Crops ›› 2023, Vol. 39 ›› Issue (6): 150-159.doi: 10.16035/j.issn.1001-7283.2023.06.021

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Ability of DSE against Abiotic Stresses and Improving Drought Resistance of Solanum tuberosum

Bai Jinghua1,2(), Jia Xiaomei1, Wu Yanqing1(), Wang Yuekun1, Song Weiyang1, Liu Yinuo1   

  1. 1College of Biochemical and Environmental Engineering, Baoding University, Baoding 071000, Hebei, China
    2College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China
  • Received:2023-05-05 Revised:2023-09-19 Online:2023-12-15 Published:2023-12-15

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Abstract:

In order to explore the effect of dark septate endophytes (DSE) on growth of potato (Solanum tuberosum), the stress tolerance of Alternaria alternata CTW-9 strain obtained in our lab were tested under ultraviolet ray, different pH, alkaline salt and heat treatments, and then CTW-9 strain was inoculated on potato roots to determine the vegetative growth and drought resistance physiology under different degrees of drought stress. The results showed that the colony diameter and hyphae diameter in the stressed CTW-9 strain was decreased evidently in comparison with the control strain. However, the inhibition of mycelial growth tended to stabilize after a certain decline under treatment with extremely stress conditions. The mycelial elongation growth of treated CTW-9 strains was suppressed markedly, the hyphal septum spacing became shorter than that of control strain, but bold growth was not depressed obviously and partial hyphae formed chlamydospore-like structure, and the strains still could grow as usual. At 30 days after inoculation, it was observed that CTW-9 strain colonized and grown in potato roots. Under different drought stresses, the plant height, fresh weight, dry weight, the relative water content, relative chlorophyll content of leaves, superoxide dismutase and peroxidase activities were increased in comparison with control potato plants. These findings indicate that CTW-9 strain has a strong ability to resist abiotic stresses, and can improve drought resistance significantly of potato plant and promote plant growth.

Key words: Dark septate endophytes, Alternaria alternata, Abiotic stresses, Solanum tuberosum, Drought resistance

Fig.1

Growth state and parameters of CTW-9 under ultraviolet stresses (a) The state of bacterial growth, (b) Growth parameters. t254nm is ultraviolet irradiation time of A254nm"

Table 1

Maximum diameter change models fitting of CTW-9 under different abiotic stresses"

非生物胁迫Abiotic stress 菌落最大直径Maximum colony diameter 菌丝最大直径Maximum hyphae diameter
紫外线处理Ultraviolet treatment y1=-0.3944x13+5.5119x12-24.079x1+61.386（R2=0.947） y2=-2.091ln(x1)+8.1515（R2=0.974）
pH处理pH treatment y1=0.4389x23-5.7833x22+19.263x2+22.8（R2=0.932） y2=-0.213x22+0.9644x2+6.999（R2=0.998）
碱性盐处理Alkaline salt treatment y1=41.711x3-0.226（R2=0.928） y2=7.8267x3-0.172（R2=0.948）
热处理Heat treatment y1=-11.15ln(x4)+44.155（R2=0.967） y2=-1.423ln(x4)+8.1713（R2=0.981）

Fig.2

Growth state and parameters of CTW-9 under extreme pH stresses (a) The state of bacterial growth, (b) Growth parameters. pHPDA is the pH of PDA"

Fig.3

Growth state and parameters of CTW-9 under alkaline salt stresses (a) The state of bacterial growth, (b) Growth parameters. c(NaHCO3) is the concentration of NaHCO3"

Fig.4

Growth state and parameters of CTW-9 under high temperature stresses (a) The state of bacterial growth, (b) Growth parameters. TCTW-9 is the heat treatment temperatures of CTW-9"

Fig.5

Colonization of CTW-9 in roots of potatoes The arrows point to the CTW-9 hyphae at potato roots colonization site"

Fig.6

Effects of CTW-9 on potato growth under drought stress Different letters indicate significant difference under drought stresses (P < 0.05),“*”indicates significant difference between CTW-9 and CK (P < 0.05), the same below"

Fig.7

Effects of CTW-9 on water and chlorophyll contents of potato leaves under drought stress"

Fig.8

Effects of CTW-9 on SOD and POD activities of potato under drought stress"

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