号 14.0 25.4 郑麦1354 54.6 67.5
CH-1 63.4 84.1 豫优1号 28.9 66.3 中育1526 50.2 73.1
西农979-5 18.4 31.0 石98-7136 60.0 83.8 扶麦368 49.9 64.4
04高春4 59.0 48.0 山农4143 77.7 89.4 瑞华1426 41.1 60.7
山农530070 67.3 70.0 SP1777-6-8 65.1 90.0 浚麦118 28.3 42.2
09X1 24.7 33.5 11-285 48.6 86.5 宁麦13 25.4 45.7
PIC420 52.3 96.6 283 32.3 60.5 浩麦1号 68.6 66.3
G57(LHL) 41.8 39.6 10M18 26.9 35.0 淮麦28 57.7 75.0
11-229(LWX) 11.4 52.8 11-270 69.9 83.3 烟农19 53.9 54.4
11-239(LWX) 36.6 67.5 YN006 29.7 58.8 安农0711 32.5 37.5
11-358(LWX) 36.5 40.0 L01378 28.0 12.8 安农1589 33.2 59.0
11-504(LWX) 59.6 62.5 L661(LPG) 61.8 63.0
11-411(LWX) 62.5 65.0 山农737 29.2 31.0

Table 2

Resistance evaluation of 130 wheat cultivars (lines) to leaf blight caused by B. sorokiniana at seedling stage and filling stage"

DLA范围
Range of
DLA (%)		 抗性评价
Resistance
evaluation		 苗期Seedling stage 灌浆期Filling stage
品种（系）数量（占比）
Number of cultivars (lines) (percentage)		 平均DLA
Average DLA		 品种（系）数量（占比）
Number of cultivars (lines) (percentage)		 平均DLA
Average DLA
1.0~10.9 高抗 2(1.5%) 9.7C - -
11.0~25.9 中抗 40(30.8%) 18.6C 15(11.5%) 20.7C
26.0~40.0 中感 27(20.8%) 30.8B* 19(14.6%) 34.8B*
>40.0 高感 61(46.9%) 57.1A* 96(73.9%) 64.1A*

Fig.1

Performance of wheat cultivars (lines) resistant and susceptible to leaf blight caused by B. sorokiniana at seedling and filling stage R=resistant, S=susceptible. Bs and CK represent the samples inoculated with B. sorokiniana and those treated by distilled water (control), respectively. The resistant materials (a) are Xuke 316, PIC420, Yuanjia 69, PH691 and Zhumai 6097, and the susceptible materials (b) are Xinaizao 818, Bima1, Wangshuibai, 11-253(LWX) and Huixianhong at seedling stage. At filling stage, the resistant and susceptible materials are Emai 170 (c) and Xinong 9871-1 (d), respectively"

Fig.2

Correlation analysis of disease leaf area (DLA) between seedling stage and filling stage"

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