Crops ›› 2024, Vol. 40 ›› Issue (3): 247-251.doi: 10.16035/j.issn.1001-7283.2024.03.033

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Molecular Marker Screening of Potato Late Blight Resistant Gene R8 and Evaluation of Field Resistance

Lou Shubao1,2(), Yang Mengping1, Xing Jinyue1, Zhai Lingxia1, Wang Hui1, Liu Chunsheng1, Wang Lichun1, Song Jiling1()   

  1. 1Keshan Branch of Heilongjiang Academy of Agricultural Sciences / Test-Tube Plantlet Bank of National Potato Germplasm Resources (Keshan), Qiqihar 161005, Heilongjiang, China
    2Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, Qinghai, China
  • Received:2023-03-14 Revised:2023-05-22 Online:2024-06-15 Published:2024-06-18

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Abstract:

Potato is an important food crop that plays an important role in global food security. Late blight caused by Phytophthora infestans is one of the most devastating diseases in potato production, and planting broad-spectrum and durable resistant varieties is the most economical, effective and green way to control late blight. In this study, 102 resources were conduced for R8 gene using specific molecular markers, and 84 potato materials with disease resistance and containing R8 gene were selected in combination with field late blight resistance identification, providing abundant resource materials for selection and breeding of disease resistant varieties. According to the results of molecular marker and field resistance identification, the molecular marker resistance compliance was 84.8%, so the R8 molecular marker could be used for marker-assisted selection.

Key words: Potato, Late blight, Molecular marker, Field resistance

Table 1

R8 gene molecular marker"

基因
Gene		 标记名称
Marker name		 引物序列
Primer sequence (5′-3′)		 退火温度
Annealing temperature (℃)		 产物大小
Product size (bp)
R8 R8-Fd CTGGCGCTGGTTTTGCTATGC 59 682
R8-Rd TCTCTTCGACTTCTTCTTACGAGGTCTA

Fig.1

Evaluation results of field resistance to late blight"

Table 2

Late blight resistance grades and detection of molecular marker"

编号
Number		 品种名称
Variety name		 晚疫病抗性
Late blight resistance		 病情指数
Disease index		 R8基因
R8 gene		 编号
Number		 品种名称
Variety name		 晚疫病抗性
Late blight resistance		 病情指数
Disease index		 R8基因
R8 gene
1 克新25号 中感 55.6 有 52 冀张薯14号 中抗 48.8 有
2 陇薯11号 中抗 33.3 有 53 丽薯1号 中抗 39.2 有
3 维拉斯 中抗 32.3 有 54 华颂1号 高抗 24.5 有
4 内薯7号 中抗 44.3 有 55 晋薯16号 高抗 16.8 有
5 克新26号 中抗 35.7 有 56 华颂88 中抗 41.1 无
6 陇薯6号 中抗 31.3 有 57 丽薯15号 中抗 34.4 有
7 S.jamesii 中抗 44.7 有 58 紫罗兰 高抗 22.3 有
8 丽薯2号 中抗 44.7 有 59 富金 高感 89.6 有
9 S.acaule 高抗 8.3 有 60 垦加3号 中抗 33.2 有
10 陇薯5号 中抗 32.6 有 61 云薯105 高抗 9.3 有
11 延薯12号 高抗 20.5 有 62 北疆1号 中抗 39.2 有
12 延薯1号 中抗 37.7 有 63 同薯29号 中感 60.3 有
13 中薯4号 中感 65.9 有 64 兴佳2号 高感 88.6 有
14 中薯16号 高抗 15.3 有 65 云薯506 高抗 14.1 有
15 蒙薯13号 高抗 24.5 有 66 华恩1号 中抗 44.3 有
16 川芋85 中抗 44.3 无 67 S.stenotomum 高抗 10.0 有
17 延薯10号 中抗 35.1 有 68 希森6号 中抗 45.5 有
18 岭薯1号 中抗 36.7 有 69 土岩2号 中抗 46.1 有
19 丽薯7号 高抗 27.7 有 70 郑薯5号 中抗 37.8 有
20 丽薯6号 中抗 33.3 有 71 兴佳3号 中抗 46.7 有
21 大同里外黄 中抗 40.6 有 72 克新23号 中感 52.2 有
22 韩威1号 中抗 43.5 有 73 费乌瑞它 高感 92.3 有
23 中薯2号 中感 58.5 有 74 云薯202 高抗 19.7 有
24 滇薯47 中抗 35.3 有 75 S.chacoense 中抗 31.5 有
25 韩锦2号 中抗 33.7 有 76 S.stoloniferum 中抗 37.4 有
26 晋薯15号 中抗 44.2 有 77 东薯3号 中抗 33.4 有
27 丽薯11号 高抗 22.2 有 78 晋薯8号 中抗 39.5 有
28 云薯205 中抗 31.4 有 79 云薯305 高抗 17.7 有
29 鄂马铃薯13号 高抗 16.5 有 80 云薯902 高抗 22.3 有
30 宁蒗5号 中抗 37.7 有 81 云薯505 高抗 21.7 有
31 菲勒塞纳 中抗 38.7 有 82 云薯107 中抗 33.7 有
32 丽薯10号 高抗 17.5 有 83 大西洋 高感 94.8 有
33 天薯15号 高抗 21.0 有 84 S.pinnatisecta 高抗 11.1 无
34 泉云3号 中抗 47.7 有 85 陇薯10号 高抗 14.6 有
35 中薯9号 中抗 35.5 有 86 克新34 中抗 37.3 有
36 东薯1号 中抗 40.7 有 87 克新33 高抗 15.6 有
37 华颂11 中抗 34.3 有 88 合作88 高抗 27.7 有
38 垦彩薯1号 中抗 32.3 有 89 庄薯3号 高抗 22.3 有
39 春秋9号 中抗 31.1 有 90 紫花白 高抗 8.9 有
40 冀张薯5号 中抗 44.9 有 91 天薯9号 高抗 23.3 有
41 北薯2号 中抗 45.9 有 92 NS51-5 高抗 23.1 有
42 华颂34 中感 55.7 有 93 米拉 中抗 48.9 有
43 闽薯1号 高感 78.7 有 94 垦薯1号 中抗 33.2 有
44 鑫科薯1号 高抗 13.3 有 95 黑森 高抗 7.5 有
45 宁薯3号 高抗 19.8 有 96 黑美人 高抗 9.8 有
46 中薯6号 中抗 34.2 有 97 克新19号 中感 52.3 有
47 云薯401 高抗 16.4 有 98 天薯10号 中抗 33.2 有
48 中薯15号 中抗 44.6 有 99 克新13号 中抗 46.8 有
49 雪育2号 中抗 37.7 有 100 Atzimba 中抗 44.8 有
50 新大坪 中抗 44.2 有 101 扎列娃 高抗 10.9 有
51 尤金 高感 90.3 有 102 克新1号 中抗 48.8 有

Fig.2

Molecular marker detection of R8 gene M: DL2000 DNA marker; 1: Longshu 6; 2: S.acaule; 3: Lishu 2; 4: Longshu 5; 5: Yanshu 12; 6: Yanshu 1; 7: Zhongshu 4; 8: Zhongshu 16; 9: Mengshu 13; 10: Chuanyu 85; 11: Yanshu 10; 12: Lingshu 1; 13: Lishu 7; 14: Lishu 6; 15: Datongliwaihuang; 16: Hanwei 1."

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