Crops ›› 2023, Vol. 39 ›› Issue (6): 54-61.doi: 10.16035/j.issn.1001-7283.2023.06.008

Previous Articles     Next Articles

Comprehensive Evaluation of Genetic Diversity in 70 Barley Germplasms

Zhao Feng(), Bao Qijun(), Pan Yongdong, Liu Xiaoning, Zhang Huayu, Niu Xiaoxia   

  1. Institute of Economic Crop and Beer Material, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China
  • Received:2023-03-03 Revised:2023-07-26 Online:2023-12-15 Published:2023-12-15

RichHTML

6

PDF (PC)

117

Abstract:

In order to explore the genetic diversity of barley germplasm resources, and elect the barley germplasms with comprehensive excellent traits, 70 barley germplasms were employed as materials, correlation analysis, cluster analysis and principal component analysis of ten main agronomic traits for three consecutive years were used to analyze genetic diversity and comprehensive evaluation. The results showed that there were abundant genetic variations in barley resources, and coefficient of variation of the first internode length was largest (56.24%), followed by grain number per spike (44.31%) and grain weight per spike (41.50%). The tested barley were divided into six groups by cluster analysis, the groupⅠbelonged to long rod-short spike type and could be used for breeding of both food and feed varieties; the groupⅡbelonged to large grain-high yield type with best comprehensive traits could be used for breeding of dwarf-large grain-high yield varieties or used directly in production; the group Ⅲ could be used for breeding of long spike varieties; the group Ⅳ could be used for breeding of dwarf-more spike variety; the group Ⅴ with worst comprehensive traits was recommended to be preserved as resource materials recommended for breeding, the group Ⅵ belonged to more spike-small grain. Based on the analysis of principal components and the scatter plot, three excellent types of germplasms were obtained, which behaved dwarf, strong tillering ability and high production, meanwhile they contained three types (short spike, more grain and long spike), respectively, and the comprehansive evaluation equation of barley germplasms was established.

Key words: Barley, Germplasm resource, Agronomic traits, Genetic diversity

Table 1

Information of barley germplasm for tested"

编号Number 名称Name 全国统一编号National uniform 编号Number 名称Name 全国统一编号National uniform
1 C2 － 36 云啤18号 ZDM10176
2 中糯8号 － 37 凤大麦3号 －
3 SV255 － 38 凤大麦4号 －
4 Tradition WDM07429 39 凤大麦6号 ZDM09985
5 中川大麦 － 40 凤大麦7号 ZDM09986
6 Hiproly WDM3169 41 凤大麦9号 ZDM10182
7 Jliprocy WDM3173 42 凤大麦10号 ZDM10183
8 Prokǔpkǔr WDM3188 43 凤大麦11号 ZDM10184
9 H.dist.var.nigx.xtrif WDM689 44 凤大麦12号 ZDM10185
10 京裸6号 ZDM8256 45 凤大麦13号 ZDM10186
11 城武芒大麦 ZDM00279 46 凤大麦14号 ZDM10187
12 红青稞 ZDM1112 47 凤大麦17号 ZDM10188
13 六棱大麦 ZDM1158 48 凤03-39 ZDM09988
14 皮大麦 ZDM4214 49 凤V-43 －
15 六棱青稞 ZDM5110 50 黄青1号 ZDM10213
16 白青稞 ZDM5111 51 甘青4号 ZDM10209
17 禄劝25 ZDM5439 52 甘青6号 ZDM10211
18 春青稞 ZDM6616 53 甘青7号 ZDM10212
19 毛大麦 ZDM7787 54 昆仑14 ZDM10215
20 城固大麦 ZDM7808 55 禾尚青稞 ZDM07842
21 长芒青稞 ZDM7821 56 肚里黄 ZDM08341
22 老大麦 ZDM7823 57 西308 －
23 长芒大麦 ZDM897 58 保大麦8号 ZDM10190
24 卷芒大麦 ZDM981 59 保大麦12号 ZDM10191
25 城武芒大麦 ZDM 279 60 保大麦13号 ZDM10192
26 中品1777 ZYM1722 61 保大麦14号 ZDM10193
27 藏3363 ZYM3393 62 保大麦15号 ZDM10194
28 藏3369 ZYM3396 63 保大麦16号 ZDM10195
29 藏3370 ZYM3397 64 保大麦18号 ZDM10197
30 藏0060 ZYM4216 65 保大麦19号 ZDM10198
31 中品750 ZYM742 66 保大麦20号 ZDM10265
32 云啤2号 ZDM09977 67 保大麦21号 ZDM10266
33 云啤9号 ZDM10163 68 保大麦22号 ZDM10267
34 云啤10号 ZDM10164 69 保大麦23号 ZDM10268
35 云啤15号 ZDM10174 70 甘啤6号 ZDM10062

Table 2

Variation analysis of main agronomic traits of barley germplasm resources"

性状Trait 最小值
Minimum value		 最大值
Maximum value		 平均值
Average value		 标准差
SD		 变异系数
CV (%)
株高Plant height (cm) 53.00 117.70 82.83 13.53 16.33
穗长Spike length (cm) 5.00 13.30 7.60 1.50 19.74
第一茎节长The first internode length (cm) 1.00 9.10 2.75 1.54 56.24
第二茎节长The second internode length (cm) 3.10 14.20 6.56 2.66 40.58
穗下茎节长Internode length below spike (cm) 16.00 41.20 29.00 7.16 24.70
单株穗数Spike number per plant 1.60 5.50 3.43 0.74 21.72
单穗粒重Grain weight per spike (g) 0.60 3.20 1.47 0.61 41.50
主穗粒数Grain number per spike 14.00 72.00 38.24 16.95 44.31
千粒重1000-grain weight (g) 31.40 50.40 42.47 4.42 10.41
小区产量Yield per plot (kg) 0.28 1.19 0.70 0.21 30.00

Table 3

Correlation analysis of main agronomic of barley germplasm resources"

性状
Trait		 株高
Plant
height		 穗长
Spike
length		 第一茎节长
The first
internode length		 第二茎节长
The second
internode length		 穗下茎节长
Internode
length
below spike		 单株穗数
Spike
number
per plant		 单穗粒重
Grain
weight
per spike		 主穗粒数
Grain
number
per spike		 千粒重
1000-
grain
weight		 小区
产量
Yield
per plot
株高Plant height 1.000
穗长Spike length 0.371** 1.000
第一茎节长The first internode length 0.436** 0.638** 1.000
第二茎节长The second internode length 0.500** 0.602** 0.881** 1.000
穗下茎节长Internode length below spike 0.661** -0.081 0.093 0.060 1.000
单株穗数Spike number per plant -0.458** -0.237* -0.196 -0.244* -0.434** 1.000
单穗粒重Grain weight per spike -0.022 0.095 0.112 0.160 -0.104 -0.360** 1.000
主穗粒数Grain number per spike 0.162 0.118 -0.023 0.051 0.218 -0.681** 0.649** 1.000
千粒重1000-grain weight -0.422** -0.100 -0.003 -0.100 -0.343** 0.400** 0.214 -0.288* 1.000
小区产量Yield per plot -0.502** -0.253* -0.248* -0.227 -0.530** 0.293* 0.247* -0.004 0.524** 1.000

Fig.1

Clustering diagram of barley germplasm resources"

Table 4

Principal component analysis of main agronomic traits of barley germplasm resources"

性状Trait PC1 PC2 PC3 PC4
株高Plant height 0.83 -0.11 -0.21 0.27
穗长Spike length 0.59 0.56 0.01 -0.33
第一茎节长
The first internode length		 0.65 0.67 -0.09 0.11
第二茎节长
The second internode length		 0.69 0.62 -0.03 0.05
穗下茎节长
Internode length below spike		 0.57 -0.55 -0.21 0.51
单株穗数Spike number per plant -0.69 0.33 -0.44 0.05
单穗粒重Grain weight per spike 0.16 0.12 0.89 0.15
主穗粒数Grain number per spike 0.41 -0.34 0.78 -0.14
千粒重1000-grain weight -0.53 0.50 0.21 0.54
小区产量Grain yield per plot -0.63 0.28 0.47 0.12
特征值Eigenvalue 3.60 2.03 1.95 0.81
贡献率Contribution rate (%) 35.96 20.34 19.45 8.07
累计贡献率
Cumulative contribution rate (%)		 35.96 56.30 75.75 83.83

Fig.2

Scatter plots based on principal components of barley germplasm resources"

[1] 柳小宁, 潘永东, 包奇军, 等. 剪掉倒三叶对啤酒大麦产量和品质的影响. 河南农业科学, 2021, 50(7):23-29.
[2] Cockram J, Chiapparino E, Taylor S A, et al. Haplotype analysis of vernalization loci in European barley germplasm reveals novel VRN-H1 alleles and a predominant winter VRN-H1/VRN-H2 multi- locus haplotype. Theoretical and Applied Genetics, 2007,115,993-1001.
[3] 蒋莹, 常蕾, 王安, 等. 143份大麦种质资源主要农艺性状遗传多样性分析. 江苏农业科学, 2020, 48(14):94-98.
[4] Sun L J, Lu W, Zhang J, et al. Investigation of barley germplasm in China. Genetic Resources and Crop Evolution, 1999, 46:361- 369.
doi: 10.1023/A:1008606613602
[5] 牛小霞, 柳小宁, 潘永东, 等. 97份大麦种质资源农艺性状分析与评价. 种子, 2021, 40(8):68-72.
[6] 秦丹丹, 杜静, 许甫超, 等. 基于SSR标记的大麦种质资源遗传多样性分析. 大麦与谷类科学, 2020, 37(6):1-8.
[7] 刘燕, 强小林, 黄静, 等. 中国大麦育成品种(系)的遗传多样性. 应用与环境生物学报, 2013, 19(1):79-83.
[8] 张俊英, 阮景军, 薛文韬, 等. 以色列野生二棱大麦遗传多样性分析. 分子植物育种, 2017, 15(8):3309-3317.
[9] 张蓝天, 楚单单, 方云霞, 等. 世界大麦种质资源遗传多样性分析. 杭州师范大学学报(自然科学版), 2020, 19(3):297-303.
[10] 刘亚楠, 朱娟, 吕超, 等. 二棱大麦种质的综合评价. 麦类作物学报, 2018, 38(4):430-439.
[11] 张万霞, 孙立军, 张京, 等. 优异大麦种质资源的抗性鉴定和评价. 植物遗传资源科学, 2001(3):18-21.
[12] 张小燕, 张跃进, 庞有强. 日本不同棱型大麦种质资源农艺性状的差异. 西北农业学报, 2009, 18(6):122-126.
[13] 刘亚楠, 潘雨涵, 郭晖, 等. 六棱大麦种质表型遗传多样性评价. 植物遗传资源学报, 2018, 19(5):846-856.
[14] 田朋佳, 廖文华, 高小丽, 等. 140份西藏大麦种质资源遗传多样性分析. 西南农业学报, 2022, 35(1):16-26.
[15] 张京, 刘旭. 大麦种质资源描述规范和数据标准. 北京: 中国农业出版社, 2006.
[16] 黄金堂. 二棱大麦茎秆性状与抗倒性关系的研究. 麦类作物学报, 2004, 24(3):49-52.
[17] 周元成, 曹永立, 王镇, 等. 不同大麦品种抗旱性鉴定指标的筛选与评价. 中国农业科技导报, 2022, 24(2):86-92.
doi: 10.13304/j.nykjdb.2020.0825
[18] 徐银萍, 潘永东, 张廷红, 等. 30份大麦种质资源的苗期抗旱性鉴定及抗旱指标筛选. 甘肃农业科技, 2021, 52(9):56-67.
[19] 徐银萍, 潘永东, 刘强德, 等. 大麦种质资源成株期抗旱性鉴定及抗旱指标筛选. 作物学报, 2020, 46(3):448-461.
doi: 10.3724/SP.J.1006.2020.91031
[20] 张英虎, 陈和, 陈健, 等. 2007-2014年江苏沿海地区大麦品种(系)产量与产量构成分析. 浙江农业学报, 2015, 27(9):1510-1513.
[21] Khodadadi M, Fotokian M H, Miransari M. Genetic diversity of wheat (Triticum aestivum L.) genotypes based on cluster and principal component analyses for breeding strategies. Australian Journal of Crop Science, 2011, 5(6):17-24.
[22] 朱衣成, 吴欣, 周玉琴. 超高产小麦新品种选育的主要策略. 农业科技通讯, 2012, 489(9):125-127.
[23] 张万霞, 张京, 孙立军, 等. 大麦优异种质农艺性状鉴定和适应性分析. 植物遗传资源科学, 2002(2):24-27.
[24] 马艳明, 赵连佳, 王威, 等. 大麦种质资源表型性状遗传差异分析. 新疆农业科学, 2022, 59(11):2628-2636.
doi: 10.6048/j.issn.1001-4330.2022.11.004
[25] 白盼, 李荣华, 郭培国, 等. 19个亚洲国家大麦种质材料的遗传多样性分析. 麦类作物学报, 2012, 32(2):215-222.
[26] Kolodinska B A, von Bothmer R, Dayteg C, et al. Genetic diversity changes and relationships in spring barley (Hordeum vulgare L.) germplasm of Nordic and Baltic areas as shown by SSR markers. Genetic Resources and Crop Evolution, 2007, 54:749-758.
doi: 10.1007/s10722-006-9159-4
[27] Chen Z W, Lu R J, Zou L, et al. Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China. Genetics and Molecular Research, 2012, 11(1):644-650.
doi: 10.4238/2012.March.16.2 pmid: 22535400
[28] 要燕杰, 高翔, 吴丹, 等. 小麦农艺性状与品质特性的多元分析与评价. 植物遗传资源学报, 2014, 15(1):38-47.
[29] 陈书霞, 周静, 申晓青, 等. 大蒜种质产量和品质性状主成分聚类分析与综合评价. 植物遗传资源学报, 2012, 13(3):429-434.
doi: 10.13430/j.cnki.jpgr.2012.03.017
[30] 江桐欣, 李晓琳, 朱庆锋, 等. 基于同源重组的CRISPR精准基因编辑技术及其在农作物育种中的应用. 广东农业科学, 2022, 49(11):119-127.
[1] Xia Xue, Cai Kangfeng, Liu Lei, Song Xiujuan, Wang Junmei, Yue Wenhao. Research Progress of Barley Response to Low Phosphate Stress in Root Morphology and Molecular Level [J]. Crops, 2023, 39(6): 11-16.
[2] Zhou Qilong. Screening of Suitable Mixed Sowing Methods for Annual Forage Crops in Rikaze Alpine Valley Region [J]. Crops, 2023, 39(6): 209-217.
[3] Qu Zhihua, Zhang Lili, Hu Yang, Qiao Haiming, Li Feng, Bai Wei. Agronomic Characteristics Evaluation on Introduced Flax Germplasm Resources [J]. Crops, 2023, 39(6): 47-53.
[4] Yang Enze, Wang Shuyan, Liu Ruixiang, Shi Fengyuan, Zhang Jinhao, Li Jiana, Li Zhiwei, Guo Zhanbin. Genetic Diversity Analysis of Quinoa Germplasm Resources Based on SRAP [J]. Crops, 2023, 39(6): 79-85.
[5] Yang Mei, Yang Weijun, Gao Wencui, Jia Yonghong, Zhang Jinshan. Effects of Combined Application of Biochar and Nitrogen Fertilizer on Dry Matter Transport, Agronomic Characteristics and Yield of Winter Wheat in Irrigation Area [J]. Crops, 2023, 39(5): 138-144.
[6] Liu Hui, Long Xueyi, Jiao Yan, Wang Lihong. Effects of Combined Application of Biochar and Phosphate Fertilizer on Rice Growth and Yield [J]. Crops, 2023, 39(5): 238-248.
[7] Zhang Shangpei, Yang Junxue, Luo Shiwu, Wang Yong, Zhang Xiaojuan, Cheng Bingwen. Genetic Diversity and Yielding Ability Analysis of Agronomic Traits in Broom Corn Millet [J]. Crops, 2023, 39(5): 37-42.
[8] Ge Changbin, Qin Suyan, Qiao Jiliang, Wang Jun, Qi Shuangli, Lu Wenying, Zhang Zhenyong. Comparative Analysis of Agronomic Traits, Quality and Disease Evolution of Approved Wheat Varieties in Southern Henan and Southern Huai River in Jiangsu from 2001 to 2021 [J]. Crops, 2023, 39(5): 49-58.
[9] Gao Zhanning, Yang Yongqian, Wang Shujie, Feng Hui, Xue Zhenggang. Comprehensive Evaluation of 143 Barley Germplasm Resources [J]. Crops, 2023, 39(5): 59-65.
[10] ChenZhikai , Hou Wanwei. Evaluation and Selection of Pisumsativum L. Germplasm Resources Based on Agronomic Traits [J]. Crops, 2023, 39(4): 38-43.
[11] Li Qingfeng, Gao Jie, Peng Qiu. Genetic Diversity Analysis of Agronomic and Quality Characteristics of Amaranthus Resources in Guizhou Province [J]. Crops, 2023, 39(4): 60-64.
[12] Zhao Yun, Feng Guojun, Hu Xiangwei, Wumaierjiang·Kuerban , Li Pengbing, Li Cuimei, Akebota·Muheyati . Preliminary Report on Selection of Herbicide-Resistant Foxtail Millet Varieties Suitable for Planting in Kashgar, Xinjiang [J]. Crops, 2023, 39(3): 126-133.
[13] Lu Yingji, Yang Xiaomeng, Pu Xiaoying, Li Xia, Yang Liʼe, Yang Yanbin, Zeng Yawen. Effects of Sowing and Seedling Cutting in Different Seasons on Agronomic Characteristics of Superior Barley Varieties [J]. Crops, 2023, 39(3): 215-220.
[14] Chen Cuiping, Yan Dianhai, Zhang Shumiao, Zuo Haonan, Gao Sen, Liu Yang. Fingerprint Construction and Genetic Diversity Analysis of Quinoa Based on SSR Markers [J]. Crops, 2023, 39(3): 35-42.
[15] Ma Ruiqi, Wang Demei, Tao Zhiqiang, Wang Yanjie, Yang Yushuang, Zhao Guangcai, Chang Xuhong. Effects of Topdressing Nitrogen Amount on Yield and Agronomic Traits of Different Gluten Type Wheat Cultivars [J]. Crops, 2023, 39(2): 131-137.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!