Crops ›› 2023, Vol. 39 ›› Issue (4): 237-244.doi: 10.16035/j.issn.1001-7283.2023.04.034

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Effects of Superimposed Application of BR in Different Periods on Yield and Quality of Sugar Beet under Saline-Alkali Stress

Zhang Hanwen(), Liu Dan, Wang Xuerui, Li Wangshu, Lu Qiang, Wang Shufeng, Zhao Jianan, Wang Yubo, Zhang He, Li Caifeng()   

  1. College of Agriculture, Northeast Agricultural University, Harbin 150038, Heilongjiang, China
  • Received:2022-03-11 Revised:2022-03-28 Online:2023-08-15 Published:2023-08-15

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Abstract:

In order to explore the effects of superimposed spraying of brassinolide (BR) in different periods on improving the stress resistance of sugar beet under saline-alkali stress, the sugar beet variety KWS0143 was used as the test material to conduct a simulated saline-alkali experiment in barrels. A total of five treatments were set, spraying water treatment (CK), and superimposed spraying BR in the periods of leaf clump formation and tuberous root expansion (QZ), superimposed spraying BR in the periods of tuberous root expansion and sugar accumulation (ZH), superimposed spraying BR in the periods of foliar clump formation and sugar accumulation (QH), and superimposed spraying BR in the periods of leaf clump formation, tuberous root expansion and sugar accumulation under saline-alkali stress (QZH). The results showed that the contents of photosynthetic pigments, photosynthetic fluorescence parameters and RuBP carboxylase activity after BR treatments were improved compared with CK. The contents of betaine, amino acids, protein and nitrate ions and reducing sugar were lower than that of CK, and the sucrose content was higher than that of CK, and the effects of QZH treatment was the best. Compared with the CK treatment, the tuberous root yields of the QH and QZ treatments were increased by 12.58% and 9.88%, respectively, the sugar contents of the QZH and QZ treatments were increased by 5.1 and 4.6 degrees, respectively, and the sugar yield of the QZH treatment was increased by 12.79%. QZH treatment could effectively reduce the accumulation of non-sugar substances in the beet tuber, improve the quality of the tuberous root, and increase the sugar yield of the sugar beet.

Key words: Sugar beet, Salt-alkali stress, Photosynthetic index, BR, Quality

Table 1

Effects of superimposed spraying of BR on RuBP carboxylase activities in different periods μmol/(mL?h)"

处理
Treatment		 取样日期（月-日）Sampling date (month-day)
07-04 07-22 08-10 08-28 09-15
CK 80.722±0.078e 82.579±0.288d 108.153±0.040e 109.400±0.118d 141.553±0.251b
QZ 87.643±0.045c 89.521±0.207b 115.547±0.321c 117.316±0.236b 131.515±0.278d
ZH 81.698±0.463d 84.289±0.240c 110.572±0.058d 115.491±0.292e 129.312±0.161e
QH 88.566±0.208b 89.557±0.343b 182.645±0.305a 193.505±0.321a 207.285±0.207a
QZH 89.772±0.015a 91.654±0.300a 119.583±0.360b 116.631±0.264c 138.641±0.300c

Table 2

Effects of superimposed spraying of BR on chlorophyll a contents in different periods mg/g FW"

处理
Treatment		 取样日期（月-日）Sampling date (month-day)
07-04 07-22 08-10 08-28 09-15
CK 0.81±0.01b 0.56±0.01b 0.95±0.05a 0.58±0.02d 0.52±0.01b
QZ 1.17±0.09a 0.79±0.25a 0.87±0.25ab 0.76±0.04ab 0.72±0.05a
ZH 0.88±0.07b 0.48±0.03b 0.66±0.00b 0.65±0.01cd 0.78±0.07a
QH 1.13±0.04a 0.96±0.07a 0.76±0.08ab 0.70±0.06bc 0.45±0.04b
QZH 1.16±0.02a 1.00±0.09a 0.95±0.16a 0.78±0.04a 0.81±0.08a

Table 3

Effects of superimposed spraying of BR on chlorophyll b contents in different periods mg/g FW"

处理
Treatment		 取样日期（月-日）Sampling date (month-day)
07-04 07-22 08-10 08-28 09-15
CK 0.17±0.01b 0.12±0.01a 0.15±0.01a 0.07±0.00b 0.09±0.00c
QZ 0.26±0.02a 0.19±0.03a 0.14±0.02a 0.15±0.02a 0.13±0.01b
ZH 0.17±0.02b 0.13±0.07a 0.14±0.05a 0.14±0.02a 0.15±0.02a
QH 0.24±0.03a 0.18±0.04a 0.12±0.02a 0.12±0.04a 0.15±0.01a
QZH 0.26±0.01a 0.13±0.12a 0.15±0.01a 0.16±0.01a 0.16±0.01a

Table 4

Effects of superimposed spraying of BR on carotenoid contents in different periods mg/g FW"

处理
Treatment		 取样日期（月-日）Sampling date (month-day)
07-04 07-22 08-10 08-28 09-15
CK 0.26±0.01b 0.16±0.01b 0.28±0.02ab 0.23±0.01b 0.17±0.01b
QZ 0.37±0.02a 0.30±0.01a 0.28±0.02ab 0.26±0.01a 0.23±0.03a
ZH 0.27±0.01b 0.16±0.02b 0.21±0.01c 0.20±0.01c 0.16±0.02b
QH 0.36±0.01a 0.30±0.02a 0.24±0.02bc 0.23±0.01b 0.26±0.02a
QZH 0.38±0.02a 0.33±0.02a 0.30±0.04a 0.27±0.02a 0.27±0.02a

Table 5

Effects of superimposed spraying of BR on the YⅡ of leaves in different periods"

处理
Treatment		 取样日期（月-日）Sampling date (month-day)
07-04 07-22 08-10 08-28 09-15
CK 0.57±0.02c 0.66±0.02c 0.65±0.05ab 0.65±0.04a 0.37±0.01d
QZ 0.63±0.01a 0.75±0.01a 0.65±0.02ab 0.67±0.11a 0.46±0.04c
ZH 0.60±0.01b 0.68±0.02bc 0.68±0.02ab 0.70±0.02a 0.52±0.01b
QH 0.63±0.02a 0.71±0.03b 0.63±0.01b 0.71±0.03a 0.52±0.01b
QZH 0.66±0.04a 0.77±0.04a 0.68±0.03a 0.76±0.03a 0.58±0.02a

Table 6

Effects of superimposed spraying of BR on leaf qL in different periods"

处理
Treatment		 取样日期（月-日）Sampling date (month-day)
07-04 07-22 08-10 08-28 09-15
CK 0.65±0.01b 0.59±0.01b 0.65±0.10a 0.46±0.00c 0.28±0.10c
QZ 0.97±0.02a 0.66±0.03a 0.65±0.03a 0.76±0.04a 0.46±0.02b
ZH 0.64±0.01b 0.60±0.01b 0.61±0.01a 0.54±0.01b 0.56±0.01ab
QH 0.97±0.01a 0.64±0.02a 0.63±0.01a 0.50±0.01bc 0.51±0.02ab
QZH 1.05±0.10a 0.67±0.03a 0.67±0.01a 0.77±0.07a 0.60±0.07a

Table 7

Effects of superimposed BR spraying on leaf NPQ in different periods"

处理
Treatment		 取样日期（月-日）Sampling date (month-day)
07-04 07-22 08-10 08-28 09-15
CK 0.22±0.01a 0.12±0.01a 0.07±0.01a 0.22±0.04a 0.26±0.04a
QZ 0.21±0.01a 0.10±0.01b 0.08±0.01a 0.19±0.03a 0.21±0.02b
ZH 0.22±0.02a 0.10±0.01b 0.07±0.01a 0.22±0.04a 0.23±0.02ab
QH 0.21±0.01a 0.10±0.01b 0.08±0.00a 0.21±0.02a 0.23±0.01ab
QZH 0.15±0.01b 0.08±0.01c 0.07±0.01a 0.18±0.01a 0.21±0.01b

Table 8

Correlation analysis of photosynthetic parameters and parameters of photosystem II"

指标
Parameter		 叶绿素a含量
Chlorophyll a content		 叶绿素b含量
Chlorophyll b content		 类胡萝卜素含量
Carotenoid content		 YⅡ qL NPQ
叶绿素a含量Chlorophyll a content 1.000
叶绿素b含量Chlorophyll b content 0.257 1.000
类胡萝卜素含量Carotenoid content 0.870** 0.412 1.000
YⅡ 0.661** 0.030 0.834** 1.000
qL 0.644** 0.087 0.828** 0.897** 1.000
NPQ -0.528* -0.099 -0.663** -0.691** -0.498 1.000

Fig.1

Effects of superimposed spraying BR on betaine contents in different periods Different lowercase letters indicate significant difference between treatments at the P < 0.05 level, the same below"

Fig.2

The effects of superimposed spraying BR on the contents of amino acid and protein in different periods"

Fig.3

Effects of superimposed spraying of BR on NO3- contents in different periods"

Fig.4

Effects of superimposed spraying BR on the contents of reducing sugar and sucrose in different periods"

Table 9

Effects of superimposed spraying of BR on root yield, sugar contents and sugar yield of sugar beet in different periods"

处理
Treatment		 块根产量（g/株）
Tuberous root yield
(g/plant)		 含糖率（度）
Sugar content
(degree)		 产糖量（g/株）
Sugar yield
(g/plant)
CK 210.69±6.21b 12.50±0.40b 26.35±1.62b
QZ 231.50±7.90a 17.10±0.90a 39.63±3.43a
ZH 211.10±7.50b 13.90±1.30b 29.41±3.79b
QH 237.20±8.90a 16.30±0.90a 38.72±3.59a
QZH 226.70±5.80a 17.60±0.30a 39.91±1.70a
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