Crops ›› 2023, Vol. 39 ›› Issue (6): 160-166.doi: 10.16035/j.issn.1001-7283.2023.06.022

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Study on the Effects of Exogenous Jasmonic Acid on Selenium Tolerance and Selenium Accumulation in Pak Choi (Brassica chinensis L.)

Chen Jinping(), Pan Liping, Xing Ying, Liao Qing, Liu Yongxian, Che Jianglü()   

  1. Agricultural Resources and Environment Research Institute, Guangxi Academy of Agricultural Sciences/ Guangxi Selenium-Rich Agricultural Research Center/Guangxi Selenium-Rich Agricultural Products Engineering Technology Research Center, Nanning 530007, Guangxi, China
  • Received:2022-03-18 Revised:2022-06-07 Online:2023-12-15 Published:2023-12-15

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Abstract:

This study examined the effects of varying exogenous jasmonic acid (JA, 50μmol/L, 100μmol/L) concentrations on the tolerance and accumulation of selenium (Se) in pak choi cabbage (Brassica chinensis L.) treated with various selenium (Se) concentrations (4mg/kg Se6+, 10mg/kg Se6+). The results showed that, plants grown in Se-rich soil treatments exhibited Se toxicity, reduced photosynthetic capacity of photosystem (PSII), heavy peroxidization and significant inhibition of shoot biomass. Under Se-rich soil conditions, pak choi cabbage with 100μmol/L JA treatment resisted the effects of Se toxicity. There were significant increases in the effective quantum yield (Fv'/Fm'), electron transport efficiency (ETR), photochemical quenching coefficient (qP), shoot biomass and catalase (CAT) activity, as well as significant reduced non-photochemical quenching coefficient (qN) and malondialdehyde (MDA) content. Additionally, pak choi cabbage cultivated in Se-rich soil and with 100μmol/L JA treatment showed a significant increase in Se content and Se accumulation of shoots. 50μmol/L JA treatment produced varying degrees of Se tolerance dependent on the concentration of soil Se. Pak choi cabbage in 4mg/kg Se6+ treatment showed reductions in cell peroxidation and inhibition of photochemical reaction, though not significantly. While with 10mg/kg Se6+ treatment, there were significant increases in maximum photochemical efficiency (Fv/Fm), Fv'/Fm', ETR, and CAT activity, though not to the degree of the 100μmol/L JA treatment. Application of 100μmol/L exogenous JA on pak choi cabbage can increase Se tolerance and accumulation ability. It is an effective strategy to lessen Se toxicity and enhance crop health in high-Se containing soil.

Key words: Pak choi cabbage, Selenium-rich soil, Jasmonic acid, Chlorophyll fluorescence, Accumulation of selenium

Table 1

The effects of JA treatment on the chlorophyll fluorescence parameters of pak choi plants in Se-enriched soil"

处理Treatment Fv/Fm Fv'/Fm' ETR qP qN
CK 0.821±0.003abc 0.593±0.005a 118.736±7.324b 0.476±0.032b 0.707±0.029cd
T1 0.812±0.004c 0.533±0.017c 89.960±11.282cd 0.401±0.038c 0.780±0.005a
T2 0.829±0.002a 0.542±0.006bc 86.428±7.198cd 0.379±0.030c 0.784±0.004a
T3 0.818±0.005bc 0.593±0.013a 142.935±12.803a 0.574±0.051a 0.696±0.034d
T4 0.802±0.010d 0.502±0.004d 76.123±4.770d 0.360±0.021c 0.787±0.008a
T5 0.823±0.002ab 0.541±0.008bc 98.313±1.721c 0.432±0.014bc 0.767±0.004ab
T6 0.815±0.002bc 0.561±0.025b 119.038±18.318b 0.504±0.065ab 0.737±0.016bc

Fig.1

Effects of JA treatment on MDA content and antioxidant enzymatic activities of pak choi plants in Se-enriched soil Different lowercase letters are significantly difference at P < 0.05 among different treatments; The F-values of Se×JA interaction on MDA, SOD, POD and CAT were 1.560, 2.904, 1.780 and 5.539, respectively, and the interaction influence on CAT activity is significant (P < 0.05)"

Table 2

Effects of JA treatment on biomass, root-shoot ratio, Se content and Se accumulation of pak choi plants in Se-enriched soil"

处理
Treatment		 地上部生物量
（g/盆）
Shoot biomass
(g/pot)		 根生物量
（g/盆）
Root biomass
(g/pot)		 地上部硒含量
Se content of
shoots (mg/kg)		 根部硒含量
Se content of
roots (mg/kg)		 地上部硒积累量
（μg/盆）
Se accumulation
in shoots (μg/pot)		 根部硒积累量
（μg/盆）
Se accumulation
in roots (μg/pot)		 根冠比
Root-
shoot
ratio
CK 7.17±0.96a 1.74±0.37ab 0.23±0.04e 0.06±0.02d 1.66±0.21d 0.09±0.02e 0.24±0.02a
T1 5.24±0.29b 1.46±0.19b 15.61±2.44d 9.27±1.26c 81.35±8.54c 13.35±0.22d 0.28±0.02a
T2 5.16±0.69b 1.62±0.31ab 17.99±0.73cd 8.56±0.95c 92.57±9.06c 14.10±4.03d 0.32±0.10a
T3 7.04±1.20a 2.01±0.35a 19.92±0.83c 9.42±2.29c 139.60±18.93b 18.48±1.68c 0.29±0.10a
T4 5.17±0.17b 1.78±0.16ab 29.69±0.73b 15.68±1.40b 153.52±2.23b 27.78±1.30b 0.31±0.03a
T5 6.25±0.66ab 1.35±0.05b 37.60±1.76a 20.55±1.45a 234.54±17.88a 27.76±1.08b 0.22±0.03a
T6 7.14±0.06a 1.75±0.27ab 34.98±2.07a 20.87±1.69a 249.93±16.65a 36.15±2.76a 0.24±0.04a
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