Crops ›› 2023, Vol. 39 ›› Issue (6): 79-85.doi: 10.16035/j.issn.1001-7283.2023.06.011

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Genetic Diversity Analysis of Quinoa Germplasm Resources Based on SRAP

Yang Enze1(), Wang Shuyan1(), Liu Ruixiang2, Shi Fengyuan1, Zhang Jinhao1, Li Jiana1, Li Zhiwei1, Guo Zhanbin3   

  1. 1Agricultural College of Inner Mongolia Agricultural University, Hohhot 010019, Inner Mongolia, China
    2School of Desert Control, Inner Mongolia Agricultural University, Hohhot 010019, Inner Mongolia, China
    3Inner Mongolia Yiji Biotechnology Co., Ltd., Hohhot 010010, Inner Mongolia, China
  • Received:2022-06-16 Revised:2022-07-22 Online:2023-12-15 Published:2023-12-15

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Abstract:

SRAP molecular markers were used to analyze the genetic diversity of 60 quinoa germplasms. The results showed that 60 specific bands were amplified by 11 pairs of SRAP primers, and the average polymorphic information content (PIC) of primers was 0.3199, the average number of alleles (Na) was 1.9375, the average number of effective alleles (Ne) was 1.5384, the mean value of Nei's gene diversity index (H′) was 0.3135, the average Shannon information index (I) was 0.4711. The average genetic similarity coefficient of 60 quinoa germplasm materials was 0.6748, and 60 quinoa test materials could be divided into four categories and nine sub-types when the genetic similarity coefficients were 0.69 and 0.72, respectively.

Key words: Quinoa, Germplasm resources, SRAP molecular marker, Genetic diversity

Table 1

Quinoa material for test"

编号
Number		 材料名称
Material
name		 主穗紧凑程度
Compactness
of main ear		 花序类型
Inflorescence
type		 株型
Plant
type		 产量类型
Yield
type		 编号
Number		 材料名称
Material
name		 主穗紧凑程度
Compactness
of main ear		 花序类型
Inflorescence
type		 株型
Plant
type		 产量类型
Yield
type
1 21-Y-633 中间型 圆锥 帚型，矮秆 高产 31 21-Y-766 中间型 圆锥 扇型，矮秆 高产
2 21-Y-642 松散型 圆锥 扇型，矮秆 低产 32 21-Y-770 中间型 圆锥 帚型，矮秆 高产
3 21-Y-644 松散型 总状 帚型，矮秆 低产 33 21-Y-772 松散型 圆锥 筒型，矮秆 低产
4 21-Y-662 中间型 圆锥 扇型，矮秆 低产 34 21-Y-776 中间型 圆锥 帚型，矮秆 低产
5 21-Y-663 中间型 圆锥 扇型，矮秆 高产 35 21-Y-782 中间型 圆锥 帚型，矮秆 低产
6 21-Y-666 中间型 圆锥 帚型，矮秆 低产 36 21-Y-788 中间型 圆锥 帚型，高秆 高产
7 21-Y-667 中间型 圆锥 帚型，矮秆 高产 37 21-Y-798 中间型 圆锥 帚型，高秆 高产
8 21-Y-668 中间型 圆锥 帚型，矮秆 低产 38 21-Y-801 中间型 圆锥 帚型，高秆 高产
9 21-Y-669 中间型 圆锥 帚型，矮秆 高产 39 21-Y-802 中间型 圆锥 扇型，矮秆 高产
10 21-Y-671 中间型 圆锥 帚型，矮秆 高产 40 21-Y-803 中间型 圆锥 帚型，矮秆 低产
11 21-Y-673 紧凑型 圆锥 扇型，高秆 高产 41 21-Y-805 中间型 圆锥 帚型，矮秆 低产
12 21-Y-676 松散型 总状 扇型，矮秆 低产 42 21-Y-806 中间型 圆锥 扇型，矮秆 高产
13 21-Y-678 中间型 总状 帚型，矮秆 高产 43 21-Y-808 中间型 圆锥 扇型，矮秆 高产
14 21-Y-679 松散型 圆锥 扇型，矮秆 高产 44 21-Y-814 松散型 圆锥 帚型，高秆 低产
15 21-Y-696 松散型 圆锥 帚型，高秆 高产 45 21-Y-832 松散型 圆锥 帚型，高秆 低产
16 21-Y-700 中间型 总状 帚型，矮秆 低产 46 21-Y-835 松散型 圆锥 帚型，矮秆 高产
17 21-Y-706 中间型 圆锥 帚型，高秆 高产 47 21-Y-837 中间型 圆锥 帚型，矮秆 高产
18 21-Y-708 中间型 圆锥 帚型，矮秆 高产 48 21-Y-844 中间型 圆锥 帚型，矮秆 高产
19 21-Y-714 中间型 圆锥 帚型，矮秆 低产 49 21-Y-858 中间型 圆锥 帚型，矮秆 低产
20 21-Y-716 紧凑型 圆锥 帚型，矮秆 高产 50 21-Y-862 中间型 圆锥 帚型，矮秆 低产
21 21-Y-717 中间型 圆锥 扇型，矮秆 低产 51 21-Y-882 松散型 总状 帚型，高秆 高产
22 21-Y-718 中间型 圆锥 帚型，矮秆 低产 52 21-Y-897 中间型 圆锥 帚型，矮秆 高产
23 21-Y-720 中间型 圆锥 帚型，矮秆 高产 53 21-Y-898 松散型 总状 帚型，高秆 高产
24 21-Y-723 中间型 圆锥 帚型，矮秆 低产 54 21-Y-909 紧凑型 圆锥 帚型，矮秆 高产
25 21-Y-725 中间型 圆锥 帚型，高秆 高产 55 21-Y-924 松散型 圆锥 帚型，矮秆 低产
26 21-Y-732 松散型 圆锥 帚型，矮秆 高产 56 21-Y-925 中间型 圆锥 帚型，矮秆 低产
27 21-Y-737 中间型 圆锥 帚型，矮秆 低产 57 21-Y-926 中间型 圆锥 帚型，矮秆 高产
28 21-Y-743 中间型 圆锥 帚型，高秆 高产 58 21-Y-937 紧凑型 圆锥 帚型，高秆 高产
29 21-Y-761 中间型 圆锥 帚型，矮秆 高产 59 21-Y-954 中间型 圆锥 扇型，矮秆 高产
30 21-Y-765 中间型 圆锥 帚型，高秆 高产 60 21-Y-961 紧凑型 圆锥 扇型，高秆 高产

Table 2

Standards for recording agronomic traits of quinoa"

性状Trait 记录标准Record standard
株高
Plant height (cm)		 成熟期地面至植株顶端的距离
产量Yield (kg) 成熟期籽粒的风干质量
穗色
Ear color		 黄绿=1、玫红=2、黄=3、粉红=4、橘黄=5、绿= 6、红绿=7、紫=8、玫红绿=9、玫红黄=10、白=11
秆色
Stem color
 绿=1、黄绿=2、玫红=3、玫红绿=4、玫红黄=5、黄=6、粉红=7、粉红绿=8、红=9、红绿=10、浅黄=11、红黄绿=12、紫红绿=13
主穗紧凑程度
Compactness
of main ear		 中间型=1、松散型=2、紧凑型=3

株型
Plant type		 帚型、扇型、筒型
花序类型
Inflorescence type		 圆锥花序、总状花序、伞状花序

Fig.1

Electrophoresis results of DNA quality of quinoa resources 1-12 are the random presentation of the test materials"

Table 3

Sequences of SRAP primers"

正向引物Forward primer 序列Sequence (5′-3′) 反向引物Reverse primer 序列Sequence (5′-3′)
A1 TGAGTCCAAACCGGATA B1 GACTGCGTACGAATTAAT
A2 TGAGTCCAAACCGGAGC B2 GACTGCGTACGAATTTGC
A3 TGAGTCCAAACCGGAAT B3 GACTGCGTACGAATTGAC
A4 TGAGTCCAAACCGGAAC B4 GACTGCGTACGAATTTGA
A5 TGAGTCCAAACCGGAAG B5 GACTGCGTACGAATTAAC
A6 TGAGTCCAAACCGGATG B6 GACTGCGTACGAATTGCA
A7 TGAGTCCAAACCGGACT B7 GACTGCGTACGAATTGTA
A8 TGAGTCCAAACCGGTGA B8 GACTGCGTACGAATTACT
A9 TGAGTCCAAACCGGACC B9 GACTGCGTACGAATTGAT
A10 TGAGTCCAAACCGGACT B10 GACTGCGTACGAATTCAA
A11 TGAGTCCAAACCGGACA B11 GACTGCGTACGAATTCAC
A12 TGAGTCCAAACCGGACG B12 GACTGCGTACGAATTCTG
A13 TGAGTCCAAACCGGAGG B13 GACTGCGTACGAATTGTC
A14 TGAGTCCAAACCGGAAA B14 GACTGCGTACGAATTCGA
A15 TGAGTCCAAACCGGAGA B15 GACTGCGTACGAATTAGC
A16 TGAGTCCAAACCGGCAT
A17 TGAGTCCAAACCGGTCC
A18 TGAGTCCAAACCGGTGC

Table 4

Amplification results of SRAP primer combination"

序号
Code		 引物组合
Primer
combination		 总位点数
Number of
total loci		 多态性位点数
Number of
polymorphic loci		 多态性位点百分率
Percentage of
polymorphic loci (%)
1 A4B9 7 7 100.0
2 A4B15 6 6 100.0
3 A5B4 4 4 100.0
4 A6B3 3 3 100.0
5 A9B10 9 8 88.9
6 A9B13 5 4 80.0
7 A11B8 4 3 75.0
8 A11B15 8 8 100.0
9 A14B10 6 5 83.3
10 A14B13 7 7 100.0
11 A17B2 5 5 100.0
总数Total 64 60 93.8
平均数Mean 5.8 5.5 93.4

Table 5

Genetic diversity of quinoa materials"

引物组合
Primer combination		 Na Ne H′ I PIC
A4B9 2.0000 1.4761 0.3048 0.4754 0.3360
A4B15 2.0000 1.6023 0.3523 0.5257 0.3523
A5B4 2.0000 1.3999 0.2492 0.3956 0.2491
A6B3 2.0000 1.7008 0.4011 0.5878 0.4013
A9B10 1.8889 1.4883 0.2759 0.4157 0.2760
A9B13 1.6000 1.3307 0.2012 0.3067 0.2012
A11B8 2.0000 1.5283 0.2853 0.4250 0.2855
A11B15 2.0000 1.5866 0.3438 0.5171 0.3984
A14B10 1.8333 1.6597 0.3393 0.4817 0.3393
A14B13 2.0000 1.6568 0.3783 0.5598 0.3699
A17B2 2.0000 1.4794 0.3096 0.4810 0.3097
平均值Mean 1.9375 1.5384 0.3135 0.4711 0.3199

Fig.2

UPGMA dendrogram of 60 quinoa materials"

Fig.3

Effects of cycle times on SRAP-PCR"

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